成果/Result
- New opportunities for biocatalysis: driving the synthesis of chiral chemicals被引量：148收藏
- 作者：Zheng, Gao-Wei Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：CURRENT OPINION IN BIOTECHNOLOGY 2011
- 关键词：Stereochemistry - Biocatalysts - Biochemical engineering
- Recent progress on deep eutectic solvents in biocatalysis被引量：136收藏
- 作者：Xu, Pei Zheng, Gao-Wei Zong, Min-Hua Li, Ning Lou, Wen-Yong
- 机构：South China Univ Technol;South China Univ Technol;East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2017
- 关键词：Deep eutectic solvents Biocatalysis Catalysts Biodegradability Influence
- Whole-Cell Biocatalytic Processes with Ionic Liquids被引量：132收藏
- 作者：Xu, Pei Zheng, Gao-Wei Du, Peng-Xuan Zong, Min-Hua Lou, Wen-Yong
- 机构： State Key Laboratory of Pulp and Paper Engineering; State Key Laboratory of Bioreactor Engineering; Laboratory of Applied Biocatalysis
- 来源：ACS Sustainable Chemistry and Engineering 2016
- 关键词：Catalysis - Cells - Cytology - Biodegradability - Ionic liquids - Enzymes
- Asymmetric Amination of Secondary Alcohols by using a Redox-Neutral Two-Enzyme Cascade被引量：112收藏
- 作者：Chen, Fei-Fei Liu, You-Yan Zheng, Gao-Wei Xu, Jian-He
- 机构：E China Univ Sci & Technol;Guangxi Univ;Guangxi Acad Sci
- 来源：CHEMCATCHEM 2015
- 关键词：amine dehydrogenase asymmetric catalysis biotransformations cascade reaction chiral amines
- Reshaping the Active Pocket of Amine Dehydrogenases for Asymmetric Synthesis of Bulky Aliphatic Amines被引量：102收藏
- 作者：Chen, Fei-Fei Zheng, Gao-Wei Liu, Lei Li, Hao Chen, Qi Li, Fu-Long Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol
- 来源：ACS CATALYSIS 2018
- 关键词：amine dehydrogenase reductive amination chiral amine protEIn engineering biocatalysis substrate scope
- Development of an Engineered Ketoreductase with Simultaneously Improved Thermostability and Activity for Making a Bulky Atorvastatin Precursor被引量：101收藏
- 作者：Gong, Xu-Min Qin, Zhen Li, Fu-Long Zeng, Bu-Bing Zheng, Gao-Wei Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：ACS CATALYSIS 2019
- 关键词：biocatalysis asymmetric reduction ketoreductase protEIn engineering thermostabilization atorvastatin precursor
- Preparation of Structurally Diverse Chiral Alcohols by Engineering Ketoreductase CgKR1被引量：82收藏
- 作者：Zheng, Gao-Wei Liu, Yuan-Yang Chen, Qi Huang, Lei Yu, Hui-Lei Lou, Wen-Yong Li, Chun-Xiu Bai, Yun-Peng Li, Ai-Tao Xu, Jian-He
- 机构：East China Univ Sci & Technol;South China Univ Technol;Max Planck Inst Kohlenforsch
- 来源：ACS CATALYSIS 2017
- 关键词：asymmetric reduction ketoreductase chiral alcohol protEIn engineering substrate specificity biocatalysis
- A Motif-Based Deep Learning Tool for the Identification of Unusual NADH-Dependent Imine Reductases被引量：73收藏
- 作者：Shen, Xin-Yuan Wu, Yu-Xuan Ma, Zhi-Feng Yi, Xiao Shi, Min Zhu, Zhen-Yu Jin, Tian Hu, Xiao-Yu Huang, Zi-Yi Gao, Yun-Fei Chen, Qi Yu, Hui-Qun Xu, Jian-He Fan, Gui-Sheng Zheng, Gao-Wei
- 机构： State Key Laboratory of Bioreactor Engineering; School of Information Science and Engineering
- 来源：SSRN 2025
- 关键词：Amination - Enzyme immobilization - Enzyme inhibition - Enzyme kinetics - Hydrolases - Lipases
- Enantioselective Synthesis of Chiral Vicinal Amino Alcohols Using Amine Dehydrogenases被引量：67收藏
- 作者：Chen, Fei-Fei Cosgrove, Sebastian C. Birmingham, William R. Mangas-Sanchez, Juan Citoler, Joan Thompson, Matthew P. Zheng, Gao-Wei Xu, Jian-He Turner, Nicholas J.
- 机构：East China Univ Sci & Technol;Univ Manchester;EnginZyme AB
- 来源：ACS CATALYSIS 2019
- 关键词：amine dehydrogenase biocatalysis chiral amino alcohols directed evolution reductive amination
- Chemical modulators for enzymatic reactions被引量：66收藏
- 作者：Xu, Jian-He Zheng, Gao-Wei Luo, Xiao-Jing
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology
- 来源：RSC Green Chemistry 2015
- One-Pot Enzyme Cascade for Controlled Synthesis of Furancarboxylic Acids from 5-Hydroxymethylfurfural by H2O2 Internal Recycling被引量：58收藏
- 作者：Jia, Hao-Yu Zong, Min-Hua Zheng, Gao-Wei Li, Ning
- 机构：South China Univ Technol;East China Univ Sci & Technol
- 来源：CHEMSUSCHEM 2019
- 关键词：biocatalysis biomass dehydrogenases heterocycles oxidases
- Discovery of an Imine Reductase for Reductive Amination of Carbonyl Compounds with Sterically Challenging Amines被引量：54收藏
- 作者：Chen, Fei-Fei He, Xue-Feng Zhu, Xin-Xin Zhang, Zhi Shen, Xin-Yuan Chen, Qi Xu, Jian-He Turner, Nicholas J. Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol;Univ Manchester
- 来源：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2023
- 关键词：Carbonyl compounds - Nucleophiles - Substrates - Synthesis (chemical)
- Rational Engineering of Formate Dehydrogenase Substrate/Cofactor Affinity for Better Performance in NADPH Regeneration被引量：46收藏
- 作者：Jiang, He-Wen Chen, Qi Pan, Jiang Zheng, Gao-Wei Xu, Jian-He
- 机构：East China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2020
- 关键词：Binding affinity Burkholderia stabilis Formate dehydrogenase NADPH regeneration Rational engineering
- Development of an engineered thermostable amine dehydrogenase for the synthesis of structurally diverse chiral amines被引量：44收藏
- 作者：Liu, Lei Wang, Dong-Hao Chen, Fei-Fei Zhang, Zhi-Jun Chen, Qi Xu, Jian-He Wang, Zhi-Long Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol;Shanghai Jiao Tong Univ
- 来源：CATALYSIS SCIENCE & TECHNOLOGY 2020
- 关键词：Ammonia - Amino acids - Amines
- Asymmetric Reductive Amination of Structurally Diverse Ketones with Ammonia Using a Spectrum-Extended Amine Dehydrogenase被引量：41收藏
- 作者：Wang, Dong-Hao Chen, Qi Yin, Sai-Nan Ding, Xu-Wei Zheng, Yu-Cong Zhang, Zhi Zhang, Yu-Hui Chen, Fei-Fei Xu, Jian-He Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：ACS CATALYSIS 2021
- 关键词：< em > reductive amination < em > < em > amine dehydrogenase < em > < em > protEIn engineering < em > < em > substrate scope < em > < em > chiral amine < em >
- Efficient production of succinic acid in engineered Escherichia coli strains controlled by anaerobically-induced nirB promoter using sweet potato waste hydrolysate被引量：36收藏
- 作者：Huang, Meihua Cheng, Jie Chen, Peng Zheng, Gaowei Wang, Dan Hu, Yuanliang
- 机构：Chongqing Univ;East China Univ Sci & Technol;Hubei Normal Univ
- 来源：JOURNAL OF ENVIRONMENTAL MANAGEMENT 2019
- 关键词：Anaerobically-induced nirB promoter Sweet potato waste Succinic acid Engineered Escherichia coil
- Efficient production of (R)-o-chloromandelic acid by deracemization of o-chloromandelonitrile with a new nitrilase mined from Labrenzia aggregata被引量：32收藏
- 作者：Zhang, Chen-Sheng Zhang, Zhi-Jun Li, Chun-Xiu Yu, Hui-Lei Zheng, Gao-Wei Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology
- 来源：Applied Microbiology and Biotechnology 2012
- 关键词：Data mining - Hydrolysis - Lanthanum compounds - Enantioselectivity
- Reductive Amination of Biobased Levulinic Acid to Unnatural Chiral γ-Amino Acid Using an Engineered Amine Dehydrogenase被引量：32收藏
- 作者：Cai, Rui-Feng Liu, Lei Chen, Fei-Fei Li, Aitao Xu, Jian-He Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol;Hubei Univ;East China Univ Sci & Technol
- 来源：ACS SUSTAINABLE CHEMISTRY & ENGINEERING 2020
- 关键词：biomass reductive amination amine dehydrogenase directed evolution unnatural gamma-amino acid (S)-4-aminopentanoic acid
- Highly efficient bioreduction of 2-hydroxyacetophenone to (S)- and (R)-1-phenyl-1,2-ethanediol by two substrate tolerance carbonyl reductases with cofactor regeneration被引量：32收藏
- 作者：Cui, Zhi-Mei Zhang, Jian-Dong Fan, Xiao-Jun Zheng, Gao-Wei Chang, Hong-Hong Wei, Wen-Long
- 机构：Taiyuan Univ Technol;East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2017
- 关键词：Highly enantioselective Carbonyl reductase 2-Hydroxyacetophenone Chiral 1-phenyl-1,2-ethanediol Cofactor regeneration
- Efficient production of l-menthol in a two-phase system with SDS using an immobilized bacillus subtilis esterase被引量：32收藏
- 作者：Pan, Jiang Dang, Ngoc-Duy Zheng, Gao-Wei Cheng, Bo Ye, Qin Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology; Sunflower Pharmaceutical Group Company Limited
- 来源：Bioresources and Bioprocessing 2014
- 关键词：Cloning - Crosslinking - Enantioselectivity - Enzymatic hydrolysis - Enzyme activity - Enzyme inhibition - Esters - Sulfur compounds
- De Novo Multienzyme Synthetic Pathways for Lactic Acid Production被引量：31收藏
- 作者：Ding, Xu-Wei Rong, Jing Pan, Ze-Peng Zhu, Xin-Xin Zhu, Zhen-Yu Chen, Qi Zhang, Zhi-Jun Xu, Jian-He Li, Chun-Xiu Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol
- 来源：ACS CATALYSIS 2024
- 关键词：biocatalysis C1 compound lacticacid multienzyme cascade protEIn engineering
- Immobilization of Bacillus subtilis esterase by simple cross-linking for enzymatic resolution of dl-menthyl acetate被引量：31收藏
- 作者：Zheng, Gao-Wei Yu, Hui-Lei Li, Chun-Xiu Pan, Jiang Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Bioprocessing
- 来源：Journal of Molecular Catalysis B: Enzymatic 2011
- 关键词：Bacteriology - Monoterpenes - Nitrogen compounds - Cloning - Esters - Enzyme immobilization
- Engineering of a novel carbonyl reductase with coenzyme regeneration in E. coli for efficient biosynthesis of enantiopure chiral alcohols被引量：29收藏
- 作者：Wei, Ping Gao, Jia-Xin Zheng, Gao-Wei Wu, Hong Zong, Min-Hua Lou, Wen-Yong
- 机构：S China Univ Technol;E China Univ Sci & Technol;S China Univ Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2016
- 关键词：Asymmetric reduction Whole-cell biocatalysis Acetobacter sp GAcCR (S)-CHBE Chiral alcohols
- A Novel (R)-Imine Reductase from Paenibacillus lactis for Asymmetric Reduction of 3H-Indoles被引量：29收藏
- 作者：Li, Hao Zhang, Guang-Xiang Li, Liu-Mei Ou, Yu-Shi Wang, Ming-Yang Li, Chun-Xiu Zheng, Gao-Wei Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：CHEMCATCHEM 2016
- 关键词：asymmetric reduction biocatalysis imine reductase indoles indolines
- Bioamination of alkane with ammonium by an artificially designed multienzyme cascade被引量：28收藏
- 作者：Yu, Hui-Lei Li, Tuo Chen, Fei-Fei Luo, Xiao-Jing Li, Aitao Yang, Chao Zheng, Gao-Wei Xu, Jian-He
- 机构：East China Univ Sci & Technol;Hubei Univ;Nankai Univ;Nankai Univ
- 来源：METABOLIC ENGINEERING 2018
- 关键词：Systems biocatalysis Cascade reaction C-H amination Cyclohexylamine
- Engineered Imine Reductase for Larotrectinib Intermediate Manufacture被引量：28收藏
- 作者：Chen, Qi Li, Bo-Bo Zhang, Lilan Chen, Xin-Ru Zhu, Xin-Xin Chen, Fei-Fei Shi, Min Chen, Chun-Chi Yang, Yu Guo, Rey-Ting Liu, Weidong Xu, Jian-He Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol;Hubei Univ;Chinese Acad Sci
- 来源：ACS CATALYSIS 2022
- 关键词：asymmetric reduction biocatalysis directed evolution imine reductase larotrectinib
- Enantioselective production of (S)-1-phenyl-1,2-ethanediol from dicarboxyesters by recombinant Bacillus subtilis esterase被引量：27收藏
- 作者：Tian, Xin Zheng, Gao-Wei Li, Chun-Xiu Wang, Zhi-Long Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology; School of Pharmacy
- 来源：Journal of Molecular Catalysis B: Enzymatic 2011
- 关键词：Chlorine compounds - Enantioselectivity - Liquid crystal displays - Enzymes - Esters - Bacteriology - Hydrolysis
- An efficient bioprocess for enzymatic production of l-menthol with high ratio of substrate to catalyst using whole cells of recombinant E. coli被引量：26收藏
- 作者：Zheng, Gao-Wei Pan, Jiang Yu, Hui-Lei Ngo-Thi, Minh-Thu Li, Chun-Xiu Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Bioprocessing
- 来源：Journal of Biotechnology 2010
- 关键词：Bacteriology - Cloning - Escherichia coli - Monoterpenes - Esters - Cytology - Gene encoding - Catalysis - Enzymes - Loading
- Myoglobin-Catalyzed Efficient In Situ Regeneration of NAD(P)+ and ThEIr Synthetic Biomimetic for Dehydrogenase-Mediated Oxidations被引量：26收藏
- 作者：Jia, Hao-Yu Zong, Min-Hua Zheng, Gao-Wei Li, Ning
- 机构：South China Univ Technol;East China Univ Sci & Technol
- 来源：ACS CATALYSIS 2019
- 关键词：cofactor regeneration dehydrogenases enzyme catalysis enzyme catalytic promiscuity oxidation
- An Ammonium-Formate-Driven Trienzymatic Cascade for ω-Transaminase-Catalyzed (R)-Selective Amination被引量：25收藏
- 作者：Chen, Fei-Fei Zhang, Yu-Hui Zhang, Zhi-Jun Liu, Lei Wu, Jian-Ping Xu, Jian-He Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol;Zhejiang Univ
- 来源：JOURNAL OF ORGANIC CHEMISTRY 2019
- 关键词：Amino acids - Amines - Amination
