成果/Result
- Biomanufacturing of Biobased Polymers被引量：337收藏
- 作者：Luo, Yuanchan Wu, Ju Fei, Peng Li, Jianing Xu, Mingcheng Guo, Pengye Zhao, Fei Qin, Zhitian Wu, Hui Fan, Liqiang Wang, Yibing Chen, Yijia Shi, Jinqi Zhao, Liming
- 机构： East China University of Science and Technology; State Key Laboratory of Bioreactor Engineering; MOE Key Laboratory of Bio-Intelligent Manufacturing
- 来源：Research and Applications of Bio-based Degradable Materials 2025
- 关键词：Biological materials - Natural resources
- Antioxidant properties of a vegetable-fruit beverage fermented with two Lactobacillus plantarum strains被引量：85收藏
- 作者：Yang, Xiaoxing Zhou, Jiachun Fan, Liqiang Qin, Zhen Chen, Qiming Zhao, Liming
- 机构：East China Univ Sci & Technol;SCICBT
- 来源：FOOD SCIENCE AND BIOTECHNOLOGY 2018
- 关键词：Lactobacillus plantarum Vegetable-fruit beverage Fermentation Metabolites Antioxidant activities
- Progress in the biosynthesis of bio-based PET and PEF polyester monomers被引量：76收藏
- 作者：Cui, Yanan Deng, Chen Fan, Liqiang Qiu, Yongjun Zhao, Liming
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol SCI
- 来源：GREEN CHEMISTRY 2023
- 关键词：Biochemistry - Biomass - Biosynthesis - Conservation - Cost effectiveness - Enzymes - Ethylene - Monomers - Natural resources - Petroleum industry - Plastic bottles - Polyethylene terephthalates
- Chitooligosaccharides display anti-tumor effects against human cervical cancer cells via the apoptotic and autophagic pathways被引量：57收藏
- 作者：Zhao, Mengyao Gu, Liming Li, Yun Chen, Shumin You, Jiangshan Fan, Liqiang Wang, Yudong Zhao, Liming
- 机构：East China Univ Sci & Technol;Shanghai Jiao Tong Univ;SCICBT
- 来源：CARBOHYDRATE POLYMERS 2019
- 关键词：Chitooligosaccharides Enzyme-membrane coupling reactor Apoptosis ROS production Autophagy
- Insights into the nanofiltration separation mechanism of monosaccharides by molecular dynamics simulation被引量：49收藏
- 作者：Yao, Lei Qin, Zhen Chen, Qiming Zhao, Mengyao Zhao, Hefei Ahmad, Waheed Fan, Liqiang Zhao, Liming
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol SC;Univ Nebraska
- 来源：SEPARATION AND PURIFICATION TECHNOLOGY 2018
- 关键词：Monosaccharide Nanofiltration Molecular dynamics simulation Diffusion coefficient Interaction energy
- Investigating the mechanism of nanofiltration separation of glucosamine hydrochloride and N-acetyl glucosamine被引量：44收藏
- 作者：Zhang, Shengnan Zhou, Jiachun Fan, Liqiang Qiu, Yongjun Jiang, Lihua Zhao, Liming
- 机构： State Key Laboratory of Bioreactor Engineering; Shanghai Collaborative Innovation Center for Biomanufacturing Technology
- 来源：Bioresources and Bioprocessing 2016
- 关键词：Bioactivity - Chemical reactions - Chitin - Efficiency - Electrolytes - Glucosamine - Magnesium compounds - Sodium chloride - Temperature
- High level production of a Bacillus amlyoliquefaciens chitosanase in Pichia pastoris suitable for chitooligosaccharides preparation被引量：41收藏
- 作者：Luo, Sa Qin, Zhen Chen, Qiming Fan, Liqiang Jiang, Lihua Zhao, Liming
- 机构：East China Univ Sci & Technol;SCICBT
- 来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2020
- 关键词：Chitosanase Chitooligosaccharides Glycoside hydmlase family 46
- Regioselectivity Engineering of Epoxide Hydrolase: Near-Perfect Enantioconvergence through a Single Site Mutation被引量：41收藏
- 作者：Li, Fu-Long Kong, Xu-Dong Chen, Qi Zheng, Yu-Cong Xu, Qin Chen, Fei-Fei Fan, Li-Qiang Lin, Guo-Qiang Zhou, Jiahai Yu, Hui-Lei Xu, Jian-He
- 机构：East China Univ Sci & Technol;Chinese Acad Sci;Shanghai Jiao Tong Univ;Shanghai Jiao Tong Univ
- 来源：ACS CATALYSIS 2018
- 关键词：biocatalysis epoxide hydrolase crystal structure regioselectivity enantioconvergence protein engineering
- Refolding, purification and characterization of an organic solvent-tolerant lipase from Serratia marcescens ECU1010被引量：37收藏
- 作者：Li, Suxia Pang, Huaiyu Lin, Kang Xu, Jianhe Zhao, Jian Fan, Liqiang
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Molecular Catalysis B: Enzymatic 2011
- 关键词：Cloning - Chromatography - Recombinant proteins - Purification - Organic solvents
- Expression and Characterization of a Novel Enantioselective Lipase from Aspergillus fumigatus被引量：34收藏
- 作者：Shangguan, Jiao-Jiao Fan, Li-Qiang Ju, Xin Zhu, Qing-Qing Wang, Fu-Jun Zhao, Jian Xu, Jian-He
- 机构： State Key Laboratory of Bioreactor Engineering; Institute of Chinese Materia Medica
- 来源：Applied Biochemistry and Biotechnology 2012
- 关键词：Aspergillus - Cloning - Fatty acids - Purification - Escherichia coli - Enantioselectivity
- Efficient Immobilization of Bacterial GH Family 46 Chitosanase by Carbohydrate-Binding Module Fusion for the Controllable Preparation of Chitooligosaccharides被引量：33收藏
- 作者：Lin, Si Qin, Zhen Chen, Qiming Fan, Liqiang Zhou, Jiachun Zhao, Liming
- 机构：East China Univ Sci & Technol;SCICBT
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2019
- 关键词：chitosanase carbohydrate binding module chitooligosaccharide one-step immobilization-purification packed-bed reactor
- The involvement of oxidative stress, neuronal lesions, neurotransmission impairment, and neuroinflammation in acrylamide-induced neurotoxicity in C57/BL6 mice被引量：33收藏
- 作者：Zhao, Mengyao Deng, Linlin Lu, Xiaoxuan Fan, Liqiang Zhu, Yang Zhao, Liming
- 机构：East China Univ Sci & Technol;Wageningen Univ & Res
- 来源：ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 2022
- 关键词：acrylamide Oxidative stress Neurotoxicity Neurotransmission impairment Neuroinflammation NLRP3 inflammasome
- Significantly improved expression and biochemical properties of recombinant serratia marcescens lipase as robust biocatalyst for kinetic resolution of chiral ester被引量：33收藏
- 作者：Wang, Yi Zhao, Jian Xu, Jian-He Fan, Li-Qiang Li, Su-Xia Zhao, Li-Li Mao, Xiao-Bo
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied Biochemistry and Biotechnology 2010
- 关键词：Esters - Gene expression - Recombinant proteins - Escherichia coli - Fatty acids - Affinity chromatography - Lipases - Substrates - Cloning - Metal ions - Metals - Nonionic surfactants - Purification
- 人铜锌超氧化物歧化酶cDNA的克隆、测序及表达被引量：32收藏
- 作者：施惠娟 范立强 魏东芝 袁勤生 孙建新 吴祥甫
- 机构：华东理工大学生物反应器工程国家重点实验室;中国科学院上海生物化学研究所
- 来源：《生物化学与生物物理学报》 1999
- 关键词：表达载体 基因克隆 基因表达 Cu,Zn-SOD
- 摘要：用逆转录聚合酶链反应（ＲＴＰＣＲ），以人胎肝组织总ＲＮＡ为模板，扩增了人铜锌超氧化物歧化酶（ｈＣｕ，ＺｎＳＯＤ）的ｃＤＮＡ，并进行序列分析，将该ｈＣｕ，ＺｎＳＯＤｃＤＮＡ重组到Ｔ７启动子控制下的分泌型表达载体ｐＥＴ２２ｂ...
- 下载全文在线阅读
- 虫草及其发酵制品抗氧化能力研究被引量：32收藏
- 作者：顾宇翔 宋聿文 范立强 袁勤生
- 机构：华东理工大学生物反应器国家重点实验室
- 来源：《中国中药杂志》 2007
- 关键词：抗氧化 冬虫夏草 蛹虫草 发酵
- 摘要：目的:考察天然冬虫夏草、蛹虫草及其发酵制品水和乙醇提取物体外抗氧化活性。方法:测定样品的6个抗氧化指标:抑制亚油酸氧化的能力;清除DPPH自由基、羟自由基、过氧化氢和超氧阴离子的活性以及螯合金属离子(Fe^(2+))的效...
- 下载全文在线阅读
- Expression and Characterization of a Novel Lipase from Aspergillus fumigatus with High Specific activity被引量：31收藏
- 作者：Shangguan, Jiao-Jiao Liu, Yu-Qiang Wang, Fu-Jun Zhao, Jian Fan, Li-Qiang Li, Su-Xia Xu, Jian-He
- 机构：E China Univ Sci & Technol;Shanghai Univ Chinese Tradit Med
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2011
- 关键词：Aspergillus fumigatus Recombinant lipase Expression Characterization active site
- Increasing thermal stability of glutamate decarboxylase from Escherichia. coli by site-directed saturation mutagenesis and its application in GABA production被引量：30收藏
- 作者：Fan, Li-Qiang Li, Ming-Wei Qiu, Yong-jun Chen, Qi-ming Jiang, Si-Jing Shang, Yu-Jie Zhao, Li-Ming
- 机构：East China Univ Sci & Technol;SCICBT
- 来源：JOURNAL OF BIOTECHNOLOGY 2018
- 关键词：Glutamate decarboxylase gamma-Aminobutyrate Site-directed saturation mutagenesis Thermostability Hydrogen bonds
- Heterologous overexpression of Vigna radiata epoxide hydrolase in Escherichia coli and its catalytic performance in enantioconvergent hydrolysis of p-nitrostyrene oxide into (R)-p-nitrophenyl glycol被引量：29收藏
- 作者：Zhu, Qing-Qing He, Wan-Hong Kong, Xu-Dong Fan, Li-Qiang Zhao, Jian Li, Su-Xia Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2014
- 关键词：RT-PCR Vigna radiata epoxide hydrolase p-Nitrostyrene oxide Enantioconvergency Characterization
- Chitobiose alleviates oleic acid-induced lipid accumulation by decreasing fatty acid uptake and triglyceride synthesis in HepG2 cells被引量：29收藏
- 作者：Li, Xiaodan Zhao, Mengyao Fan, Liqiang Cao, Xuni Chen, Liehuan Chen, Junhui Lo, Y. Martin Zhao, Liming
- 机构：East China Univ Sci & Technol;Fuzhou Univ;Huizhou Long Dragon Biotechnol Co Ltd;Peking Univ
- 来源：JOURNAL OF FUNCTIONAL FOODS 2018
- 关键词：Chitobiose Hepatic steatosis Oleic acid CD36 DGAT2
- Bioconversion of chitin into chitin oligosaccharides using a novel chitinase with high chitin-binding capacity被引量：26收藏
- 作者：Zhao, Qiong Fan, Liqiang Deng, Chen Ma, Chunyu Zhang, Chunyue Zhao, Liming
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol SCI;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2023
- 关键词：Chitinase Chitin oligosaccharides Purification Molecular simulation Biodegradation
- Purification of DP 6 to 8 chitooligosaccharides by nanofiltration from the prepared chitooligosaccharides syrup被引量：25收藏
- 作者：Dong, Huizhong Wang, Yaosong Zhao, Liming Zhou, Jiachun Xia, Quanming Jiang, Lihua Fan, Liqiang
- 机构： State Key Laboratory of Bioreactor Engineering; Shanghai Collaborative Innovation Center for Biomanufacturing Technology
- 来源：Bioresources and Bioprocessing 2014
- 关键词：Desorption - Hydrogen bonds - Mass spectrometry - Polymerization - Purification
- Chitooligosaccharide plays essential roles in regulating proline metabolism and cold stress tolerance in rice seedlings被引量：22收藏
- 作者：Zhang, Yang Fan, Liqiang Zhao, Mengyao Chen, Qiming Qin, Zhen Feng, Zhihang Fujiwara, Toru Zhao, Liming
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol SC;Univ Tokyo
- 来源：ACTA PHYSIOLOGIAE PLANTARUM 2019
- 关键词：Chitooligosaccharide Rice seedlings Cold stress Glutamate synthesis Proline metabolism
- Improvement of Ethanol Productivity and Energy Efficiency by Degradation of Inhibitors Using Recombinant Zymomonas mobilis (pHW20a-fdh)被引量：21收藏
- 作者：Dong, Hong-Wei Fan, Li-Qiang Luo, Zichen Zhong, Jian-Jiang Ryu, Dewey D. Y. Bao, Jie
- 机构：E China Univ Sci & Technol;Shanghai Res Inst Chem Ind;Shanghai Jiao Tong Univ;Univ Calif Davis
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2013
- 关键词：recombinant Z mobilis ZM4 (pHW20a-fdh) formate dehydrogenase (fdh) NADH regeneration growth inhibitor inhibitor degradation corn stover hydrolysate (CSH)
- 重组人锰超氧化物歧化酶工程菌高效表达培养工艺的研究被引量：21收藏
- 作者：张艳红 廖晓全 范立强 袁勤生
- 机构：华东理工大学生物工程学院国家重点实验室
- 来源：《中国药学杂志》 2002
- 关键词：重组人锰超氧化物歧化酶 工程菌 培养工艺 rhMn-SOD
- 摘要：目的 确定重组人锰超氧化物歧化酶 (rhMn SOD)工程菌高效表达的最适培养工艺条件。方法 通过摇瓶培养研究了包括接种量、诱导时机、Mn2 + 浓度、诱导后培养时间、发酵过程中 pH变化以及初始 pH值对发酵的影响。 ...
- 下载全文在线阅读
- Chemoenzymatic enantioconvergent hydrolysis of p-nitrostyrene oxide into (R)-p-nitrophenyl glycol by a newly cloned epoxide hydrolase VrEH2 from Vigna radiata被引量：21收藏
- 作者：Wu, Yan-Wen Kong, Xu-Dong Zhu, Qing-Qing Fan, Li-Qiang Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：CATALYSIS COMMUNICATIONS 2015
- 关键词：Vigna radiata epoxide hydrolase Recombinant expression Enzymatic property Enantioconvergency Chemoenzymatic synthesis (R)-p-nitrophenyl glycol
- A novel human derived cell-penetrating peptide in drug delivery被引量：20收藏
- 作者：Zhao, Jian Gao, Peng Xiao, Wei Fan, Li-Qiang Wang, Fu-Jun Li, Su-Xia Liu, Jian-Wen
- 机构：E China Univ Sci & Technol;Zhejiang Reachall Pharmaceut Co Ltd
- 来源：MOLECULAR BIOLOGY REPORTS 2011
- 关键词：hC-SOD3 Human cell-penetrating peptide Apoptin Drug delivery
- Chitooligosaccharides enhance cold tolerance by repairing photodamaged PS II in rice被引量：19收藏
- 作者：Zhou, Jiachun Chen, Qiao Zhang, Yang Fan, Liqiang Qin, Zhen Chen, Qiming Qiu, Yongjun Jiang, Lihua Zhao, Liming
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol SC
- 来源：JOURNAL OF AGRICULTURAL SCIENCE 2018
- 关键词：Chitooligosaccharides cold tolerance PS II repairing rice stress-responsive gene
- Gene cloning and expression of a novel hypoglycaemic peptide from Momordica charantia被引量：18收藏
- 作者：Wang, Bi-lian Zhang, Wen-ji Zhao, Jian Wang, Fu-jun Fan, Li-qiang Wu, Yi-xin Hu, Zhi-bi
- 机构：E China Univ Sci & Technol;Shanghai Univ Chinese Tradit Med;Hamamatsu Univ Sch Med
- 来源：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE 2011
- 关键词：Momordica charantia gene cloning polypeptide-P hypoglycaemic effect
- Structure-function analysis of Gynuella sunshinyii chitosanase uncovers the mechanism of substrate binding in GH family 46 members被引量：18收藏
- 作者：Wang, Yani Qin, Zhen Fan, Liqiang Zhao, Liming
- 机构：East China Univ Sci & Technol;Shanghai Univ;Shanghai Collaborat Innovat Ctr Biomfg Technol SC
- 来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2020
- 关键词：Chitooligosaccharides Chitosanase Complex structure Substrate recognition GH family 46
- De novo biosynthesis of 2′-fucosyllactose in engineered Pichia pastoris被引量：18收藏
- 作者：Qian, Difan Zhang, Chunyue Deng, Chen Zhou, Mian Fan, Liqiang Zhao, Liming
- 机构：East China Univ Sci & Technol;Shanghai Changzheng Hosp;Shanghai Collaborat Innovat Ctr Biomfg Technol SCI
- 来源：BIOTECHNOLOGY LETTERS 2023
- 关键词：Biosynthesis 2 '-Fucosyllactose Human milk oligosaccharides Pichia pastoris
