成果/Result
- Central pathway engineering for enhanced succinate biosynthesis from acetate in Escherichia coli被引量：62收藏
- 作者：Huang, Bing Yang, Hao Fang, Guochen Zhang, Xing Wu, Hui Li, Zhimin Ye, Qin
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol;China Natl Light Ind Council
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2018
- 关键词：acetate cofactor engineering Escherichia coli metabolic engineering succinate
- Bioethanol production from starchy biomass by direct fermentation using Saccharomyces diastaticus in batch free and immobilized cell systems被引量：57收藏
- 作者：Kilonzo, Peter M. Margaritis, Argyrios Yu, Juntang Ye, Qin
- 机构： Department of Chemical and Biochemical Engineering; Biochemical Engineering Research Institute
- 来源：International Journal of Green Energy 2007
- 关键词：Biomass - Bioreactors - Cell immobilization - Conversion efficiency - Fermentation - Starch - Yeast
- Development of succinic acid production from corncob hydrolysate by Actinobacillus succinogenes被引量：54收藏
- 作者：Yu, Jie Li, Zhimin Ye, Qin Yang, Yong Chen, Shulin
- 机构：E China Univ Sci & Technol;Chinese Acad Sci
- 来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2010
- 关键词：Actinobacillus succinogenes Succinic acid Corncob hydrolysate Xylose Optimization
- Enhanced succinate production from glycerol by engineered Escherichia coli strains被引量：47收藏
- 作者：Li, Qing Wu, Hui Li, Zhimin Ye, Qin
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol
- 来源：BIORESOURCE TECHNOLOGY 2016
- 关键词：Succinate Escherichia coli Glycerol Phosphoenolpyruvate carboxykinase Two-stage fermentation
- Simultaneous saccharification and fermentation of cassava to succinic acid by Escherichia coli NZN111被引量：46收藏
- 作者：Chen, Cuixia Ding, Shaopeng Wang, Dezheng Li, Zhimin Ye, Qin
- 机构：E China Univ Sci & Technol
- 来源：BIORESOURCE TECHNOLOGY 2014
- 关键词：Succinic acid Simultaneous saccharification and fermentation Cassava Escherichia coli NZN111
- Enhanced production of 3-hydroxypropionic acid from glucose via malonyl-CoA pathway by engineered Escherichia coli被引量：45收藏
- 作者：Cheng, Zhuan Jiang, Jiaqi Wu, Hui Li, Zhimin Ye, Qin
- 机构：E China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol
- 来源：BIORESOURCE TECHNOLOGY 2016
- 关键词：3-Hydroxypropionate Acetyl-CoA carboxylase Malonyl-CoA reductase Corynebacterium glutamicum Escherichia coli
- L-CystEIne Production in Escherichia coli Based on Rational Metabolic Engineering and Modular Strategy被引量：43收藏
- 作者：Liu, Han Fang, Guochen Wu, Hui Li, Zhimin Ye, Qin
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol;China Natl Light Ind Council
- 来源：BIOTECHNOLOGY JOURNAL 2018
- 关键词：Escherichia coli L-cystEIne modular strategy rational metabolic engineering
- One-pot synthesis of glutathione by a two-enzyme cascade using a thermophilic ATP regeneration system被引量：43收藏
- 作者：Zhang, Xing Wu, Hui Huang, Bing Li, Zhimin Ye, Qin
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2017
- 关键词：Glutathione Bifunctional glutathione synthetase ATP regeneration Polyphosphate Polyphosphate kinase
- Biosynthesis of trans-4-hydroxyproline by recombinant strains of Corynebacterium glutamicum and Escherichia coli被引量：40收藏
- 作者：Yi, Yulan Sheng, Huakai Li, Zhimin Ye, Qin
- 机构：E China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomanufacturing
- 来源：BMC BIOTECHNOLOGY 2014
- 关键词：Trans-4-hydroxy-L-proline Recombinant Corynebacterium glutamicum Recombinant Escherichia coli Proline 4-hydroxylases
- Characterization of a new lysine decarboxylase from Aliivibrio salmonicida for cadaverine production at alkaline pH被引量：40收藏
- 作者：Kou, Fengyu Zhao, Jing Liu, Jiao Shen, Jie Ye, Qin Zheng, Ping Li, Zhimin Sun, Jibin Ma, Yanhe
- 机构： State Key Laboratory of Bioreactor Engineering; Key Laboratory of Systems Microbial Biotechnology; Tianjin Institute of Industrial Biotechnology
- 来源：Journal of Molecular Catalysis B: Enzymatic 2016
- 关键词：Alkalinity - Amino acids
- Enhanced 1,3-propanediol production by supply of organic acids and repeated fed-batch culture被引量：39收藏
- 作者：Xue, Xuedong Li, Wei Li, Zhimin Xia, Yuelan Ye, Qin
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2010
- 关键词：1,3-Propanediol Organic acid mixture Repeated fed-batch culture Klebsiella pneumoniae
- Succinic acid production and CO2 fixation using a metabolically engineered Escherichia coli in a bioreactor equipped with a self-inducing agitator被引量：35收藏
- 作者：Wu, Hui Li, Qing Li, Zhi-Min Ye, Qin
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresource Technology 2012
- 关键词：Escherichia coli - Bioconversion - Bioreactors - Ethanol - Fermentation
- Enhanced production of human epidermal growth factor under control of the phoA promoter by acetate-tolerant Escherichia coli DB15 in a chemically defined medium被引量：35收藏
- 作者：Zhang, Yanjun Li, Zhimin Ye, Qin
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Biotechnology and Bioprocess Engineering 2010
- 关键词：Chlorine compounds - Gene expression - Escherichia coli - Dissolved oxygen
- Improved succinic acid production in the anaerobic culture of an Escherichia coli pflB ldhA double mutant as a result of enhanced anaplerotic activities in the preceding aerobic culture被引量：35收藏
- 作者：Wu, Hui Li, Zhi-Min Zhou, Li Ye, Qin
- 机构： State Key Laboratory of Bioreactor Engineering; State Key Laboratory of Bioreactor Engineering
- 来源：Applied and Environmental Microbiology 2007
- 关键词：Cell culture - Enzyme activity - Genetic engineering - Glucose - Metabolism - Mutagenesis
- 人工神经网络在红霉素发酵过程状态预估中的应用被引量：34收藏
- 作者：黄明志 杭海峰 储炬 叶勤 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2000
- 关键词：神经网络 BP网络 RBF网络 红霉素 发酵 状态预估
- 摘要：探索了动态 BP网络和 RBF网络在红霉素发酵过程状态预估中的应用 ,比较了它们的收敛速度和学习能力。结果表明 ,BP网络和 RBF网络都具有相当好的学习能力 ,但 RBF网络的收敛速度更快。训练好的神经网络 ,在红霉素...
- 下载全文在线阅读
- Simultaneous production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol by a recombinant strain of Klebsiella pneumoniae被引量：34收藏
- 作者：Huang, Yanna Li, Zhimin Shimizu, Kazuyuki Ye, Qin
- 机构： State Key Laboratory of Bioreactor Engineering; Department of Bioscience and Bioinformatics
- 来源：Bioresource Technology 2012
- 关键词：Escherichia coli - Batch cell culture - Aldehydes - Cloning - Gene expression
- Enhanced anaerobic succinic acid production by Escherichia coli NZN111 aerobically grown on gluconeogenic carbon sources被引量：33收藏
- 作者：Wu, Hui Li, Zhi-min Zhou, Li Xie, Jingli Ye, Qin
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Enzyme and Microbial Technology 2009
- 关键词：Sodium bicarbonate - Enzymes - Molecular biology - Cells - Carbon - Glucose - Cytology
- Efficient production of l-menthol in a two-phase system with SDS using an immobilized bacillus subtilis esterase被引量：32收藏
- 作者：Pan, Jiang Dang, Ngoc-Duy Zheng, Gao-Wei Cheng, Bo Ye, Qin Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology; Sunflower Pharmaceutical Group Company Limited
- 来源：Bioresources and Bioprocessing 2014
- 关键词：Cloning - Crosslinking - Enantioselectivity - Enzymatic hydrolysis - Enzyme activity - Enzyme inhibition - Esters - Sulfur compounds
- Effects of geometrical design on hydrodynamic and mass transfer characteristics of a rectangular-column airlift bioreactor被引量：32收藏
- 作者：Kilonzo, Peter M. Margaritis, Argyrios Bergougnou, M.A. Yu, Juntang Ye, Qin
- 机构：Univ Western Ontario;E China Univ Sci & Technol
- 来源：BIOCHEMICAL ENGINEERING JOURNAL 2007
- 关键词：airlift bioreactor clearance gas holdup liquid velocity pressure drop mass transfer
- Crosslinking of enzyme coaggregate with polyethylenEImine: A simple and promising method for preparing stable biocatalyst of Serratia marcescens lipase被引量：31收藏
- 作者：Pan, Jiang Kong, Xu-Dong Li, Chun-Xiu Ye, Qin Xu, Jian-He Imanaka, Tadayuki
- 机构： State Key Laboratory of Bioreactor Engineering; Department of Biotechnology
- 来源：Journal of Molecular Catalysis B: Enzymatic 2011
- 关键词：Coprecipitation - Enzyme immobilization
- Production of succinate from simply purified crude glycerol by engineered Escherichia coli using two-stage fermentation被引量：31收藏
- 作者：Li, Qing Huang, Bing He, Qiaofei Lu, Jingxian Li, Xun Li, Zhimin Wu, Hui Ye, Qin
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol;China Natl Light Ind Council;Nanjing Forestry Univ
- 来源：BIORESOURCES AND BIOPROCESSING 2018
- 关键词：Escherichia coli Succinate Crude glycerol Activated carbon Two-stage fermentation
- Heterologous expression and characterization of a novel thermo-halotolerant endoglucanase Cel5H from Dictyoglomus thermophilum被引量：31收藏
- 作者：Shi, Runrun Li, Zhimin Ye, Qin Xu, Jianhe Liu, Yan
- 机构： State Key Laboratory of Bioreactor Engineering; Biosystems and Agricultural Engineering
- 来源：Bioresource Technology 2013
- 关键词：Cloning
- Co-production of 3-hydroxypropionic acid and 1,3-propanediol by KlebsEIlla pneumoniae expressing aldH under microaerobic conditions被引量：30收藏
- 作者：Huang, Yanna Li, Zhimin Shimizu, Kazuyuki Ye, Qin
- 机构： State Key Laboratory of Bioreactor Engineering; Department of Bioscience and Bioinformatics
- 来源：Bioresource Technology 2013
- 关键词：Batch cell culture
- Cultivation of recombinant Escherichia coli for secretory production of human epidermal growth factor under control of PL promoter被引量：30收藏
- 作者：Zhang, Haiyi Li, Zhimin Qian, Yue Zhang, Qian Du, Peng Gan, Renbao Ye, Qin
- 机构： State Key Laboratory of Bioreactor Engineering; Institute of Biochemistry and Cell Biology
- 来源：Enzyme and Microbial Technology 2007
- 关键词：Cell culture - Enzymes - Genes - Nutrition - Thermal effects
- Production of glutathione using a bifunctional enzyme encoded by gshF from Streptococcus thermophilus expressed in Escherichia coli被引量：30收藏
- 作者：Li, Wei Li, Zhimin Yang, Jianhua Ye, Qin
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Biotechnology 2011
- 关键词：Glucose - Amino acids - Cloning - Peptides - Yeast - Enzyme inhibition
- 啤酒酵母的通透性对ATP生产活性的影响被引量：29收藏
- 作者：段学辉 叶勤 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2000
- 关键词：啤酒酵母 细胞膜 ATP 通透性
- 摘要：利用甲苯、超声波和气流干燥等方法，对用于ＡＴＰ生产的啤酒酵母进行处理，考察了酵母细胞的通透性对其ＡＴＰ生产活性的影响。结果表明，腺苷（Ａｄｏ）转化率低于２０％ 的好气培养细胞（Ａ－细胞）经处理后，转化率提高到３０％ ～１...
- 下载全文在线阅读
- Glutathione production by recombinant Escherichia coli expressing bifunctional glutathione synthetase被引量：27收藏
- 作者：Wang, Dezheng Wang, Cheng Wu, Hui Li, Zhimin Ye, Qin
- 机构：E China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol
- 来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2016
- 关键词：Glutathione Escherichia coli Bifunctional glutathione synthetase One-strain system Energy regeneration
- Production and optimization of recombinant human augmenter of liver regeneration by Pichia pastoris被引量：26收藏
- 作者：Li, Zhimin Ping, Xubin Ye, Qin Huang, Xiudong Cao, Zhifang
- 机构： State Key Laboratory of Bioreactor Engineering; Shanghai Wanxing Bio-Pharmaceutical Co. Ltd
- 来源：Enzyme and Microbial Technology 2010
- 关键词：Fermentation - Yeast - Feeding
- Heterologous gshF gene expression in various vector systems in Escherichia coli for enhanced glutathione production被引量：25收藏
- 作者：Wang, Cheng Zhang, Jing Wu, Hui Li, Zhimin Ye, Qin
- 机构：E China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2015
- 关键词：Antioxidant Glutathione Escherichia coil Bifunctional glutathione synthetase Fed-batch fermentation
- Production of succinate by a pflB ldhA double mutant of Escherichia coli overexpressing malate dehydrogenase被引量：25收藏
- 作者：Wang, Wei Li, Zhimin Xie, Jingli Ye, Qin
- 机构：E China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2009
- 关键词：Escherichia coli Malate dehydrogenase Bicarbonate Succinic acid Two-stage culture
