成果/Result
- A Highly Efficient Catalyst toward Oxygen Reduction Reaction in Neutral Media for Microbial Fuel Cells被引量：86收藏
- 作者：Su, Yunhe Zhu, Yihua Yang, Xiaoling Shen, Jianhua Lu, Jindan Zhang, Xiaoyan Chen, Jianding Li, Chunzhong
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol
- 来源：INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH 2013
- 关键词：Nitrogen - Cathodes - Nanocomposites - Oxygen - Electrolytes - Electrolytic reduction - Catalyst activity - Doping (additives) - Graphene
- Newly Identified Thermostable Esterase from Sulfobacillus acidophilus: Properties and Performance in Phthalate Ester Degradation被引量：77收藏
- 作者：Zhang, Xiao-Yan Fan, Xiang Qiu, Yong-Jun Li, Cheng-Yuan Xing, Shuai Zheng, Yi-Tao Xu, Jian-He
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2014
- 关键词：Escherichia coli - Esters - Hydrolysis
- Biodegradation of Structurally Diverse Phthalate Esters by a Newly Identified Esterase with Catalytic Activity toward Di(2-ethylhexyl) Phthalate被引量：75收藏
- 作者：Huang, Han Zhang, Xiao-Yan Chen, Tian-Lei Zhao, Yu-Lian Xu, Dian-Sheng Bai, Yun-Peng
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2019
- 关键词：Gordonia polyisoprenivorans esterase bis(2-ethylhexyl) phthalate phthalate esters biodegradation
- Access to Optically Active Aryl Halohydrins Using a Substrate-Tolerant Carbonyl Reductase Discovered from Kluyveromyces thermotolerans被引量：71收藏
- 作者：Xu, Guo-Chao Yu, Hui-Lei Zhang, Xiao-Yan Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：ACS CATALYSIS 2012
- 关键词：carbonyl reductase asymmetric hydrogen transfer aryl halohydrin Kluyveromyces thermotolerans genome data mining substrate/product tolerance
- Access to optically active aryl halohydrins using a substrate-tolerant carbonyl reductase discovered from kluyveromyces thermotolerans被引量：59收藏
- 作者：Xu, Guo-Chao Yu, Hui-Lei Zhang, Xiao-Yan Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology
- 来源：ACS Catalysis 2012
- 关键词：Data mining - Escherichia coli - Enzyme activity - Genes - Ketones
- Coimmobilized Dual Enzymes in a Continuous Flow Reactor for the Efficient Synthesis of Optically Pure γ/δ-Lactones被引量：56收藏
- 作者：Yuan, Pengyu Wang, Qing Deng, Xuelei Zhang, Xiaoyan Fan, Daidi Bai, Yunpeng
- 机构： State Key Laboratory of Bioreactor Engineering; Shaanxi R&D Centre of Biomaterials and Fermentation Engineering
- 来源：ACS Applied Materials and Interfaces 2025
- 关键词：Doping (additives) - Green Synthesis - Reaction rates - Stereoselectivity
- Confining Enzyme Clusters in Bacteriophage P22 Enhances Cofactor Recycling and Stereoselectivity for Chiral Alcohol Synthesis被引量：40收藏
- 作者：Zhang, Yan-Qing Feng, Tao-Tao Cao, Yu-Fei Zhang, Xiao-Yan Wang, Tao Nina, Mario Roque Huanca Wang, Li-Cheng Yu, Hui-Lei Xu, Jian-He Ge, Jun Bai, Yun-Peng
- 机构：East China Univ Sci & Technol ECUST;Tsinghua Univ;Tsinghua Shenzhen Int Grad Sch;Shenzhen Bay Lab;Tsinghua Univ
- 来源：ACS CATALYSIS 2021
- 关键词：enzyme cluster enzymatic catalysis cofactor recycling confinement effects asymmetric reduction
- Transcription Factor Engineering for High-Throughput Strain Evolution and Organic Acid Bioproduction: A Review被引量：37收藏
- 作者：Li, Jia-Wei Zhang, Xiao-Yan Wu, Hui Bai, Yun-Peng
- 机构：East China Univ Sci & Technol
- 来源：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 2020
- 关键词：transcription factor biosensor metabolic engineering synthetic biology organic acid high-throughput screening
- Efficient Stereoselective Synthesis of Structurally Diverse γ- and δ-Lactones Using an Engineered Carbonyl Reductase被引量：26收藏
- 作者：Chen, Meng Zhang, Xiao-Yan Xing, Chen-Guang Zhang, Chao Zheng, Yu-Cong Pan, Jiang Xu, Jian-He Bai, Yun-Peng
- 机构：East China Univ Sci & Technol;Xiamen Oamic Biotech Co Ltd
- 来源：CHEMCATCHEM 2019
- 关键词：Asymmetric reduction directed evolution chiral lactones carbonyl reductase biocatalysis
- An engineered quorum-sensing-based whole-cell biosensor for active degradation of organophosphates被引量：25收藏
- 作者：He, Jianwei Zhang, Xiaoyan Qian, Yuanyi Wang, Qiyao Bai, Yunpeng
- 机构：East China Univ Sci & Technol
- 来源：BIOSENSORS & BIOELECTRONICS 2022
- 关键词：Whole-cell biosensor Synthetic biology Genetic circuits Quorum sensing Degradation
- Engineering Transcription Factor XylS for Sensing Phthalic Acid and Terephthalic Acid: An Application for Enzyme Evolution被引量：23收藏
- 作者：Li, Jiawei Nina, Mario Roque Huanca Zhang, Xiaoyan Bai, Yunpeng
- 机构：East China Univ Sci & Technol
- 来源：ACS SYNTHETIC BIOLOGY 2022
- 关键词：biosensor transcription factor engineering phthalic acid terephthalic acid enzyme evolution biodegradation
- Fabrication of emulsion-templated macroporous poly(ε-caprolactone) towards highly effective and sustainable oil/water separation被引量：19收藏
- 作者：Yang, Xiaocang Yin, Zhengqiao Zhang, Xiaoyu Zhu, Yun Zhang, Shengmiao
- 机构：East China Univ Sci & Technol
- 来源：POLYMER 2020
- 关键词：High internal phase emulsion Poly(epsilon-caprolactone) Water/oil separation
- Continuous-flow enzymatic synthesis of chiral lactones in a three-dimensional microfluidic reactor被引量：18收藏
- 作者：Deng, Xuelei Fan, Meng Wu, Miao Zhang, Xiaoyan Cheng, Ya Xia, Jianye Zhuang, Yingping Zhu, Weiping Qian, Xuhong Bai, Yunpeng
- 机构：East China Univ Sci & Technol;East China Normal Univ;Chinese Acad Sci
- 来源：CHINESE CHEMICAL LETTERS 2024
- 关键词：Continuous-flow Flow chemistry Microreactor Biocatalysis Carbonyl reductase Asymmetric reduction
- Cross-linked enzyme-polymer conjugates with excellent stability and detergent-enhanced activity for efficient organophosphate degradation被引量：17收藏
- 作者：Cheng, Huan Zhao, Yu-Lian Luo, Xiao-Jing Xu, Dian-Sheng Cao, Xun Xu, Jian-He Dai, Qing Zhang, Xiao-Yan Ge, Jun Bai, Yun-Peng
- 机构：East China Univ Sci & Technol;Tsinghua Univ;Natl Ctr Nanosci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2018
- 关键词：Organophosphate Organophosphate hydrolase Immobilization Biodegradation Enzyme-polymer conjugates Pluronic F127
- Continuous-flow synthesis of polysubstituted γ -butyrolactones via enzymatic cascade catalysis被引量：16收藏
- 作者：Chu, Liliang Zhang, Xiaoyan Li, Jianing Deng, Xuelei Wu, Miao Cheng, Ya Zhu, Weiping Qian, Xuhong Bai, Yunpeng
- 机构：East China Univ Sci & Technol;East China Normal Univ
- 来源：CHINESE CHEMICAL LETTERS 2024
- 关键词：Enzyme catalysis Cascade catalysis Directed evolution Continuous-flow gamma -Butyrolactones
- Stereoselective synthesis of chiral δ-lactones via an engineered carbonyl reductase被引量：14收藏
- 作者：Wang, Tao Zhang, Xiao-Yan Zheng, Yu-Cong Bai, Yun-Peng
- 机构：East China Univ Sci & Technol
- 来源：CHEMICAL COMMUNICATIONS 2021
- 关键词：Stereoselectivity - Cost effectiveness - Substrates - Esters
- Direct Access to Medium-Chain α,ω-Dicarboxylic Acids by Using a Baeyer-Villiger Monooxygenase of Abnormal Regioselectivity被引量：14收藏
- 作者：Yu, Jia-Mei Liu, Yuan-Yang Zheng, Yu-Cong Li, Hao Zhang, Xiao-Yan Zheng, Gao-Wei Li, Chun-Xiu Bai, Yun-Peng Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：CHEMBIOCHEM 2018
- 关键词：biocatalysis dicarboxylic acids oxidation regioselectivity renewable resources
- An Orthogonal CRISPR/dCas12a System for RNA Imaging in Live Cells被引量：13收藏
- 作者：Jia, Hai-Yan Zhang, Xin-Yue Ye, Bang-Ce Yin, Bin-Cheng
- 机构：East China Univ Sci & Technol;Shihezi Univ
- 来源：ANALYTICAL CHEMISTRY 2024
- 关键词：Oligonucleotides
- Green access to chiral Vince lactam in a buffer-free aqueous system using a newly identified substrate-tolerant (-)-γ-lactamase被引量：13收藏
- 作者：Yin, Jin-Gang Gong, Yi Zhang, Xiao-Yan Zheng, Gao-Wei Xu, Jian-He
- 机构：East China Univ Sci & Technol
- 来源：CATALYSIS SCIENCE & TECHNOLOGY 2016
- 关键词：Data mining
- Paper-Based Enzymatic Colorimetric Assay for Rapid Malathion Detection被引量：13收藏
- 作者：Li, Jian-Hui Deng, Xue-Lei Zhao, Yu-Lian Zhang, Xiao-Yan Bai, Yun-Peng
- 机构：Ocean Univ China;East China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2021
- 关键词：Enzyme immobilization Paper-based biosensor Malathion detection Polymer– enzyme conjugate Organophosphate hydrolase
- Reshaping the active pocket of promiscuous lactonases for degrading bulky organophosphate flame retardants被引量：12收藏
- 作者：Zhang, Yuanyuan Zhang, Xiaoyan Cheng, Huan Nina, Mario Roque Huanca Ge, Jun Bai, Yunpeng
- 机构：East China Univ Sci & Technol;Tsinghua Univ
- 来源：CHEMICAL COMMUNICATIONS 2021
- 关键词：Flame retardants
- Performance of a New Thermostable Mannanase in Breaking Guar-Based Fracturing Fluids at High Temperatures with Little Premature Degradation被引量：12收藏
- 作者：Hu, Ke Li, Chun-Xiu Pan, Jiang Ni, Yan Zhang, Xiao-Yan Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2014
- 关键词：DtManB Fracturing fluids Mannanase Hydroxypropyl guar Viscosity reduction
- Using algae cells to drive cofactor regeneration and asymmetric reduction for the synthesis of chiral chemicals被引量：11收藏
- 作者：Zheng, Lvhong Zhang, Xiaoyan Bai, Yunpeng Fan, Jianhua
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 2018
- 关键词：Algae biocatalyst Chiral alcohols Biocatalysis Cell factory Microalgae Artificial modification
- Engineering the methyltransferase through inactivation of the genK and genL leads to a significant increase of gentamicin C1a production in an industrial strain of Micromonospora echinospora 49-92S被引量：10收藏
- 作者：Xu, Feng Zhang, Xinyu Liu, Ling Ke, Xiang Wu, Jie Guo, Yuanxin Tian, Xiwei Chu, Ju
- 机构：East China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2022
- 关键词：Micromonospora echinospora Gentamicin C1a Methyltransferase GenK-genL Metabolic flux analysis
- Coimmobilized Dual Enzymes in a Continuous Flow Reactor for the Efficient Synthesis of Optically Pure γ/δ-Lactones被引量：10收藏
- 作者：Yuan, Pengyu Wang, Qing Deng, Xuelei Zhang, Xiaoyan Fan, Daidi Bai, Yunpeng
- 机构：East China Univ Sci & Technol;Northwest Univ
- 来源：ACS APPLIED MATERIALS & INTERFACES 2024
- 关键词：enzyme catalysis enzyme immobilization cofactorrecycling continuous-flow microreactor
- Three new LmbU targets outside lmb cluster inhibit lincomycin biosynthesis in Streptomyces lincolnensis被引量：10收藏
- 作者：Mao, Yue Zhang, Xianyan Zhou, Tianyu Hou, Bingbing Ye, Jiang Wu, Haizhen Wang, Ruida Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：MICROBIAL CELL FACTORIES 2024
- 关键词：Streptomyces lincolnensis Lincomycin biosynthesis Transcriptional regulation LmbU Cluster-situated regulatory gene(s)
- 重组羧肽酶B在胰岛素原C肽制备工艺中的应用被引量：9收藏
- 作者：李素霞 田丽萍 张晓彦 夏文超 龚毅 袁勤生
- 机构：华东理工大学生物反应器工程国家重点实验室;中国科学院上海生命科学研究院生物工程中心
- 来源：《华东理工大学学报（自然科学版）》 2004
- 关键词：羧肽酶原B 羧肽酶B 融合表达 分离纯化 胰岛素原C肽
- 摘要：用RT-PCR方法克隆了大鼠羧肽酶原B的cDNA编码序列,将其重组到原核表达质粒pT7-473中,并在大肠杆菌中以包涵体方式获得高表达。SDS-PAGE电泳及灰度扫描显示表达量达菌体总蛋白的35%,表达产物融合了表达质粒...
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- Mg^(2+)和氨基酸对重组大肠杆菌BL21(DE3)生长及羧肽酶原B表达的影响被引量：9收藏
- 作者：张文超 陈长华 李素霞 张晓彦
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《中国生物制品学杂志》 2006
- 关键词：羧肽酶原B 重组大肠杆菌 发酵 表达
- 摘要：目的研究Mg2+和氨基酸对重组菌株生长及表达pCPB的影响。方法通过摇瓶和发酵罐培养,观察不同Mg2+浓度和氨基酸对重组菌的生长和羧肽酶原B表达的影响。结果添加1g/L Mg2+能提高质粒的稳定性;添加氨基酸能使羧肽酶原...
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- Targeted Heating of Enzyme Systems Based on Photothermal Materials被引量：8收藏
- 作者：Wang, Xuerui Ou, Gang Zhou, Ke Wang, Xiangqing Wang, Licheng Zhang, Xiaoyan Feng, Yi Bai, Yunpeng Wu, Hui Xu, Zhiping Ge, Jun
- 机构：Tsinghua Univ;Tsinghua Univ;Tsinghua Univ;Tsinghua Univ;East China Univ Sci & Technol
- 来源：CHEMBIOCHEM 2019
- 关键词：biosensors enzymes magnetic properties nanoparticles photochemistry
- 从兴趣出发,以OBE理念打造充满魅力的微生物学课堂教学被引量：8收藏
- 作者：张晓彦 叶蕊芳 高淑红 白云鹏 王启要
- 机构：华东理工大学生物工程学院
- 来源：《化工高等教育》 2021
- 关键词：微生物 课堂教学 OBE 启发式 联想式 实践教学
- 摘要：针对微生物学课程,我们基于OBE理念,大力推行启发式和联想式教学,加强师生互动,营造轻松活泼、充满魅力的课堂氛围,引导学生从“要我学”的被动学习向“我要学、我爱学”的主动学习和自主学习转变。教学改革的实施大大提高了学生对...
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