马昱澍 收藏
( 任职于 生物工程学院鲁华生物技术研究所)
生物化学与分子生物学专业：硕士生导师、微生物学专业：硕士生导师、生物工程领域 ：工程硕士生导师 男，1973年6月出生，博士，华东理工大学生物工程学院副教授,硕士生导师 学历及工作经历 1991年10月－1997年6月 俄罗斯联邦国立莫斯科大学生物系生物化学专业，获得生物化学硕士学位 1997年7月－2000年6月 俄罗斯联邦国立莫斯科大学生物系生物化学专业，获得生物化学博士学位 2000年9月－2002年9月 复旦大学遗传工程国家重点实验室博士后 2002年10月至今 华东理工大学生物工程学院副教授 ... 详细>>
- Mutant library construction in directed molecular evolution: Casting a wider net被引量：74收藏
- 作者：Wang, Tian-Wen Zhu, Hu Ma, Xing-Yuan Zhang, Ting Ma, Yu-Shu Wei, Dong-Zhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Molecular Biotechnology 2006
- 关键词：Cells - DNA - Enzymes - Molecular biology - Mutagenesis - Mutagens
- 质疑
- A novel psychrophilic lipase from Pseudomonas fluorescens with unique property in chiral resolution and biodiesel production via transesterification被引量：66收藏
- 作者：Luo, Yu Zheng, Yitao Jiang, Zhengbing Ma, Yushu Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2006
- 质疑
- High-Level Production of Sesquiterpene Patchoulol in Saccharomyces cerevisiae被引量：57收藏
- 作者：Liu, Min Lin, Yang-Chen Guo, Jiao-Jiao Du, Meng-Meng Tao, Xinyi Gao, Bei Zhao, Ming Ma, Yushu Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：ACS SYNTHETIC BIOLOGY 2021
- 关键词：patchoulol physiological status metabolic engineering Saccharomyces cerevisiae sesquiterpene cell factories
- 质疑
- Development of recombinant Escherichia coli whole-cell biocatalyst expressing a novel alkaline lipase-coding gene from Proteus sp for biodiesel production被引量：45收藏
- 作者：Gao, Bei Su, Erzheng Lin, Jinping Jiang, Zhengbing Ma, Yushu Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2009
- 关键词：LipK107 E. coli whole-cell catalyst Biodiesel Low-temperature property
- 质疑
- Mutant library construction in directed molecular evolution被引量：40收藏
- 作者：Wang, Tian-Wen Zhu, Hu Ma, Xing-Yuan Zhang, Ting Ma, Yu-Shu Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：MOLECULAR BIOTECHNOLOGY 2006
- 关键词：directed molecular evolution error-prone PCR oligonucleotide-based mutagenesis DNA shuffling mutator strain somatic hypermutation
- 质疑
- Expression and purification of antimicrobial peptide adenoregulin with C-amidated terminus in Escherichia coli被引量：37收藏
- 作者：Cao, W Zhou, YX Ma, YS Luo, QP Wei, DZ
- 机构：E China Univ Sci & Technol
- 来源：PROTEIN EXPRESSION AND PURIFICATION 2005
- 关键词：C-amidated terminus adenoregulin antimicrobial peptide expression Escherichia coli
- 质疑
- Development of recombinant Escherichia coli whole-cell biocatalyst expressing a novel alkaline lipase-coding gene from Proteus sp. for biodiesel production被引量：33收藏
- 作者：Gao, Bei Su, Erzheng Lin, Jinping Jiang, Zhengbing Ma, Yushu Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Biotechnology 2009
- 关键词：Catalysts - Cloning - Cytology - Gene encoding - Temperature - Biodiesel - Purification - Alkalinity
- 质疑
- Efficient display of active lipase LipB52 with a Pichia pastoris cell surface display system and comparison with the LipB52 displayed on Saccharomyces cerevisiae cell surface被引量：32收藏
- 作者：Jiang, Zhengbing Gao, Bei Ren, Ren Tao, Xingyi Ma, Yushu Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Hubei Univ
- 来源：BMC BIOTECHNOLOGY 2008
- 质疑
- Enhancing enzymatic activity of penicillin G acylase by coexpressing pcm gene被引量：30收藏
- 作者：Wang, Tianwen Zhu, Hu Ma, Xingyuan Fei, Zhuoya Ma, Yushu Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied Microbiology and Biotechnology 2006
- 关键词：Antibiotics - Biotechnology - Cells - Escherichia coli - Genes
- 质疑
- Metagenomic sequencing reveals microbial gene catalogue of phosphinothricin-utilized soils in South China被引量：29收藏
- 作者：Lou, Jiali Liu, Min Gu, Jiali Liu, Qinghai Zhao, Li Ma, Yushu Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Huzhou Univ
- 来源：GENE 2019
- 关键词：Phosphinothricin N-acetyltransferase Metagenomics Antibiotic resistance genes Virulence factors Microbial community
- 质疑
- Efficient display of active lipase LipB52 with a Pichia pastoris cell surface display system and comparison with the LipB52 displayed on Saccharomyces cerevisiae cell surface被引量：26收藏
- 作者：Jiang, Zhengbing Gao, Bei Ren, Ren Tao, Xingyi Ma, Yushu Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering; College of Life Science
- 来源：BMC Biotechnology 2008
- 关键词：Biocatalysts - Esters - Flocculation - Genes - Hydrolysis - Phenols - Transesterification - Yeast
- 质疑
- N,N-dimethylformamide induces cellulase production in the filamentous fungus Trichoderma reesei被引量：25收藏
- 作者：Chen, Yumeng Wu, Chuan Shen, Yaling Ma, Yushu Wei, Dongzhi Wang, Wei
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY FOR BIOFUELS 2019
- 关键词：Trichoderma reesei DMF induced Cellulase Calcium signaling Permeability Phospholipase C plc-e
- 质疑
- High-level expression, purification and pro-apoptosis activity of HIV-TAT-survivin (T34A) mutant to cancer cells in vitro被引量：23收藏
- 作者：Ma, Xingyuan Zheng, Wenyun Wei, Dongzhi Ma, Yushu Wang, Tianwen Wang, Jinzhi Liu, Qinghai Yang, Shengli
- 机构：E China Univ Sci & Technol;Shenyang Pharmaceut Univ;Chinese Acad Sci
- 来源：JOURNAL OF BIOTECHNOLOGY 2006
- 关键词：TAT-survivin (T34A) mutant high-level expression and purification pro-apoptosis
- 质疑
- Construction of a Novel Shuttle Vector for Use in Gluconobacter oxydans被引量：21收藏
- 作者：Zhang, Lin Lin, Jinping Ma, Yushu Wei, Dongzhi Sun, Ming
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol
- 来源：MOLECULAR BIOTECHNOLOGY 2010
- 关键词：Shuttle vector Gluconobacter oxydans Cryptic plasmid Expression
- 质疑
- A simple approach to mediate genome editing in the filamentous fungus Trichoderma reesei by CRISPR/Cas9-coupled in vivo gRNA transcription被引量：20收藏
- 作者：Wu, Chuan Chen, Yumeng Qiu, Yifei Niu, Xiao Zhu, Ningjian Chen, Jiehui Yao, Hong Wang, Wei Ma, Yushu
- 机构：East China Univ Sci & Technol;East China Normal Univ
- 来源：BIOTECHNOLOGY LETTERS 2020
- 关键词：Filamentous fungus Trichoderma reesei CRISPR Cas9 In vivo gRNA transcription Gene editing
- 质疑
- Expression and purification of exendin-4, a GLP-1 receptor agonist, in Escherichia coli被引量：19收藏
- 作者：Yin, XP Wei, DZ Yi, LN Tao, XY Ma, YS
- 机构：E China Univ Sci & Technol
- 来源：PROTEIN EXPRESSION AND PURIFICATION 2005
- 关键词：exendin-4 expression glucose-lowering action
- 质疑
- Analysis of the binding site of the n-terminal fragment of glucogan-like peptide-1 receptor to exendin-4被引量：19收藏
- 作者：Gao, Wei-Feng Liu, Mei-Yun Ma, Yushu
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Huadong Ligong Daxue Xuebao /Journal of East China University of Science and Technology 2008
- 质疑
- A novel psychrophilic lipase from Pseudomonas fluorescens with unique property in chiral resolution and biodiesel production via transesterification被引量：19收藏
- 作者：Luo, Yu Zheng, Yitao Jiang, Zhengbing Ma, Yushu Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied Microbiology and Biotechnology 2006
- 关键词：Affinity chromatography - Amino acids - Biodiesel - Catalyst activity - Escherichia coli - Gene expression - Lipases
- 质疑
- 新型脂肪酶LipB52催化生物柴油被引量：18收藏
- 作者：王刚 罗宇 江正兵 魏东芝 马昱澍
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2008
- 关键词：生物柴油 脂肪酶 荧光假单胞菌 转酯反应
- 摘要：一种来源于荧光假单胞菌的新型脂肪酶基因lipB52在大肠杆菌BL21(DE3)中获得表达,并且利用Ni-NTA亲和柱对脂肪酶LipB52进行纯化。采用固定化脂肪酶LipB52催化大豆油与甲醇的转酯反应生产生物柴油。实验考...
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- Expression of two old yellow enzyme homologues from Gluconobacter oxydans and identification of their citral hydrogenation abilities被引量：17收藏
- 作者：Yin, Bo Yang, Xuepeng Wei, Guodong Ma, Yushu Wei, Dongzhi
- 机构： State Key Lab of Bioreactor Engineering; College of Food and Biological Engineering
- 来源：Molecular Biotechnology 2008
- 关键词：Gene expression - Cloning - Amino acids - Hydrogenation - Purification - Enzymes
- 质疑
- Structure-guided engineering of a flavin-containing monooxygenase for the efficient production of indirubin被引量：17收藏
- 作者：Sun, Bing-Yao Sui, Hua-Lu Liu, Zi-Wei Tao, Xin-Yi Gao, Bei Zhao, Ming Ma, Yu-Shu Zhao, Jian Liu, Min Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2022
- 关键词：Flavin-containing monooxygenase Indirubin Loop region Substrate tunnel Structure-guided enzyme engineering Microbial synthesis
- 质疑
- Display of adenoregulin with a novel Pichia pastoris cell surface display system被引量：17收藏
- 作者：Ren, Ren Jiang, Zhengbing Liu, Meiyun Tao, Xinyi Ma, Yushu Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Molecular Biotechnology 2007
- 关键词：Amino acids - Cells - Confocal microscopy - Enzyme kinetics - Flocculation - Yeast
- 质疑
- Production of sesquiterpene patchoulol in mitochondrion-engineered Saccharomyces cerevisiae被引量：16收藏
- 作者：Tao, Xin-Yi Lin, Yang-Chen Wang, Feng-Qing Liu, Qing-Hai Ma, Yu-Shu Liu, Min Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2022
- 关键词：Patchoulol Mitochondria compartmentation S cerevisiae Metabolic engineering Sesquiterpene
- 质疑
- An efficient shortened genetic transformation strategy for filamentous fungus Trichoderma reesei被引量：16收藏
- 作者：Wu, Chuan Chen, Yumeng Huang, Xiaoxue Sun, Shishuai Luo, Jinnan Lu, Zhiwen Wang, Wei Ma, Yushu
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY 2019
- 关键词：G418 optimized codons Trichoderma reesei two-Agrobacterium-mediated transformation
- 质疑
- Characterisation of a thermostable catechol-2,3-dioxygenase from phenanthrene-degrading Pseudomonas sp strain ZJF08被引量：15收藏
- 作者：Zhou, Ying Wei, Jiashi Jiang, Tao Gao, Weifeng Ma, Yushu Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Fudan Univ
- 来源：ANNALS OF MICROBIOLOGY 2007
- 关键词：biodegradation catechol-2,3-dioxygenase PAH dioxygenase phenanthrene thermostability
- 质疑
- Characterization of two novel lipase genes isolated directly from environmental sample被引量：13收藏
- 作者：Jiang, Zhengbing Wang, Hongping Ma, Yushu Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2006
- 关键词：Amino acids - Characterization - Environmental impact - Enzymes - Thermal effects
- 质疑
- In vivo functional expression of an extracellular Ca2+-independent Bacillus pumilus lipase in Bacillus subtilis WB800N被引量：13收藏
- 作者：Ma, Jing Ma, Yushu Wei, Wei Wei, Dong-zhi
- 机构：E China Univ Sci & Technol
- 来源：ANNALS OF MICROBIOLOGY 2015
- 关键词：Strain screening Lipase Gene cloning Enzymatic properties Bacillus subtilis expression
- 质疑
- Phenanthrene biodegradation by an indigenous Pseudomonas sp ZJF08 with TX100 as surfactant被引量：12收藏
- 作者：Zhou, Ying Zhang, Jiangguo Su, Erzheng Wei, Guodong Ma, Yushu Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：ANNALS OF MICROBIOLOGY 2008
- 关键词：surfactant phenanthrene biodegradation Pseudomonas sp.
- 质疑
- Cloning and expression of a novel lipase gene from Pseudomonas fluorescens B52被引量：12收藏
- 作者：Jiang, ZB Zheng, YT Luo, Y Wang, G Wang, HP Ma, YS Wei, DZ
- 机构：E China Univ Sci & Technol
- 来源：MOLECULAR BIOTECHNOLOGY 2005
- 关键词：expression genome-walking lipase Pichia pastoris Pseudomonas fluorescens
- 质疑
- Structure-based stabilization of an enzyme:: The case of penicillin acylase from Alcaligenes faecalis被引量：12收藏
- 作者：Wang, TW Zhu, H Ma, XY Ma, YS Wei, DZ
- 机构：E China Univ Sci & Technol
- 来源：PROTEIN AND PEPTIDE LETTERS 2006
- 关键词：thermal stability penicillin acylase site-directed mutagenesis homology modeling double mutations
- 质疑
