成果/Result
- Biocatalytic ketone reduction: A green and efficient access to enantiopure alcohols被引量：200收藏
- 作者：Ni, Yan Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY ADVANCES 2012
- 关键词：Asymmetric reduction Biocatalysis Chiral alcohols Genome mining Reductases
- Cloning and characterization of a panel of constitutive promoters for applications in pathway engineering in Saccharomyces cerevisiae被引量：149收藏
- 作者：Sun, Jie Shao, Zengyi Zhao, Hua Nair, Nikhil Wen, Fei Xu, Jian-He Zhao, Huimin
- 机构：Univ Illinois;E China Univ Sci & Technol;Univ Illinois
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2012
- 关键词：yeast promoters xylanases zeaxanthin consolidated bioprocessing synthetic biology
- New opportunities for biocatalysis: driving the synthesis of chiral chemicals被引量：148收藏
- 作者：Zheng, Gao-Wei Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：CURRENT OPINION IN BIOTECHNOLOGY 2011
- 关键词：Stereochemistry - Biocatalysts - Biochemical engineering
- Enhancing effect of Tween-80 on lipase performance in enantioselective hydrolysis of ketoprofen ester被引量：113收藏
- 作者：Liu, YY Xu, JH Hu, Y
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2000
- 关键词：Candida rugosa lipase enantioselectivity activity ketoprofen Tween-80
- Asymmetric Amination of Secondary Alcohols by using a Redox-Neutral Two-Enzyme Cascade被引量：112收藏
- 作者：Chen, Fei-Fei Liu, You-Yan Zheng, Gao-Wei Xu, Jian-He
- 机构：E China Univ Sci & Technol;Guangxi Univ;Guangxi Acad Sci
- 来源：CHEMCATCHEM 2015
- 关键词：amine dehydrogenase asymmetric catalysis biotransformations cascade reaction chiral amines
- Reshaping the Active Pocket of Amine Dehydrogenases for Asymmetric Synthesis of Bulky Aliphatic Amines被引量：102收藏
- 作者：Chen, Fei-Fei Zheng, Gao-Wei Liu, Lei Li, Hao Chen, Qi Li, Fu-Long Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol
- 来源：ACS CATALYSIS 2018
- 关键词：amine dehydrogenase reductive amination chiral amine protEIn engineering biocatalysis substrate scope
- Development of an Engineered Ketoreductase with Simultaneously Improved Thermostability and Activity for Making a Bulky Atorvastatin Precursor被引量：101收藏
- 作者：Gong, Xu-Min Qin, Zhen Li, Fu-Long Zeng, Bu-Bing Zheng, Gao-Wei Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：ACS CATALYSIS 2019
- 关键词：biocatalysis asymmetric reduction ketoreductase protEIn engineering thermostabilization atorvastatin precursor
- Reshaping an Enzyme Binding Pocket for Enhanced and Inverted Stereoselectivity: Use of Smallest Amino Acid Alphabets in Directed Evolution被引量：98收藏
- 作者：Sun, Zhoutong Lonsdale, Richard Kong, Xu-Dong Xu, Jian-He Zhou, Jiahai Reetz, Manfred T.
- 机构：Max Planck Inst Kohlenforsch;Univ Marburg;E China Univ Sci & Technol;Chinese Acad Sci
- 来源：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2015
- 关键词：amino acid alphabet directed evolution epoxide hydrolases saturation mutagenesis stereoselectivity
- Whole cell microbial transformation in cloud point system被引量：84收藏
- 作者：Wang, Zhilong Xu, Jian-He Chen, Daijie
- 机构： School of Pharmacy; Laboratory of Biocatalysis and Bioprocessing; Shanghai Institutes of Pharmacy Industry
- 来源：Journal of Industrial Microbiology and Biotechnology 2008
- 关键词：Biocompatibility - Nonionic surfactants - Phase separation - Solvent extraction
- Bioproduction of Glycolic Acid from Glycolonitrile with a New Bacterial Isolate of Alcaligenes sp ECU0401被引量：83收藏
- 作者：He, Yu-Cai Xu, Jian-He Su, Jin-Huan Zhou, Li
- 机构：E China Univ Sci & Technol;Chinese Acad Sci
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2010
- 关键词：Nitrilase Alcaligenes sp ECU0401 Glycolic acid Glycolonitrile Immobilization
- Preparation of Structurally Diverse Chiral Alcohols by Engineering Ketoreductase CgKR1被引量：82收藏
- 作者：Zheng, Gao-Wei Liu, Yuan-Yang Chen, Qi Huang, Lei Yu, Hui-Lei Lou, Wen-Yong Li, Chun-Xiu Bai, Yun-Peng Li, Ai-Tao Xu, Jian-He
- 机构：East China Univ Sci & Technol;South China Univ Technol;Max Planck Inst Kohlenforsch
- 来源：ACS CATALYSIS 2017
- 关键词：asymmetric reduction ketoreductase chiral alcohol protEIn engineering substrate specificity biocatalysis
- Newly Identified Thermostable Esterase from Sulfobacillus acidophilus: Properties and Performance in Phthalate Ester Degradation被引量：77收藏
- 作者：Zhang, Xiao-Yan Fan, Xiang Qiu, Yong-Jun Li, Cheng-Yuan Xing, Shuai Zheng, Yi-Tao Xu, Jian-He
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2014
- 关键词：Escherichia coli - Esters - Hydrolysis
- A Motif-Based Deep Learning Tool for the Identification of Unusual NADH-Dependent Imine Reductases被引量：73收藏
- 作者：Shen, Xin-Yuan Wu, Yu-Xuan Ma, Zhi-Feng Yi, Xiao Shi, Min Zhu, Zhen-Yu Jin, Tian Hu, Xiao-Yu Huang, Zi-Yi Gao, Yun-Fei Chen, Qi Yu, Hui-Qun Xu, Jian-He Fan, Gui-Sheng Zheng, Gao-Wei
- 机构： State Key Laboratory of Bioreactor Engineering; School of Information Science and Engineering
- 来源：SSRN 2025
- 关键词：Amination - Enzyme immobilization - Enzyme inhibition - Enzyme kinetics - Hydrolases - Lipases
- Whole-Cell-Catalyzed Multiple Regio- and Stereoselective Functionalizations in Cascade Reactions Enabled by Directed Evolution被引量：72收藏
- 作者：Li, Aitao Ilie, Adriana Sun, Zhoutong Lonsdale, Richard Xu, Jian-He Reetz, Manfred T.
- 机构：Max Planck Inst Kohlenforsch;Univ Marburg;East China Univ Sci & Technol
- 来源：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2016
- 关键词：cascade reactions C-H activation directed evolution enantioselectivity P450 monooxygenase
- Biocatalytic properties of a recombinant aldo-keto reductase with broad substrate spectrum and excellent stereoselectivity被引量：68收藏
- 作者：Ni, Yan Li, Chun-Xiu Ma, Hong-Min Zhang, Jie Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2011
- 关键词：YtbE Recombinant aldo-keto reductase Bacillus sp. Asymmetric reduction Chiral alcohols
- Enantioselective Synthesis of Chiral Vicinal Amino Alcohols Using Amine Dehydrogenases被引量：67收藏
- 作者：Chen, Fei-Fei Cosgrove, Sebastian C. Birmingham, William R. Mangas-Sanchez, Juan Citoler, Joan Thompson, Matthew P. Zheng, Gao-Wei Xu, Jian-He Turner, Nicholas J.
- 机构：East China Univ Sci & Technol;Univ Manchester;EnginZyme AB
- 来源：ACS CATALYSIS 2019
- 关键词：amine dehydrogenase biocatalysis chiral amino alcohols directed evolution reductive amination
- Two-step enzymatic synthesis of ursodeoxycholic acid with a new 7β-hydroxysteroid dehydrogenase from Ruminococcus torques被引量：66收藏
- 作者：Zheng, Ming-Min Wang, Ru-Feng Li, Chun-Xiu Xu, Jian-He
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol;China Pharmaceut Univ
- 来源：PROCESS BIOCHEMISTRY 2015
- 关键词：Ursodeoxycholic acid Chenodeoxycholic acid 7 alpha-Hydroxysteroid dehydrogenase 7 beta-Hydroxysteroid dehydrogenase Two-step reaction
- Chemical modulators for enzymatic reactions被引量：66收藏
- 作者：Xu, Jian-He Zheng, Gao-Wei Luo, Xiao-Jing
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology
- 来源：RSC Green Chemistry 2015
- Switching Cofactor Dependence of 7β-Hydroxysteroid Dehydrogenase for Cost-Effective Production of Ursodeoxycholic Acid被引量：66收藏
- 作者：You, Zhi-Neng Chen, Qi Shi, Shou-Cheng Zheng, Ming-Min Pan, Jiang Qian, Xiao-Long Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Suzhou Bioforany EnzyTech Co Ltd
- 来源：ACS CATALYSIS 2019
- 关键词：biocatalysis cofactor dependence small-and-smart library 7 beta-hydroxysteroid dehydrogenase molecular dynamics simulation ursodeoxycholic acid
- Bio-resolution of a chiral epoxide using whole cells of Bacillus megaterium ECU1001 in a biphasic system被引量：65收藏
- 作者：Gong, Peng-Fei Xu, Jian-He
- 机构： Lab. Biocatalysis and Bioprocessing
- 来源：Enzyme and Microbial Technology 2005
- 关键词：Biocatalysts - Cells - Concentration (process) - Ethers - Optimization - Solubility
- Engineering of Cyclohexanone Monooxygenase for the Enantioselective Synthesis of (S)-Omeprazole被引量：59收藏
- 作者：Zhang, Yan Wu, Yin-Qi Xu, Na Zhao, Qian Yu, Hui-Lei Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Jiangsu Aosaikang Pharmaceut Co Ltd
- 来源：ACS SUSTAINABLE CHEMISTRY & ENGINEERING 2019
- 关键词：Biocatalysis Cyclohexanone monooxygenase Substrate specificity ProtEIn engineering Esomeprazole sulfoxide Halo-based selection method
- Access to optically active aryl halohydrins using a substrate-tolerant carbonyl reductase discovered from kluyveromyces thermotolerans被引量：59收藏
- 作者：Xu, Guo-Chao Yu, Hui-Lei Zhang, Xiao-Yan Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology
- 来源：ACS Catalysis 2012
- 关键词：Data mining - Escherichia coli - Enzyme activity - Genes - Ketones
- Stereoselective synthesis of L-tert-leucine by a newly cloned leucine dehydrogenase from Exiguobacterium sibiricum被引量：58收藏
- 作者：Li, Jing Pan, Jiang Zhang, Jie Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2014
- 关键词：Leucine dehydrogenase Exiguobacterium sibiricum Reductive amination Dual enzyme co-expression L-tert-Leucine
- Engineering 7β-Hydroxysteroid Dehydrogenase for Enhanced Ursodeoxycholic Acid Production by Multiobjective Directed Evolution被引量：57收藏
- 作者：Zheng, Ming-Min Chen, Ke-Cai Wang, Ru-Feng Li, Hao Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2017
- 关键词：biocatalysis cascade reaction 7 beta-hydroxysteroid dehydrogenase multiobjective directed evolution ursodeoxycholic acid
- Direct conversion of xylan to ethanol by recombinant Saccharomyces cerevisiae strains displaying an engineered minihemicellulosome被引量：54收藏
- 作者：Sun, Jie Wen, Fei Si, Tong Xu, Jian-He Zhao, Huimin
- 机构： Department of Chemical and Biomolecular Engineering; State Key Laboratory of Bioreactor Engineering; Departments of Chemistry
- 来源：Applied and Environmental Microbiology 2012
- 关键词：Hydrolysis - Enzymes - Principal component analysis - Yeast - Cell membranes
- Discovery of an Imine Reductase for Reductive Amination of Carbonyl Compounds with Sterically Challenging Amines被引量：54收藏
- 作者：Chen, Fei-Fei He, Xue-Feng Zhu, Xin-Xin Zhang, Zhi Shen, Xin-Yuan Chen, Qi Xu, Jian-He Turner, Nicholas J. Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol;Univ Manchester
- 来源：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2023
- 关键词：Carbonyl compounds - Nucleophiles - Substrates - Synthesis (chemical)
- A thermostable and organic-solvent tolerant esterase from Pseudomonas putida ECU1011: Catalytic properties and performance in kinetic resolution of α-hydroxy acids被引量：49收藏
- 作者：Ma, Bao-Di Yu, Hui-Lei Pan, Jiang Liu, Jia-Yan Ju, Xin Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：BIORESOURCE TECHNOLOGY 2013
- 关键词：Esterase Thermostability Organic-solvent tolerance alpha-Hydroxy (carboxylic) acids Deacetylation
- Catalytic conversion of corncob to furfuryl alcohol in tandem reaction with tin-loaded sulfonated zeolite and NADPH-dependent reductase biocatalyst被引量：48收藏
- 作者：Li, Yuan-Yuan Li, Qing Zhang, Peng-Qi Ma, Cui-Luan Xu, Jian-He He, Yu-Cai
- 机构：Changzhou Univ;Hubei Univ;East China Univ Sci & Technol;Huaiyin Normal Univ
- 来源：BIORESOURCE TECHNOLOGY 2021
- 关键词：Corncob Sn-zeolite catalyst Furfuryl alcohol Reductase Recombinant E. coli CG-19
- Hydroxylases involved in terpenoid biosynthesis: a review被引量：47收藏
- 作者：Zhang, Zihan Wu, Qing-Yang Ge, Yue Huang, Zheng-Yu Hong, Ran Li, Aitao Xu, Jian-He Yu, Hui-Lei
- 机构：East China Univ Sci & Technol;Chinese Acad Sci;Hubei Univ
- 来源：BIORESOURCES AND BIOPROCESSING 2023
- 关键词：Biooxidation Cytochrome P450 monooxygenase Hydroxylase Natural products Terpenoids
- Highly efficient synthesis of ethyl (S)-4-chloro-3-hydroxybutanoate and its derivatives by a robust NADH-dependent reductase from E-coli CCZU-K14被引量：46收藏
- 作者：He, Yu-Cai Tao, Zhi-Cheng Zhang, Xian Yang, Zhen-Xing Xu, Jian-He
- 机构：Changzhou Univ;E China Univ Sci & Technol
- 来源：BIORESOURCE TECHNOLOGY 2014
- 关键词：Asymmetric reduction Biotransformation Ethyl 4-chloro-3-oxobutanoate Ethyl (S)-4-chloro-3-hydroxybutanoate Recombinant E. coli CCZU-K14
