成果/Result
- New opportunities for biocatalysis: driving the synthesis of chiral chemicals被引量：148收藏
- 作者：Zheng, Gao-Wei Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：CURRENT OPINION IN BIOTECHNOLOGY 2011
- 关键词：Stereochemistry - Biocatalysts - Biochemical engineering
- Asymmetric Amination of Secondary Alcohols by using a Redox-Neutral Two-Enzyme Cascade被引量：112收藏
- 作者：Chen, Fei-Fei Liu, You-Yan Zheng, Gao-Wei Xu, Jian-He
- 机构：E China Univ Sci & Technol;Guangxi Univ;Guangxi Acad Sci
- 来源：CHEMCATCHEM 2015
- 关键词：amine dehydrogenase asymmetric catalysis biotransformations cascade reaction chiral amines
- Reshaping the Active Pocket of Amine Dehydrogenases for Asymmetric Synthesis of Bulky Aliphatic Amines被引量：102收藏
- 作者：Chen, Fei-Fei Zheng, Gao-Wei Liu, Lei Li, Hao Chen, Qi Li, Fu-Long Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol
- 来源：ACS CATALYSIS 2018
- 关键词：amine dehydrogenase reductive amination chiral amine protein engineering biocatalysis substrate scope
- Development of an Engineered Ketoreductase with Simultaneously Improved Thermostability and Activity for Making a Bulky Atorvastatin Precursor被引量：101收藏
- 作者：Gong, Xu-Min Qin, Zhen Li, Fu-Long Zeng, Bu-Bing Zheng, Gao-Wei Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：ACS CATALYSIS 2019
- 关键词：biocatalysis asymmetric reduction ketoreductase protein engineering thermostabilization atorvastatin precursor
- Preparation of Structurally Diverse Chiral Alcohols by Engineering Ketoreductase CgKR1被引量：82收藏
- 作者：Zheng, Gao-Wei Liu, Yuan-Yang Chen, Qi Huang, Lei Yu, Hui-Lei Lou, Wen-Yong Li, Chun-Xiu Bai, Yun-Peng Li, Ai-Tao Xu, Jian-He
- 机构：East China Univ Sci & Technol;South China Univ Technol;Max Planck Inst Kohlenforsch
- 来源：ACS CATALYSIS 2017
- 关键词：asymmetric reduction ketoreductase chiral alcohol protein engineering substrate specificity biocatalysis
- One-pot biocatalytic route from cycloalkanes to α,ω-dicarboxylic acids by designed Escherichia coli consortia被引量：68收藏
- 作者：Wang, Fei Zhao, Jing Li, Qian Yang, Jun Li, Renjie Min, Jian Yu, Xiaojuan Zheng, Gao-Wei Yu, Hui-Lei Zhai, Chao Acevedo-Rocha, Carlos G. Ma, Lixin Li, Aitao
- 机构：Hubei Univ;East China Univ Sci & Technol;East China Univ Sci & Technol;Biosyntia ApS
- 来源：NATURE COMMUNICATIONS 2020
- Enantioselective Synthesis of Chiral Vicinal Amino Alcohols Using Amine Dehydrogenases被引量：67收藏
- 作者：Chen, Fei-Fei Cosgrove, Sebastian C. Birmingham, William R. Mangas-Sanchez, Juan Citoler, Joan Thompson, Matthew P. Zheng, Gao-Wei Xu, Jian-He Turner, Nicholas J.
- 机构：East China Univ Sci & Technol;Univ Manchester;EnginZyme AB
- 来源：ACS CATALYSIS 2019
- 关键词：amine dehydrogenase biocatalysis chiral amino alcohols directed evolution reductive amination
- Efficient Synthesis of Chiral Indolines using an Imine Reductase from Paenibacillus lactis被引量：66收藏
- 作者：Li, Hao Luan, Zheng-Jiao Zheng, Gao-Wei Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：ADVANCED SYNTHESIS & CATALYSIS 2015
- 关键词：asymmetric hydrogenation biocatalysis chiral indolines imine reductase 3H-indoles
- Whole-Cell Biocatalytic Processes with Ionic Liquids被引量：61收藏
- 作者：Xu, Pei Zheng, Gao-Wei Du, Peng-Xuan Zong, Min-Hua Lou, Wen-Yong
- 机构：S China Univ Technol;E China Univ Sci & Technol;S China Univ Technol
- 来源：ACS SUSTAINABLE CHEMISTRY & ENGINEERING 2016
- 关键词：Whole-cell Ionic liquid Biocatalysis Biocompatibility Biodegradability
- Efficient Synthesis of a Chiral Precursor for Angiotensin-Converting Enzyme (ACE) Inhibitors in High Space-Time Yield by a New Reductase without External Cofactors被引量：61收藏
- 作者：Shen, Nai-Dong Ni, Yan Ma, Hong-Min Wang, Li-Juan Li, Chun-Xiu Zheng, Gao-Wei Zhang, Jie Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：ORGANIC LETTERS 2012
- One-Pot Enzyme Cascade for Controlled Synthesis of Furancarboxylic Acids from 5-Hydroxymethylfurfural by H2O2 Internal Recycling被引量：58收藏
- 作者：Jia, Hao-Yu Zong, Min-Hua Zheng, Gao-Wei Li, Ning
- 机构：South China Univ Technol;East China Univ Sci & Technol
- 来源：CHEMSUSCHEM 2019
- 关键词：biocatalysis biomass dehydrogenases heterocycles oxidases
- Stereospecific Reduction of Methyl o-Chlorobenzoylformate at 300 g?L-1 without Additional Cofactor using a Carbonyl Reductase Mined from Candida glabrata被引量：57收藏
- 作者：Ma, Hongmin Yang, Linlin Ni, Yan Zhang, Jie Li, Chun-Xiu Zheng, Gao-Wei Yang, Huaiyu Xu, Jian-He
- 机构：E China Univ Sci & Technol;Chinese Acad Sci
- 来源：ADVANCED SYNTHESIS & CATALYSIS 2012
- 关键词：bioinformatics biotransformations cofactors oxidoreductases
- Identification of an Imine Reductase for Asymmetric Reduction of Bulky Dihydroisoquinolines被引量：56收藏
- 作者：Li, Hao Tian, Ping Xu, Jian-He Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol;Chinese Acad Sci
- 来源：ORGANIC LETTERS 2017
- Discovery of an Imine Reductase for Reductive Amination of Carbonyl Compounds with Sterically Challenging Amines被引量：54收藏
- 作者：Chen, Fei-Fei He, Xue-Feng Zhu, Xin-Xin Zhang, Zhi Shen, Xin-Yuan Chen, Qi Xu, Jian-He Turner, Nicholas J. Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol;Univ Manchester
- 来源：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2023
- 关键词：Carbonyl compounds - Nucleophiles - Substrates - Synthesis (chemical)
- Efficient production of (R)-o-chloromandelic acid by deracemization of o-chloromandelonitrile with a new nitrilase mined from Labrenzia aggregata被引量：53收藏
- 作者：Zhang, Chen-Sheng Zhang, Zhi-Jun Li, Chun-Xiu Yu, Hui-Lei Zheng, Gao-Wei Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2012
- 关键词：Nitrilase Labrenzia aggregate Enantioselective hydrolysis (R)-o-Chloromandelic acid Biphasic system
- Enzymatic Production of l-Menthol by a High Substrate Concentration Tolerable Esterase from Newly Isolated Bacillus subtilis ECU0554被引量：48收藏
- 作者：Zheng, Gao-Wei Yu, Hui-Lei Zhang, Jian-Dong Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：ADVANCED SYNTHESIS & CATALYSIS 2009
- 关键词：Bacillus subtilis esterase enzyme catalysis high substrate concentration tolerance hydrolysis l-menthol dl-menthyl acetate
- Rational Engineering of Formate Dehydrogenase Substrate/Cofactor Affinity for Better Performance in NADPH Regeneration被引量：46收藏
- 作者：Jiang, He-Wen Chen, Qi Pan, Jiang Zheng, Gao-Wei Xu, Jian-He
- 机构：East China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2020
- 关键词：Binding affinity Burkholderia stabilis Formate dehydrogenase NADPH regeneration Rational engineering
- Development of an engineered thermostable amine dehydrogenase for the synthesis of structurally diverse chiral amines被引量：44收藏
- 作者：Liu, Lei Wang, Dong-Hao Chen, Fei-Fei Zhang, Zhi-Jun Chen, Qi Xu, Jian-He Wang, Zhi-Long Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol;Shanghai Jiao Tong Univ
- 来源：CATALYSIS SCIENCE & TECHNOLOGY 2020
- 关键词：Ammonia - Amino acids - Amines
- Asymmetric Reductive Amination of Structurally Diverse Ketones with Ammonia Using a Spectrum-Extended Amine Dehydrogenase被引量：41收藏
- 作者：Wang, Dong-Hao Chen, Qi Yin, Sai-Nan Ding, Xu-Wei Zheng, Yu-Cong Zhang, Zhi Zhang, Yu-Hui Chen, Fei-Fei Xu, Jian-He Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：ACS CATALYSIS 2021
- 关键词：< em > reductive amination < em > < em > amine dehydrogenase < em > < em > protein engineering < em > < em > substrate scope < em > < em > chiral amine < em >
- Efficient production of succinic acid in engineered Escherichia coli strains controlled by anaerobically-induced nirB promoter using sweet potato waste hydrolysate被引量：36收藏
- 作者：Huang, Meihua Cheng, Jie Chen, Peng Zheng, Gaowei Wang, Dan Hu, Yuanliang
- 机构：Chongqing Univ;East China Univ Sci & Technol;Hubei Normal Univ
- 来源：JOURNAL OF ENVIRONMENTAL MANAGEMENT 2019
- 关键词：Anaerobically-induced nirB promoter Sweet potato waste Succinic acid Engineered Escherichia coil
- Stereocomplementary Synthesis of Pharmaceutically Relevant Chiral 2-Aryl-Substituted Pyrrolidines Using Imine Reductases被引量：35收藏
- 作者：Zhang, Yu-Hui Chen, Fei-Fei Li, Bo-Bo Zhou, Xin-Yi Chen, Qi Xu, Jian-He Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol
- 来源：ORGANIC LETTERS 2020
- Evolution of Glucose Dehydrogenase for Cofactor Regeneration in Bioredox Processes with Denaturing Agents被引量：35收藏
- 作者：Qian, Wen-Zhuo Ou, Ling Li, Chun-Xiu Pan, Jiang Xu, Jian-He Chen, Qi Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol
- 来源：CHEMBIOCHEM 2020
- 关键词：biocatalysis chemical stability cofactor regeneration directed evolution glucose dehydrogenase
- Highly efficient bioreduction of 2-hydroxyacetophenone to (S)- and (R)-1-phenyl-1,2-ethanediol by two substrate tolerance carbonyl reductases with cofactor regeneration被引量：32收藏
- 作者：Cui, Zhi-Mei Zhang, Jian-Dong Fan, Xiao-Jun Zheng, Gao-Wei Chang, Hong-Hong Wei, Wen-Long
- 机构：Taiyuan Univ Technol;East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2017
- 关键词：Highly enantioselective Carbonyl reductase 2-Hydroxyacetophenone Chiral 1-phenyl-1,2-ethanediol Cofactor regeneration
- Reductive Amination of Biobased Levulinic Acid to Unnatural Chiral γ-Amino Acid Using an Engineered Amine Dehydrogenase被引量：32收藏
- 作者：Cai, Rui-Feng Liu, Lei Chen, Fei-Fei Li, Aitao Xu, Jian-He Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol;Hubei Univ;East China Univ Sci & Technol
- 来源：ACS SUSTAINABLE CHEMISTRY & ENGINEERING 2020
- 关键词：biomass reductive amination amine dehydrogenase directed evolution unnatural gamma-amino acid (S)-4-aminopentanoic acid
- De Novo Multienzyme Synthetic Pathways for Lactic Acid Production被引量：31收藏
- 作者：Ding, Xu-Wei Rong, Jing Pan, Ze-Peng Zhu, Xin-Xin Zhu, Zhen-Yu Chen, Qi Zhang, Zhi-Jun Xu, Jian-He Li, Chun-Xiu Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol
- 来源：ACS CATALYSIS 2024
- 关键词：biocatalysis C1 compound lacticacid multienzyme cascade protein engineering
- An efficient bioprocess for enzymatic production of L-menthol with high ratio of substrate to catalyst using whole cells of recombinant E. coli被引量：31收藏
- 作者：Zheng, Gao-Wei Pan, Jiang Yu, Hui-Lei Ngo-Thi, Minh-Thu Li, Chun-Xiu Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2010
- 关键词：Whole-cell biocatalysis High ratio of substrate to catalyst High substrate loading Esterase L-Menthol
- Engineering of a novel carbonyl reductase with coenzyme regeneration in E. coli for efficient biosynthesis of enantiopure chiral alcohols被引量：29收藏
- 作者：Wei, Ping Gao, Jia-Xin Zheng, Gao-Wei Wu, Hong Zong, Min-Hua Lou, Wen-Yong
- 机构：S China Univ Technol;E China Univ Sci & Technol;S China Univ Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2016
- 关键词：Asymmetric reduction Whole-cell biocatalysis Acetobacter sp GAcCR (S)-CHBE Chiral alcohols
- A Novel (R)-Imine Reductase from Paenibacillus lactis for Asymmetric Reduction of 3H-Indoles被引量：29收藏
- 作者：Li, Hao Zhang, Guang-Xiang Li, Liu-Mei Ou, Yu-Shi Wang, Ming-Yang Li, Chun-Xiu Zheng, Gao-Wei Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：CHEMCATCHEM 2016
- 关键词：asymmetric reduction biocatalysis imine reductase indoles indolines
- Bioamination of alkane with ammonium by an artificially designed multienzyme cascade被引量：28收藏
- 作者：Yu, Hui-Lei Li, Tuo Chen, Fei-Fei Luo, Xiao-Jing Li, Aitao Yang, Chao Zheng, Gao-Wei Xu, Jian-He
- 机构：East China Univ Sci & Technol;Hubei Univ;Nankai Univ;Nankai Univ
- 来源：METABOLIC ENGINEERING 2018
- 关键词：Systems biocatalysis Cascade reaction C-H amination Cyclohexylamine
- Engineered Imine Reductase for Larotrectinib Intermediate Manufacture被引量：28收藏
- 作者：Chen, Qi Li, Bo-Bo Zhang, Lilan Chen, Xin-Ru Zhu, Xin-Xin Chen, Fei-Fei Shi, Min Chen, Chun-Chi Yang, Yu Guo, Rey-Ting Liu, Weidong Xu, Jian-He Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol;Hubei Univ;Chinese Acad Sci
- 来源：ACS CATALYSIS 2022
- 关键词：asymmetric reduction biocatalysis directed evolution imine reductase larotrectinib
