魏巍 收藏
( 任职于 生物工程学院)
生物化学与分子生物学专业：硕士生导师、 生物化工专业：硕士生导师、 微生物学专业：硕士生导师 男，1982年出生，中共党员，博士，华东理工大学生物工程学院副教授，硕士生导师。2004年起开始分子克隆表达相关研究，2006年于华东理工大学生物反应器工程国家重点实验室开展生物化工专业博士相关研究，随后留在华东理工大学鲁华生物技术研究所继续从事博士期间的课题研究。自2004年起，多年来一直从事芽孢杆菌表达系统构建及微生物代谢途径、极端微生物开发及工业酶制剂等相关研究工作，在表达体系及微生物转化的相关研究领域具有扎实的基础。同时我们研究室开展极端菌微生物全基因... 详细>>
- Inactivation and Augmentation of the Primary 3-Ketosteroid-Δ1-Dehydrogenase in Mycobacterium neoaurum NwIB-01: Biotransformation of Soybean Phytosterols to 4-Androstene-3,17-Dione or 1,4-Androstadiene-3,17-Dione被引量：63收藏
- 作者：Wei, Wei Wang, Feng-qing Fan, Shu-yue Wei, Dong-zhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2010
- 质疑
- A novel cold-adapted type I pullulanase of Paenibacillus polymyxa Nws-pp2: in vivo functional expression and biochemical characterization of glucans hydrolyzates analysis被引量：31收藏
- 作者：Wei, Wei Ma, Jing Chen, Si-Qi Cai, Xiang-Hai Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：BMC BIOTECHNOLOGY 2015
- 关键词：Pullulanase type I Paenibacillus polymyxa Cold-adapted pullulanase Functional expression Enzymatic properties Starch hydrolysis products
- 质疑
- Gene cloning and characterization of ferritin H and M subunits from large yellow croaker (Pseudosciaena crocea)被引量：30收藏
- 作者：Zhang, Xiao Wei, Wei Wu, Haizhen Xu, Hongyan Chang, Kangmei Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Zhejiang Ocean Univ
- 来源：FISH & SHELLFISH IMMUNOLOGY 2010
- 关键词：Ferritin Immune-associated Large yellow croaker Attenuated live Vibrio anguillarum
- 质疑
- Accumulation of androstadiene-dione by overexpression of heterologous 3-ketosteroid Δ1-dehydrogenase in Mycobacterium neoaurum NwIB-01被引量：29收藏
- 作者：Wei, Wei Fan, Shu-Yue Wang, Feng-Qing Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 2014
- 关键词：1,4-Androstadiene-3,17-dione 3-Ketosteroid Delta(1)-dehydrogenase 4-Androstene-3,17-dione Gene overexpression Phytosterols Plasmid stability
- 质疑
- Autotransporter domain-dependent enzymatic analysis of a novel extremely thermostable carboxylesterase with high biodegradability towards pyrethroid pesticides被引量：28收藏
- 作者：Cai, Xianghai Wang, Wei Lin, Lin He, Dannong Huang, Gang Shen, Yaling Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Natl Engn Res Ctr Nanotechnol;Shanghai Univ Med & Hlth Sci
- 来源：SCIENTIFIC REPORTS 2017
- 质疑
- Inactivation and augmentation of the primary 3-Ketosteroid- δ1-Dehydrogenase in mycobacterium neoaurum NwIB-01: Biotransformation of soybean phytosterols to 4-androstene-3,17-dione or 1,4-androstadiene-3,17-dione被引量：27收藏
- 作者：Wei, Wei Wang, Feng-Qing Fan, Shu-Yue Wei, Dong-Zhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied and Environmental Microbiology 2010
- 关键词：Gene encoding - Polyols
- 质疑
- A type I pullulanase of Bacillus cereus Nws-bc5 screening from stinky tofu brine: Functional expression in Escherichia coli and Bacillus subtilis and enzyme characterization被引量：26收藏
- 作者：Wei, Wei Ma, Jing Guo, Su Wei, Dong-zhi
- 机构：E China Univ Sci & Technol
- 来源：PROCESS BIOCHEMISTRY 2014
- 关键词：Stinky tofu brine Strain screening Pullulanase Gene cloning Enzymatic properties Bacillus subtilis expression
- 质疑
- Functional expression of a novel alkaline-adapted lipase of Bacillus amyloliquefaciens from stinky tofu brine and development of immobilized enzyme for biodiesel production被引量：26收藏
- 作者：Cai, Xianghai Ma, Jing Wei, Dong-zhi Lin, Jin-ping Wei, Wei
- 机构：E China Univ Sci & Technol
- 来源：ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 2014
- 关键词：Bacillus amyloliquefaciens Lipase Gene cloning Functional expression Immobilization
- 质疑
- N-Terminal Domain Truncation and Domain Insertion-Based Engineering of a Novel Thermostable Type I Pullulanase from Geobacillus thermocatenulatus被引量：23收藏
- 作者：Li, Lingmeng Dong, Fengying Lin, Lin He, Dannong Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci;Natl Engn Res Ctr Nanotechnol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2018
- 关键词：Geobacillus thermocatenulatus pullulanase domain truncation and insertion maize starch
- 质疑
- A New Steroid-Transforming Strain of Mycobacterium neoaurum and Cloning of 3-Ketosteroid 9α-Hydroxylase in NwIB-01被引量：23收藏
- 作者：Wei, Wei Fan, Shuyue Wang, Fengqing Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2010
- 关键词：Mycobacterium neoaurum Extraction chromatography Screening Phytosterols 4-Androstene-3,17-dione (AD) 1,4-Androstadiene-3,17-dione (ADD) 3-Ketosteroid 9 alpha-hydroxylase (KSH)
- 质疑
- Identification of differentially expressed genes in large yellow croaker (Pseudosciaena crocea) induced by attenuated live Vibrio anguillarum被引量：17收藏
- 作者：Wei, Wei Xu, Hongyan Wang, Qiyao Zhang, Xiao Chang, Kangmei Wu, Changwen Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Zhejiang Ocean Univ
- 来源：AQUACULTURE 2009
- 关键词：Differentially expressed gene Immune-relevant gene Large yellow croaker Suppression subtractive hybridization (SSH) Vibrio anguillarum
- 质疑
- Biochemical Characterization of a Novel Thermostable Type I Pullulanase Produced Recombinantly in Bacillus subtilis被引量：16收藏
- 作者：Li, Lingmeng Dong, Fengying Lin, Lin He, Dannong Chen, Jingwen Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci;Natl Engn Res Ctr Nanotechnol;Univ South Carolina
- 来源：STARCH-STARKE 2018
- 关键词：Bacillus subtilis enzymatic properties expression Geobacillus kaustophilus pullulanase thermostable secretion expression
- 质疑
- Cloning and molecular characterization of two complement Bf/C2 genes in large yellow croaker (Pseudosciaena crocea)被引量：16收藏
- 作者：Wei, Wei Wu, Haizhen Xu, Hongyan Xu, Tao Zhang, Xiao Chang, Kangmei Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Zhejiang Ocean Univ
- 来源：FISH & SHELLFISH IMMUNOLOGY 2009
- 关键词：Complement factor B Complement C2 Large yellow croaker 3D structure Attenuated live V. anguillarum
- 质疑
- Construction of a Shuttle Vector for Protein Secretory Expression in Bacillus subtilis and the Application of the Mannanase Functional Heterologous Expression被引量：16收藏
- 作者：Guo, Su Tang, Jia-jie Wei, Dong-zhi Wei, Wei
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 2014
- 关键词：Bacillus subtilis Bacillus pumilus green fluorescent protein mannanase P43 promoter secretory expression
- 质疑
- A light-switchable bidirectional expression system in filamentous fungus Trichoderma reesei被引量：15收藏
- 作者：Zhang, Guoxiu Liu, Pei Wei, Wei Wang, Xuedong Wei, Dongzhi Wang, Wei
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2016
- 关键词：Trichoderma reesei Light-switchable system Heterologous protein Simultaneous expression Cellulase-free background
- 质疑
- Enhancement of ethyl (S)-4-chloro-3-hydroxybutanoate production at high substrate concentration by in situ resin adsorption被引量：14收藏
- 作者：Chen, Li-Feng Fan, Hai-Yang Zhang, Yi-Ping Wei, Wei Lin, Jin-Ping Wei, Dong-Zhi Wang, Hua-Lei
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2017
- 关键词：Ethyl (S)-4-chloro-3-hydroxybutanoate Carbonyl reductase Asymmetric reduction Product inhibition Resin adsorption
- 质疑
- Protopectinase production by Paenibacillus polymyxa Z6 and its application in pectin extraction from apple pomace被引量：14收藏
- 作者：Zhang, Jian Zhao, Li Gao, Bei Wei, Wei Wang, Hualei Xie, Jingli
- 机构：East China Univ Sci & Technol;SCICB
- 来源：JOURNAL OF FOOD PROCESSING AND PRESERVATION 2018
- 关键词：Fruits - Moisture - Energy utilization - Fermentation - Viscosity
- 质疑
- Synergistic Regulation of Metabolism by Ca2+/Reactive Oxygen Species in Penicillium brevicompactum Improves Production of Mycophenolic Acid and Investigation of the Ca2+ Channel被引量：14收藏
- 作者：Chen, Mianhui Wang, Jingjing Lin, Lin Xu, Xiangyang Wei, Wei Shen, Yaling Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Inst Technol;Natl Engn Res Ctr Nanotechnol;Zaozhuang Jie Nuo Enzyme Co Ltd
- 来源：ACS SYNTHETIC BIOLOGY 2022
- 关键词：mycophenolic acid (MPA) ROS calcium pigment Penicillium brevicompactum VHb
- 质疑
- Cinnamyl Esters Synthesis By Lipase-Catalyzed Transesterification in a Non-Aqueous System被引量：13收藏
- 作者：Cai, Xianghai Wang, Wei Lin, Lin He, Dannong Shen, Yaling Wei, Wei Wei, Dong-zhi
- 机构：East China Univ Sci & Technol;Natl Engn Res Ctr Nanotechnol;Shanghai Univ Med & Hlth Sci
- 来源：CATALYSIS LETTERS 2017
- 关键词：Enzymatic catalysis Cinnamyl esters Green chemistry
- 质疑
- In vivo functional expression of an extracellular Ca2+-independent Bacillus pumilus lipase in Bacillus subtilis WB800N被引量：13收藏
- 作者：Ma, Jing Ma, Yushu Wei, Wei Wei, Dong-zhi
- 机构：E China Univ Sci & Technol
- 来源：ANNALS OF MICROBIOLOGY 2015
- 关键词：Strain screening Lipase Gene cloning Enzymatic properties Bacillus subtilis expression
- 质疑
- Cloning and molecular characterization of lycC1INH genes in large yellow croaker (Pseudosciaena crocea)被引量：12收藏
- 作者：Wei, Wei Wu, Haizhen Xu, Hongyan Zhang, Xiao Chu, Teng Wu, Changwen Chang, Kangmei Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Zhejiang Ocean Univ
- 来源：FISH & SHELLFISH IMMUNOLOGY 2010
- 关键词：Large yellow croaker (Pseudosciana crocea) Vibrio anguillarum lycC1INH Immune response
- 质疑
- Enhanced Thermostability of Geobacillus stearothermophilus α-Amylase by Rational Design of Disulfide Bond and Application in Corn Starch Liquefaction and Bread Quality Improvement被引量：11收藏
- 作者：Zhu, Mengyu Zhai, Wenxin Song, Runfei Lin, Lin Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Inst Technol;Natl Engn Res Ctr Nanotechnol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2023
- 关键词：alpha-amylase disulfide bond thermostability force network corn starch liquefaction breadaging
- 质疑
- Low-Temperature Synthesized Well-Crystallized ZnO Microspheres for Photocatalysis被引量：11收藏
- 作者：Wei, Wei Lin, Lin Cai, Xianghai Li, Yiwen Yu, Zhiqiang Zhong, Jian
- 机构：E China Univ Sci & Technol;Natl Engn Res Ctr Nanotechnol;Shanghai Ocean Univ;Univ Calif San Diego;Southern Med Univ
- 来源：SCIENCE OF ADVANCED MATERIALS 2016
- 关键词：Photocatalysis ZnO Flower-Like Microsphere Nanocrystal
- 质疑
- Functional expression of a novel methanol-stable esterase from Geobacillus subterraneusDSM13552 for biocatalytic synthesis of cinnamyl acetate in a solvent-free system被引量：10收藏
- 作者：Cai, Xianghai Lin, Lin Shen, Yaling Wei, Wei Wei, Dong-zhi
- 机构：East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci;Natl Engn Res Ctr Nanotechnol
- 来源：BMC BIOTECHNOLOGY 2020
- 关键词：Geobacillus subterraneus Esterase Methanol-stable Biocatalysis Cinnamyl acetate
- 质疑
- Structural and biochemical properties of a novel pullulanase of Paenibacillus lautus DSM 3035被引量：10收藏
- 作者：Chen, Si-Qi Cai, Xiang-Hai Xie, Jing-Li Wei, Wei Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：STARCH-STARKE 2017
- 关键词：Enzymatic properties Functional expression Hydrolysis products Paenibacillus lautus Pullulanase type I
- 质疑
- A novel host-vector system for heterologous protein co-expression and purification in the Trichoderma reesei industrial strain RUT-C30被引量：9收藏
- 作者：Zhang, Lei Zhang, Suping Jiang, Xin Wei, Wei Wang, Wei Wei, Dongzhi
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol;Tianjin Hualida Biotechnol Co Ltd
- 来源：BIOTECHNOLOGY LETTERS 2016
- 关键词：Alkaline cellulose Co-expression Filamentous fungi Phosphinothricin xyn2 Promoter Trichoderma reesei
- 质疑
- Transesterification Synthesis of Chloramphenicol Esters with the Lipase from Bacillus amyloliquefaciens被引量：9收藏
- 作者：Dong, Fengying Li, Lingmeng Lin, Lin He, Dannong Chen, Jingwen Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Natl Engn Res Ctr Nanotechnol;Univ South Carolina
- 来源：MOLECULES 2017
- 关键词：enzymatic catalysis regioselectivity chloramphenicol esters green chemistry Bacillus amyloliquefaciens
- 质疑
- Mn2+ modulates the production of mycophenolic acid in Penicillium brevicompactum NRRL864 via reactive oxygen species signaling and the investigation of pb-pho被引量：8收藏
- 作者：Chen, Mianhui Shen, Yaling Lin, Lin Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Inst Technol;Natl Engn Res Ctr Nanotechnol
- 来源：FUNGAL BIOLOGY 2022
- 关键词：Manganese-calcium-ROS Penicillium brevicompactum Cell wall integrity Mycophenolic acid Heat shock
- 质疑
- Bioprospecting metagenomics of a microbial community on cotton degradation: Mining for new glycoside hydrolases被引量：8收藏
- 作者：Zhang, Guoxiu Liu, Pei Zhang, Lei Wei, Wei Wang, Xuedong Wei, Dongzhi Wang, Wei
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2016
- 关键词：Glycoside hydrolase Microbial community Metagenomics Crude cotton Expression
- 质疑
- Exploiting loop-grafting strategy resorting on computer-aided design to improve the thermostability of alpha-amylase from Geobacillus stearothermophilus被引量：8收藏
- 作者：Zhu, Mengyu Zhai, Wenxin Jiang, Haolin Lin, Lin Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Nanjing Univ Informat Sci & Technol;Shanghai Inst Technol;Natl Engn Res Ctr Nanotechnol
- 来源：PROCESS BIOCHEMISTRY 2023
- 关键词：Alpha-amylase Loop-grafting Molecular dynamic simulation Thermostability Corn starch liquefaction
- 质疑
