成果/Result
- Inhibitor degradation and lipid accumulation potentials of oleaginous yeast Trichosporon cutaneum using lignocellulose feedstock被引量：80收藏
- 作者：Wang, Juan Gao, Qiuqiang Zhang, Huizhan Bao, Jie
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCE TECHNOLOGY 2016
- 关键词：Trichosporon cutaneum ACCC 20271 Lipid Biodiesel Lignocellulose Inhibitor degradation
- Global regulator BldA regulates morphological differentiation and lincomycin production in Streptomyces lincolnensis被引量：46收藏
- 作者：Hou, Bingbing Tao, Liyuan Zhu, Xiaoyu Wu, Wei Guo, Meijin Ye, Jiang Wu, Haizhen Zhang, Huizhan
- 机构： State Key Laboratory of Bioreactor Engineering; Department of Applied Biology
- 来源：Applied Microbiology and Biotechnology 2018
- 关键词：Encoding (symbols) - Amino acids - Bacteria - Metabolism
- Cloning and characterization of the ddsA gene encoding decaprenyl diphosphate synthase from Rhodobacter capsulatus B10被引量：31收藏
- 作者：Liu, Xinyi Wu, Haizhen Ye, Jiang Yuan, Qinsheng Zhang, Huizhan
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Canadian Journal of Microbiology 2006
- 关键词：Amino acids - Bacteria - Cloning - Enzymes - Gene expression - Polypeptides
- Identification of BagI as a positive transcriptional regulator of bagremycin biosynthesis in engineered Streptomyces sp. Tü 4128被引量：30收藏
- 作者：Liu, Feng Xu, Dakui Zhang, Yuchen Zhu, Yunxia Ye, Jiang Zhang, Huizhan
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Microbiological Research 2015
- 关键词：Bacteria - Metabolism - Polymerase chain reaction - Fungi - Biochemistry
- A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors被引量：26收藏
- 作者：Zhang, Huijing Wu, Haizhen Zhang, Huizhan
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Biotechnology Letters 2007
- 关键词：Biomarkers - Cells - Cloning - Genes
- Genetic engineering of the branched-chain fatty acid biosynthesis pathway to enhance surfactin production from Bacillus subtilis被引量：22收藏
- 作者：Jing, Ya-Fei Wei, Hao-Xun Liu, Fang-Fang Liu, Yi-Fan Zhou, Lei Liu, Jin-Feng Yang, Shi-Zhong Zhang, Hui-Zhan Mu, Bo-Zhong
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Minist Educ;Shanghai Collaborat Innovat Ctr Biomfg Technol
- 来源：BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 2023
- 关键词：Bacillus subtilis fatty acid gene expression metabolic engineering precursor biosynthesis surfactin
- Characterization of the bagremycin biosynthetic gene cluster in Streptomyces sp. Tü 4128被引量：22收藏
- 作者：Ye, Jiang Zhu, Yunxia Hou, Bingbing Wu, Haizhen Zhang, Huizhan
- 机构： Key Laboratory of Bioreactor Engineering; Department of Applied Biology
- 来源：Bioscience, Biotechnology and Biochemistry 2019
- 关键词：Antibiotics - Biochemistry - Bacteria - Genes
- Cloning and characterization of a novel tyrosine ammonia lyase-encoding gene involved in bagremycins biosynthesis in Streptomyces sp.被引量：19收藏
- 作者：Zhu, Yunxia Liao, Siyi Ye, Jiang Zhang, Huizhan
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2012
- 关键词：Aromatic amino acid ammonia lyase Bagremycins trans-coumaric acid Streptomyces Tyrosine ammonia lyase
- Discovery of 16-Demethylrifamycins by Removing the Predominant Polyketide Biosynthesis Pathway in Micromonospora sp. Strain TP-A0468被引量：17收藏
- 作者：Zhou, Qiang Luo, Guang-Cai Zhang, Huizhan Tang, Gang-Li
- 机构：East China Univ Sci & Technol;Chinese Acad Sci
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2019
- 关键词：ansamycins cross talk genome mining metabolic flux redirection rifamycin
- Developmental regulator RamRsl controls both morphological development and lincomycin biosynthesis in Streptomyces lincolnensis被引量：16收藏
- 作者：Wang, Ruida Cao, Yuan Kong, Fanjing Hou, Bingbing Zhao, Jiaqi Kang, Yajing Ye, Jiang Wu, Haizhen Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：JOURNAL OF APPLIED MICROBIOLOGY 2022
- 关键词：lincomycin biosynthesis morphology RamR Streptomyces lincolnensis transcriptional regulation
- AflQ1-Q2 represses lincomycin biosynthesis via multiple cascades in Streptomyces lincolnensis被引量：15收藏
- 作者：Wang, Ruida Zhou, Tianyu Kong, Fanjing Hou, Bingbing Ye, Jiang Wu, Haizhen Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2023
- 关键词：AflQ1-Q2 Streptomyces lincolnensis Two-component system Lincomycin biosynthesis Transcriptional regulation Aspartate metabolism
- ubiCA基因的强化表达及对大肠杆菌辅酶Q生物合成的影响被引量：15收藏
- 作者：叶江 马林 吴海珍 刘欣毅 张惠展
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2006
- 关键词：大肠杆菌 ubiCA 基因克隆 强化表达 辅酶Q
- 摘要：为调查大肠杆菌高产辅酶Q(CoQ)的可能性,首先研究大肠杆菌生物合成关键基因ubiCA强化表达对大肠杆菌CoQ产量的影响。本文构建了含有ubiCA基因的5个表达质粒,将其分别转入E.coli JM 83或BL 21(DE...
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- Converting Chemical Oxygen Demand (COD) of Cellulosic Ethanol Fermentation Wastewater into Microbial Lipid by Oleaginous Yeast Trichosporon cutaneum被引量：14收藏
- 作者：Wang, Juan Hu, Mingshan Zhang, Huizhan Bao, Jie
- 机构：East China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2017
- 关键词：Trichosporon cutaneum Cellulosic ethanol fermentation wastewater Chemical oxygen demand (COD) Microbial lipid
- Molecular cloning of an insertion sequence-like element from Vibrio anguillarum and its functional identification in E. coli被引量：13收藏
- 作者：Liang, Na Wu, Haizhen Ye, Jiang Xu, Peng Zhang, Yuanxing Zhang, Huizhan
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Biotechnology Letters 2007
- 关键词：Cloning - Enzymes - Escherichia coli - Gene expression
- Characterization of a TetR-type positive regulator AtrA for lincomycin production in Streptomyces lincolnensis被引量：12收藏
- 作者：Wu, Wei Kang, Yajing Hou, Bingbing Ye, Jiang Wang, Ruida Wu, Haizhen Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 2023
- 关键词：Streptomyces lincolnensis AtrA lincomycin production regulatory pathway
- LcbR1, a newly identified GntR family regulator, represses lincomycin biosynthesis in Streptomyces lincolnensis被引量：10收藏
- 作者：Wang, Ruida Zhao, Jiaqi Chen, Lei Ye, Jiang Wu, Haizhen Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2023
- 关键词：Streptomyces lincolnensis LcbR1 GntR family Lincomycin biosynthesis Transcriptional regulation
- DeoR regulates lincomycin production in Streptomyces lincolnensis被引量：10收藏
- 作者：Zou, Jingyun Mao, Yue Hou, Bingbing Kang, Yajing Wang, Ruida Wu, Haizhen Ye, Jiang Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 2023
- 关键词：Streptomyces lincolnensis Lincomycin DeoR family Transcriptional regulation
- 林可霉素生物合成基因lmbU的敲除及其遗传回补被引量：9收藏
- 作者：吕彩霞 杨捷 叶江 吴海珍 张惠展
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2008
- 关键词：同源重组 敲除 遗传回补 林可霉素生物合成
- 摘要：利用DNA同源重组原理敲除S.lincolnensisNRRL 2936中lmbU基因,筛选获得该基因缺陷株S.lincolnensisJLUa2,其不具备林可霉素生物合成能力。利用构建的不同重组质粒转化缺陷株,发现含有...
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- Characterization of Inulin Hydrolyzing Enzyme(s) in Oleaginous Yeast Trichosporon cutaneum in Consolidated Bioprocessing of Microbial Lipid Fermentation被引量：8收藏
- 作者：Wang, Juan Zhang, Huizhan Bao, Jie
- 机构：E China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2015
- 关键词：Inulin hydrolyzing enzyme Trichosporon cutaneum Dry cell wEIght Lipid production Consolidated bioprocessing
- 荚膜红细菌接合转移系统的建立及优化被引量：8收藏
- 作者：吴海珍 李莉 张惠展 刘和
- 机构：华东理工大学生物工程学院;苏州第四制药厂
- 来源：《华东理工大学学报（自然科学版）》 2000
- 关键词：接合转移 接合效率 培养 优化 荚膜红细菌
- 摘要：从培养方式及培养时间对光合细菌Ｒｈｏｄｏｂａｃｔｅｒ ｃａｐｓｕｌａｔｕｓ的ＤＮＡ 接合转移系统进行了优化，研究了该接合转移系统的部分特性，发现接合质粒的大小、受体菌细胞膜的特性对接合效率影响较明显，这为光合细菌基因工程...
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- 基因替换及多基因共表达强化大肠杆菌辅酶Q_(10)的合成能力被引量：8收藏
- 作者：傅楠 叶江 吴海珍 孔璐 张惠展
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2008
- 关键词：大肠杆菌 基因缺失 多基因共表达 辅酶Q
- 摘要：为了强化大肠杆菌合成辅酶Q10(CoQ10)的能力,对大肠杆菌进行了相关的基因操作。通过敲除大肠杆菌染色体上的聚八异戊二烯焦磷酸合成酶基因ispB,并导入来自Gluconobactersuboxydans的聚十异戊二烯焦...
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- Cloning and expression of the polyhydroxyalkanote depolymerase gene from Pseudomonas putida, and characterization of the gene product被引量：8收藏
- 作者：Jiang, Yaqin Ye, Jiang Wu, Haizhen Zhang, Huizhan
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2004
- 关键词：cloning depolymerase polyhydroxyalkanote Pseudomonas
- Cloning and identification of the Frigocyclinone biosynthetic gene cluster from Streptomyces griseus strain NTK 97被引量：7收藏
- 作者：Mo, Jian Ye, Jiang Chen, Haozhe Hou, Bingbing Wu, Haizhen Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 2019
- 关键词：Biosynthesis frigocyclinone type II polyketide synthases C-glycosyltransferase CRISPR-Cpf1
- MarR family regulator LcbR2 activates lincomycin biosynthesis in multiple ways被引量：5收藏
- 作者：Wang, Ruida Chen, Lei Zhao, Jiaqi Gao, Yu Zhou, Tianyu Ye, Jiang Wu, Haizhen Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Tarim Univ
- 来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2025
- 关键词：Streptomyces lincolnensis LcbR2 MarR family Lincomycin biosynthesis Transcriptional regulator
- 红色糖多孢菌红霉素抗性基因的克隆与鉴定被引量：3收藏
- 作者：孙丽萍 沈琼 叶江 吴海珍 张惠展
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2002
- 关键词：鸟枪法 原生质体转化 红色糖多孢菌 红霉素抗性基因 双重抗性筛选 基因克隆 基因鉴定
- 摘要：将红色糖多孢菌 ( Saccharopolyspora erythraea)的染色体 DNA用 Pst I酶切后与载体p UWL2 0 1连接 ,转化变铅青链霉菌 ( S.lividans) TK2 3的原生质体。采用双...
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- The protocatechuic acid catabolism regulator PcaV inhibits lincomycin biosynthesis EIther directly or indirectly through the control of the expression of other regulatory or metabolic genes被引量：2收藏
- 作者：Zhou, Tianyu Wang, Ruida Jiang, Wenli Zhao, Jiaqi Ye, Jiang Wu, Haizhen Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Tarim Univ;Tarim Univ
- 来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2025
- 关键词：Streptomyces lincolnensis Metabolism regulation Lincomycin biosynthesis Transcriptional regulator Protocatechuic acid catabolism
- 大肠杆菌巴豆甜菜碱还原酶基因缺失菌株的构建被引量：1收藏
- 作者：张文刚 叶江 吴海珍 张惠展
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2004
- 关键词：同源重组 巴豆甜菜碱还原酶 基因缺失 抗性基因 突变株
- 摘要：报道了体外构建caiA基因缺失的带有卡那霉素抗性基因的5.2kb线状DNA分子,以此转化大肠杆菌JM83和BL21(DE3)株,借助于体内DNA同源重组,定向敲除了大肠杆菌中的巴豆甜菜碱还原酶编码基因caiA。经遗传稳定...
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- 造血干细胞(CD34^+)表达谱数据分析被引量：1收藏
- 作者：陆炯 韩泽广 张惠展
- 机构：华东理工大学生物反应器工程国家重点实验室;国家人类基因组南方研究中心
- 来源：《华东理工大学学报（自然科学版）》 2005
- 关键词：造血干细胞 CD34^+ 生物信息学 转录组 EST SAGE
- 摘要：造血干细胞分化的潜能及自我更新的分子机制是造血干细胞研究中最重要的领域。通过对来自造血干细胞(CD 34+)的EST(Expressed Sequence T ags)和SAGE(Serial A nalysis of ...
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- Revealing and manipulating the LmbU-mediated connection between lincomycin production and the ectABCD gene cluster in S. lincolnensis被引量：0收藏
- 作者：Mao, Yue Ye, Jiang Wang, Ruida Wu, Haizhen Zhang, Huizhan
- 机构：East China Univ Sci & Technol;Tarim Univ
- 来源：PROCESS BIOCHEMISTRY 2026
- 关键词：Streptomyces lincolnensis Lincomycin LmbU Ectoine/hydroxyectoine Osmotic regulation
- 大肠杆菌guaC基因的克隆及高效表达被引量：0收藏
- 作者：糜蓉娟 吴海珍 叶江 张惠展 叶勤
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2003
- 关键词：guaC E.coli MC4100 鸟苷酸还原酶 基因表达
- 摘要：以 E.coli MC41 0 0染色体 DNA为模板使用 PCR扩增技术克隆了鸟苷酸还原酶基因gua C,该基因全长 1 0 44bp,编码相对分子质量为 3 7ku的蛋白质。将该基因直接克隆于 p ET-1 6b质粒...
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