王学东 收藏
( 任职于 生物工程学院鲁华生物技术研究所)
生物化工专业：硕士生导师 生物工程专业：硕士生导师 生物工程领域：工程硕士导师 工学博士，副研究员，华东理工大学生物工程学院，生物反应器国家重点实验室固定研究人员，生物化工、发酵工程、生物工程专业硕士生导师。 曾在医药企业工作多年，主要从事抗生素发酵，生物、生化药物的生产与管理，以及药物的研发工作。现为华东理工大学生物工程学院以及生物反应器工程国家重点实验室工作人员，其间于2012年8月赴美国佐治亚理工化学与生物分子工程学院进行为期一年的访问交流。主要从事生物技术药物领域的研发和相关技术研究工作，着... 详细>>
- An improved 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay for evaluating the viability of Escherichia coli cells被引量：181收藏
- 作者：Wang, Hengwei Cheng, Hairong Wang, Fengqing Wei, Dongzhi Wang, Xuedong
- 机构：E China Univ Sci & Technol;Shanghai Jiao Tong Univ
- 来源：JOURNAL OF MICROBIOLOGICAL METHODS 2010
- 关键词：MTT reduction Formazan E. coli Viability
- 质疑
- Mixed bacteria-loaded biochar for the immobilization of arsenic, lead, and cadmium in a polluted soil system: Effects and mechanisms被引量：97收藏
- 作者：Ji, Xiaowen Wan, Jiang Wang, Xuedong Peng, Cheng Wang, Gehui Liang, Weiyu Zhang, Wei
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：SCIENCE OF THE TOTAL ENVIRONMENT 2022
- 关键词：Biochar Soil Heavy metals Mixed bacteria-loaded biochar Mechanisms
- 质疑
- Immobilization of Ni (Ⅱ) at three levels of contaminated soil by rhamnolipids modified nano zero valent iron (RL@nZVI): Effects and mechanisms被引量：59收藏
- 作者：Sang, Li Wang, Gehui Liu, Lin Bian, Hao Jiang, Lingling Wang, Huadong Zhang, Yinjie Zhang, Wei Peng, Cheng Wang, Xuedong
- 机构： State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process; Shanghai Institute of Pollution Control and Ecological Security; State Key Laboratory of Bioreactor Engineering
- 来源：Chemosphere 2021
- 关键词：Iron oxides - Surface active agents - Hydrogen bonds - Synthesis (chemical) - Remediation - Soil pollution - Soils - Carboxylation - Binary alloys
- 质疑
- Strategy for the improvement of prodigiosin production by a Serratia marcescens mutant through fed-batch fermentation被引量：45收藏
- 作者：Tao, JL Wang, XD Shen, YL Wei, DZ
- 机构：E China Univ Sci & Technol
- 来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 2005
- 关键词：feeding strategy mutation prodigiosin Serratia marcescens ultra violet
- 质疑
- development of an adsorption procedure for the direct separation and purification of prodigiosin from culture broth被引量：42收藏
- 作者：Wang, XD Tao, JL Wei, DZ Shen, YL Tong, WY
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 2004
- 关键词：adsorption resin chromatography fermentation in situ separation prodigiosin Serratia marcescens
- 质疑
- Immobilization of Ni (II) at three levels of contaminated soil by rhamnolipids modified nano zero valent iron (RL@nZVI): Effects and mechanisms被引量：40收藏
- 作者：Sang, Li Wang, Gehui Liu, Lin Bian, Hao Jiang, Lingling Wang, Huadong Zhang, Yinjie Zhang, Wei Peng, Cheng Wang, Xuedong
- 机构：East China Univ Sci & Technol;Shanghai Inst Pollut Control & Ecol Secur;East China Univ Sci & Technol
- 来源：CHEMOSPHERE 2021
- 关键词：Ni-contaminated soil Rhamnolipid nZVI Mechanisms
- 质疑
- Disparity in productive binding mode of the slow-reacting enantiomer determines the novel catalytic behavior of Candida antarctica lipase B被引量：33收藏
- 作者：Xu, Tao Zhang, Lujia Su, Erzheng Cui, Dongbing Wang, Xuedong Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Molecular Catalysis B: Enzymatic 2010
- 关键词：Candida - Enzymes - Yeast - Reaction kinetics - Molecular modeling - Binding energy - Enantioselectivity - Reaction intermediates - Substrates - Enantiomers
- 质疑
- Improving the mda-7/IL-24 refolding and purification process using optimized culture conditions based on the structure characteristics of inclusion bodies被引量：33收藏
- 作者：Wang, Xiaojuan Bai, Chaogang Zhang, Jian Sun, Aiyou Wang, Xuedong Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresources and Bioprocessing 2014
- 关键词：Cell death - Metal ions - Metals - Purification - Scanning electron microscopy - Solubility
- 质疑
- Oxygen vectors used for S-adenosylmethionine production in recombinant Pichia pastoris with sorbitol as supplemental carbon source被引量：29收藏
- 作者：Zhang, Jian-Guo Wang, Xue-Dong Zhang, Ji-Ning Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOSCIENCE AND BIOENGINEERING 2008
- 关键词：S-adenosylmethionine fermentation oxygen vectors Pichia pastoris supplemental carbon source
- 质疑
- 脂肪酶 RWL 催化合成二氢燕麦生物碱 D被引量：27收藏
- 作者：Chen, Jinbo Zhai, Chuntao Jin, Minrong Wu, Qian Yuan, Minjia Wang, Xuedong Ren, Yuhong Wang, Hualei
- 机构： School of Biotechnology; Shanghai Zhina Biotechnology Co.; Shanghai Qiran Biotechnology Co.; Shanghai Jinjia Technology Co.
- 来源：[1] School of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China; [2] Shanghai Zhina Biotechnology Co., Ltd., Shanghai, 201400, China; [3] Shanghai Qiran Biotechnology Co., Ltd., Shanghai, 201702, China; [4] Shanghai Jinjia Technology Co., Ltd., Shanghai, 201210, China 2026
- 质疑
- Oxygen vectors used for S-adenosylmethionine production in recombinant Pichia pastoris with sorbitol as supplemental carbon source被引量：24收藏
- 作者：Zhang, Jian-Guo Wang, Xue-Dong Zhang, Ji-Ning Wei, Dong-Zhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Bioscience and Bioengineering 2008
- 关键词：Drug products - Fermentation - Metabolism - Organic carbon - Oxygen - Production control
- 质疑
- Light-inducible genetic engineering and control of non-homologous end-joining in industrial eukaryotic microorganisms: LML 3.0 and OFN 1.0被引量：24收藏
- 作者：Zhang, Lei Zhao, Xihua Zhang, Guoxiu Zhang, Jiajia Wang, Xuedong Zhang, Suping Wang, Wei Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Jiangxi Normal Univ;E China Univ Sci & Technol
- 来源：SCIENTIFIC REPORTS 2016
- 质疑
- Enhanced Steroid Metabolites Production by Resting Cell Phytosterol Bioconversion被引量：24收藏
- 作者：Gao, X. -Q. Feng, J. -X. Wang, X. -D. Hua, Q. Wei, D. -Z.
- 机构：E China Univ Sci & Technol
- 来源：CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY 2015
- 关键词：phytosterol steroid metabolites resting cell bioconversion hydroxypropyl-beta-cyclodextrin optimization
- 质疑
- A new fermentation strategy for S-adenosylmethionine production in recombinant Pichia pastoris被引量：23收藏
- 作者：Zhang, Jianguo Wang, Xuedong Su, Erzheng Fang, Guochen Ren, Yuhong Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：BIOCHEMICAL ENGINEERING JOURNAL 2008
- 关键词：high cell density fermentation methanol consumption rate S-adenosylmethionine Pichia postoris
- 质疑
- Protein Engineering of a Nitrilase from Burkholderia cenocepacia J2315 for Efficient and Enantioselective Production of (R)-o-Chloromandelic Acid被引量：23收藏
- 作者：Wang, Hualei Gao, Wenyuan Sun, Huihui Chen, Lifeng Zhang, Lujia Wang, Xuedong Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2015
- 关键词：Hydrolysis - Genetic engineering - Biochemical engineering - Mutagenesis - Proteins
- 质疑
- Effect of organic cosolvent on kinetic resolution of tert-leucine by penicillin G acylase from Kluyvera citrophila被引量：22收藏
- 作者：Liu, SL Wei, DZ Song, QX Zhang, YW Wang, XD
- 机构：E China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2006
- 关键词：penicillin G acylase resolution organic cosolvent tert-leucine
- 质疑
- The influence of hydroxypropyl-β-cyclodextrin on the enantioselective hydrolysis of 2-amino phenylpropionitrile catalyzed by recombinant nitrilase被引量：21收藏
- 作者：Li, Ming-Yang Wang, Xue-Dong
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresources and Bioprocessing 2014
- 关键词：Amino acids - Biochemistry - Cyclodextrins - Hydrolysis - Hydrophobicity - Substrates
- 质疑
- Investigation of factors affecting biotransformation of phytosterols to 9-hydroxyandrost-4-ene-3,-17-dione based on the HP-β-CD-resting cells reaction system被引量：21收藏
- 作者：Gao, Xingqiang Feng, Jianxun Hua, Qiang Wei, Dongzhi Wang, Xuedong
- 机构：E China Univ Sci & Technol
- 来源：BIOCATALYSIS AND BIOTRANSFORMATION 2014
- 关键词：Biotransformation hydroxypropyl-beta-cyclodextrin Mycobacterium phytosterols rate limitation
- 质疑
- Mixed bacteria passivation for the remediation of arsenic, lead, and cadmium: Medium optimization and mechanisms被引量：20收藏
- 作者：Peng, Cheng Zhao, Xuan Ji, Xiaowen Wu, Jinhong Liang, Weiyu Song, Huihui Zhang, Wei Wang, Xuedong
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Shanghai Yaxin Urban Construction Co Ltd
- 来源：PROCESS SAFETY AND ENVIRONMENTAL PROTECTION 2023
- 关键词：Mixed bacteria Bacillus Optimization Heavy metal Bioremediation
- 质疑
- High purity recombinant human growth hormone (rhGH) expression in Escherichia coli under phoA promoter被引量：20收藏
- 作者：Song, Hao Jiang, Jingxin Wang, Xuedong Zhang, Jianguo
- 机构：East China Univ Sci & Technol;Univ Shanghai Sci & Technol
- 来源：BIOENGINEERED 2017
- 关键词：alkaline phosphatase Escherichia coli fed-batch fermentation strategy rhGH variant
- 质疑
- A new fermentation strategy for S-adenosylmethionine production in recombinant Pichia pastoris被引量：19收藏
- 作者：Zhang, Jianguo Wang, Xuedong Su, Erzheng Fang, Guochen Ren, Yuhong Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Biochemical Engineering Journal 2008
- 关键词：Cytology - Yeast - Fermentation - Bottles - Cells
- 质疑
- Effect of organic cosolvent on kinetic resolution of tert-leucine by penicillin G acylase from Kluyvera citrophila被引量：19收藏
- 作者：Liu, Shu-Lai Wei, Dong-Zhi Song, Qing-Xun Zhang, Ye-Wang Wang, Xue-Dong
- 机构： State Key Lab. of Bioreactor Engineering
- 来源：Bioprocess and Biosystems Engineering 2006
- 关键词：Enzymes - Hydrolysis - Organic solvents - Permittivity - Solutions
- 质疑
- High-level production of bovine enterokinase light chain using fed-batches by recombinant Pichia pastoris被引量：18收藏
- 作者：Zhang, J.-G. Wang, X.-D. Mao, X.-Z. Wei, D.-Z.
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Chemical and Biochemical Engineering Quarterly 2009
- 关键词：Bioreactors - Mammals - Cells - Chains - Cytology - Yeast
- 质疑
- A novel nitrilase from Rhodobacter sphaeroides LHS-305: cloning, heterologous expression and biochemical characterization被引量：17收藏
- 作者：Wang, Hualei Li, Guinan Li, Mingyang Wei, Dongzhi Wang, Xuedong
- 机构：E China Univ Sci & Technol
- 来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 2014
- 关键词：Nitrilase Rhodobacter sphaeroides 3-Cyanopyridine Substrate specificity Regioselectivity
- 质疑
- Reducing by-product formation in L-lactic acid fermentation by Rhizopus oryzae被引量：17收藏
- 作者：Wang, XD Sun, LJ Wei, DZ Wang, RM
- 机构：E China Univ Sci & Technol;Inst Shandong Light Ind
- 来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2005
- 关键词：fermentation fumaric acid L-lactic acid phosphate Rhizopus oryzae
- 质疑
- Preparation of optically pure tert-leucine by penicillin G acylase-catalyzed resolution被引量：17收藏
- 作者：Liu, SL Song, QX Wei, DZ Zhang, YW Wang, XD
- 机构：E China Univ Sci & Technol
- 来源：PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY 2006
- 关键词：hydrolysis Kluyvera citrophila penicillin G acylase preparation resolution tert-leucine
- 质疑
- Enhancing yield of S-adenosylmethionine in Pichia pastoris by controlling NH4+ concentration被引量：15收藏
- 作者：Zhang, Jianguo Wang, Xuedong Zheng, Yu Fang, Guochen Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2008
- 关键词：S-adenosylmethionine fed-batch fermentation NH4+ concentration Pichia pastoris
- 质疑
- A light-switchable bidirectional expression system in filamentous fungus Trichoderma reesei被引量：15收藏
- 作者：Zhang, Guoxiu Liu, Pei Wei, Wei Wang, Xuedong Wei, Dongzhi Wang, Wei
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2016
- 关键词：Trichoderma reesei Light-switchable system Heterologous protein Simultaneous expression Cellulase-free background
- 质疑
- Influence of temperature on nucleus degradation of 4-androstene-3, 17-dione in phytosterol biotransformation by Mycobacterium sp.被引量：14收藏
- 作者：Xu, X. W. Gao, X. Q. Feng, J. X. Wang, X. D. Wei, D. Z.
- 机构：E China Univ Sci & Technol
- 来源：LETTERS IN APPLIED MICROBIOLOGY 2015
- 关键词：3-ketosteroid-9-hydroxylase 3-ketosteroid-(1)-dehydrogenase AD and or ADD production biodegradation Mycobacterium phytosterol temperature effect
- 质疑
