成果/Result
- Metabolic engineering coupled with adaptive evolution strategies for the efficient production of high-quality L-lactic acid by Lactobacillus paracasEI被引量：65收藏
- 作者：Tian, Xiwei Liu, Xuehua Zhang, Yifan Chen, Yang Hang, Haifeng Chu, Ju Zhuang, Yingping
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCE TECHNOLOGY 2021
- 关键词：Lactobacillus paracasEI Lactic acid CRISPR Cas9 adaptive evolution Open fermentation
- Xanthan-Curdlan nexus for synthesizing edible food packaging films被引量：61收藏
- 作者：Mohsin, Ali Zaman, Waqas Qamar Guo, Meijin Ahmed, Waheed Khan, Imran Mahmood Niazi, Sobia Rehman, Abdur Hang, Haifeng Zhuang, Yingping
- 机构：East China Univ Sci & Technol;Natl Univ Sci & Technol NUST;Jiangnan Univ
- 来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2020
- 关键词：Xanthangum Curdlan Blendfilms
- Sustainable biosynthesis of curdlan from orange waste by using Alcaligenes faecalis: A systematically modeled approach被引量：48收藏
- 作者：Mohsin, Ali Sun, Jingyun Khan, Imran Mahmood Hang, Haifeng Tariq, Muhammad Tian, Xiwei Ahmed, Waqas Niazi, Sobia Zhuang, Yingping Chu, Ju Mohsin, Muhammad Zubair Salim-ur-Rehman Guo, Meijin
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Jiangnan Univ;Univ Agr Faisalabad;Univ Agr Faisalabad
- 来源：CARBOHYDRATE POLYMERS 2019
- 关键词：Sustainable biosynthesis Orange peels Kinetics Curdlan Batch culture fermentation
- Kinetic analysis of curdlan production by Alcaligenes faecalis with maltose, sucrose, glucose and fructose as carbon sources被引量：42收藏
- 作者：Zhang, Qin Sun, Jingyun Wang, Zejian Hang, Haifeng Zhao, Wei Zhuang, Yingping Chu, Ju
- 机构： State Key Laboratory of Bioreactor Engineering; Shan Dong Fu Yang Biological Technology Co.
- 来源：Bioresource Technology 2018
- 关键词：Carbon - Rate constants - Fructose - Sugar (sucrose) - Biochemistry - Glucose - Growth kinetics - Fermentation - Maltose - Cell growth
- Enhancement of erythromycin A production with feeding available nitrogen sources in erythromycin biosynthesis phase被引量：36收藏
- 作者：Zou, Xiang Hang, Hai-feng Chu, Ju Zhuang, Ying-ping Zhang, Si-liang
- 机构：E China Univ Sci & Technol;Southwest Univ
- 来源：BIORESOURCE TECHNOLOGY 2009
- 关键词：Available nitrogen sources Biosynthesis phase Erythromycin A Industrial scale Scale up
- 人工神经网络在红霉素发酵过程状态预估中的应用被引量：34收藏
- 作者：黄明志 杭海峰 储炬 叶勤 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2000
- 关键词：神经网络 BP网络 RBF网络 红霉素 发酵 状态预估
- 摘要：探索了动态 BP网络和 RBF网络在红霉素发酵过程状态预估中的应用 ,比较了它们的收敛速度和学习能力。结果表明 ,BP网络和 RBF网络都具有相当好的学习能力 ,但 RBF网络的收敛速度更快。训练好的神经网络 ,在红霉素...
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- Effect of glycosylation on biochemical characterization of recombinant phytase expressed in Pichia pastoris被引量：25收藏
- 作者：Guo, Meijin Hang, Haifeng Zhu, Taicheng Zhuang, Yingping Chu, Ju Zhang, Siliang
- 机构： State Key Laboratory of Bioreactor Engineering; Department of Biotechnology
- 来源：Enzyme and Microbial Technology 2008
- 关键词：Amino acids - Biochemical engineering - Glycosylation - pH - Thermodynamic stability
- 培养条件对重组毕赤酵母高密度表达猪胰岛素前体的影响被引量：23收藏
- 作者：姚钰舜 储炬 杭海峰 庄英萍 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2006
- 关键词：毕赤酵母 猪胰岛素前体 高密度培养 高表达
- 摘要：研究了基因工程菌P ich ia p astotis高密度、高表达的培养条件。在全合成摇瓶培养基的基础上,考察了诱导过程中甲醇浓度、温度、pH、(NH4)2SO4及磷酸盐浓度等因素对猪胰岛素前体(P IP)表达的影响。研...
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- Transcriptional investigation of the effect of mixed feeding to identify the main cellular stresses on recombinant Pichia pastoris被引量：21收藏
- 作者：Zhu, Taicheng Hang, Haifeng Chu, Ju Zhuang, Yingping Zhang, Siliang Guo, Meijin
- 机构： Institute of Microbiology; State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Industrial Microbiology and Biotechnology 2013
- 关键词：Genes - Insulin - Metabolism - Methanol - Physiology - Recombinant protEIns - Yeast
- A simple fermentation strategy for high-level production of recombinant phytase by Pichia pastoris using glucose as the growth substrate被引量：18收藏
- 作者：Hang, Haifeng Ye, Xinhao Guo, Meijin Chu, Ju Zhuang, Yingping Zhang, Ming Zhang, Siliang
- 机构： State Key Laboratory of Bioreactor Engineering; Department of Biotechnology
- 来源：Enzyme and Microbial Technology 2009
- 关键词：Substrates - Yeast - Fermentation - Methanol - Recombinant protEIns - Glucose oxidase - Glucose sensors
- Kinetic analysis of sodium gluconate production by Aspergillus niger with different inlet oxygen concentrations被引量：18收藏
- 作者：Tian, Xiwei Shen, Yuting Zhuang, Yingping Zhao, Wei Hang, Haifeng Chu, Ju
- 机构：East China Univ Sci & Technol;Shan Dong Fuyang Biol Technol Co Ltd
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2018
- 关键词：Aspergillus niger Sodium gluconate Oxygen Kinetics
- Target-site directed rational high-throughput screening system for high sophorolipids production by Candida bombicola被引量：17收藏
- 作者：Chen, Yang Tian, Xiwei Li, Qianhui Li, Ya Chu, Ju Hang, Haifeng Zhuang, Yingping
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCE TECHNOLOGY 2020
- 关键词：Candida bombicola Sophorolipids Combined mutagenesis Relaxation culture Combined pressure
- Application of oxygen uptake rate and response surface methodology for erythromycin production by Saccharopolyspora erythraea被引量：15收藏
- 作者：Zou, Xiang Hang, Hai-Feng Chen, Chang-Fa Chu, Ju Zhuang, Ying-Ping Zhang, Si-Liang
- 机构： State Key Laboratory of Bioreactor Engineering; School of Pharmaceutical Sciences
- 来源：Journal of Industrial Microbiology and Biotechnology 2008
- 关键词：Amino acids - Antibiotics - Batch cell culture - Biochemistry - Bioreactors - Citric acid - Cultivation - Nutrients - Oxygen - Physiological models - Sulfur compounds - Surface properties
- Oxygen uptake rate optimization with nitrogen regulation for erythromycin production and scale-up from 50 L to 372 m3 scale被引量：14收藏
- 作者：Zou, Xiang Hang, Hai-feng Chu, Ju Zhuang, Ying-ping Zhang, Si-liang
- 机构： State Key Laboratory of Bioreactor Engineering; School of Pharmaceutical Sciences
- 来源：Bioresource Technology 2009
- 关键词：Biochemistry - Metabolism - Biosynthesis - Fermenters - Lactic acid - Enzyme activity - Fermentation - Nitrogen - Antibiotics - Oxygen - Thermodynamic stability - Physiology - Propionic acid
- UnvEIling the mechanism of efficient β-phenylethyl alcohol conversion in wild-type Saccharomyces cerevisiae WY319 through multi-omics analysis被引量：13收藏
- 作者：Yang, Chenghan Ren, Yilin Ge, Lihao Xu, Wenting Hang, Haifeng Mohsin, Ali Tian, Xiwei Chu, Ju Zhuang, Yingping
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY JOURNAL 2024
- 关键词：high-yield mechanism high-yielding strain multi-omics respiratory metabolism Saccharomyces cerevisiae beta-phenylethanol
- Co-culture with TM4 cells enhances the proliferation and migration of rat adipose-derived mesenchymal stem cells with high stemness被引量：12收藏
- 作者：Luo, Yanxia Mohsin, Ali Xu, Chenze Wang, Qizheng Hang, Haifeng Zhuang, Yingping Chu, Ju Guo, Meijin
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：CYTOTECHNOLOGY 2018
- 关键词：adipose-derived mesenchymal stem cells TM4 cells Co-culture mode Cell proliferation and migration Stemness
- Polyphenol oxidase inhibition by Saccharomyces cerevisiae extracts: A promising approach to prevent enzymatic browning被引量：12收藏
- 作者：Liu, Zebo Ding, Hui Martuscelli, Maria Sajid, Saria Hang, Haifeng Mohsin, Ali Zhu, Xiaofeng Sun, Huijie Xia, Jianye Yin, Zhongping Zhuang, Yingping Guo, Meijin Wang, Zejian
- 机构：East China Univ Sci & Technol;Univ Teramo;Riphah Int Univ;Jiangxi Agr Univ;East China Univ Sci & Technol
- 来源：LWT-FOOD SCIENCE AND TECHNOLOGY 2024
- 关键词：Polyphenol oxidase inhibitor Saccharomyces cerevisiae extracts Reduced glutathione Anti-browning Molecular docking and dynamics simulation
- Oxygen-enriched fermentation of sodium gluconate by Aspergillus niger and its impact on intracellular metabolic flux distributions被引量：10收藏
- 作者：Shen, Yuting Tian, Xiwei Zhao, Wei Hang, Haifeng Chu, Ju
- 机构：East China Univ Sci & Technol;Shan Dong Fuyang Biol Technol Co Ltd
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2018
- 关键词：Gluconic acid Aspergillus niger Oxygen Metabolic flux distribution
- Promoter screening and identification for metabolic regulation in Acremonium chrysogenum被引量：10收藏
- 作者：Liu, Ling Li, Runge Zhang, Xinrun Chen, Zhen Mohsin, Ali Hang, Haifeng Tian, Xiwei Chu, Ju
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;POB 329
- 来源：BIOTECHNOLOGY JOURNAL 2024
- 关键词：Acremonium chrysogenum bidirectional promoter cephalosporin C Golden Gate assembly promoter library
- Combined available nitrogen resources enhanced erythromycin production and preliminary exploration of metabolic flux analysis under nitrogen perturbations被引量：9收藏
- 作者：Zhang, Qi Hang, Haifeng Tian, Xiwei Zeng, Wei Yu, Zhenhua Wang, Xiaojian Tang, Yin Zhuang, Yingping Chu, Ju
- 机构：East China Univ Sci & Technol;Yidu HEC Biochem Co Ltd
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2019
- 关键词：Erythromycin Available nitrogen sources Corn steep liquor Yeast powder Metabolic flux
- Application of 8-parallel micro-bioreactor system with non-invasive optical pH and DO biosensor in high-throughput screening of L-lactic acid producing strain被引量：8收藏
- 作者：Tian, Xiwei Zhou, Gang Wang, Weifei Zhang, Ming Hang, Haifeng Mohsin, Ali Chu, Ju Zhuang, Yingping Zhang, Siliang
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2018
- 关键词：High-throughput screening Lactic acid Lactobacillus paracasEI Micro-bioreactor Optical biosensor
- The "Coexistence" of Ehrlich pathway and de novo pathway improves 2-PE synthesis in Saccharomyces cerevisiae被引量：6收藏
- 作者：Yang, Chenghan Lv, Qingqing Zhang, Li Jia, Huining Chen, Hailong Yang, Sainan Qiu, Yuxin Hang, Haifeng Mohsin, Ali Chu, Ju Zhuang, Yingping Tian, Xiwei
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：CHEMICAL ENGINEERING JOURNAL 2025
- 关键词：beta-Phenylethanol Saccharomyces cerevisiae Coexisting metabolism Specific growth rate Chemostat culture
- Influence of initial glucose concentration on seed culture of sodium gluconate production by Aspergillus niger被引量：6收藏
- 作者：Liu, Xiu Tian, Xiwei Hang, Haifeng Zhao, Wei Wang, Yonghong Chu, Ju
- 机构：East China Univ Sci & Technol;Shan Dong Fuyang Biol Technol Co Ltd
- 来源：BIORESOURCES AND BIOPROCESSING 2017
- 关键词：Aspergillus niger Sodium gluconate Initial glucose concentration Osmotic pressure Seed culture Metabolite profiling
- 重组Pichia酵母(Mut^S)发酵过渡阶段关键酶活分析被引量：6收藏
- 作者：胡光星 郭美锦 储炬 杭海峰 庄英萍 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室国家生化工程技术研究中心
- 来源：《华东理工大学学报（自然科学版）》 2004
- 关键词：重组巴氏毕赤酵母 代谢途径 过渡阶段 植酸酶
- 摘要：重组Pichia酵母表达系统的发酵存在从利用甘油为碳源生长到利用甲醇为碳源表达外源蛋白的发酵表达过渡阶段。Pichia酵母过渡阶段碳源代谢途径关键酶酶活分析表明:甲醇诱导3hAOX2酶活为0,4h时突然增加到0.05U,...
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- Response Surface Methodology for Optimization of the Erythromycin Production by Fed-Batch Fermentation Using an Inexpensive Biological Nitrogen Source被引量：5收藏
- 作者：Zou, X. Chen, C. -F. Hang, H. -F. Chu, J. Zhuang, Y. -P. Zhang, S. -L.
- 机构：E China Univ Sci & Technol;Southwest Univ
- 来源：CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY 2010
- 关键词：Biological nitrogen erythromycin production fed-batch strategy medium optimization response surface methodology Saccharopolyspora etythraea
- Variable selection in partial least squares with the wEIghted variable contribution to the first singular value of the covariance matrix被引量：5收藏
- 作者：Lin, Weilu Hang, Haifeng Zhuang, Yingping Zhang, Siliang
- 机构：East China Univ Sci & Technol
- 来源：CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS 2018
- 关键词：Informative variables Interval variable selection Partial least squares Variable contribution Maximal singular value Spectroscopy
- 红外光谱-人工神经元网络法测定海藻培养过程中的碳酸盐浓度被引量：3收藏
- 作者：杭海峰 储炬 叶勤 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2005
- 关键词：红外光谱 人工神经元网络 海藻培养 碳酸盐
- 摘要：用傅里叶变换红外光谱法测定海藻培养液中碳酸盐浓度时,由于pH对碳酸盐特征吸收峰影响严重,本文用人工神经元网络处理红外光谱,用该方法计算得到对标准样品的预测标准误差为NaHCO30.08g/L,pH值为0.12,优于偏最小...
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- Numerical and experimental assessment of a miniature bioreactor equipped with a mechanical agitator and non-invasive biosensors被引量：3收藏
- 作者：Li, Chao Tian, Jiangtao Wang, Weifei Peng, Huadong Zhang, Ming Hang, Haifeng Zhang, Siliang Xia, Jian-Ye
- 机构：East China Univ Sci & Technol;Imperial Coll London;Imperial Coll London
- 来源：JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 2019
- 关键词：miniature bioreactor computational fluid dynamics mass transfer shear rate mixing
- Alterations in ProtEIn Phosphorylation and Arginine Biosynthesis Metabolism Confer β-Phenylethanol Tolerance in Saccharomyces cerevisiae被引量：1收藏
- 作者：Yang, Chenghan Ren, Yilin Zhang, Li Li, Yina Wang, Chunxia Hang, Haifeng Tian, Xiwei Mohsin, Ali Chu, Ju Zhuang, Yingping
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2025
- 关键词：adaptive laboratory evolution arginine phosphoproteome
Saccharomyces cerevisiae beta-phenylethanol
- Systematic engineering of Micromonospora echinospora cell factory for gentamicin C1a overproduction被引量：1收藏
- 作者：Xu, Feng Gao, Hao Ben, Rong Hu, Kaihao Wang, Yuan Mohsin, Ali Guo, Yuanxin Li, Xu Hang, Haifeng Chu, Ju Tian, Xiwei
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：TRENDS IN BIOTECHNOLOGY 2026
- 关键词：Antibiotics - Biochemistry - Biosynthesis - Genes - Metabolism - Optimization
