- Effective cancer immunotherapy by Ganoderma lucidum polysaccharide-gold nanocomposites through dendritic cell activation and memory T cell response被引量：122收藏
- 作者：Zhang, Shulei Pang, Guibin Chen, Chao Qin, Jianzhong Yu, Huan Liu, Yongming Zhang, Xihui Song, Zhentao Zhao, Jian Wang, Fujun Wang, Yangyun Zhang, Leshuai W.
- 机构：Shanghai Univ Tradit Chinese Med;Soochow Univ;Changshu 2 Peoples Hosp;Soochow Univ;Zhejiang Reachall Pharmaceut Co Ltd
- 来源：CARBOHYDRATE POLYMERS 2019
- 关键词：Polysaccharides Dendritic cell Nanoparticle Immunoregulation Cancer therapy
- 质疑
- A general approach to the design of high-performance near-infrared (NIR) D-π-A type fluorescent dyes被引量：109收藏
- 作者：Luo, Xiao Li, Jin Zhao, Jie Gu, Luyan Qian, Xuhong Yang, Youjun
- 机构：East China Normal Univ;East China Univ Sci & Technol
- 来源：CHINESE CHEMICAL LETTERS 2019
- 关键词：Near infrared Polymethine cyanine Fluorescence Steric protection Rigidity
- 质疑
- Biochemical properties and potential applications of an organic solvent-tolerant lipase isolated from Serratia marcescens ECU1010被引量：84收藏
- 作者：Zhao, Li-Li Xu, Jian-He Zhao, Jian Pan, Jiang Wang, Zhi-Long
- 机构：E China Univ Sci & Technol;Shanghai Jiao Tong Univ
- 来源：PROCESS BIOCHEMISTRY 2008
- 关键词：lipase Serratia marcescens organic solvent tolerance enantiomeric resolution chiral compounds biochemical properties
- 质疑
- Comparison of immunoregulatory effects of polysaccharides from three natural herbs and cellular uptake in dendritic cells被引量：67收藏
- 作者：Zhu, Ning Lv, Xiaochen Wang, Yangyun Li, Jingling Liu, Yongming Lu, Wenfei Yang, Liecheng Zhao, Jian Wang, Fujun Zhang, Leshuai W.
- 机构：Shanghai Univ Tradit Chinese Med;Soochow Univ;Soochow Univ;Zhejiang Reachall Pharmaceut Co Ltd;East China Univ Sci & Technol
- 来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2016
- 关键词：Natural herbs Polysaccharides Dendritic cell Nitric oxide Endocytosis
- 质疑
- Enhanced cellulase production by decreasing intercellular pH through H+-ATPase gene deletion in Trichoderma reesei RUT-C30被引量：58收藏
- 作者：Liu, Pei Zhang, Guoxiu Chen, Yumeng Zhao, Jian Wang, Wei Wei, Dongzhi
- 机构： State Key Lab of Bioreactor Engineering
- 来源：Biotechnology for Biofuels 2019
- 关键词：Ammonia - Lignocellulose - Calcium - Carbon - Genes - Industrial chemicals
- 质疑
- Comparative studies on the immunoregulatory effects of three polysaccharides using high content imaging system被引量：53收藏
- 作者：Lv, Xiaocheng Chen, Dandan Yang, Liecheng Zhu, Ning Li, Jingling Zhao, Jian Hua, Zhibi Wang, Fu-Jun Zhang, Leshuai W.
- 机构：Shanghai Univ Tradit Chinese Med;Soochow Univ;Zhejiang Reachall Pharmaceut Co Ltd;E China Univ Sci & Technol;Shanghai R&D Ctr Standardizat Chinese Med
- 来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2016
- 关键词：Polysaccharide Natural products Immunoregulation Cell irregularity Nitrogen oxide Macrophage
- 质疑
- The protective role of autophagy in nephrotoxicity induced by bismuth nanoparticles through AMPK/mTOR pathway被引量：47收藏
- 作者：Liu, Yongming Yu, Huan Zhang, Xihui Wang, Yong Song, Zhentao Zhao, Jian Shi, Haibin Li, Ruibin Wang, Yangyun Zhang, Leshuai W.
- 机构：Soochow Univ;Soochow Univ;East China Univ Sci & Technol
- 来源：NANOTOXICOLOGY 2018
- 关键词：Bismuth CT imaging acute kidney injury autophagy cell signaling pathway
- 质疑
- An Unusual (R)-Selective Epoxide Hydrolase with High Activity for Facile Preparation of Enantiopure Glycidyl Ethers被引量：45收藏
- 作者：Zhao, Jing Chu, Yan-Yan Li, Ai-Tao Ju, Xin Kong, Xu-Dong Pan, Jiang Tang, Yun Xu, Jian-He
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol
- 来源：ADVANCED SYNTHESIS & CATALYSIS 2011
- 关键词：enantioselectivity epoxide hydrolase glycidyl ethers kinetic resolution molecular modeling
- 质疑
- Improving combination cancer immunotherapy by manipulating dual immunomodulatory signals with enzyme-triggered, cell-penetrating peptide-mediated biomodulators被引量：43收藏
- 作者：Pang, Guibin Chen, Piao Cao, Xuewei Yu, Huan Zhang, Leshuai W. Zhao, Jian Wang, Fu-Jun
- 机构： State Key Laboratory of Bioreactor Engineering; New Drug R&D Center; ECUST-FONOW Joint Research Center for Innovative Medicines; Institute of Chinese Materia Medica; School for Radiological and Interdisciplinary Sciences
- 来源：Biomaterials Science 2023
- 关键词：Antigen-antibody reactions - Cell proliferation - Chemical activation - Diseases - Macrophages - Peptides - Scaffolds (biology) - Tumors
- 质疑
- Biochemical properties and potential applications of an organic solvent-tolerant lipase isolated from Serratia marcescens ECU1010被引量：38收藏
- 作者：Zhao, Li-Li Xu, Jian-He Zhao, Jian Pan, Jiang Wang, Zhi-Long
- 机构： Laboratory of Biocatalysis and Bioprocessing; School of Pharmacy
- 来源：Process Biochemistry 2008
- 关键词：Bacteria - Chirality - Dimers - Enantiomers - Enzyme activity - Organic solvents
- 质疑
- Refolding, purification and characterization of an organic solvent-tolerant lipase from Serratia marcescens ECU1010被引量：37收藏
- 作者：Li, Suxia Pang, Huaiyu Lin, Kang Xu, Jianhe Zhao, Jian Fan, Liqiang
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Molecular Catalysis B: Enzymatic 2011
- 关键词：Cloning - Chromatography - Recombinant proteins - Purification - Organic solvents
- 质疑
- Expression and Characterization of a Novel Enantioselective Lipase from Aspergillus fumigatus被引量：34收藏
- 作者：Shangguan, Jiao-Jiao Fan, Li-Qiang Ju, Xin Zhu, Qing-Qing Wang, Fu-Jun Zhao, Jian Xu, Jian-He
- 机构： State Key Laboratory of Bioreactor Engineering; Institute of Chinese Materia Medica
- 来源：Applied Biochemistry and Biotechnology 2012
- 关键词：Aspergillus - Cloning - Fatty acids - Purification - Escherichia coli - Enantioselectivity
- 质疑
- Significantly improved expression and biochemical properties of recombinant serratia marcescens lipase as robust biocatalyst for kinetic resolution of chiral ester被引量：33收藏
- 作者：Wang, Yi Zhao, Jian Xu, Jian-He Fan, Li-Qiang Li, Su-Xia Zhao, Li-Li Mao, Xiao-Bo
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied Biochemistry and Biotechnology 2010
- 关键词：Esters - Gene expression - Recombinant proteins - Escherichia coli - Fatty acids - Affinity chromatography - Lipases - Substrates - Cloning - Metal ions - Metals - Nonionic surfactants - Purification
- 质疑
- Expression and Characterization of a Novel Lipase from Aspergillus fumigatus with High Specific Activity被引量：31收藏
- 作者：Shangguan, Jiao-Jiao Liu, Yu-Qiang Wang, Fu-Jun Zhao, Jian Fan, Li-Qiang Li, Su-Xia Xu, Jian-He
- 机构：E China Univ Sci & Technol;Shanghai Univ Chinese Tradit Med
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2011
- 关键词：Aspergillus fumigatus Recombinant lipase Expression Characterization Active site
- 质疑
- Enhancement of cellulase production in Trichoderma reesei RUT-C30 by comparative genomic screening被引量：31收藏
- 作者：Liu, Pei Lin, Aibo Zhang, Guoxiu Zhang, Jiajia Chen, Yumeng Shen, Tao Zhao, Jian Wei, Dongzhi Wang, Wei
- 机构：East China Univ Sci & Technol;Sunson Ind Grp Co Ltd
- 来源：MICROBIAL CELL FACTORIES 2019
- 关键词：Trichoderma reesei RUT-C30 Genome sequencing Cellulase production tre108642 tre56839 Alcohol dehydrogenase CRE1
- 质疑
- Heterologous overexpression of Vigna radiata epoxide hydrolase in Escherichia coli and its catalytic performance in enantioconvergent hydrolysis of p-nitrostyrene oxide into (R)-p-nitrophenyl glycol被引量：29收藏
- 作者：Zhu, Qing-Qing He, Wan-Hong Kong, Xu-Dong Fan, Li-Qiang Zhao, Jian Li, Su-Xia Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2014
- 关键词：RT-PCR Vigna radiata epoxide hydrolase p-Nitrostyrene oxide Enantioconvergency Characterization
- 质疑
- Switching a Newly Discovered Lactonase into an Efficient and Thermostable Phosphotriesterase by Simple Double Mutations His250Ile/Ile263Trp被引量：28收藏
- 作者：Luo, Xiao-Jing Kong, Xu-Dong Zhao, Jian Chen, Qi Zhou, Jiahai Xu, Jian-He
- 机构：E China Univ Sci & Technol;Chinese Acad Sci
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2014
- 关键词：organophosphate phosphotriesterase lactonase site-directed mutagenesis functional switch X-ray crystallography
- 质疑
- Effective Therapeutic Drug Delivery by GALA3, an Endosomal Escape Peptide with Reduced Hydrophobicity被引量：25收藏
- 作者：Li, Chen Cao, Xue-Wei Zhao, Jian Wang, Fu-Jun
- 机构：East China Univ Sci & Technol;Zhejiang Fonow Med Co Ltd;Shanghai R&D Ctr Standardizat Chinese Med;Shanghai Univ Tradit Chinese Med;East China Univ Sci & Technol
- 来源：JOURNAL OF MEMBRANE BIOLOGY 2020
- 关键词：Endosomal escape HBP GALA3 BLF1
- 质疑
- Combinatorial Evolution of Phosphotriesterase Toward a Robust Malathion Degrader by Hierarchical Iteration Mutagenesis被引量：25收藏
- 作者：Luo, Xiao-Jing Zhao, Jian Li, Chun-Xiu Bai, Yun-Peng Reetz, Manfred T. Yu, Hui-Lei Xu, Jian-He
- 机构：East China Univ Sci & Technol;Max Planck Inst Kohlenforsch;Philipps Univ Marburg
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2016
- 关键词：biocatalysis directed evolution DNA shuffling malathion phosphotriesterase
- 质疑
- Recombinant expression and purification of a MAP30-cell penetrating peptide fusion protein with higher anti-tumor bioactivity被引量：23收藏
- 作者：Lv, Qiang Yang, Xu-Zhong Fu, Long-Yun Lu, Yv-Ting Lu, Yan-Hua Zhao, Jian Wang, Fu-Jun
- 机构：E China Univ Sci & Technol;Zhejiang Reachall Pharmaceut Co Ltd;Shanghai Univ Chinese Tradit Med
- 来源：PROTEIN EXPRESSION AND PURIFICATION 2015
- 关键词：Recombinant Momordica Antiviral Protein 30 Kd (rMAP30) Ribosome inactivating protein Cell Penetrating Peptide Ni-NTA purification Anti-tumor activity
- 质疑
- Analysis of Triterpenoid Saponins Reveals Insights into Structural Features Associated with Potent Protein Drug Enhancement Effects被引量：22收藏
- 作者：Cao, Xue-Wei Wang, Fu-Jun Liew, Oi-Wah Lu, Ye-Zhou Zhao, Jian
- 机构：Zhejiang Fonow Med Co Ltd;Shanghai R&D Ctr Standardizat Chinese Med;Shanghai Univ Tradit Chinese Med;East China Univ Sci & Technol;Ctr Translat Med
- 来源：MOLECULAR PHARMACEUTICS 2020
- 关键词：saponins endosomal escape enhancers (EEEs) glycosylated triterpenoid ribosome-inactivating proteins (RIPS) cytosolic drug delivery drug release
- 质疑
- Engineering Escherichia coli for l-homoserine production被引量：22收藏
- 作者：Sun, Bing-Yao Wang, Feng-Qing Zhao, Jian Tao, Xin-Yi Liu, Min Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BASIC MICROBIOLOGY 2023
- 关键词：Escherichia coli hok sok system l-homoserine metabolic engineering plasmid stability
- 质疑
- A Monochromophoric Approach to Succinct Ratiometric Fluorescent Probes without Probe-Product Crosstalk被引量：22收藏
- 作者：Xin, Kai Li, Xinxing Guo, Yinghua Zhong, Youhuan Wang, Jungang Yang, Haotian Zhao, Jie Guo, Chunlei Huang, Yunxia Lei, Zuhai Ying, Yi-Lun Luo, Xiao Wang, Haolu Qian, Xuhong Yang, Wen Liang, Xiaowen Yang, Youjun
- 机构：East China Univ Sci & Technol;Changzheng Hosp;Shanghai Jiao Tong Univ;East China Univ Sci & Technol;Univ Queensland
- 来源：CCS CHEMISTRY 2021
- 关键词：ratiometric probe single chromophoric probe-product crosstalk cellular imaging total oxidative capacity
- 质疑
- A novel human derived cell-penetrating peptide in drug delivery被引量：20收藏
- 作者：Zhao, Jian Gao, Peng Xiao, Wei Fan, Li-Qiang Wang, Fu-Jun Li, Su-Xia Liu, Jian-Wen
- 机构：E China Univ Sci & Technol;Zhejiang Reachall Pharmaceut Co Ltd
- 来源：MOLECULAR BIOLOGY REPORTS 2011
- 关键词：hC-SOD3 Human cell-penetrating peptide Apoptin Drug delivery
- 质疑
- Recent research advancements on regioselective nitrilase: fundamental and applicative aspects被引量：19收藏
- 作者：Chen, Zhi Zhao, Jiang Jiang, Shuiqing Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2019
- 关键词：Biocatalysis Regioselective nitrilase Regioselectivity Molecular modifications Industrial applications
- 质疑
- Spectral and biodistributional engineering of deep near-infrared chromophore被引量：19收藏
- 作者：Dong, Yan Lu, Xicun Li, Yi Chen, Weichao Yin, Lei Zhao, Jie Hu, Xinru Li, Xinran Lei, Zuhai Wu, Yuyang Chen, Hao Luo, Xiao Qian, Xuhong Yang, Youjun
- 机构：East China Univ Sci & Technol;East China Normal Univ;Chinese Acad Sci
- 来源：CHINESE CHEMICAL LETTERS 2023
- 关键词：Near infrared fluorophore In vivo imaging Molecular engineering Biological distribution Angiography
- 质疑
- Gene cloning and expression of a novel hypoglycaemic peptide from Momordica charantia被引量：18收藏
- 作者：Wang, Bi-lian Zhang, Wen-ji Zhao, Jian Wang, Fu-jun Fan, Li-qiang Wu, Yi-xin Hu, Zhi-bi
- 机构：E China Univ Sci & Technol;Shanghai Univ Chinese Tradit Med;Hamamatsu Univ Sch Med
- 来源：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE 2011
- 关键词：Momordica charantia gene cloning polypeptide-P hypoglycaemic effect
- 质疑
- Structure-guided engineering of a flavin-containing monooxygenase for the efficient production of indirubin被引量：17收藏
- 作者：Sun, Bing-Yao Sui, Hua-Lu Liu, Zi-Wei Tao, Xin-Yi Gao, Bei Zhao, Ming Ma, Yu-Shu Zhao, Jian Liu, Min Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2022
- 关键词：Flavin-containing monooxygenase Indirubin Loop region Substrate tunnel Structure-guided enzyme engineering Microbial synthesis
- 质疑
- Significantly Improved Expression and Biochemical Properties of Recombinant Serratia marcescens Lipase as Robust Biocatalyst for Kinetic Resolution of Chiral Ester被引量：16收藏
- 作者：Wang, Yi Zhao, Jian Xu, Jian-He Fan, Li-Qiang Li, Su-Xia Zhao, Li-Li Mao, Xiao-Bo
- 机构：E China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2010
- 关键词：Serratia marcescens ECU1010 Lipase Soluble expression Purification Characterization Enantioselectivity Biocatalytic resolution
- 质疑
- Statistical Optimizing of Medium for Clavulanic Acid Production by Streptomyces clavuligerus Using Response Surface Methodology被引量：15收藏
- 作者：Feng, Tao Zhao, Jie Chu, Ju Wang, Yong-hong Zhuang, Ying-ping
- 机构：East China Univ Sci & Technol;Sinopharm Weiqida Pharmaceut Co Ltd
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2021
- 关键词：Clavulanic acid Streptomyces clavuligerus Statistical optimization Response surface methodology
- 质疑
