成果/Result
- High cell density fermentation of Gluconobacter oxydans DSM 2003 for glycolic acid production被引量：48收藏
- 作者：Wei, Guodong Yang, Xuepeng Gan, Tula Zhou, Wenyu Lin, Jinping Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Zhengzhou Univ Light Ind
- 来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2009
- 关键词：Gluconobacter oxydans Uniform design Fed-batch High-density fermentation Bioconversion Ethylene glycol Glycolic acid
- Purification and Characterization of an Organic Solvent-Tolerant Lipase from Pseudomonas aeruginosa CS-2被引量：43收藏
- 作者：Peng, Ren Lin, Jinping Wei, Dongzhi
- 机构：E China Univ Chem Technol;Jiangxi Normal Univ
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2010
- 关键词：Organic solvent-tolerant lipase Pseudomonas aeruginosa CS-2
- Pyrroloquinoline quinone biosynthesis in Escherichia coli through expression of the Gluconobacter oxydans pqqABCDE gene cluster被引量：38收藏
- 作者：Yang, Xue-Peng Zhong, Gui-Fang Lin, Jin-Ping Mao, Duo-Bin Wei, Dong-Zhi
- 机构：Zhengzhou Univ Light Ind;E China Univ Sci & Technol
- 来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2010
- 关键词：Biosynthesis Expression Gene cluster Pyrroloquinoline quinone
- Enhanced production of dihydroxyacetone from glycerol by overexpression of glycerol dehydrogenase in an alcohol dehydrogenase-deficient mutant of Gluconobacter oxydans被引量：37收藏
- 作者：Li, Ming-hua Wu, Jian Liu, Xu Lin, Jin-ping Wei, Dong-zhi Chen, Hao
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresource Technology 2010
- 关键词：Bioconversion - Polymerase chain reaction - Productivity - Enzyme activity - Genes
- Improving the catalytic activity of lipase LipK107 from Proteus sp. by site-directed mutagenesis in the lid domain based on computer simulation被引量：34收藏
- 作者：Gao, Bei Xu, Tao Lin, Jinping Zhang, Lujia Su, Erzheng Jiang, Zhengbing Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering; College of Life Science
- 来源：Journal of Molecular Catalysis B: Enzymatic 2011
- 关键词：Lipases - Palmitic acid - Mutagenesis - Catalysis
- Development of recombinant Escherichia coli whole-cell biocatalyst expressing a novel alkaline lipase-coding gene from Proteus sp. for biodiesel production被引量：33收藏
- 作者：Gao, Bei Su, Erzheng Lin, Jinping Jiang, Zhengbing Ma, Yushu Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Biotechnology 2009
- 关键词：Catalysts - Cloning - Cytology - Gene encoding - Temperature - Biodiesel - Purification - Alkalinity
- Optimization of immobilization for selective oxidation of benzyl alcohol by Gluconobacter oxydans using response surface methodology被引量：32收藏
- 作者：Wu, Jian Wang, Jia-Le Li, Min-Hua Lin, Jin-Ping Wei, Dong-Zhi
- 机构： New World Institute of Biotechnology
- 来源：Bioresource Technology 2010
- 关键词：Alginate - Cell immobilization - Surface properties
- Functions of membrane-bound alcohol dehydrogenase and aldehyde dehydrogenase in the bio-oxidation of alcohols in Gluconobacter oxydans DSM 2003被引量：31收藏
- 作者：Wei, Liu-Jing Zhou, Ji-Lai Zhu, Dan-Ni Cai, Bai-Yi Lin, Jin-Ping Hua, Qiang Wei, Dong-Zhi
- 机构： State Key Laboratory of Bioreactor Engineering; State Key Laboratory of Bioreactor Engineering
- 来源：Biotechnology and Bioprocess Engineering 2012
- 关键词：Enzymes - Oxidation - Aldehydes
- Towards the computational design and engineering of enzyme enantioselectivity: A case study by a carbonyl reductase from Gluconobacter oxydans被引量：31收藏
- 作者：Deng, Jian Yao, Zhiqiang Chen, Kangling Yuan, Y. Adam Lin, Jinping Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Natl Univ Singapore
- 来源：JOURNAL OF BIOTECHNOLOGY 2016
- 关键词：Carbonyl reductase Enantiorecognition Computational design OPBE Improved enantioselectivity
- Characterization of a Putative Stereoselective Oxidoreductase from Gluconobacter oxydans and Its Application in Producing Ethyl (R)-4-Chloro-3-Hydroxybutanoate Ester被引量：26收藏
- 作者：Liu, Xu Chen, Rong Yang, Zhongwei Wang, Jiale Lin, Jinping Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Hangzhou Normal Univ
- 来源：MOLECULAR BIOTECHNOLOGY 2014
- 关键词：Gluconobacter oxydans Oxidoreductase Ethyl (R)-4-chloro-3-hydroxybutanoate ester
- A novel strain Pseudomonas aeruginosa CS-2 producing an organic solvent-tolerant lipase被引量：25收藏
- 作者：Peng, Ren Lin, Jinping Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering; College of Life Science
- 来源：Advanced Materials Research 2012
- 关键词：Bacteria - Lipases - Hexane - Magnesium compounds - Organic solvents - Chlorine compounds
- Identification of a novel promoter gHp0169 for gene expression in Gluconobacter oxydans被引量：25收藏
- 作者：Shi, Lulu Li, Kefei Zhang, Huan Liu, Xu Lin, Jinping Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2014
- 关键词：Gluconobacter oxydans Promoter gHp0169 Gene overexpression
- Application of comparative proteome analysis to reveal influence of cultivation conditions on asymmetric bioreduction of β-keto ester by Saccharomyces cerevisiae被引量：24收藏
- 作者：Lin, Jinping Liu, Qinghai Su, Erzheng Wei, Dongzhi Yang, Shengli
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied Microbiology and Biotechnology 2008
- 关键词：Batch cell culture - Cultivation - Esters - Metabolism - Cells - Enzymes
- Enhancement of cell growth and glycolic acid production by overexpression of membrane-bound alcohol dehydrogenase in Gluconobacter oxydans DSM 2003被引量：23收藏
- 作者：Zhang, Huan Shi, Lulu Mao, Xinlei Lin, Jinping Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2016
- 关键词：Gluconobacter oxydans Alcohol dehydrogenase Respiratory chain Glycolic acid
- Identification of a yeast old yellow enzyme for highly enantioselective reduction of citral isomers to (R)-citronellal被引量：22收藏
- 作者：Meng, Liandan Lin, Jinping Mang, Baoqi Kuang, Yuyao Wei, Dongzhi
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2018
- 关键词：Enoate reductase Old yellow enzyme (OYE) Citral (R)-Citronellal Enantioselectivity Asymmetric reduction
- Membrane-bound pyrroloquinoline quinone-dependent dehydrogenase in Gluconobacter oxydans M5, responsible for production of 6-(2-hydroxyethyl) amino-6-deoxy-L-sorbose被引量：21收藏
- 作者：Yang, Xue-Peng Wei, Liu-Jing Lin, Jin-Ping Yin, Bo Wei, Dong-Zhi
- 机构： State Key Laboratory of Bioreactor Engineering; College of Food and Biological Engineering; East China University of Science and Biotechnology
- 来源：Applied and Environmental Microbiology 2008
- 关键词：Alcohols - Microbiology
- Construction of a Novel Shuttle Vector for Use in Gluconobacter oxydans被引量：21收藏
- 作者：Zhang, Lin Lin, Jinping Ma, Yushu Wei, Dongzhi Sun, Ming
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol
- 来源：MOLECULAR BIOTECHNOLOGY 2010
- 关键词：Shuttle vector Gluconobacter oxydans Cryptic plasmid Expression
- Adsorptive bioconversion of ethylene glycol to glycolic acid by Gluconobacter oxydans DSM 2003被引量：19收藏
- 作者：Wei, Guodong Yang, Xuepeng Zhou, Wenyu Lin, Jinping Wei, Dongzhi
- 机构： State Key Lab of Bioreactor Engineering; College of Food and Biological Engineering
- 来源：Biochemical Engineering Journal 2009
- 关键词：Polyols - Bioconversion - Ethylene - Ion exchange resins
- Lipase-catalyzed continuous ring-opening polymerization of e-caprolactone in a packed-bed reactor被引量：18收藏
- 作者：Zhang, M.J. Su, E.Z. Lin, J.P. Wei, D.Z.
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Chemical and Biochemical Engineering Quarterly 2012
- 关键词：Packed beds - Chemical reactors - Polydispersity - Monomers - Catalysis
- Identification of the enzymes responsible for 3-hydroxypropionic acid formation and thEIr use in improving 3-hydroxypropionic acid production in Gluconobacter oxydans DSM 2003被引量：18收藏
- 作者：Zhu, Jiawei Xie, Jingli Wei, Liujing Lin, Jinping Zhao, Li Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCE TECHNOLOGY 2018
- 关键词：3-Hydroxypropionic acid Gluconobacter oxydans Membrane-bound alcohol dehydrogenase Membrane-bound aldehyde dehydrogenase Metabolic pathway
- Cloning, expression, and characterization of an anti-Prelog stereospecific carbonyl reductase from Gluconobacter oxydans DSM2343被引量：16收藏
- 作者：Chen, Rong Liu, Xu Wang, Jiale Lin, Jinping Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Hangzhou Normal Univ
- 来源：ENZYME AND MICROBIAL TECHNOLOGY 2015
- 关键词：Carbonyl reductase Asymmetric reduction Anti-Prelog rule Molecular modeling
- Oxidation of ethylene glycol to glycolaldehyde using a highly selective alcohol dehydrogenase from Gluconobacter oxydans被引量：16收藏
- 作者：Zhang, Xingxing Zhang, Baoqi Lin, Jinping Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2015
- 关键词：Alcohol dehydrogenase NADH oxidase-2 Ethylene glycol Glycolaldehyde Cofactor regeneration
- Characterization of an ene-reductase from Meyerozyma guilliermondii for asymmetric bioreduction of α,β-unsaturated compounds被引量：16收藏
- 作者：Zhang, Baoqi Zheng, Liandan Lin, Jinping Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2016
- 关键词：Bioreduction (R)-Carvone Dihydrocarvone Ene-reductase NADPH dehydrogenase Old yellow enzyme alpha,beta-Unsaturated compounds
- Engineered Expression Vectors Significantly Enhanced the Production of 2-Keto-D-gluconic Acid by Gluconobacter oxidans被引量：16收藏
- 作者：Shi, Yuan-yuan Li, Ke-fei Lin, Jin-ping Yang, Sheng-li Wei, Dong-zhi
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2015
- 关键词：expression vectors plasmid copy number Gluconobacter oxidans overexpression 2-keto-D-gluconic acid
- Characterization of Enzymes in the Oxidation of 1,2-Propanediol to d-(-)-Lactic Acid by Gluconobacter oxydans DSM 2003被引量：15收藏
- 作者：Wei, Liujing Yang, Xuepeng Gao, Keliang Lin, Jinping Yang, Shengli Hua, Qiang Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Zhengzhou Univ Light Ind
- 来源：MOLECULAR BIOTECHNOLOGY 2010
- 关键词：Gluconobacter oxydans Alcohol dehydrogenase Aldehyde dehydrogenase 1,2-Propanediol D-(-)-lactic acid
- Efficient bioreductive production of (R)-N-Boc-3-hydroxypiperidine by a carbonyl reductase被引量：15收藏
- 作者：Chen, Li-Feng Zhang, Yi-Ping Fan, Hai-Yang Wu, Kai Lin, Jin-Ping Wang, Hua-Lei Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci
- 来源：CATALYSIS COMMUNICATIONS 2017
- 关键词：(R)-N-Boc-3-hydroxypiperidine Carbonyl reductase Asymmetric reduction Cofactor-recycling system
- Enhancement of ethyl (S)-4-chloro-3-hydroxybutanoate production at high substrate concentration by in situ resin adsorption被引量：14收藏
- 作者：Chen, Li-Feng Fan, Hai-Yang Zhang, Yi-Ping Wei, Wei Lin, Jin-Ping Wei, Dong-Zhi Wang, Hua-Lei
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2017
- 关键词：Ethyl (S)-4-chloro-3-hydroxybutanoate Carbonyl reductase Asymmetric reduction Product inhibition Resin adsorption
- Characterization of a novel dextran produced by Gluconobacter oxydans DSM 2003被引量：14收藏
- 作者：Wang, Shu Mao, Xiangzhao Wang, Hualei Lin, Jinping Li, Fuli Wei, Dongzhi
- 机构：Chinese Acad Sci;Ocean Univ China;E China Univ Sci & Technol;Chinese Acad Sci
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2011
- 关键词：Dextran Gluconobacter oxydans Monosaccharide composition Structure analysis
- A genomic search approach to identify carbonyl reductases in Gluconobacter oxydans for enantioselective reduction of ketones被引量：14收藏
- 作者：Chen, Rong Liu, Xu Lin, Jinping Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Hangzhou Normal Univ
- 来源：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 2014
- 关键词：carbonyl reductase enantioselective reduction chiral alcohols
- Computational design of short-chain dehydrogenase Gox2181 for altered coenzyme specificity被引量：14收藏
- 作者：Cui, Dongbing Zhang, Lujiang Yao, Zhiqiang Liu, Xu Lin, Jinping Yuan, Adam Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Natl Univ Singapore
- 来源：JOURNAL OF BIOTECHNOLOGY 2013
- 关键词：Computational design Altered coenzyme specificity Molecular dynamics simulation Site-directed mutagenesis
