成果/Result
- Immobilization of Serratia marcescens lipase onto amino-functionalized magnetic nanoparticles for repeated use in enzymatic synthesis of Diltiazem intermediate被引量：140收藏
- 作者：Hu, Bin Pan, Jiang Yu, Hui-Lei Liu, Jian-Wen Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：PROCESS BIOCHEMISTRY 2009
- 关键词：Lipase Immobilization Magnetic nanoparticles Enzymatic biocatalytic resolution Diltiazem intermediate Biphasic reaction
- Biochemical properties and potential applications of an organic solvent-tolerant lipase isolated from Serratia marcescens ECU1010被引量：84收藏
- 作者：Zhao, Li-Li Xu, Jian-He Zhao, Jian Pan, Jiang Wang, Zhi-Long
- 机构：E China Univ Sci & Technol;Shanghai Jiao Tong Univ
- 来源：PROCESS BIOCHEMISTRY 2008
- 关键词：lipase Serratia marcescens organic solvent tolerance enantiomeric resolution chiral compounds biochemical properties
- Switching Cofactor Dependence of 7β-Hydroxysteroid Dehydrogenase for Cost-Effective Production of Ursodeoxycholic Acid被引量：66收藏
- 作者：You, Zhi-Neng Chen, Qi Shi, Shou-Cheng Zheng, Ming-Min Pan, Jiang Qian, Xiao-Long Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Suzhou Bioforany EnzyTech Co Ltd
- 来源：ACS CATALYSIS 2019
- 关键词：biocatalysis cofactor dependence small-and-smart library 7 beta-hydroxysteroid dehydrogenase molecular dynamics simulation ursodeoxycholic acid
- Significant enhancement of (R)-mandelic acid production by relieving substrate inhibition of recombinant nitrilase in toluene-water biphasic system被引量：60收藏
- 作者：Zhang, Zhi-Jun Pan, Jiang Liu, Jun-Feng Xu, Jian-He He, Yu-Cai Liu, You-Yan
- 机构：E China Univ Sci & Technol;Changzhou Univ;Guangxi Univ
- 来源：JOURNAL OF BIOTECHNOLOGY 2011
- 关键词：Aqueous-organic biphasic system Recombinant nitrilase Enantioselective hydrolysis High substrate tolerance (R)-(-)-Mandelic acid Escherichia coli whole cells
- Stereoselective synthesis of L-tert-leucine by a newly cloned leucine dehydrogenase from Exiguobacterium sibiricum被引量：58收藏
- 作者：Li, Jing Pan, Jiang Zhang, Jie Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2014
- 关键词：Leucine dehydrogenase Exiguobacterium sibiricum Reductive amination Dual enzyme co-expression L-tert-Leucine
- A thermostable and organic-solvent tolerant esterase from Pseudomonas putida ECU1011: Catalytic properties and performance in kinetic resolution of α-hydroxy acids被引量：49收藏
- 作者：Ma, Bao-Di Yu, Hui-Lei Pan, Jiang Liu, Jia-Yan Ju, Xin Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：BIORESOURCE TECHNOLOGY 2013
- 关键词：Esterase Thermostability Organic-solvent tolerance alpha-Hydroxy (carboxylic) acids Deacetylation
- Rational Engineering of Formate Dehydrogenase Substrate/Cofactor Affinity for Better Performance in NADPH Regeneration被引量：46收藏
- 作者：Jiang, He-Wen Chen, Qi Pan, Jiang Zheng, Gao-Wei Xu, Jian-He
- 机构：East China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2020
- 关键词：Binding affinity Burkholderia stabilis Formate dehydrogenase NADPH regeneration Rational engineering
- An Unusual (R)-Selective Epoxide Hydrolase with High Activity for Facile Preparation of Enantiopure Glycidyl Ethers被引量：45收藏
- 作者：Zhao, Jing Chu, Yan-Yan Li, Ai-Tao Ju, Xin Kong, Xu-Dong Pan, Jiang Tang, Yun Xu, Jian-He
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol
- 来源：ADVANCED SYNTHESIS & CATALYSIS 2011
- 关键词：enantioselectivity epoxide hydrolase glycidyl ethers kinetic resolution molecular modeling
- Biocatalytic synthesis of (R)-(-)-mandelic acid from racemic mandelonitrile by a newly isolated nitrilase-producer Alcaligenes sp ECU0401被引量：44收藏
- 作者：He, Yu Cai Xu, Jian He Xu, Yi Ouyang, Li Ming Pan, Jiang
- 机构：E China Univ Sci & Technol
- 来源：CHINESE CHEMICAL LETTERS 2007
- 关键词：mandelonitrile nitrilase (R)-(-)-mandelic acid screening Alcaligenes sp ECU0401
- Bioproduction of chiral mandelate by enantioselective deacylation of α-acetoxyphenylacetic acid using whole cells of newly isolated Pseudomonas sp ECU1011被引量：38收藏
- 作者：Ju, Xin Yu, Hui-Lei Pan, Jiang Wei, Dong-Zhi Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2010
- 关键词：Microbial esterase Pseudomonas sp. Enantioselective deacylation alpha-Acetoxyphenylacetic acid Enantiopure mandelic acid
- Catalytic resolution of (RS)-HMPC acetate by immobilized cells of Acinetobacter sp CGMCC 0789 in a medium with organic cosolvent被引量：37收藏
- 作者：Chen, Y Xu, JH Pan, J Xu, Y Shi, JB
- 机构：E China Univ Chem Technol
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2004
- 关键词：Acinetobacter sp esterase enantioselectivity enzymatic resolution 4-hydroxy-3-methyl-2-(2 '-propenyl)-2-cyclopentenone immobilized cells isopropanol
- Chemoenzymatic deracemization of chiral secondary alcohols:: Process optimization for production of (R)-1-indanol and (R)-1-phenylethanol被引量：36收藏
- 作者：Ou, Ling Xu, Yi Ludwig, Daniel Pan, Jiang Xu, Jian He
- 机构：E China Univ Sci & Technol;Univ Erlangen Nurnberg
- 来源：ORGANIC PROCESS RESEARCH & DEVELOPMENT 2008
- Evolution of Glucose Dehydrogenase for Cofactor Regeneration in Bioredox Processes with Denaturing Agents被引量：35收藏
- 作者：Qian, Wen-Zhuo Ou, Ling Li, Chun-Xiu Pan, Jiang Xu, Jian-He Chen, Qi Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol
- 来源：CHEMBIOCHEM 2020
- 关键词：biocatalysis chemical stability cofactor regeneration directed evolution glucose dehydrogenase
- Crosslinking of enzyme coaggregate with polyethyleneimine: A simple and promising method for preparing stable biocatalyst of Serratia marcescens lipase被引量：35收藏
- 作者：Pan, Jiang Kong, Xu-Dong Li, Chun-Xiu Ye, Qin Xu, Jian-He Imanaka, Tadayuki
- 机构：E China Univ Sci & Technol;Ritsumeikan Univ
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2011
- 关键词：Crosslinked enzyme coaggregate Serratia marcescens lipase Polyethyleneimine Coaggregation of enzyme trans-3-(4 '-Methoxyphenyl)glycidic acid methyl ester (MPGM)
- Biocatalytic resolution of glycidyl aryl ethers by Trichosporon loubierii:: cell/substrate ratio influences the optical purity of (R)-epoxides被引量：34收藏
- 作者：Xu, Y Xu, JH Pan, J Tang, YF
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2004
- 关键词：cell/substrate ratio enantioselective hydrolysis epoxide hydrolase glycidyl aryl ether Trichosporon loubierii
- Enzymatic synthesis of high-titer nicotinamide mononucleotide with a new nicotinamide riboside kinase and an efficient ATP regeneration system被引量：34收藏
- 作者：Qian, Xiao-Long Dai, Yi-Si Li, Chun-Xiu Pan, Jiang Xu, Jian-He Mu, Bozhong
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Suzhou Bioforany EnzyTech Co Ltd
- 来源：BIORESOURCES AND BIOPROCESSING 2022
- 关键词：Enzymatic phosphorylation Nicotinamide riboside Nicotinamide riboside kinase beta-Nicotinamide mononucleotide (NMN) ATP regeneration system
- Preparation of (R)-(-)-mandelic acid and its derivatives from racemates by enantioselective degradation with a newly isolated bacterial strain Alcaligenes sp ECU0401被引量：34收藏
- 作者：He, Yu-Cai Xu, Jian-He Pan, Jiang Ouyang, Li-Ming Xu, Yi
- 机构：E China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2008
- 关键词：(R)-(-)-mandelic acid Alcaligenes sp ECU0401 (S)-(+)-mandelic acid dehydrogenase enantioselective degradation optical resolution
- An efficient bioprocess for enzymatic production of L-menthol with high ratio of substrate to catalyst using whole cells of recombinant E. coli被引量：31收藏
- 作者：Zheng, Gao-Wei Pan, Jiang Yu, Hui-Lei Ngo-Thi, Minh-Thu Li, Chun-Xiu Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2010
- 关键词：Whole-cell biocatalysis High ratio of substrate to catalyst High substrate loading Esterase L-Menthol
- Efficient synthesis of a statin precursor in high space-time yield by a new aldehyde-tolerant aldolase identified from Lactobacillus brevis被引量：30收藏
- 作者：Jiao, Xue-Cheng Pan, Jiang Xu, Guo-Chao Kong, Xu-Dong Chen, Qi Zhang, Zhi-Jun Xu, Jian-He
- 机构：E China Univ Sci & Technol; E China Univ Sci & Technol
- 来源：CATALYSIS SCIENCE & TECHNOLOGY 2015
- 关键词：Crystal structure - Stereochemistry - Acetaldehyde - Hydrogen bonds - Ketones - Stability - Substrates
- Highly Efficient Synthesis of (R)-3-Quinuclidinol in a Space-Time Yield of 916 g L-1 d-1 Using a New Bacterial Reductase ArQR被引量：30收藏
- 作者：Zhang, Wen-Xia Xu, Guo-Chao Huang, Lei Pan, Jiang Yu, Hui-Lei Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：ORGANIC LETTERS 2013
- Immobilization of glycolate oxidase from Medicago falcata on magnetic nanoparticles for application in biosynthesis of glyoxylic acid被引量：27收藏
- 作者：Zhu, Hong Pan, Jiang Hu, Bin Yu, Hui-Lei Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2009
- 关键词：Glycolate oxidase Medicago falcata Linn. Magnetic nanoparticles Immobilization Glyoxylic acid
- Remodeling the Homologous Recombination Mechanism of Yarrowia lipolytica for High-Level Biosynthesis of Squalene被引量：26收藏
- 作者：Xu, Man Yang, Nan Pan, Jiang Hua, Qiang Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2024
- 关键词：homologous recombination squalene Yarrowia lipolytica metabolic engineering acetyl coenzyme A
- Efficient Stereoselective Synthesis of Structurally Diverse γ- and δ-Lactones Using an Engineered Carbonyl Reductase被引量：26收藏
- 作者：Chen, Meng Zhang, Xiao-Yan Xing, Chen-Guang Zhang, Chao Zheng, Yu-Cong Pan, Jiang Xu, Jian-He Bai, Yun-Peng
- 机构：East China Univ Sci & Technol;Xiamen Oamic Biotech Co Ltd
- 来源：CHEMCATCHEM 2019
- 关键词：Asymmetric reduction directed evolution chiral lactones carbonyl reductase biocatalysis
- Immobilization of Bacillus subtilis esterase by simple cross-linking for enzymatic resolution of DL-menthyl acetate被引量：26收藏
- 作者：Zheng, Gao-Wei Yu, Hui-Lei Li, Chun-Xiu Pan, Jiang Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2011
- 关键词：Bacillus subtilis esterase Cross-linked enzyme aggregates Hydrolysis Immobilization L-Menthol
- Efficient production of (R)-(-)-mandelic acid using glutaraldehyde cross-linked Escherichia coli cells expressing Alcaligenes sp nitrilase被引量：24收藏
- 作者：Zhang, Zhi-Jun Pan, Jiang Li, Chun-Xiu Yu, Hui-Lei Zheng, Gao-Wei Ju, Xin Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2014
- 关键词：Immobilized cells Alcaligenes sp nitrilase Glutaraldehyde cross-linking Enantioselective hydrolysis (R)-(-)-Mandelic acid
- Optimization and Scale-up of a Bioreduction Process for Preparation of Ethyl (S)-4-Chloro-3-hydroxybutanoate被引量：23收藏
- 作者：Pan, Jiang Zheng, Gao-Wei Ye, Qin Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：ORGANIC PROCESS RESEARCH & DEVELOPMENT 2014
- 关键词：Coenzymes
- Efficient Synthesis of 12-Oxochenodeoxycholic Acid Using a 12α-Hydroxysteroid Dehydrogenase from Rhodococcus ruber被引量：23收藏
- 作者：Shi, Shou-Cheng You, Zhi-Neng Zhou, Ke Chen, Qi Pan, Jiang Qian, Xiao-Long Xu, Jian-He Li, Chun-Xiu
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Suzhou Bioforany EnzyTech Co Ltd
- 来源：ADVANCED SYNTHESIS & CATALYSIS 2019
- 关键词：biocatalysis 12 alpha-hydroxysteroid dehydrogenase NAD(+)-dependence structure-guided genome mining 12-oxochenodeoxycholic acid
- Efficient reduction of aromatic ketones with NADPH regeneration by using crude enzyme from Rhodotorula cells and mannitol as cosubstrate被引量：22收藏
- 作者：Yang, Wei Xu, Jian-He Pan, Jiang Xu, Yi Wang, Zhi-Long
- 机构：E China Univ Sci & Technol;Shanghai Jiao Tong Univ
- 来源：BIOCHEMICAL ENGINEERING JOURNAL 2008
- 关键词：biocatalysis brotransformations chromatography optimization NADPH regeneration Rhodotorula sp AS2.2241 ketoreductase
- Bioreduction of methyl o-chlorobenzoylformate at 500 g L-1 without external cofactors for efficient production of enantiopure clopidogrel intermediate被引量：22收藏
- 作者：Ni, Yan Pan, Jiang Ma, Hong-Min Li, Chun-Xiu Zhang, Jie Zheng, Gao-Wei Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：TETRAHEDRON LETTERS 2012
- 关键词：Asymmetric reduction Aldo-keto reductase Whole-cell catalyst Methyl (R)-o-chloromandelate Clopidogrel
- Significantly enhanced production of recombinant nitrilase by optimization of culture conditions and glycerol feeding被引量：21收藏
- 作者：Liu, Jun-Feng Zhang, Zhi-Jun Li, Ai-Tao Pan, Jiang Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2011
- 关键词：Nitrilase Optimization Fermentation Glycerol feeding Recombinant Escherichia coli
