- Development of a fed-batch process for the production of anticancer drug TATm-survivin(T34A) in Escherichia coli被引量：40收藏
- 作者：Zhang, Haiyi Zheng, Yu Liu, Qinghai Tao, Xinyi Zheng, Wenyun Ma, Xingyuan Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Biochemical Engineering Journal 2009
- 关键词：Batch cell culture - Controlled drug delivery - Recombinant proteins - Batch data processing - Gene expression
- 质疑
- Metagenomic sequencing reveals microbial gene catalogue of phosphinothricin-utilized soils in South China被引量：29收藏
- 作者：Lou, Jiali Liu, Min Gu, Jiali Liu, Qinghai Zhao, Li Ma, Yushu Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Huzhou Univ
- 来源：GENE 2019
- 关键词：Phosphinothricin N-acetyltransferase Metagenomics Antibiotic resistance genes Virulence factors Microbial community
- 质疑
- Enhancing Alcohol Dehydrogenase Activity for the Efficient Synthesis of (S)-2-Chloro-1-(2,4-Dichlorophenyl)Ethanol Using Computer-Aided Combinational Mutagenesis被引量：27收藏
- 作者：Ye, Wenjie Xie, Jingwen Gao, Weijie Meng, Yifang Liu, Qinghai Wang, Hualei Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering; Shanghai ZJ Bio-Tech Co.; State Key Laboratory of Bioreactor Engineering
- 来源：SSRN 2024
- 关键词：Enzyme activity - Synthesis (chemical)
- 质疑
- Efficient asymmetric synthesis of chiral alcohols using high 2-propanol tolerance alcohol dehydrogenase SmADH2 via an environmentally friendly TBCR system被引量：26收藏
- 作者：Yang, Zeyu Fu, Hengwei Ye, Wenjie Xie, Youyu Liu, Qinghai Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：CATALYSIS SCIENCE & TECHNOLOGY 2020
- 关键词：Genes - Acetone - Flow of gases - Catalyst activity - Molecular dynamics - Cloning
- 质疑
- Efficient Asymmetric Synthesis of Ethyl (S)-4-Chloro-3-hydroxybutyrate Using Alcohol Dehydrogenase SmADH31 with High Tolerance of Substrate and Product in a Monophasic Aqueous System被引量：25收藏
- 作者：Yang, Zeyu Ye, Wenjie Xie, Youyu Liu, Qinghai Chen, Rong Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Hangzhou Normal Univ
- 来源：ORGANIC PROCESS RESEARCH & DEVELOPMENT 2020
- 关键词：asymmetric synthesis ethyl (S)-4-chloro-3-hydroxybutanoate alcohol dehydrogenases high substrate and product tolerance monophasic aqueous system
- 质疑
- Application of comparative proteome analysis to reveal influence of cultivation conditions on asymmetric bioreduction of β-keto ester by Saccharomyces cerevisiae被引量：24收藏
- 作者：Lin, Jinping Liu, Qinghai Su, Erzheng Wei, Dongzhi Yang, Shengli
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied Microbiology and Biotechnology 2008
- 关键词：Batch cell culture - Cultivation - Esters - Metabolism - Cells - Enzymes
- 质疑
- Integrated Transcriptome and Proteome Studies Reveal the Underlying Mechanisms for Sterol Catabolism and Steroid Production in Mycobacterium neoaurum被引量：24收藏
- 作者：Liu, Min Xiong, Liang-Bin Tao, Xinyi Liu, Qing-Hai Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2018
- 关键词：sterol catabolism transcriptome proteome Mycobacterium
- 质疑
- High-level expression, purification and pro-apoptosis activity of HIV-TAT-survivin (T34A) mutant to cancer cells in vitro被引量：23收藏
- 作者：Ma, Xingyuan Zheng, Wenyun Wei, Dongzhi Ma, Yushu Wang, Tianwen Wang, Jinzhi Liu, Qinghai Yang, Shengli
- 机构：E China Univ Sci & Technol;Shenyang Pharmaceut Univ;Chinese Acad Sci
- 来源：JOURNAL OF BIOTECHNOLOGY 2006
- 关键词：TAT-survivin (T34A) mutant high-level expression and purification pro-apoptosis
- 质疑
- Large scale preparation of recombinant human parathyroid hormone 1-84 from Escherichia coli被引量：19收藏
- 作者：Liu, Qinghai Lin, Jinping Liu, Meiyun Tao, Xinyi Wei, Dongzhi Ma, Xingyuan Yang, Shengli
- 机构：E China Univ Sci & Technol;Chinese Acad Sci
- 来源：PROTEIN EXPRESSION AND PURIFICATION 2007
- 关键词：rhPTH(1-84) fusion protein chromatography enterokinase adenylate cyclase large scale preparation Escherichia coli
- 质疑
- Production of sesquiterpene patchoulol in mitochondrion-engineered Saccharomyces cerevisiae被引量：16收藏
- 作者：Tao, Xin-Yi Lin, Yang-Chen Wang, Feng-Qing Liu, Qing-Hai Ma, Yu-Shu Liu, Min Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2022
- 关键词：Patchoulol Mitochondria compartmentation S cerevisiae Metabolic engineering Sesquiterpene
- 质疑
- Metabolic Adaptation of Mycobacterium neoaurum ATCC 25795 in the Catabolism of Sterols for Producing Important Steroid Intermediates被引量：15收藏
- 作者：Liu, Min Xiong, Liang-Bin Tao, Xinyi Liu, Qing-Hai Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2018
- 关键词：sterol catabolism mycobacteria cell envelope steroids efflux pumps
- 质疑
- Efficient Biocatalytic Synthesis of (R)-2-Chloro-1-(3,4-difluorophenyl)ethanol by the Short-Chain Dehydrogenase PpKR8 from Paraburkholderia phymatum STM815被引量：14收藏
- 作者：He, Ling Ye, Wenjie Xie, Youyu Liu, Qinghai Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：ORGANIC PROCESS RESEARCH & DEVELOPMENT 2022
- 关键词：(R)-2-chloro-1-(3,4-difluorophenyl)ethanol alcohol dehydrogenase asymmetric reduction coexpression
- 质疑
- Comparison of the expression of phospholipase D from Streptomyces halstedii in different hosts and its over-expression in Streptomyces lividans被引量：14收藏
- 作者：Tao, Xinyi Zhao, Ming Zhang, Ying Liu, Min Liu, Qinghai Wang, Wei Wang, Feng-Qing Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：FEMS MICROBIOLOGY LETTERS 2019
- 关键词：phospholipase D Streptomyces lividans Pichia pastoris cytotoxic dual-promoter
- 质疑
- Characterization of a novel nitrilase, BGC4, from Paraburkholderia graminis showing wide-spectrum substrate specificity, a potential versatile biocatalyst for the degradation of nitriles被引量：13收藏
- 作者：Fan, Haiyang Chen, Lifeng Sun, Huihui Wang, Hualei Liu, Qinghai Ren, Yuhong Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Chinese Acad Fishery Sci Qingdao
- 来源：BIOTECHNOLOGY LETTERS 2017
- 关键词：Biodegradation Nitrilase Paraburkholderia graminis Wide-spectrum substrate specificity
- 质疑
- Development of a fed-batch process for the production of anticancer drug TATm-survivin(T34A) in Escherichia coli被引量：10收藏
- 作者：Zhang, Haiyi Zheng, Yu Liu, Qinghai Tao, Xinyi Zheng, Wenyun Ma, Xingyuan Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：BIOCHEMICAL ENGINEERING JOURNAL 2009
- 关键词：TAT(m)-survivin(T34A) Escherichia coli Fed-batch culture Optimization Acetic acid Bioprocess design
- 质疑
- The identification and application of a robust ω-transaminase with high tolerance towards substrates and isopropylamine from a directed soil metagenome被引量：9收藏
- 作者：Xie, Youyu Wang, Jiguo Yang, Lin Wang, Wei Liu, Qinghai Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：CATALYSIS SCIENCE & TECHNOLOGY 2022
- 关键词：Amines - Efficiency - Ketones - Reaction kinetics - Molecular dynamics
- 质疑
- Development of nitrilase-mediated process for phenylacetic acid production from phenylacetonitrile被引量：8收藏
- 作者：Fan, Haiyang Chen, Lifeng Sun, Huihui Wang, Hualei Liu, Qinghai Ren, Yuhong Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Chinese Acad Fishery Sci
- 来源：CHEMICAL PAPERS 2017
- 关键词：Biotransformation Fed-batch reaction Fermentation Nitrilase Phenylacetic acid
- 质疑
- 重组HIV-TATm-Survivin(T34A)蛋白制备及其促癌细胞凋亡活性研究被引量：4收藏
- 作者：郑文云 马兴元 魏东芝 王锦之 刘清海 马昱澍 杨胜利
- 机构：华东理工大学生物反应器工程国家重点实验室生物化学研究所
- 来源：《生物工程学报》 2006
- 关键词：重组HIV-TATm-Survivin(T34A)突变体 表达纯化 促凋亡活性 B-Cap-37和SW1990癌细胞系
- 摘要：从人乳腺癌细胞系B-Cap-37中克隆出Survivin的cDNA,并对其中编码34位Thr的密码子定点突变为Ala的密码子后,经一系列基因操作方法将天然HIV-TAT转导肽的突变体基因HIV-TATm引入Survivi...
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- Semirational Engineering of a Distal Loop Region to Enhance the Catalytic Activity and Stability of Leucine Dehydrogenase被引量：4收藏
- 作者：Li, Rui Xu, Minglu Xu, Meng Zhou, Yiquan Wen, Tao Liu, Qinghai Yi, Dong Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;China State Inst Pharmaceut Ind
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2025
- 关键词：
l -phenylglycine distant modulation loop engineering benzoylformicacid enantiomericexcess triple-point mutation formate dehydrogenase molecular dynamics simulations - 质疑
- Inside-Out Rational Design of Ornithine Cyclodeaminase RlOCD from Rhizobium leguminosarum by a Multiregion Synergy Strategy for Efficient Synthesis of l-Pipecolic Acid被引量：3收藏
- 作者：Gao, Weijie Jing, Zijian Meng, Yifang Liu, Qinghai Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2024
- 关键词：
l -pipecolic acid ornithine cyclodeaminase rational design - 质疑
- Preparation and purification of novel phosphatidyl prodrug and performance modulation of phosphatidyl nanoprodrug被引量：2收藏
- 作者：Niu, Rui Zhang, PeiLei Wang, Feng-Qing Liu, Min Liu, QingHai Jia, Ning Yang, ShengLi Tao, XinYi Wei, DongZhi
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2019
- 关键词：Phosphatidyl prodrug Purification Gradient elution Silica gel column chromatography PEGylation RGD modification
- 质疑
- Substrate-Cofactor Synergistic Reorientation and Substrate Tunnel Engineering of SspLCD for Efficient l-Pipecolic Acid Biosynthesis被引量：2收藏
- 作者：Gao, Weijie Huang, Ziyan Li, Yanjun Sun, Shuya Liu, Qinghai Wang, Hualei Chen, Rong Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Hangzhou Normal Univ
- 来源：ORGANIC LETTERS 2025
- 质疑
- 变形杆菌属脂肪酶LipK107的分离纯化、结晶及初步晶体学分析被引量：2收藏
- 作者：戚宝杰 刘清海 谢静莉 魏东芝
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《食品工业科技》 2012
- 关键词：脂肪酶LipK107 纯化 结晶 X-射线衍射
- 摘要：经过异丙基-β-D-硫代吡喃半乳糖苷(IPTG)诱导,重组大肠杆菌高效表达出带有组氨酸标签的可溶性脂肪酶LipK107。通过亲和层析和凝胶层析两步法纯化,得到电泳纯的脂肪酶样品。在获得的纯酶基础上,进行了圆二色谱实验,以...
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- Enhancing Alcohol Dehydrogenase Activity for the Efficient Synthesis of (S)-2-Chloro-1-(2,4-dichlorophenyl)ethanol Using Computer-Aided Combinational Mutagenesis被引量：1收藏
- 作者：Ye, Wenjie Xie, Jingwen Gao, Weijie Meng, Yifang Liu, Qinghai Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai ZJ Biotech Co Ltd
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2025
- 关键词：alcohol dehydrogenase enzyme engineering virtual saturation mutagenesis combinational mutagenesis (S)-2-chloro-1-(2,4-dichlorophenyl)ethanol
- 质疑
- 末端连接ω-氨基酸的聚乙二醇修饰剂的制备被引量：1收藏
- 作者：熊玉春 刘清海 何明磊 魏东芝
- 机构：华东理工大学生物反应器工程国家重点实验室鲁华生物技术研究所;大连民族学院生命科学学院
- 来源：《华东理工大学学报（自然科学版）》 2007
- 关键词：聚乙二醇 ω-氨基酸 聚乙二醇修饰 活化 制备
- 摘要：提出了一种新型聚乙二醇(PEG)修饰剂的合成方法。首先以两种ω-氨基酸和单甲氧基PEG为主要原料,经连接、纯化和水解等步骤,得到末端以酰胺键连接ω-氨基酸的PEG酸,再将得到的PEG酸用N-羟基琥珀酰亚胺(NHS)进行活...
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- Rational engineering of Corynebacterium glutamicum for L-Glutamine biosynthesis被引量：0收藏
- 作者：Lin, Ying-Tong Chai, Meng Liu, Qing-Hai Ma, Yu-Shu Tao, Xin-Yi Liu, Min Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：BIOCHEMICAL ENGINEERING JOURNAL 2026
- 关键词：L-Glutamine Metabolic engineering RBS engineering SRNA interference Growth-responsive promoter
- 质疑
- Application of comparative proteome analysis to reveal influence of cultivation conditions on asymmetric bioreduction of β-keto ester by Saccharomyces cerevisiae被引量：0收藏
- 作者：Lin, Jinping Liu, Qinghai Su, Erzheng Wei, Dongzhi Yang, Shengli
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2008
- 关键词：asymmetric bioreduction Saccharomyces cerevisiae growth medium ethyl benzoylacetate proteome analysis
- 质疑
- 微生物转化在米格列醇合成中的应用被引量：0收藏
- 作者：林金萍 粟武 刘清海 高克亮 魏东芝
- 机构：华东理工大学生物反应器国家重点实验室
- 来源：《第四届中国酶工程学术交流讨论会》 2003
- 摘要：<正>1米格列醇的介绍糖尿病是最为常见的一种内分泌代谢失调引起的疾病,主要分为Ⅰ型糖尿病(胰岛素依赖性糖尿病,IDD)和Ⅱ型糖尿病(非胰岛素依赖性糖尿病,NIDD)。随着人民生活水平的提高和营养结构的改善,糖尿病作为慢性...
- 质疑
- 微生物转化在米格列醇合成中的应用被引量：0收藏
- 作者：林金萍 粟武 刘清海 高克亮 魏东芝
- 机构：华东理工大学生物反应器国家重点实验室
- 来源：《第四届中国酶工程学术交流讨论会》 2003
- 关键词：米格列醇 型糖尿病 降糖效果 微生物转化
- 摘要：1米格列醇的介绍糖尿病是最为常见的一种内分泌代谢失调引起的疾病,主要分为Ⅰ型糖尿病(胰岛素依赖性糖尿病,IDD)和Ⅱ型糖尿病(非胰岛素依赖性糖尿病,NIDD)。随着人民生活水平的提高和营养结构的改善,糖尿病作为慢性非传染...
- 质疑
- Preparation and purification of novel phosphatidyl prodrug and performance modulation of phosphatidyl nanoprodrug被引量：0收藏
- 作者：Rui Niu PeiLei Zhang Feng-Qing Wang Min Liu QingHai Liu Ning Jia ShengLi Yang XinYi Tao DongZhi Wei
- 机构：State Key Laboratory of Bioreactor Engineering
- 来源：生物资源与生物加工(英文) 2019
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