成果/Result
- A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules被引量：378收藏
- 作者：Liang, Mindong Li, Zilong Wang, Weishan Liu, Jiakun Liu, Leshi Zhu, Guoliang Karthik, Loganathan Wang, Man Wang, Ke-Feng Wang, Zhong Yu, Jing Shuai, Yuting Yu, Jiaming Zhang, Lu Yang, Zhiheng Li, Chuan Zhang, Qian Shi, Tong Zhou, Liming Xie, Feng Dai, Huanqin Liu, Xueting Zhang, Jingyu Liu, Guang Zhuo, Ying Zhang, Buchang Liu, Chenli Li, Shanshan Xia, Xuekui Tong, Yaojun Liu, Yanwen Alterovitz, Gil Tan, Gao-Yi Zhang, Li-Xin
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Anhui Univ;Chinese Acad Sci;Chinese Acad Sci
- 来源：NATURE COMMUNICATIONS 2019
- Harnessing the intracellular triacylglycerols for titer improvement of polyketides in Streptomyces被引量：143收藏
- 作者：Wang, Weishan Li, Shanshan Li, Zilong Zhang, Jingyu Fan, Keqiang Tan, Gaoyi Ai, Guomin Lam, Sin Man Shui, Guanghou Yang, Zhiheng Lu, Hongzhong Jin, Pinjiao Li, Yihong Chen, Xiangyin Xia, Xuekui Liu, Xueting Dannelly, H. Kathleen Yang, Chen Yang, Yi Zhang, Siliang Alterovitz, Gil Xiang, Wensheng Zhang, Lixin
- 机构：Chinese Acad Sci;East China Univ Sci & Technol;Chinese Acad Agr Sci;Chinese Acad Sci;Qilu Univ Technol
- 来源：NATURE BIOTECHNOLOGY 2020
- 关键词：Ketones - Metabolism - Biochemistry - Bacteria - Metabolites - Glycerol
- A versatile biosensing platform coupling CRISPR-Cas12a and aptamers for detection of diverse analytes被引量：88收藏
- 作者：Zhao, Xiangxiang Li, Shanshan Liu, Guang Wang, Zhong Yang, Zhiheng Zhang, Quanwei Liang, Mindong Liu, Jiakun Li, Zilong Tong, Yaojun Zhu, Guoliang Wang, Xinye Jiang, Lan Wang, Weishan Tan, Gao-Yi Zhang, Lixin
- 机构：East China Univ Sci & Technol ECUST;East China Univ Sci & Technol ECUST;Chinese Acad Agr Sci;Chinese Acad Sci;Shanghai Jiao Tong Univ
- 来源：SCIENCE BULLETIN 2021
- 关键词：CRISPR-Cas12a Aptamer Biosensing platform Diverse analyte Alpha fetoprotein detection Cocaine detection
- Heterologous Biosynthesis of Spinosad: An Omics-Guided Large, Polyketide Synthase Gene Cluster Reconstitution in Streptomyces被引量：82收藏
- 作者：Tan, Gao-Yi Deng, Kunhua Liu, Xinhua Tao, Hui Chang, Yingying Chen, Jia Chen, Kai Sheng, Zhi Deng, Zixin Liu, Tiangang
- 机构：Wuhan Univ;Wuhan Univ;Wuhan Inst Biotechnol;East China Univ Sci & Technol;Shenyang Res Inst Chem Ind Ltd Co
- 来源：ACS SYNTHETIC BIOLOGY 2017
- 关键词：spinosyn Streptornyces heterologous production metabolomics proteomics module optimization
- Rational synthetic pathway refactoring of natural products biosynthesis in actinobacteria被引量：55收藏
- 作者：Tan, Gao-Yi Liu, Tiangang
- 机构：Wuhan Univ;Wuhan Univ;Wuhan Inst Biotechnol;East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2017
- 关键词：Actinobacteria Natural product Synthetic pathway Refactoring Metabolic engineering
- Activating cryptic biosynthetic gene cluster through a CRISPR-Cas12a-mediated direct cloning approach被引量：54收藏
- 作者：Liang, Mindong Liu, Leshi Xu, Fei Zeng, Xiaoqian Wang, Runjun Yang, Jinling Wang, Weishan Karthik, Loganathan Liu, Jiakun Yang, Zhiheng Zhu, Guoliang Wang, Shuliu Bai, Linquan Tong, Yaojun Liu, Xueting Wu, Min Zhang, Li-Xin Tan, Gao-Yi
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Zhejiang Univ;Zhejiang Univ;Huzhou Univ
- 来源：NUCLEIC ACIDS RESEARCH 2022
- Polyketide pesticides from actinomycetes被引量：52收藏
- 作者：Li, Shanshan Yang, Bowen Tan, Gao-Yi Ouyang, Li-Ming Qiu, Shiwen Wang, Weishan Xiang, Wensheng Zhang, Lixin
- 机构：Chinese Acad Agr Sci;East China Univ Sci & Technol;Chinese Acad Sci;Northeast Agr Univ;Univ Chinese Acad Sci
- 来源：CURRENT OPINION IN BIOTECHNOLOGY 2021
- 关键词：Crop protection - Metabolites - Bacteria
- Harnessing a previously unidentified capability of bacterial allosteric transcription factors for sensing diverse small molecules in vitro被引量：42收藏
- 作者：Cao, Jiaqian Yao, Yongpeng Fan, Keqiang Tan, Gaoyi Xiang, Wensheng Xia, Xuekui Li, Shanshan Wang, Weishan Zhang, Lixin
- 机构：State Key Lab Microbial Resources;Chinese Acad Sci;East China Univ Sci & Technol;Chinese Acad Agr Sci;Qilu Univ Technol
- 来源：SCIENCE ADVANCES 2018
- 关键词：Biosensors - Transcription - DNA - Binding sites - Signal transduction
- High-yield porphyrin production through metabolic engineering and biocatalysis被引量：34收藏
- 作者：Chen, Haihong Wang, Yaohong Wang, Weishan Cao, Ting Zhang, Lu Wang, Zhengduo Chi, Xuran Shi, Tong Wang, Huangwei He, Xinwei Liang, Mindong Yang, Mengxue Jiang, Wenyi Lv, Dongyuan Yu, Jiaming Zhu, Guoliang Xie, Yongtao Gao, Bei Wang, Xinye Liu, Xueting Li, Youyuan Ouyang, Limin Zhang, Jingyu Liu, Huimin Li, Zilong Tong, Yaojun Xia, Xuekui Tan, Gao-Yi Zhang, Lixin
- 机构：East China Univ Sci & Technol;Chinese Acad Sci;Shanghai Jiao Tong Univ;Qilu Univ Technol
- 来源：NATURE BIOTECHNOLOGY 2025
- 关键词：Catalysis - Diagnosis - Fermentation - Medical applications - Metabolic engineering
- Learn from microbial intelligence for avermectins overproduction被引量：32收藏
- 作者：Gao, Qiang Tan, Gao-Yi Xia, Xuekui Zhang, Lixin
- 机构：East China Univ Sci & Technol;Tianjin Univ Sci & Technol;Shandong Acad Sci;Qingdao Natl Lab Marine Sci & Technol
- 来源：CURRENT OPINION IN BIOTECHNOLOGY 2017
- In vitro allosteric transcription factor-based biosensing被引量：31收藏
- 作者：Li, Shanshan Li, Zilong Tan, Gao-Yi Xin, Zhenguo Wang, Weishan
- 机构：Chinese Acad Agr Sci;Chinese Acad Sci;East China Univ Sci & Technol;East China Univ Sci & Technol;Univ Chinese Acad Sci
- 来源：TRENDS IN BIOTECHNOLOGY 2023
- 关键词：Analytic equipment - Molecules - Transcription - Transcription factors
- Systematic development of a highly efficient cell factory for 5-aminolevulinic acid production被引量：28收藏
- 作者：Zhou, Houming Zhang, Chengyu Li, Zilong Xia, Menglei Li, Zhenghong Wang, Zhengduo Tan, Gao-Yi Luo, Ying Zhang, Lixin Wang, Weishan
- 机构：East China Univ Sci & Technol;Chinese Acad Sci;Tianjin Univ Sci & Technol
- 来源：TRENDS IN BIOTECHNOLOGY 2024
- 关键词：Agriculture - Metabolic engineering - Metabolism
- Phosphate limitation increases coenzyme Q10 production in industrial Rhodobacter sphaeroides HY01被引量：25收藏
- 作者：Zhang, Lu Liu, Leshi Wang, Ke-Feng Xu, Lan Zhou, Liming Wang, Weishan Li, Chuan Xu, Zheng Shi, Tong Chen, Haihong Li, Yuanhang Xu, Hui Yang, XiuLiang Zhu, Zhichun Chen, Biqin Li, Dan Zhan, Guanghuang Zhang, Si-Liang Zhang, Li-Xin Tan, Gao-Yi
- 机构：ECUST;ECUST;Chinese Acad Sci;Chinese Acad Sci;Chinese Acad Sci
- 来源：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY 2019
- 关键词：R sphaeroides CoQ(10) Phosphate limitation Overproduction Scale-up Transcriptome
- Engineering thermophilic Geobacillus thermoglucosidasius for riboflavin production被引量：24收藏
- 作者：Yang, Zhiheng Sun, Qingqing Tan, Gaoyi Zhang, Quanwei Wang, Zhengduo Li, Chuan Qi, Fengxian Wang, Weishan Zhang, Lixin Li, Zilong
- 机构：East China Univ Sci & Technol;Chinese Acad Sci
- 来源：MICROBIAL BIOTECHNOLOGY 2021
- Integrating PCR-free amplification and synergistic sensing for ultrasensitive and rapid CRISPR/Cas12a-based SARS-CoV-2 antigen detection被引量：24收藏
- 作者：Zhao, Xiangxiang Wang, Zhengduo Yang, Bowen Li, Zilong Tong, Yaojun Bi, Yuhai Li, Zhenghong Xia, Xuekui Chen, Xiangyin Zhang, Lixin Wang, Weishan Tan, Gao-Yi
- 机构：East China Univ Sci & Technol ECUST;East China Univ Sci & Technol ECUST;Chinese Acad Sci;Shanghai Jiao Tong Univ;Shanghai Jiao Tong Univ
- 来源：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY 2021
- 关键词：CRISPR Cas12a Aptamer Antigen detection SARS-CoV-2 PCR-Free amplification Synergistic sensing
- A thermostable type I-B CRISPR-Cas system for orthogonal and multiplexed genetic engineering被引量：22收藏
- 作者：Yang, Zhiheng Li, Zilong Li, Bixiao Bu, Ruihong Tan, Gao-Yi Wang, Zhengduo Yan, Hao Xin, Zhenguo Zhang, Guojian Li, Ming Xiang, Hua Zhang, Lixin Wang, Weishan
- 机构：China Univ Sci & Technol ECUST;East China Univ Sci & Technol ECUST;Chinese Acad Sci;Ocean Univ China;Univ Chinese Acad Sci
- 来源：NATURE COMMUNICATIONS 2023
- Screening and engineering of high-activity promoter elements through transcriptomics and red fluorescent protein visualization in Rhodobacter sphaeroides被引量：19收藏
- 作者：Shi, Tong Zhang, Lu Liang, Mindong Wang, Weishan Wang, Kefeng Jiang, Yue Liu, Jing He, Xinwei Yang, Zhiheng Chen, Haihong Li, Chuan Lv, Dongyuan Zhou, Liming Chen, Biqin Li, Dan Zhang, Li-Xin Tan, Gao-Yi
- 机构：East China Univ Sci & Technol ECUST;East China Univ Sci & Technol ECUST;Chinese Acad Sci;Chinese Acad Sci;Inner Mongolia Kingdomway Pharmaceut Co Ltd
- 来源：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY 2021
- 关键词：Rhodobacter sphaeroides Promoter library Transcriptomics Co-enzyme Q 10 Red fluorescent protein
- Assessment of light distribution model for marine red microalga Porphyridium purpureum for sustainable production in photobioreactor被引量：17收藏
- 作者：Li, Shaohua Huang, Jianke Ji, Liang Chen, Cheng Wu, Ping Zhang, Wei Tan, Gaoyi Wu, Haizhen Fan, Jianhua
- 机构：East China Univ Sci & Technol;Hohai Univ;Hunan Inst Engn;East China Univ Sci & Technol
- 来源：ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 2021
- 关键词：Red algae Biomass productivity Sustainability production Light distribution model Volume-averaged light intensity
- Genome-guided investigation of anti-inflammatory sesterterpenoids with 5-15 trans-fused ring system from phytopathogenic fungi被引量：15收藏
- 作者：Jiang, Lan Zhu, Guoliang Han, Jianying Hou, Chengjian Zhang, Xue Wang, Zhixin Yuan, Weize Lv, Kangjie Cong, Zhanren Wang, Xinye Chen, Xiangyin Karthik, Loganathan Yang, Huanting Wang, Xuyuan Tan, Gaoyi Liu, Guang Zhao, Liya Xia, Xuekui Liu, Xiangyang Gao, Shushan Ma, Lei Liu, Mei Ren, Biao Dai, Huanqin Quinn, Ronald J. Hsiang, Tom Zhang, Jingyu Zhang, Lixin Liu, Xueting
- 机构：East China Univ Sci & Technol;Chinese Acad Sci;Griffith Univ;Qilu Univ Technol;T3 Biosci LLC
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2021
- 关键词：Fungi Bifunctional terpene synthases Terpenoids Anti-inflammatory
- Unleashing the potential: type I CRISPR-Cas systems in actinomycetes for genome editing被引量：13收藏
- 作者：Wang, Shuliu Zeng, Xiaoqian Jiang, Yue Wang, Weishan Bai, Linquan Lu, Yinhua Zhang, Lixin Tan, Gao-Yi
- 机构：East China Univ Sci & Technol ECUST;Chinese Acad Sci;Chinese Acad Sci;Shanghai Jiao Tong Univ;Shanghai Normal Univ
- 来源：NATURAL PRODUCT REPORTS 2024
- 响应面法设计优化阿维菌素化学合成发酵培养基被引量：10收藏
- 作者：胡栋 柯灵超 张敬宇 谭高翼 张立新 高强
- 机构：工业发酵微生物教育部重点实验室;生物反应器工程国家重点实验室
- 来源：《中国抗生素杂志》 2018
- 关键词：阿维链霉菌 阿维菌素 化学合成培养基 响应面法 设计优化
- 摘要：目的设计优化一种化学合成发酵培养基,为阿维菌素产生菌生理生化研究提供基础。方法在单因素实验的基础上运用响应面分析的方法,对9种因素进行Plackett-Burman设计筛选得到3个显著因子,并对其进行最陡爬坡试验和Box...
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- 微生物,高智商,大产业——合成生物学助力阿维菌素的高效智能制造被引量：9收藏
- 作者：刘乐诗 谭高翼 王为善 李珊珊 夏雪奎 刘昌衡 代焕琴 王泽建 张嗣良 张立新
- 机构：华东理工大学生物反应器工程国家重点实验室;中国科学院微生物研究所;中国农业科学院植物保护研究所;齐鲁工业大学(山东省科学院)生物研究所
- 来源：《生命科学》 2019
- 关键词：微生物药物 阿维菌素 合成生物学 高效制造 智能化
- 摘要：合成生物学研究具有两种属性,即生命本质的认识提升属性与生物制造属性。以阿维菌素产生的生命过程复杂性为例,几乎可用"高智商"来形容。通过系统认知微生物的"智商",从"格物致知"到"建物致用",实现师法自然,让微生物更高效地...
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- A rapid nucleic acid detection platform based on phosphorothioate-DNA and sulfur binding domain被引量：9收藏
- 作者：Shuai, Yuting Ju, Yi Li, Yuanhang Ma, Dini Jiang, Lan Zhang, Jingyu Tan, Gao-Yi Liu, Xueting Wang, Shenlin Zhang, Lixin Liu, Guang
- 机构：East China Univ Sci & Technol ECUST;East China Univ Sci & Technol ECUST;Shanghai Jiao Tong Univ
- 来源：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY 2023
- 关键词：Phosphorothioate Sulfur binding domain Nucleic acid detection
- 构建“合成生物学”课程群培养面向生物制造的复合型创新人才被引量：8收藏
- 作者：谭高翼 张立新 张敬宇 王启要 吴辉 庄英萍 欧阳立明 朱国良 王馨叶 刘雪婷
- 机构：华东理工大学生物工程学院
- 来源：《生物工程学报》 2024
- 关键词：合成生物学 课程群 复合型创新人才 国际基因工程机器大赛
- 摘要：合成生物学(Synthetic Biology)作为一门新兴学科,广受关注且发展迅速,深刻影响着整个生命科学及工程技术领域的发展。与此同时,随着新工科建设的发展,加快培养复合型创新人才是对我国高等教育提出的新任务和新使命...
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- 国际基因工程机器大赛对本科生综合能力培养模式的探索被引量：8收藏
- 作者：王启要 李鹏飞 高淑红 李友元 吴辉 谭高翼 范建华 周勉 张立新 庄英萍
- 机构：华东理工大学生物工程学院
- 来源：《生物工程学报》 2021
- 关键词：国际基因工程机器比赛 生物创客 创新能力培养体系 合成生物学
- 摘要：华东理工大学生物工程学院从高水平专业比赛出发,以学生的兴趣为切入点,依托强大的科研和教学基地,构建具有"生物创客"精神的人才培养体系和创新实践能力培养体系。充分发挥学生主观能动性,提升了生物工程类相关专业本科生的科研素养...
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- Expanding horizons of CRISPR applications beyond genome editing被引量：6收藏
- 作者：Liang, Yu Tong, Shengkun Zhang, Jingyu Tan, Gao-Yi Zhang, Lixin Lee, Sang Yup Tong, Yaojun
- 机构：Shanghai Jiao Tong Univ;East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol SCI;Korea Adv Inst Sci & Technol KAIST;Korea Adv Inst Sci & Technol
- 来源：TRENDS IN GENETICS 2025
- Scalable secondary metabolite production in Streptomyces using a plug-and-play system被引量：5收藏
- 作者：Yang, Bowen Li, Zilong Zhang, Jingyu Qiu, Shiwen Liu, Xueting Liang, Zonglin Yan, Hao Zhang, Yanyan Liu, Lihong Xia, Bing Bao, Lianqun Li, Defeng Zhou, Shanshan Corre, Christophe Zhang, Chengyu Lu, Yinhua Tan, Gao-Yi Xia, Xuekui Li, Shanshan Zhang, Lixin Wang, Weishan
- 机构：East China Univ Sci & Technol ECUST;East China Univ Sci & Technol ECUST;Chinese Acad Sci;Beijing Key Lab Genet Element Biosourcing & Intell;Chinese Acad Agr Sci
- 来源：NATURE BIOTECHNOLOGY 2025
- 关键词：Biomolecules - Strain
- Engineering the Methylerythritol Phosphate Pathway and Using a Temporal Promoter for Enhanced Lycopene Production in Rhodobacter sphaeroides HY01被引量：3收藏
- 作者：He, Xinwei Wang, Dan Liu, Jing Shi, Tong Wang, Weishan Chen, Biqin Li, Dan Zhang, Lixin Tan, Gao-Yi
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Chinese Acad Sci;Inner Mongolia Kingdomway Pharmaceut Co Ltd;Shanghai Collaborat Innovat Ctr Biomfg Technol SCI
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2024
- 关键词：Rhodobactersphaeroides coenzyme Q(10) terpenoids lycopene MEP pathway temporal promoter
- An in vitro CRISPR-Cas12a-mediated protocol for direct cloning of large DNA fragments被引量：3收藏
- 作者：Zeng, Xiaoqian Wang, Shuliu Liang, Mindong Wang, Weishan Jiang, Yue Xu, Fei Liu, Leshi Yan, Hao Tong, Yaojun Zhang, Lixin Tan, Gao-Yi
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Chinese Acad Sci;Zhejiang Univ;Zhejiang Univ
- 来源：STAR PROTOCOLS 2023
- Rational spatial rewiring of key enzymes enhances α-santalene production in Saccharomyces cerevisiae被引量：2收藏
- 作者：Tan, Wenwen Tong, Shengkun Gao, Yaojie Wang, Jing Zhang, Jingyu Xie, Zhiping Dai, Huanqin Liang, Yu Tan, Gao-Yi Zhang, Lixin Tong, Yaojun
- 机构：East China Univ Sci & Technol;Shanghai Jiao Tong Univ;Shanghai Collaborat Innovat Ctr Biomfg Technol SCI;Chinese Acad Sci;Univ Chinese Acad Sci
- 来源：BIORESOURCE TECHNOLOGY 2025
- 关键词：Subcellular enzyme localization Spatial engineering alpha-Santalene Saccharomyces cerevisiae Metabolic engineering Synthetic biology
