成果/Result
- Effective cancer immunotherapy by Ganoderma lucidum polysaccharide-gold nanocomposites through dendritic cell activation and memory T cell response被引量：122收藏
- 作者：Zhang, Shulei Pang, Guibin Chen, Chao Qin, Jianzhong Yu, Huan Liu, Yongming Zhang, Xihui Song, Zhentao Zhao, Jian Wang, Fujun Wang, Yangyun Zhang, Leshuai W.
- 机构：Shanghai Univ Tradit Chinese Med;Soochow Univ;Changshu 2 Peoples Hosp;Soochow Univ;Zhejiang Reachall Pharmaceut Co Ltd
- 来源：CARBOHYDRATE POLYMERS 2019
- 关键词：Polysaccharides Dendritic cell Nanoparticle Immunoregulation Cancer therapy
- A general approach to the design of high-performance near-infrared (NIR) D-π-A type fluorescent dyes被引量：109收藏
- 作者：Luo, Xiao Li, Jin Zhao, Jie Gu, Luyan Qian, Xuhong Yang, Youjun
- 机构：East China Normal Univ;East China Univ Sci & Technol
- 来源：CHINESE CHEMICAL LETTERS 2019
- 关键词：Near infrared Polymethine cyanine Fluorescence Steric protection Rigidity
- Biochemical properties and potential applications of an organic solvent-tolerant lipase isolated from Serratia marcescens ECU1010被引量：84收藏
- 作者：Zhao, Li-Li Xu, Jian-He Zhao, Jian Pan, Jiang Wang, Zhi-Long
- 机构：E China Univ Sci & Technol;Shanghai Jiao Tong Univ
- 来源：PROCESS BIOCHEMISTRY 2008
- 关键词：lipase Serratia marcescens organic solvent tolerance enantiomeric resolution chiral compounds biochemical properties
- Comparison of immunoregulatory effects of polysaccharides from three natural herbs and cellular uptake in dendritic cells被引量：67收藏
- 作者：Zhu, Ning Lv, Xiaochen Wang, Yangyun Li, Jingling Liu, Yongming Lu, Wenfei Yang, Liecheng Zhao, Jian Wang, Fujun Zhang, Leshuai W.
- 机构：Shanghai Univ Tradit Chinese Med;Soochow Univ;Soochow Univ;Zhejiang Reachall Pharmaceut Co Ltd;East China Univ Sci & Technol
- 来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2016
- 关键词：Natural herbs Polysaccharides Dendritic cell Nitric oxide Endocytosis
- Comparative studies on the immunoregulatory effects of three polysaccharides using high content imaging system被引量：53收藏
- 作者：Lv, Xiaocheng Chen, Dandan Yang, Liecheng Zhu, Ning Li, Jingling Zhao, Jian Hua, Zhibi Wang, Fu-Jun Zhang, Leshuai W.
- 机构：Shanghai Univ Tradit Chinese Med;Soochow Univ;Zhejiang Reachall Pharmaceut Co Ltd;E China Univ Sci & Technol;Shanghai R&D Ctr Standardizat Chinese Med
- 来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2016
- 关键词：Polysaccharide Natural products Immunoregulation Cell irregularity Nitrogen oxide Macrophage
- The protective role of autophagy in nephrotoxicity induced by bismuth nanoparticles through AMPK/mTOR pathway被引量：47收藏
- 作者：Liu, Yongming Yu, Huan Zhang, Xihui Wang, Yong Song, Zhentao Zhao, Jian Shi, Haibin Li, Ruibin Wang, Yangyun Zhang, Leshuai W.
- 机构：Soochow Univ;Soochow Univ;East China Univ Sci & Technol
- 来源：NANOTOXICOLOGY 2018
- 关键词：Bismuth CT imaging acute kidney injury autophagy cell signaling pathway
- An Unusual (R)-Selective Epoxide Hydrolase with High Activity for Facile Preparation of Enantiopure Glycidyl Ethers被引量：45收藏
- 作者：Zhao, Jing Chu, Yan-Yan Li, Ai-Tao Ju, Xin Kong, Xu-Dong Pan, Jiang Tang, Yun Xu, Jian-He
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol
- 来源：ADVANCED SYNTHESIS & CATALYSIS 2011
- 关键词：enantioselectivity epoxide hydrolase glycidyl ethers kinetic resolution molecular modeling
- Expression and Characterization of a Novel Lipase from Aspergillus fumigatus with High Specific Activity被引量：31收藏
- 作者：Shangguan, Jiao-Jiao Liu, Yu-Qiang Wang, Fu-Jun Zhao, Jian Fan, Li-Qiang Li, Su-Xia Xu, Jian-He
- 机构：E China Univ Sci & Technol;Shanghai Univ Chinese Tradit Med
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2011
- 关键词：Aspergillus fumigatus Recombinant lipase Expression Characterization Active site
- Enhancement of cellulase production in Trichoderma reesei RUT-C30 by comparative genomic screening被引量：31收藏
- 作者：Liu, Pei Lin, Aibo Zhang, Guoxiu Zhang, Jiajia Chen, Yumeng Shen, Tao Zhao, Jian Wei, Dongzhi Wang, Wei
- 机构：East China Univ Sci & Technol;Sunson Ind Grp Co Ltd
- 来源：MICROBIAL CELL FACTORIES 2019
- 关键词：Trichoderma reesei RUT-C30 Genome sequencing Cellulase production tre108642 tre56839 Alcohol dehydrogenase CRE1
- Heterologous overexpression of Vigna radiata epoxide hydrolase in Escherichia coli and its catalytic performance in enantioconvergent hydrolysis of p-nitrostyrene oxide into (R)-p-nitrophenyl glycol被引量：29收藏
- 作者：Zhu, Qing-Qing He, Wan-Hong Kong, Xu-Dong Fan, Li-Qiang Zhao, Jian Li, Su-Xia Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2014
- 关键词：RT-PCR Vigna radiata epoxide hydrolase p-Nitrostyrene oxide Enantioconvergency Characterization
- Switching a Newly Discovered Lactonase into an Efficient and Thermostable Phosphotriesterase by Simple Double Mutations His250Ile/Ile263Trp被引量：28收藏
- 作者：Luo, Xiao-Jing Kong, Xu-Dong Zhao, Jian Chen, Qi Zhou, Jiahai Xu, Jian-He
- 机构：E China Univ Sci & Technol;Chinese Acad Sci
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2014
- 关键词：organophosphate phosphotriesterase lactonase site-directed mutagenesis functional switch X-ray crystallography
- Effective Therapeutic Drug Delivery by GALA3, an Endosomal Escape Peptide with Reduced Hydrophobicity被引量：25收藏
- 作者：Li, Chen Cao, Xue-Wei Zhao, Jian Wang, Fu-Jun
- 机构：East China Univ Sci & Technol;Zhejiang Fonow Med Co Ltd;Shanghai R&D Ctr Standardizat Chinese Med;Shanghai Univ Tradit Chinese Med;East China Univ Sci & Technol
- 来源：JOURNAL OF MEMBRANE BIOLOGY 2020
- 关键词：Endosomal escape HBP GALA3 BLF1
- Combinatorial Evolution of Phosphotriesterase Toward a Robust Malathion Degrader by Hierarchical Iteration Mutagenesis被引量：25收藏
- 作者：Luo, Xiao-Jing Zhao, Jian Li, Chun-Xiu Bai, Yun-Peng Reetz, Manfred T. Yu, Hui-Lei Xu, Jian-He
- 机构：East China Univ Sci & Technol;Max Planck Inst Kohlenforsch;Philipps Univ Marburg
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2016
- 关键词：biocatalysis directed evolution DNA shuffling malathion phosphotriesterase
- Recombinant expression and purification of a MAP30-cell penetrating peptide fusion protein with higher anti-tumor bioactivity被引量：23收藏
- 作者：Lv, Qiang Yang, Xu-Zhong Fu, Long-Yun Lu, Yv-Ting Lu, Yan-Hua Zhao, Jian Wang, Fu-Jun
- 机构：E China Univ Sci & Technol;Zhejiang Reachall Pharmaceut Co Ltd;Shanghai Univ Chinese Tradit Med
- 来源：PROTEIN EXPRESSION AND PURIFICATION 2015
- 关键词：Recombinant Momordica Antiviral Protein 30 Kd (rMAP30) Ribosome inactivating protein Cell Penetrating Peptide Ni-NTA purification Anti-tumor activity
- Engineering Escherichia coli for l-homoserine production被引量：22收藏
- 作者：Sun, Bing-Yao Wang, Feng-Qing Zhao, Jian Tao, Xin-Yi Liu, Min Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BASIC MICROBIOLOGY 2023
- 关键词：Escherichia coli hok sok system l-homoserine metabolic engineering plasmid stability
- Analysis of Triterpenoid Saponins Reveals Insights into Structural Features Associated with Potent Protein Drug Enhancement Effects被引量：22收藏
- 作者：Cao, Xue-Wei Wang, Fu-Jun Liew, Oi-Wah Lu, Ye-Zhou Zhao, Jian
- 机构：Zhejiang Fonow Med Co Ltd;Shanghai R&D Ctr Standardizat Chinese Med;Shanghai Univ Tradit Chinese Med;East China Univ Sci & Technol;Ctr Translat Med
- 来源：MOLECULAR PHARMACEUTICS 2020
- 关键词：saponins endosomal escape enhancers (EEEs) glycosylated triterpenoid ribosome-inactivating proteins (RIPS) cytosolic drug delivery drug release
- A novel human derived cell-penetrating peptide in drug delivery被引量：20收藏
- 作者：Zhao, Jian Gao, Peng Xiao, Wei Fan, Li-Qiang Wang, Fu-Jun Li, Su-Xia Liu, Jian-Wen
- 机构：E China Univ Sci & Technol;Zhejiang Reachall Pharmaceut Co Ltd
- 来源：MOLECULAR BIOLOGY REPORTS 2011
- 关键词：hC-SOD3 Human cell-penetrating peptide Apoptin Drug delivery
- Recent research advancements on regioselective nitrilase: fundamental and applicative aspects被引量：19收藏
- 作者：Chen, Zhi Zhao, Jiang Jiang, Shuiqing Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2019
- 关键词：Biocatalysis Regioselective nitrilase Regioselectivity Molecular modifications Industrial applications
- Spectral and biodistributional engineering of deep near-infrared chromophore被引量：19收藏
- 作者：Dong, Yan Lu, Xicun Li, Yi Chen, Weichao Yin, Lei Zhao, Jie Hu, Xinru Li, Xinran Lei, Zuhai Wu, Yuyang Chen, Hao Luo, Xiao Qian, Xuhong Yang, Youjun
- 机构：East China Univ Sci & Technol;East China Normal Univ;Chinese Acad Sci
- 来源：CHINESE CHEMICAL LETTERS 2023
- 关键词：Near infrared fluorophore In vivo imaging Molecular engineering Biological distribution Angiography
- Gene cloning and expression of a novel hypoglycaemic peptide from Momordica charantia被引量：18收藏
- 作者：Wang, Bi-lian Zhang, Wen-ji Zhao, Jian Wang, Fu-jun Fan, Li-qiang Wu, Yi-xin Hu, Zhi-bi
- 机构：E China Univ Sci & Technol;Shanghai Univ Chinese Tradit Med;Hamamatsu Univ Sch Med
- 来源：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE 2011
- 关键词：Momordica charantia gene cloning polypeptide-P hypoglycaemic effect
- Structure-guided engineering of a flavin-containing monooxygenase for the efficient production of indirubin被引量：17收藏
- 作者：Sun, Bing-Yao Sui, Hua-Lu Liu, Zi-Wei Tao, Xin-Yi Gao, Bei Zhao, Ming Ma, Yu-Shu Zhao, Jian Liu, Min Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2022
- 关键词：Flavin-containing monooxygenase Indirubin Loop region Substrate tunnel Structure-guided enzyme engineering Microbial synthesis
- Significantly Improved Expression and Biochemical Properties of Recombinant Serratia marcescens Lipase as Robust Biocatalyst for Kinetic Resolution of Chiral Ester被引量：16收藏
- 作者：Wang, Yi Zhao, Jian Xu, Jian-He Fan, Li-Qiang Li, Su-Xia Zhao, Li-Li Mao, Xiao-Bo
- 机构：E China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2010
- 关键词：Serratia marcescens ECU1010 Lipase Soluble expression Purification Characterization Enantioselectivity Biocatalytic resolution
- Statistical Optimizing of Medium for Clavulanic Acid Production by Streptomyces clavuligerus Using Response Surface Methodology被引量：15收藏
- 作者：Feng, Tao Zhao, Jie Chu, Ju Wang, Yong-hong Zhuang, Ying-ping
- 机构：East China Univ Sci & Technol;Sinopharm Weiqida Pharmaceut Co Ltd
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2021
- 关键词：Clavulanic acid Streptomyces clavuligerus Statistical optimization Response surface methodology
- Tandem Multimer Expression and Preparation of Hypoglycemic Peptide MC6 from Momordica charantia in Escherichia coli被引量：14收藏
- 作者：Wang, Fu-Jun Song, Huai-Lei Wang, Xiao-Meng Zhang, Wen-Ji Wang, Bi-Lian Zhao, Jian Hu, Zhi-Bi
- 机构：E China Univ Sci & Technol;Shanghai Univ Chinese Tradit Med;Shanghai Jiao Tong Univ
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2012
- 关键词：Momordica charantia Hypoglycemic peptide MC6 Multimer Isocaudamer
- Screening novel β-galactosidases from a sequence-based metagenome and characterization of an alkaline β-galactosidase for the enzymatic synthesis of galactooligosaccharides被引量：14收藏
- 作者：Liu, Pei Wang, Wei Zhao, Jian Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：PROTEIN EXPRESSION AND PURIFICATION 2019
- 关键词：beta-Galactosidase CRISPR Cas9 Galactooligosaccharides lacZ Metagenome Transglycosylation
- Statistical optimization of medium for the production of pyruvate oxidase by the recombinant Escherichia coli被引量：14收藏
- 作者：Zhao, Jie Wang, Yonghong Chu, Ju Zhang, Siliang Zhuang, Yingping Yuan, Zhongyi
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2008
- 关键词：pyruvate oxidase recombinant E coli medium optimization response surface methodology
- Enhanced anti-tumor activity of trichosanthin after combination with a human-derived cell-penetrating peptide, and a possible mechanism of activity被引量：13收藏
- 作者：Lu, Ye-Zhou Li, Peng Fei Li, Yan-Zhong Luo, Fan Guo, Chao Lin, Bin Cao, Xue-Wei Zhao, Jian Wang, Fu-Jun
- 机构：East China Univ Sci & Technol;Shanghai Univ Tradit Chinese Med;Zhejiang Reachall Pharmaceut Co Ltd
- 来源：FITOTERAPIA 2016
- 关键词：Trichosanthin Cell-penetrating peptide Tumor therapy Apoptosis
- Enhancing tumor-specific intracellular delivering efficiency of cell-penetrating peptide by fusion with a peptide targeting to EGFR被引量：13收藏
- 作者：Long The Nguyen Yang, Xu-Zhong Du, Xuan Wang, Jia-Wei Zhang, Rui Zhao, Jian Wang, Fu-Jun Dong, Yang Li, Peng-Fei
- 机构：E China Univ Sci & Technol;Zhejiang Reachall Pharmaceut Co Ltd;Shanghai Univ Tradit Chinese Med
- 来源：AMINO ACIDS 2015
- 关键词：Cell-penetrating peptide Tumor-homing peptide Targeted drug delivery Growth factor
- Facile Synthesis of Enantiopure 4-Substituted 2-Hydroxy-4-butyrolactones using a Robust Fusarium Lactonase被引量：13收藏
- 作者：Chen, Bing Yin, Hai-Feng Wang, Zhen-Sheng Xu, Jian-He Fan, Li-Qiang Zhao, Jian
- 机构：E China Univ Sci & Technol;PepTech Corp Shanghai
- 来源：ADVANCED SYNTHESIS & CATALYSIS 2009
- 关键词：chemo-enzymatic process circular dichroism Fusarium proliferatum lactonase stereoselectivity 4-substituted 2-hydroxy-4-butyrolactones
- Biocatalytic Properties of a Recombinant Fusarium proliferatum Lactonase with Significantly Enhanced Production by Optimal Expression in Escherichia coli被引量：12收藏
- 作者：Chen, Bing Fan, Li-Qiang Xu, Jian-He Zhao, Jian Zhang, Xian Ouyang, Li-Ming
- 机构：E China Univ Chem Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2010
- 关键词：Levo-lactonase DL-pantoyl lactone Recombinant protein Immobilized metal affinity chromatography Biocatalytic property Substrate specificity
