成果/Result
- Facile, high efficiency immobilization of lipase enzyme on magnetic iron oxide nanoparticles via a biomimetic coating被引量：242收藏
- 作者：Ren, Yuhong Rivera, Jose G. He, Lihong Kulkarni, Harsha Lee, Dong-Keun Messersmith, Phillip B.
- 机构：E China Univ Sci & Technol;Northwestern Univ;Northwestern Univ;Northwestern Univ;Northwestern Univ
- 来源：BMC BIOTECHNOLOGY 2011
- 关键词：Nanomagnetics - Cost effectiveness - Biomimetics - Enzyme immobilization - Iron oxides - Metal nanoparticles
- The yeast peroxisome: A dynamic storage depot and subcellular factory for squalene overproduction被引量：194收藏
- 作者：Liu, Guo-Song Li, Tian Zhou, Wei Jiang, Min Tao, Xin-Yi Liu, Min Zhao, Ming Ren, Yu-Hong Gao, Bei Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2020
- 关键词：Synthetic biology Saccharomyces cerevisiae Peroxisome Storage depot Subcellular factory Terpene
- Metabolic compartmentalization in yeast mitochondria: Burden and solution for squalene overproduction被引量：115收藏
- 作者：Zhu, Zhan-Tao Du, Meng-Meng Gao, Bei Tao, Xin-Yi Zhao, Ming Ren, Yu-Hong Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2021
- 关键词：Saccharomyces cerevisiae Mitochondrial engineering Metabolic burden Squalene Dual engineering
- Fe3+-induced oxidation and coordination cross-linking in catechol-chitosan hydrogels under acidic pH conditions被引量：82收藏
- 作者：Guo, Zhongwei Ni, Kefeng Wei, Dongzhi Ren, Yuhong
- 机构：E China Univ Sci & Technol
- 来源：RSC ADVANCES 2015
- 关键词：pH - Self assembly - Phenols - Chitosan - Hydrogels - Gelation
- Metabolic Engineering of Saccharomyces cerevisiae To Overproduce Squalene被引量：71收藏
- 作者：Li, Tian Liu, Guo-Song Zhou, Wei Jiang, Min Ren, Yu-Hong Tao, Xin-Yi Liu, Min Zhao, Ming Wang, Feng-Qing Gao, Bei Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2020
- 关键词：Saccharomyces cerevisiae metabolic engineering squalene overproduction ethanol carbon source utilization
- Artificial Multienzyme Supramolecular Device: Highly Ordered Self-Assembly of Oligomeric Enzymes In Vitro and In Vivo被引量：68收藏
- 作者：Gao, Xin Yang, Shuai Zhao, Chengcheng Ren, Yuhong Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2014
- 关键词：biocatalysis enzyme catalysis oligomerization self-assembly supramolecular chemistry
- Reversion of multidrug resistance by tumor targeted delivery of antisense oligodeoxynucleotides in hydroxypropyl-chitosan nanoparticles被引量：52收藏
- 作者：Wang, Jiaqi Tao, Xinyi Zhang, Yufei Wei, Dongzhi Ren, Yuhong
- 机构：E China Univ Sci & Technol
- 来源：BIOMATERIALS 2010
- 关键词：Hydroxypropyl-chitosan nanoparticles Tumor-targeted Antisense oligodeoxynucleotides Multidrug resistance
- One-step purification and immobilization of his-tagged protEIn via Ni2+-functionalized Fe3O4@polydopamine magnetic nanoparticles被引量：47收藏
- 作者：Yang, Jianbing Ni, Kefeng Wei, Dongzhi Ren, Yuhong
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY AND BIOPROCESS ENGINEERING 2015
- 关键词：His-tagged protEIn immobilization magnetic nanoparticles polydopamine purification
- Enhancement of the activity of enzyme immobilized on polydopamine-coated iron oxide nanoparticles by rational orientation of formate dehydrogenase被引量：44收藏
- 作者：Gao, Xin Ni, Kefeng Zhao, Chengcheng Ren, Yuhong Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2014
- 关键词：Directional immobilization Site-directed mutagenesis Formate dehydrogenase Iron oxide nanoparticles Polydopamine
- Enhancing Biosynthesis of a Ginsenoside Precursor by Self-Assembly of Two Key Enzymes in Pichia pastoris被引量：36收藏
- 作者：Zhao, Chengcheng Gao, Xin Liu, Xinbin Wang, Yong Yang, Shengli Wang, Fengqing Ren, Yuhong
- 机构：E China Univ Sci & Technol;Chinese Acad Sci
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2016
- 关键词：ginsenosides self-assembly Pichia pastoris dammarenediol-II
- Engineering Saccharomyces cerevisiae for Hyperproduction of β-Amyrin by Mitigating the Inhibition Effect of Squalene on β-Amyrin Synthase被引量：35收藏
- 作者：Du, Meng-Meng Zhu, Zhan-Tao Zhang, Ge-Ge Zhao, Yun-Qiu Gao, Bei Tao, Xin-Yi Liu, Min Ren, Yu-Hong Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2022
- 关键词：beta-amyrin squalene inhibition peroxisome compartmentalization competition pathway Saccharomyces cerevisiae
- Efficient production of (R)-(-)-mandelic acid in biphasic system by immobilized recombinant E. coli被引量：32收藏
- 作者：Ni, Kefeng Wang, Hualei Zhao, Li Zhang, Minjie Zhang, Siyuan Ren, Yuhong Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Biotechnology 2013
- 关键词：Hydrolysis - Molecular biology - Solutions - Organic acids - Cell culture - Iron oxides - Escherichia coli - Cells
- Characterization and engineering control of the effects of reactive oxygen species on the conversion of sterols to steroid synthons in Mycobacterium neoaurum被引量：29收藏
- 作者：Sun, Wan-Ju Wang, Li Liu, Hao-Hao Liu, Yong-Jun Ren, Yu-Hong Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2019
- 关键词：Sterol catabolism Reactive oxygen species Catalase Mycothiol ErgothionEIne Mycobacteria
- Assembly of cellulases with synthetic protEIn scaffolds in vitro被引量：28收藏
- 作者：Yu, Ting Gao, Xin Ren, Yuhong Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresources and Bioprocessing 2015
- 关键词：Enzymes - Hydrolysis - Scaffolds (biology)
- 脂肪酶 RWL 催化合成二氢燕麦生物碱 D被引量：27收藏
- 作者：Chen, Jinbo Zhai, Chuntao Jin, Minrong Wu, Qian Yuan, Minjia Wang, Xuedong Ren, Yuhong Wang, Hualei
- 机构： School of Biotechnology; Shanghai Zhina Biotechnology Co.; Shanghai Qiran Biotechnology Co.; Shanghai Jinjia Technology Co.
- 来源：[1] School of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China; [2] Shanghai Zhina Biotechnology Co., Ltd., Shanghai, 201400, China; [3] Shanghai Qiran Biotechnology Co., Ltd., Shanghai, 201702, China; [4] Shanghai Jinjia Technology Co., Ltd., Shanghai, 201210, China 2026
- Enhanced itaconic acid production by self-assembly of two biosynthetic enzymes in Escherichia coli被引量：27收藏
- 作者：Yang, Zhongwei Gao, Xin Xie, Hui Wang, Fengqing Ren, Yuhong Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2017
- 关键词：itaconic acid aconitase cis-aconitate decarboxylase self-assembly protEIn-peptide interactions
- A novel technique for in situ aggregation of Gluconobacter oxydans using bio-adhesive magnetic nanoparticles被引量：26收藏
- 作者：Ni, Kefeng Lu, Huimin Wang, Cunxun Black, Kvar C. L. Wei, Dongzhi Ren, Yuhong Messersmith, Phillip B.
- 机构：E China Univ Sci & Technol;Northwestern Univ;Northwestern Univ
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2012
- 关键词：cell aggregation iron oxide nanoparticles Gluconobacter oxydans polydopamine
- Design and evaluation of a phospholipase D based drug delivery strategy of novel phosphatidyl-prodrug被引量：22收藏
- 作者：Tao, Xinyi Jia, Ning Cheng, Nenghui Ren, Yuhong Cao, Xuni Liu, Min Wei, Dongzhi Wang, Feng-Qing
- 机构：East China Univ Sci & Technol
- 来源：BIOMATERIALS 2017
- 关键词：Phosphatidyl-prodrug Phospholipase D Doxorubicin Triggered release Reverse multidrug resistance
- A new fermentation strategy for S-adenosylmethionine production in recombinant Pichia pastoris被引量：19收藏
- 作者：Zhang, Jianguo Wang, Xuedong Su, Erzheng Fang, Guochen Ren, Yuhong Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Biochemical Engineering Journal 2008
- 关键词：Cytology - Yeast - Fermentation - Bottles - Cells
- Production of enantiomerically pure (S)-β-phenylalanine and (R)-β-phenylalanine by penicillin G acylase from Escherichia coli in aqueous medium被引量：17收藏
- 作者：Li, Dengchao Cheng, Shiwei Wei, Dongzhi Ren, Yuhong Zhang, Derong
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Biotechnology Letters 2007
- 关键词：Acylation - Enantioselectivity - Enzymes - Escherichia coli - Hydrolysis
- Fe3+-induced bioinspired chitosan hydrogels for the sustained and controlled release of doxorubicin被引量：15收藏
- 作者：Zhang, Jinmao Tao, Xinyi Liu, Jianwen Wei, Dongzhi Ren, Yuhong
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol
- 来源：RSC ADVANCES 2016
- 关键词：Addition reactions - Hydrogels - Targeted drug delivery - Amino acids - Controlled drug delivery - Gelation
- Engineering diverse eubacteria promoters for robust Gene expression in Streptomyces lividans被引量：14收藏
- 作者：Zhao, Ming Wang, Shui-Ling Tao, Xin-Yi Zhao, Guo-Ling Ren, Yu-Hong Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2019
- 关键词：Streptomyces Heterologous promoter 5 '-UTR RBS sigma hrdB
- Construction and characterization of the recombinant immunotoxin RTA-4D5-KDEL targeting HER2/neu-positive cancer cells and locating the endoplasmic reticulum被引量：14收藏
- 作者：Jiao, Pengyan Zhang, Jian Dong, Yuguo Wei, Dongzhi Ren, Yuhong
- 机构：East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2018
- 关键词：Recombinant immunotoxin Ricin A HER2 Localization peptide Specific targeting Cancer cell
- A novel nitrilase from Ralstonia eutropha H16 and its application to nicotinic acid production被引量：14收藏
- 作者：Fan, Haiyang Chen, Lifeng Sun, Huihui Wang, Hualei Ren, Yuhong Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Chinese Acad Fishery Sci
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2017
- 关键词：Nitrilase Ralstonia eutropha Nicotinic acid Biotransformation
- Characterization of a novel nitrilase, BGC4, from Paraburkholderia graminis showing wide-spectrum substrate specificity, a potential versatile biocatalyst for the degradation of nitriles被引量：13收藏
- 作者：Fan, Haiyang Chen, Lifeng Sun, Huihui Wang, Hualei Liu, Qinghai Ren, Yuhong Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Chinese Acad Fishery Sci Qingdao
- 来源：BIOTECHNOLOGY LETTERS 2017
- 关键词：Biodegradation Nitrilase Paraburkholderia graminis Wide-spectrum substrate specificity
- Biosynthesis of L-Erythrose by Assembly of Two Key Enzymes in Gluconobacter oxydans被引量：11收藏
- 作者：Zou, Xingxing Lin, Jinping Mao, Xinlei Zhao, Shengyun Ren, Yuhong
- 机构：East China Univ Sci & Technol;Wuyi Univ
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2017
- 关键词：L-erythrose self-assembly G. oxydans protEIn peptide interactions
- Reversible Photocontrol of Lipase Activity by Incorporating a Photoswitch into the Lid Domain被引量：8收藏
- 作者：Liu, Ying Gao, Xin Wei, Dongzhi Ren, Yuhong
- 机构：East China Univ Sci & Technol
- 来源：CHEMPHOTOCHEM 2017
- 关键词：azobenzenes enzyme activity lipase photoisomerization photoswitch
- Facile synthesis of glutathione-functionalized Fe3O4@polydopamine for separation of GST-tagged protEIn被引量：8收藏
- 作者：Ni, Kefeng Yang, Jianbing Ren, Yuhong Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：MATERIALS LETTERS 2014
- 关键词：Polydopamine Glutathione Magnetic materials Adhesion ProtEIn separation
- SAC-TRAIL, a novel anticancer fusion protEIn: expression, purification, and functional characterization被引量：8收藏
- 作者：Zhang, Jian Dong, Wanyuan Ren, Yuhong Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2022
- 关键词：SAC TRAIL Fusion protEIn Cancer therapy Linker selection
- The expression, purification, and functional evaluation of the novel tumor suppressor fusion protEIn IL-24-CN被引量：7收藏
- 作者：Zhang, Jian Zhang, Kaizheng Ren, Yuhong Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2021
- 关键词：Interleukin-24 Contortrostatin Melanoma Apoptosis Cell adhesion
