成果/Result
- An improved 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay for evaluating the viability of Escherichia coli cells被引量：181收藏
- 作者：Wang, Hengwei Cheng, Hairong Wang, Fengqing Wei, Dongzhi Wang, Xuedong
- 机构：E China Univ Sci & Technol;Shanghai Jiao Tong Univ
- 来源：JOURNAL OF MICROBIOLOGICAL METHODS 2010
- 关键词：MTT reduction Formazan E. coli Viability
- Mixed bacteria-loaded biochar for the immobilization of arsenic, lead, and cadmium in a polluted soil system: Effects and mechanisms被引量：97收藏
- 作者：Ji, Xiaowen Wan, Jiang Wang, Xuedong Peng, Cheng Wang, Gehui Liang, Weiyu Zhang, Wei
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：SCIENCE OF THE TOTAL ENVIRONMENT 2022
- 关键词：Biochar Soil Heavy metals Mixed bacteria-loaded biochar Mechanisms
- Strategy for the improvement of prodigiosin production by a Serratia marcescens mutant through fed-batch fermentation被引量：45收藏
- 作者：Tao, JL Wang, XD Shen, YL Wei, DZ
- 机构：E China Univ Sci & Technol
- 来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 2005
- 关键词：feeding strategy mutation prodigiosin Serratia marcescens ultra violet
- development of an adsorption procedure for the direct separation and purification of prodigiosin from culture broth被引量：42收藏
- 作者：Wang, XD Tao, JL Wei, DZ Shen, YL Tong, WY
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 2004
- 关键词：adsorption resin chromatography fermentation in situ separation prodigiosin Serratia marcescens
- Immobilization of Ni (II) at three levels of contaminated soil by rhamnolipids modified nano zero valent iron (RL@nZVI): Effects and mechanisms被引量：40收藏
- 作者：Sang, Li Wang, Gehui Liu, Lin Bian, Hao Jiang, Lingling Wang, Huadong Zhang, Yinjie Zhang, Wei Peng, Cheng Wang, Xuedong
- 机构：East China Univ Sci & Technol;Shanghai Inst Pollut Control & Ecol Secur;East China Univ Sci & Technol
- 来源：CHEMOSPHERE 2021
- 关键词：Ni-contaminated soil Rhamnolipid nZVI Mechanisms
- Oxygen vectors used for S-adenosylmethionine production in recombinant Pichia pastoris with sorbitol as supplemental carbon source被引量：29收藏
- 作者：Zhang, Jian-Guo Wang, Xue-Dong Zhang, Ji-Ning Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOSCIENCE AND BIOENGINEERING 2008
- 关键词：S-adenosylmethionine fermentation oxygen vectors Pichia pastoris supplemental carbon source
- Enhanced Steroid Metabolites Production by Resting Cell Phytosterol Bioconversion被引量：24收藏
- 作者：Gao, X. -Q. Feng, J. -X. Wang, X. -D. Hua, Q. Wei, D. -Z.
- 机构：E China Univ Sci & Technol
- 来源：CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY 2015
- 关键词：phytosterol steroid metabolites resting cell bioconversion hydroxypropyl-beta-cyclodextrin optimization
- Light-inducible genetic engineering and control of non-homologous end-joining in industrial eukaryotic microorganisms: LML 3.0 and OFN 1.0被引量：24收藏
- 作者：Zhang, Lei Zhao, Xihua Zhang, Guoxiu Zhang, Jiajia Wang, Xuedong Zhang, Suping Wang, Wei Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Jiangxi Normal Univ;E China Univ Sci & Technol
- 来源：SCIENTIFIC REPORTS 2016
- Protein Engineering of a Nitrilase from Burkholderia cenocepacia J2315 for Efficient and Enantioselective Production of (R)-o-Chloromandelic Acid被引量：23收藏
- 作者：Wang, Hualei Gao, Wenyuan Sun, Huihui Chen, Lifeng Zhang, Lujia Wang, Xuedong Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2015
- 关键词：Hydrolysis - Genetic engineering - Biochemical engineering - Mutagenesis - Proteins
- A new fermentation strategy for S-adenosylmethionine production in recombinant Pichia pastoris被引量：23收藏
- 作者：Zhang, Jianguo Wang, Xuedong Su, Erzheng Fang, Guochen Ren, Yuhong Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：BIOCHEMICAL ENGINEERING JOURNAL 2008
- 关键词：high cell density fermentation methanol consumption rate S-adenosylmethionine Pichia postoris
- Effect of organic cosolvent on kinetic resolution of tert-leucine by penicillin G acylase from Kluyvera citrophila被引量：22收藏
- 作者：Liu, SL Wei, DZ Song, QX Zhang, YW Wang, XD
- 机构：E China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2006
- 关键词：penicillin G acylase resolution organic cosolvent tert-leucine
- Investigation of factors affecting biotransformation of phytosterols to 9-hydroxyandrost-4-ene-3,-17-dione based on the HP-β-CD-resting cells reaction system被引量：21收藏
- 作者：Gao, Xingqiang Feng, Jianxun Hua, Qiang Wei, Dongzhi Wang, Xuedong
- 机构：E China Univ Sci & Technol
- 来源：BIOCATALYSIS AND BIOTRANSFORMATION 2014
- 关键词：Biotransformation hydroxypropyl-beta-cyclodextrin Mycobacterium phytosterols rate limitation
- High purity recombinant human growth hormone (rhGH) expression in Escherichia coli under phoA promoter被引量：20收藏
- 作者：Song, Hao Jiang, Jingxin Wang, Xuedong Zhang, Jianguo
- 机构：East China Univ Sci & Technol;Univ Shanghai Sci & Technol
- 来源：BIOENGINEERED 2017
- 关键词：alkaline phosphatase Escherichia coli fed-batch fermentation strategy rhGH variant
- Mixed bacteria passivation for the remediation of arsenic, lead, and cadmium: Medium optimization and mechanisms被引量：20收藏
- 作者：Peng, Cheng Zhao, Xuan Ji, Xiaowen Wu, Jinhong Liang, Weiyu Song, Huihui Zhang, Wei Wang, Xuedong
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Shanghai Yaxin Urban Construction Co Ltd
- 来源：PROCESS SAFETY AND ENVIRONMENTAL PROTECTION 2023
- 关键词：Mixed bacteria Bacillus Optimization Heavy metal Bioremediation
- Reducing by-product formation in L-lactic acid fermentation by Rhizopus oryzae被引量：17收藏
- 作者：Wang, XD Sun, LJ Wei, DZ Wang, RM
- 机构：E China Univ Sci & Technol;Inst Shandong Light Ind
- 来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2005
- 关键词：fermentation fumaric acid L-lactic acid phosphate Rhizopus oryzae
- Preparation of optically pure tert-leucine by penicillin G acylase-catalyzed resolution被引量：17收藏
- 作者：Liu, SL Song, QX Wei, DZ Zhang, YW Wang, XD
- 机构：E China Univ Sci & Technol
- 来源：PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY 2006
- 关键词：hydrolysis Kluyvera citrophila penicillin G acylase preparation resolution tert-leucine
- A novel nitrilase from Rhodobacter sphaeroides LHS-305: cloning, heterologous expression and biochemical characterization被引量：17收藏
- 作者：Wang, Hualei Li, Guinan Li, Mingyang Wei, Dongzhi Wang, Xuedong
- 机构：E China Univ Sci & Technol
- 来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 2014
- 关键词：Nitrilase Rhodobacter sphaeroides 3-Cyanopyridine Substrate specificity Regioselectivity
- Enhancing yield of S-adenosylmethionine in Pichia pastoris by controlling NH4+ concentration被引量：15收藏
- 作者：Zhang, Jianguo Wang, Xuedong Zheng, Yu Fang, Guochen Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2008
- 关键词：S-adenosylmethionine fed-batch fermentation NH4+ concentration Pichia pastoris
- A light-switchable bidirectional expression system in filamentous fungus Trichoderma reesei被引量：15收藏
- 作者：Zhang, Guoxiu Liu, Pei Wei, Wei Wang, Xuedong Wei, Dongzhi Wang, Wei
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2016
- 关键词：Trichoderma reesei Light-switchable system Heterologous protein Simultaneous expression Cellulase-free background
- Influence of temperature on nucleus degradation of 4-androstene-3, 17-dione in phytosterol biotransformation by Mycobacterium sp.被引量：14收藏
- 作者：Xu, X. W. Gao, X. Q. Feng, J. X. Wang, X. D. Wei, D. Z.
- 机构：E China Univ Sci & Technol
- 来源：LETTERS IN APPLIED MICROBIOLOGY 2015
- 关键词：3-ketosteroid-9-hydroxylase 3-ketosteroid-(1)-dehydrogenase AD and or ADD production biodegradation Mycobacterium phytosterol temperature effect
- A New Nitrilase-Producing Strain Named Rhodobacter sphaeroides LHS-305: Biocatalytic Characterization and Substrate Specificity被引量：13收藏
- 作者：Yang, Chunsheng Wang, Xuedong Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2011
- 关键词：Rhodobacter sphaeroides Nitrilase 3-Cyanopyidine Nicotinic acid Biocatalyst
- Enhancing production and purity of 9-OH-AD from phytosterols by balancing metabolic flux of the side-chain degradation and 9-position hydroxylation in Mycobacterium neoaurum被引量：12收藏
- 作者：Zhu, Xiaomei Wang, Xiao Zhang, Jian Wang, Xuedong
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY JOURNAL 2024
- 关键词：9 alpha-hydroxyandrost-4-ene-3,17-dione Hsd4A KshA1 metabolic engineering phytosterols biotransformation
- Template-based modeling of a psychrophilic lipase: Conformational changes, novel structural features and its application in predicting the enantioselectivity of lipase catalyzed transesterification of secondary alcohols被引量：12收藏
- 作者：Xu, Tao Gao, Bei Zhang, Lujia Lin, Jingpin Wang, Xuedong Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS 2010
- 关键词：Molecular modeling Lipase Molecular dynamics Enantioselectivity
- Two-Step Bioprocess for Reducing Nucleus degradation in Phytosterol Bioconversion by Mycobacterium neoaurum NwIB-R10hsd4A被引量：12收藏
- 作者：Wang, Xuedong Hua, Chaolong Xu, Xinwei Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2019
- 关键词：3-Ketosteroid-(1)-dehydrogenase Mycobacterium neoaurum Nucleus degradation Two-step bioprocess 4-Androstene-3 17-dione
- Enhancement of riboflavin production in Bacillus subtilis via in vitro and in vivo metabolic engineering of pentose phosphate pathway被引量：8收藏
- 作者：Zhang, Mengxue Zhao, Xingcong Chen, Xi Li, Mingyue Wang, Xuedong
- 机构：East China Univ Sci & Technol;Univ Calif Davis
- 来源：BIOTECHNOLOGY LETTERS 2021
- 关键词：Bacillus subtilis Carbon metabolism flux Pentose phosphate pathway Riboflavin Sodium gluconate
- Bioprospecting metagenomics of a microbial community on cotton degradation: Mining for new glycoside hydrolases被引量：8收藏
- 作者：Zhang, Guoxiu Liu, Pei Zhang, Lei Wei, Wei Wang, Xuedong Wei, Dongzhi Wang, Wei
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2016
- 关键词：Glycoside hydrolase Microbial community Metagenomics Crude cotton Expression
- development of a large scale process for the conversion of polysialogangliosides to monosialotetrahexosylganglioside with a novel strain of Brevibacterium casei producing sialidase被引量：7收藏
- 作者：Peng, Yan-Feng Wang, Xue-Dong Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2007
- 关键词：bioconversion Brevibacterium casei gangliosides monosialotetrahexosylganlioside sialidase
- Nitrate Metabolism decreases the Steroidal Alcohol Byproduct Compared with Ammonium in Biotransformation of Phytosterol to Androstenedione by Mycobacterium neoaurum被引量：7收藏
- 作者：Wang, Xuedong Chen, Rui Wu, Yuyang Wang, Da Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2020
- 关键词：Bioconversion Metabolic redistribution Nitrate Phytosterol Redox level
- development of an efficient iterative genome editing method in Bacillus subtilis using the CRISPR-AsCpf1 system被引量：7收藏
- 作者：Zhao, Xingcong Chen, Xi Xue, Yanbing Wang, Xuedong
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BASIC MICROBIOLOGY 2022
- 关键词：Bacillus subtilis CRISPR-AsCpf1 hyaluronic acid iterative genome editing
- Identification and in situ removal of an inhibitory intermediate to develop an efficient phytosterol bioconversion process using a cyclodextrin-resting cell system被引量：7收藏
- 作者：Wang, Da Zhang, Jian Cao, Dan-Dan Wang, Xuedong Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：RSC ADVANCES 2021
- 关键词：Cyclodextrins - Metabolites - Production efficiency - Metabolism - Alcohols - Bioconversion
