王风清 收藏
( 任职于 生物工程学院)
生物化工专业：硕士生导师 生物化学与分子生物学专业：硕士生导师 2000年和2003年分别于天津科技大学获学士和硕士学位，2007年毕业于华东理工大学，获生物化工博士学位。长期致力于“微生物代谢工程”相关的研究工作，研究内容涉及：“甾体药物微生物代谢转化合成”、“草本天然产物的微生物合成”、“脂质转化及药物修饰用高值酶”等。近几年，以第一作者或通讯作者在“Metabolic engineering&rdq... 详细>>
- The yeast peroxisome: A dynamic storage depot and subcellular factory for squalene overproduction被引量：194收藏
- 作者：Liu, Guo-Song Li, Tian Zhou, Wei Jiang, Min Tao, Xin-Yi Liu, Min Zhao, Ming Ren, Yu-Hong Gao, Bei Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2020
- 关键词：Synthetic biology Saccharomyces cerevisiae Peroxisome Storage depot Subcellular factory Terpene
- 质疑
- An improved 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay for evaluating the viability of Escherichia coli cells被引量：181收藏
- 作者：Wang, Hengwei Cheng, Hairong Wang, Fengqing Wei, Dongzhi Wang, Xuedong
- 机构：E China Univ Sci & Technol;Shanghai Jiao Tong Univ
- 来源：JOURNAL OF MICROBIOLOGICAL METHODS 2010
- 关键词：MTT reduction Formazan E. coli Viability
- 质疑
- Metabolic compartmentalization in yeast mitochondria: Burden and solution for squalene overproduction被引量：115收藏
- 作者：Zhu, Zhan-Tao Du, Meng-Meng Gao, Bei Tao, Xin-Yi Zhao, Ming Ren, Yu-Hong Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2021
- 关键词：Saccharomyces cerevisiae Mitochondrial engineering Metabolic burden Squalene Dual engineering
- 质疑
- Characterization and engineering of 3-ketosteroid-Δ1-dehydrogenase and 3-ketosteroid-9α-hydroxylase in Mycobacterium neoaurum ATCC 25795 to produce 9α-hydroxy-4-androstene-3,17-dione through the catabolism of sterols被引量：99收藏
- 作者：Yao, Kang Xu, Li-Qin Wang, Feng-Qing Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2014
- 关键词：3-Ketosteroid-Delta(1)-dehydrogenase Mycobacteria Sterols 9 alpha-Hydroxy-4-androstene-3,17-dione
- 质疑
- Identification and engineering of cholesterol oxidases involved in the initial step of sterols catabolism in Mycobacterium neoaurum被引量：93收藏
- 作者：Yao, Kang Wang, Feng-Qing Zhang, Huai-Cheng Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2013
- 关键词：Cholesterol oxidase Mycobacteria Sterols Androst-4-ene-3,17-dione Androst-1,4-diene-3,17-dione
- 质疑
- Unraveling and engineering the production of 23,24-bisnorcholenic steroids in sterol metabolism被引量：80收藏
- 作者：Xu, Li-Qin Liu, Yong-Jun Yao, Kang Liu, Hao-Hao Tao, Xin-Yi Wang, Feng-Qing Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：SCIENTIFIC REPORTS 2016
- 质疑
- Metabolic Engineering of Saccharomyces cerevisiae To Overproduce Squalene被引量：71收藏
- 作者：Li, Tian Liu, Guo-Song Zhou, Wei Jiang, Min Ren, Yu-Hong Tao, Xin-Yi Liu, Min Zhao, Ming Wang, Feng-Qing Gao, Bei Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2020
- 关键词：Saccharomyces cerevisiae metabolic engineering squalene overproduction ethanol carbon source utilization
- 质疑
- Simple and Cheap CRISPR/Cas12a Biosensor Based on Plug-and-Play of DNA Aptamers for the Detection of Endocrine-Disrupting Compounds被引量：65收藏
- 作者：Wang, Feng-Qing Zhao, Yunqiu Zhu, Lin Ding, Yaxue Ji, Weiting Liu, Kun Liu, Ke Gao, Bei Tao, Xinyi Dong, Yu-Guo Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering; Key Laboratory of Biocatalysis and Intelligent Manufacturing
- 来源：SSRN 2023
- 关键词：DNA - Endocrine disrupters - Phenols
- 质疑
- Inactivation and Augmentation of the Primary 3-Ketosteroid-Δ1-Dehydrogenase in Mycobacterium neoaurum NwIB-01: Biotransformation of Soybean Phytosterols to 4-Androstene-3,17-Dione or 1,4-Androstadiene-3,17-Dione被引量：63收藏
- 作者：Wei, Wei Wang, Feng-qing Fan, Shu-yue Wei, Dong-zhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2010
- 质疑
- High-Level Production of Sesquiterpene Patchoulol in Saccharomyces cerevisiae被引量：57收藏
- 作者：Liu, Min Lin, Yang-Chen Guo, Jiao-Jiao Du, Meng-Meng Tao, Xinyi Gao, Bei Zhao, Ming Ma, Yushu Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：ACS SYNTHETIC BIOLOGY 2021
- 关键词：patchoulol physiological status metabolic engineering Saccharomyces cerevisiae sesquiterpene cell factories
- 质疑
- Significantly Enhanced Production of Patchoulol in Metabolically Engineered Saccharomyces cerevisiae被引量：53收藏
- 作者：Ma, Bin Liu, Min Li, Zhen-Hai Tao, Xinyi Wei, Dong-Zhi Wang, Feng-Qing
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2019
- 关键词：patchoulol sesquiterpene metabolic engineering Saccharomyces cerevisiae terpenoids
- 质疑
- Ammonia-containing dimethyl sulfoxide: An improved solvent for the dissolution of formazan crystals in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay被引量：51收藏
- 作者：Wang, Hengwei Wang, Fengqing Tao, Xinyi Cheng, Hairong
- 机构：Shanghai Jiao Tong Univ;E China Univ Sci & Technol
- 来源：ANALYTICAL BIOCHEMISTRY 2012
- 关键词：MTT assay Formazan Dimethyl sulfoxide Ammonia pH Viability
- 质疑
- Metabolic engineering of Pichia pastoris for the production of dammarenediol-II被引量：51收藏
- 作者：Liu, Xin-Bin Liu, Min Tao, Xin-Yi Zhang, Zhong-Xi Wang, Feng-Qing Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2015
- 关键词：Dammarenediol-II Metabolic engineering Pichia pastoris Ginsenosides 2,3-Oxidosqualene
- 质疑
- Establishment of an Efficient Expression and Regulation System in Streptomyces for Economical and High-Level Production of the Natural Blue Pigment Indigoidine被引量：46收藏
- 作者：Zhao, Ming Zhang, Xiu-Shan Xiong, Liang-Bin Liu, Kun Li, Xiang-Fei Liu, Yan Wang, Feng-Qing
- 机构： Anhui Engineering Laboratory for Industrial Microbiology Molecular Breeding; State Key Laboratory of Bioreactor Engineering; Shanghai Key Laboratory of Molecular Imaging
- 来源：Journal of Agricultural and Food Chemistry 2024
- 关键词：Amino acids - Bacteria - Condensation reactions - Metabolism
- 质疑
- New microbiological transformations of steroids by Streptomyces virginiae IBL-14被引量：46收藏
- 作者：Wang, Feng-Qing Zhang, Cheng-Gang Li, Bo Wei, Dong-Zhi Tong, Wang-Yu
- 机构： State Key Laboratory of Bioreactor Engineering; Integrated Biotechnology Laboratory
- 来源：Environmental Science and Technology 2009
- 关键词：Lipids - Metabolism - Waste treatment
- 质疑
- Impact of oxygen supply on rtPA expression in Escherichia coli BL21 (DE3): Ammonia effects被引量：45收藏
- 作者：Wang, Hengwei Wang, Fengqing Wei, Dongzhi
- 机构： Newworld Institute of Biotechnology
- 来源：Applied Microbiology and Biotechnology 2009
- 关键词：Bottles - Amino acids - Nitrogen - Cell growth - Growth kinetics - Recombinant proteins - Ammonia - Batch cell culture - Ions - Oxygen supply
- 质疑
- Enhancing the bioconversion of phytosterols to steroidal intermediates by the deficiency of kasB in the cell wall synthesis of Mycobacterium neoaurum被引量：45收藏
- 作者：Xiong, Liang-Bin Liu, Hao-Hao Zhao, Ming Liu, Yong-Jun Song, Lu Xie, Zhi-Yong Xu, Yi-Xin Wang, Feng-Qing Wei, Dong-Zhi
- 机构：Shanghai Univ Med & Hlth Sci;East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci
- 来源：MICROBIAL CELL FACTORIES 2020
- 关键词：Mycolic acids Self-enhancement conversion beta-Ketoacyl-acyl carrier protein synthase Phytosterol Steroid intermediates
- 质疑
- The MrCYP52 Cytochrome P450 Monoxygenase Gene of Metarhizium robertsii Is Important for Utilizing Insect Epicuticular Hydrocarbons被引量：41收藏
- 作者：Lin, Liangcai Fang, Weiguo Liao, Xinggang Wang, Fengqing Wei, Dongzhi Leger, Raymond J. St.
- 机构：E China Univ Sci & Technol;Univ Maryland
- 来源：PLOS ONE 2011
- 质疑
- Identification and engineering of cholesterol oxidases involved in the initial step of sterols catabolism in Mycobacterium neoaurum被引量：39收藏
- 作者：Yao, Kang Wang, Feng-Qing Zhang, Huai-Cheng Wei, Dong-Zhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Metabolic Engineering 2013
- 关键词：Lipids - Alcohols
- 质疑
- Improving the production of 22-hydroxy-23,24-bisnorchol-4-ene-3-one from sterols in Mycobacterium neoaurum by increasing cell permeability and modifying multiple genes被引量：38收藏
- 作者：Xiong, Liang-Bin Liu, Hao-Hao Xu, Li-Qin Sun, Wan-Ju Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：MICROBIAL CELL FACTORIES 2017
- 关键词：Mycobacterium 22-Hydroxy-23,24-bisnorchol-4-ene-3-one (4-HBC) mmpL3 choM cyp125 fadA5 Sterol catabolism
- 质疑
- Enhancing Biosynthesis of a Ginsenoside Precursor by Self-Assembly of Two Key Enzymes in Pichia pastoris被引量：36收藏
- 作者：Zhao, Chengcheng Gao, Xin Liu, Xinbin Wang, Yong Yang, Shengli Wang, Fengqing Ren, Yuhong
- 机构：E China Univ Sci & Technol;Chinese Acad Sci
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2016
- 关键词：ginsenosides self-assembly Pichia pastoris dammarenediol-II
- 质疑
- Engineered 3-Ketosteroid 9α-Hydroxylases in Mycobacterium neoaurum: an Efficient Platform for Production of Steroid Drugs被引量：36收藏
- 作者：Liu, Hao-Hao Xu, Li-Qin Yao, Kang Xiong, Liang-Bin Tao, Xin-Yi Liu, Min Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2018
- 关键词：3-ketosteroid-9 alpha-hydroxylase androst-1,4-diene-3,17-dione (ADD) 9 alpha-hydroxy-4-androstene-3,17-dione (9-OHAD) mycobacterium Mn25795 sterols 9 alpha-hydroxy-4-androstene-3,17-dione androst-1,4-diene-3,17-dione sterol
- 质疑
- A comparative study of β-1, 4-endoglucanase (possessing β-1, 4-exoglucanase activity) from Bacillus subtilis LH expressed in Pichia pastoris GS115 and Escherichia coli Rosetta (DE3)被引量：35收藏
- 作者：Zhao, Xi-Hua Wang, Wei Wang, Feng-Qing Wei, Dong-Zhi
- 机构： State Key Lab of Bioreactor Engineering; College of Life Science
- 来源：Bioresource Technology 2012
- 关键词：Yeast - Escherichia coli - Bacteriology
- 质疑
- Engineering Saccharomyces cerevisiae for Hyperproduction of β-Amyrin by Mitigating the Inhibition Effect of Squalene on β-Amyrin Synthase被引量：35收藏
- 作者：Du, Meng-Meng Zhu, Zhan-Tao Zhang, Ge-Ge Zhao, Yun-Qiu Gao, Bei Tao, Xin-Yi Liu, Min Ren, Yu-Hong Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2022
- 关键词：beta-amyrin squalene inhibition peroxisome compartmentalization competition pathway Saccharomyces cerevisiae
- 质疑
- Improving the Expression of Recombinant Proteins in E. coli BL21 (DE3) under Acetate Stress: An Alkaline pH Shift Approach被引量：35收藏
- 作者：Wang, Hengwei Wang, Fengqing Wang, Wei Yao, Xueling Wei, Dongzhi Cheng, Hairong Deng, Zixin
- 机构：Zhejiang Ocean Univ;Shanghai Jiao Tong Univ;E China Univ Sci & Technol
- 来源：PLOS ONE 2014
- 质疑
- Biotransformation of diosgenin to nuatigenin-type steroid by a newly isolated strain, Streptomyces virginiae IBL-14被引量：31收藏
- 作者：Wang, Feng-Qing Li, Bo Wang, Wei Zhang, Cheng-Gang Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2007
- 关键词：diosgenin diosgenone isonuatigenone nuatigenin-type steroid tertiary hydroxylation Streptomyces virginiae
- 质疑
- Engineered 3-ketosteroid 9a-hydroxylases in Mycobacterium neoaurum: An efficient platform for production of steroid drugs被引量：30收藏
- 作者：Liu, Hao-Hao Xu, Li-Qin Yao, Kang Xiong, Liang-Bin Tao, Xin-Yi Liu, Min Wang, Feng-Qing Wei, Dong-Zhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied and Environmental Microbiology 2018
- 关键词：Metabolic engineering - Lipids - Alcohols - Metabolism
- 质疑
- Role Identification and Application of SigD in the Transformation of Soybean Phytosterol to 9α-Hydroxy-4-androstene-3,17-dione in Mycobacterium neoaurum被引量：30收藏
- 作者：Xiong, Liang-Bin Liu, Hao-Hao Xu, Li-Qin Wei, Dong-Zhi Wang, Feng-Qing
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2017
- 关键词：mycobacteria 9a-hydroxy-4-androstene-3,17-dione (9-OHAD) soybean phytosterol transcription factor sigD choM2
- 质疑
- Characterization of P450 FcpC, the enzyme responsible for bioconversion of diosgenone to isonuatigenone in Streptomyces virginiae IBL-14被引量：29收藏
- 作者：Wang, Wei Wang, Feng-Qing Wei, Dong-Zhi
- 机构： State Key Lab. of Bioreactor Engineering
- 来源：Applied and Environmental Microbiology 2009
- 关键词：Escherichia coli
- 质疑
- Accumulation of androstadiene-dione by overexpression of heterologous 3-ketosteroid Δ1-dehydrogenase in Mycobacterium neoaurum NwIB-01被引量：29收藏
- 作者：Wei, Wei Fan, Shu-Yue Wang, Feng-Qing Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 2014
- 关键词：1,4-Androstadiene-3,17-dione 3-Ketosteroid Delta(1)-dehydrogenase 4-Androstene-3,17-dione Gene overexpression Phytosterols Plasmid stability
- 质疑
