成果/Result
- Efficient accumulation of high-value bioactive substances by carbon to nitrogen ratio regulation in marine microalgae Porphyridium purpureum被引量：89收藏
- 作者：Li, Shaohua Ji, Liang Chen, Cheng Zhao, Shuxin Sun, Meng Gao, Zhengquan Wu, Haizhen Fan, Jianhua
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Shandong Univ Technol
- 来源：BIORESOURCE TECHNOLOGY 2020
- 关键词：Porphyridium purpureum C/N ratio Phycoerythrin Exopolysaccharides Fatty acids
- Advances in the production of bioactive substances from marine unicellular microalgae Porphyridium spp.被引量：78收藏
- 作者：Li, Shaohua Ji, Liang Shi, Qianwen Wu, Haizhen Fan, Jianhua
- 机构：East China Univ Sci & Technol;Hainan Univ;East China Univ Sci & Technol
- 来源：BIORESOURCE TECHNOLOGY 2019
- 关键词：Red algae Cultivation Metabolic regulation Application Bioactive compounds
- Evaluation of Microalgae as Immunostimulants and Recombinant Vaccines for Diseases Prevention and Control in Aquaculture被引量：58收藏
- 作者：Ma, Ke Bao, Qiuwen Wu, Yue Chen, Siwei Zhao, Shuxin Wu, Haizhen Fan, Jianhua
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 2020
- 关键词：aquaculture industry microalgae immunostimulant oral vaccine polymicrobial infections
- Dietary supplementation with microalgae enhances the zebrafish growth performance by modulating immune status and gut microbiota被引量：50收藏
- 作者：Ma, Ke Chen, Siwei Wu, Yue Ma, Yiting Qiao, Haochen Fan, Jianhua Wu, Haizhen
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2022
- 关键词：Microalgae Zebrafish Growth performance Immune status Gut microbiota
- Comparative transcriptome analyses of oleaginous Botryococcus braunii race A reveal significant differences in gene expression upon cobalt enrichment 06 Biological Sciences 0601 Biochemistry and Cell Biology被引量：48收藏
- 作者：Cheng, Pengfei Zhou, Chengxu Wang, Yan Xu, Zhihui Xu, Jilin Zhou, Dongqing Zhang, Yinghui Wu, Haizhen Zhang, Xuezhi Liu, Tianzhong Tang, Ming Yang, Qiyong Yan, Xiaojun Fan, Jianhua
- 机构： College of Food and Pharmaceutical Sciences; State Key Laboratory of Bioreactor Engineering; Department of Applied Biology; Institute of Hydrobiology; Key Laboratory of Biofuels
- 来源：Biotechnology for Biofuels 2018
- 关键词：Physiology - Biochemistry - Hydrocarbons - Metabolism - Photosynthesis - Fatty acids - Gene expression - Carbon - Biosynthesis - Cytology - Physiological models
- Global regulator BldA regulates morphological differentiation and lincomycin production in Streptomyces lincolnensis被引量：46收藏
- 作者：Hou, Bingbing Tao, Liyuan Zhu, Xiaoyu Wu, Wei Guo, Meijin Ye, Jiang Wu, Haizhen Zhang, Huizhan
- 机构： State Key Laboratory of Bioreactor Engineering; Department of Applied Biology
- 来源：Applied Microbiology and Biotechnology 2018
- 关键词：Encoding (symbols) - Amino acids - Bacteria - Metabolism
- Characterization of Edwardsiella tarda rpoS: Effect on serum resistance, chondroitinase activity, biofilm formation, and autoinducer synthetases expression被引量：40收藏
- 作者：Xiao, Jingfan Wang, Qiyao Liu, Qin Xu, Lili Wang, Xin Wu, Haizhen Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied Microbiology and Biotechnology 2009
- 关键词：Biofilms - Body fluids - Gene expression
- Cloning and characterization of the ddsA gene encoding decaprenyl diphosphate synthase from Rhodobacter capsulatus B10被引量：31收藏
- 作者：Liu, Xinyi Wu, Haizhen Ye, Jiang Yuan, Qinsheng Zhang, Huizhan
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Canadian Journal of Microbiology 2006
- 关键词：Amino acids - Bacteria - Cloning - Enzymes - Gene expression - Polypeptides
- Physiological and transcriptome analysis elucidates the metabolic mechanism of versatile Porphyridium purpureum under nitrogen deprivation for exopolysaccharides accumulation被引量：26收藏
- 作者：Ji, Liang Li, Shaohua Chen, Cheng Jin, Haojie Wu, Haizhen Fan, Jianhua
- 机构：East China Univ Sci & Technol;Beijing Forestry Univ;East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2021
- 关键词：Porphyridium purpureum Nitrogen deprivation Transcriptome analysis Exopolysaccharides Polyunsaturated fatty acids Phycoerythrin
- A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors被引量：26收藏
- 作者：Zhang, Huijing Wu, Haizhen Zhang, Huizhan
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Biotechnology Letters 2007
- 关键词：Biomarkers - Cells - Cloning - Genes
- Characterization of the bagremycin biosynthetic gene cluster in Streptomyces sp. Tü 4128被引量：22收藏
- 作者：Ye, Jiang Zhu, Yunxia Hou, Bingbing Wu, Haizhen Zhang, Huizhan
- 机构： Key Laboratory of Bioreactor Engineering; Department of Applied Biology
- 来源：Bioscience, Biotechnology and Biochemistry 2019
- 关键词：Antibiotics - Biochemistry - Bacteria - Genes
- Towards green biomanufacturing of high-value recombinant protEIns using promising cell factory: Chlamydomonas rEInhardtii chloroplast被引量：21收藏
- 作者：Ma, Ke Deng, Lei Wu, Haizhen Fan, Jianhua
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Shihezi Univ
- 来源：BIORESOURCES AND BIOPROCESSING 2022
- 关键词：Chlamydomonas rEInhardtii Chloroplast Synthetic biology Green factory Selectable marker ProtEIn expression
- Developmental regulator RamRsl controls both morphological development and lincomycin biosynthesis in Streptomyces lincolnensis被引量：16收藏
- 作者：Wang, Ruida Cao, Yuan Kong, Fanjing Hou, Bingbing Zhao, Jiaqi Kang, Yajing Ye, Jiang Wu, Haizhen Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：JOURNAL OF APPLIED MICROBIOLOGY 2022
- 关键词：lincomycin biosynthesis morphology RamR Streptomyces lincolnensis transcriptional regulation
- AflQ1-Q2 represses lincomycin biosynthesis via multiple cascades in Streptomyces lincolnensis被引量：15收藏
- 作者：Wang, Ruida Zhou, Tianyu Kong, Fanjing Hou, Bingbing Ye, Jiang Wu, Haizhen Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2023
- 关键词：AflQ1-Q2 Streptomyces lincolnensis Two-component system Lincomycin biosynthesis Transcriptional regulation Aspartate metabolism
- ubiCA基因的强化表达及对大肠杆菌辅酶Q生物合成的影响被引量：15收藏
- 作者：叶江 马林 吴海珍 刘欣毅 张惠展
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2006
- 关键词：大肠杆菌 ubiCA 基因克隆 强化表达 辅酶Q
- 摘要：为调查大肠杆菌高产辅酶Q(CoQ)的可能性,首先研究大肠杆菌生物合成关键基因ubiCA强化表达对大肠杆菌CoQ产量的影响。本文构建了含有ubiCA基因的5个表达质粒,将其分别转入E.coli JM 83或BL 21(DE...
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- Molecular cloning of an insertion sequence-like element from Vibrio anguillarum and its functional identification in E. coli被引量：13收藏
- 作者：Liang, Na Wu, Haizhen Ye, Jiang Xu, Peng Zhang, Yuanxing Zhang, Huizhan
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Biotechnology Letters 2007
- 关键词：Cloning - Enzymes - Escherichia coli - Gene expression
- Characterization of a TetR-type positive regulator AtrA for lincomycin production in Streptomyces lincolnensis被引量：12收藏
- 作者：Wu, Wei Kang, Yajing Hou, Bingbing Ye, Jiang Wang, Ruida Wu, Haizhen Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 2023
- 关键词：Streptomyces lincolnensis AtrA lincomycin production regulatory pathway
- A novel method to identify yak fiber in textile被引量：12收藏
- 作者：Lu, Weimin Fei, Jing Yang, Juan Tang, Minfeng Dong, Zhenglian Zhou, Zhiping Ye, Jiang Wu, Haizhen
- 机构：Shanghai Entry Exit Inspect & Quarantine Bur;E China Univ Sci & Technol
- 来源：TEXTILE RESEARCH JOURNAL 2013
- 关键词：yak fiber deoxyribonuclEIc acid TaqMan-polymerase chain reaction identify
- DeoR regulates lincomycin production in Streptomyces lincolnensis被引量：10收藏
- 作者：Zou, Jingyun Mao, Yue Hou, Bingbing Kang, Yajing Wang, Ruida Wu, Haizhen Ye, Jiang Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 2023
- 关键词：Streptomyces lincolnensis Lincomycin DeoR family Transcriptional regulation
- LcbR1, a newly identified GntR family regulator, represses lincomycin biosynthesis in Streptomyces lincolnensis被引量：10收藏
- 作者：Wang, Ruida Zhao, Jiaqi Chen, Lei Ye, Jiang Wu, Haizhen Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2023
- 关键词：Streptomyces lincolnensis LcbR1 GntR family Lincomycin biosynthesis Transcriptional regulation
- 林可霉素生物合成基因lmbU的敲除及其遗传回补被引量：9收藏
- 作者：吕彩霞 杨捷 叶江 吴海珍 张惠展
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2008
- 关键词：同源重组 敲除 遗传回补 林可霉素生物合成
- 摘要：利用DNA同源重组原理敲除S.lincolnensisNRRL 2936中lmbU基因,筛选获得该基因缺陷株S.lincolnensisJLUa2,其不具备林可霉素生物合成能力。利用构建的不同重组质粒转化缺陷株,发现含有...
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- 荚膜红细菌接合转移系统的建立及优化被引量：8收藏
- 作者：吴海珍 李莉 张惠展 刘和
- 机构：华东理工大学生物工程学院;苏州第四制药厂
- 来源：《华东理工大学学报（自然科学版）》 2000
- 关键词：接合转移 接合效率 培养 优化 荚膜红细菌
- 摘要：从培养方式及培养时间对光合细菌Ｒｈｏｄｏｂａｃｔｅｒ ｃａｐｓｕｌａｔｕｓ的ＤＮＡ 接合转移系统进行了优化，研究了该接合转移系统的部分特性，发现接合质粒的大小、受体菌细胞膜的特性对接合效率影响较明显，这为光合细菌基因工程...
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- 基因替换及多基因共表达强化大肠杆菌辅酶Q_(10)的合成能力被引量：8收藏
- 作者：傅楠 叶江 吴海珍 孔璐 张惠展
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2008
- 关键词：大肠杆菌 基因缺失 多基因共表达 辅酶Q
- 摘要：为了强化大肠杆菌合成辅酶Q10(CoQ10)的能力,对大肠杆菌进行了相关的基因操作。通过敲除大肠杆菌染色体上的聚八异戊二烯焦磷酸合成酶基因ispB,并导入来自Gluconobactersuboxydans的聚十异戊二烯焦...
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- Cloning and expression of the polyhydroxyalkanote depolymerase gene from Pseudomonas putida, and characterization of the gene product被引量：8收藏
- 作者：Jiang, Yaqin Ye, Jiang Wu, Haizhen Zhang, Huizhan
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2004
- 关键词：cloning depolymerase polyhydroxyalkanote Pseudomonas
- Cloning and identification of the Frigocyclinone biosynthetic gene cluster from Streptomyces griseus strain NTK 97被引量：7收藏
- 作者：Mo, Jian Ye, Jiang Chen, Haozhe Hou, Bingbing Wu, Haizhen Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 2019
- 关键词：Biosynthesis frigocyclinone type II polyketide synthases C-glycosyltransferase CRISPR-Cpf1
- MarR family regulator LcbR2 activates lincomycin biosynthesis in multiple ways被引量：5收藏
- 作者：Wang, Ruida Chen, Lei Zhao, Jiaqi Gao, Yu Zhou, Tianyu Ye, Jiang Wu, Haizhen Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Tarim Univ
- 来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2025
- 关键词：Streptomyces lincolnensis LcbR2 MarR family Lincomycin biosynthesis Transcriptional regulator
- 红色糖多孢菌红霉素抗性基因的克隆与鉴定被引量：3收藏
- 作者：孙丽萍 沈琼 叶江 吴海珍 张惠展
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2002
- 关键词：鸟枪法 原生质体转化 红色糖多孢菌 红霉素抗性基因 双重抗性筛选 基因克隆 基因鉴定
- 摘要：将红色糖多孢菌 ( Saccharopolyspora erythraea)的染色体 DNA用 Pst I酶切后与载体p UWL2 0 1连接 ,转化变铅青链霉菌 ( S.lividans) TK2 3的原生质体。采用双...
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- The protocatechuic acid catabolism regulator PcaV inhibits lincomycin biosynthesis EIther directly or indirectly through the control of the expression of other regulatory or metabolic genes被引量：2收藏
- 作者：Zhou, Tianyu Wang, Ruida Jiang, Wenli Zhao, Jiaqi Ye, Jiang Wu, Haizhen Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Tarim Univ;Tarim Univ
- 来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2025
- 关键词：Streptomyces lincolnensis Metabolism regulation Lincomycin biosynthesis Transcriptional regulator Protocatechuic acid catabolism
- 大肠杆菌巴豆甜菜碱还原酶基因缺失菌株的构建被引量：1收藏
- 作者：张文刚 叶江 吴海珍 张惠展
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2004
- 关键词：同源重组 巴豆甜菜碱还原酶 基因缺失 抗性基因 突变株
- 摘要：报道了体外构建caiA基因缺失的带有卡那霉素抗性基因的5.2kb线状DNA分子,以此转化大肠杆菌JM83和BL21(DE3)株,借助于体内DNA同源重组,定向敲除了大肠杆菌中的巴豆甜菜碱还原酶编码基因caiA。经遗传稳定...
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- 大肠杆菌guaC基因的克隆及高效表达被引量：0收藏
- 作者：糜蓉娟 吴海珍 叶江 张惠展 叶勤
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2003
- 关键词：guaC E.coli MC4100 鸟苷酸还原酶 基因表达
- 摘要：以 E.coli MC41 0 0染色体 DNA为模板使用 PCR扩增技术克隆了鸟苷酸还原酶基因gua C,该基因全长 1 0 44bp,编码相对分子质量为 3 7ku的蛋白质。将该基因直接克隆于 p ET-1 6b质粒...
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