成果/Result
- A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules被引量：378收藏
- 作者：Liang, Mindong Li, Zilong Wang, Weishan Liu, Jiakun Liu, Leshi Zhu, Guoliang Karthik, Loganathan Wang, Man Wang, Ke-Feng Wang, Zhong Yu, Jing Shuai, Yuting Yu, Jiaming Zhang, Lu Yang, Zhiheng Li, Chuan Zhang, Qian Shi, Tong Zhou, Liming Xie, Feng Dai, Huanqin Liu, Xueting Zhang, Jingyu Liu, Guang Zhuo, Ying Zhang, Buchang Liu, Chenli Li, Shanshan Xia, Xuekui Tong, Yaojun Liu, Yanwen Alterovitz, Gil Tan, Gao-Yi Zhang, Li-Xin
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Anhui Univ;Chinese Acad Sci;Chinese Acad Sci
- 来源：NATURE COMMUNICATIONS 2019
- Harnessing the intracellular triacylglycerols for titer improvement of polyketides in Streptomyces被引量：143收藏
- 作者：Wang, Weishan Li, Shanshan Li, Zilong Zhang, Jingyu Fan, Keqiang Tan, Gaoyi Ai, Guomin Lam, Sin Man Shui, Guanghou Yang, Zhiheng Lu, Hongzhong Jin, Pinjiao Li, Yihong Chen, Xiangyin Xia, Xuekui Liu, Xueting Dannelly, H. Kathleen Yang, Chen Yang, Yi Zhang, Siliang Alterovitz, Gil Xiang, Wensheng Zhang, Lixin
- 机构：Chinese Acad Sci;East China Univ Sci & Technol;Chinese Acad Agr Sci;Chinese Acad Sci;Qilu Univ Technol
- 来源：NATURE BIOTECHNOLOGY 2020
- 关键词：Ketones - Metabolism - Biochemistry - Bacteria - Metabolites - Glycerol
- A versatile biosensing platform coupling CRISPR-Cas12a and aptamers for detection of diverse analytes被引量：88收藏
- 作者：Zhao, Xiangxiang Li, Shanshan Liu, Guang Wang, Zhong Yang, Zhiheng Zhang, Quanwei Liang, Mindong Liu, Jiakun Li, Zilong Tong, Yaojun Zhu, Guoliang Wang, Xinye Jiang, Lan Wang, Weishan Tan, Gao-Yi Zhang, Lixin
- 机构：East China Univ Sci & Technol ECUST;East China Univ Sci & Technol ECUST;Chinese Acad Agr Sci;Chinese Acad Sci;Shanghai Jiao Tong Univ
- 来源：SCIENCE BULLETIN 2021
- 关键词：CRISPR-Cas12a Aptamer Biosensing platform Diverse analyte Alpha fetoprotein detection Cocaine detection
- Heterologous Biosynthesis of Spinosad: An Omics-Guided Large, Polyketide Synthase Gene Cluster Reconstitution in Streptomyces被引量：82收藏
- 作者：Tan, Gao-Yi Deng, Kunhua Liu, Xinhua Tao, Hui Chang, Yingying Chen, Jia Chen, Kai Sheng, Zhi Deng, Zixin Liu, Tiangang
- 机构：Wuhan Univ;Wuhan Univ;Wuhan Inst Biotechnol;East China Univ Sci & Technol;Shenyang Res Inst Chem Ind Ltd Co
- 来源：ACS SYNTHETIC BIOLOGY 2017
- 关键词：spinosyn Streptornyces heterologous production metabolomics proteomics module optimization
- Rational synthetic pathway refactoring of natural products biosynthesis in actinobacteria被引量：55收藏
- 作者：Tan, Gao-Yi Liu, Tiangang
- 机构：Wuhan Univ;Wuhan Univ;Wuhan Inst Biotechnol;East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2017
- 关键词：Actinobacteria Natural product Synthetic pathway Refactoring Metabolic engineering
- Activating cryptic biosynthetic gene cluster through a CRISPR-Cas12a-mediated direct cloning approach被引量：54收藏
- 作者：Liang, Mindong Liu, Leshi Xu, Fei Zeng, Xiaoqian Wang, Runjun Yang, Jinling Wang, Weishan Karthik, Loganathan Liu, Jiakun Yang, Zhiheng Zhu, Guoliang Wang, Shuliu Bai, Linquan Tong, Yaojun Liu, Xueting Wu, Min Zhang, Li-Xin Tan, Gao-Yi
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Zhejiang Univ;Zhejiang Univ;Huzhou Univ
- 来源：NUCLEIC ACIDS RESEARCH 2022
- Polyketide pesticides from actinomycetes被引量：52收藏
- 作者：Li, Shanshan Yang, Bowen Tan, Gao-Yi Ouyang, Li-Ming Qiu, Shiwen Wang, Weishan Xiang, Wensheng Zhang, Lixin
- 机构：Chinese Acad Agr Sci;East China Univ Sci & Technol;Chinese Acad Sci;Northeast Agr Univ;Univ Chinese Acad Sci
- 来源：CURRENT OPINION IN BIOTECHNOLOGY 2021
- 关键词：Crop protection - Metabolites - Bacteria
- Harnessing a previously unidentified capability of bacterial allosteric transcription factors for sensing diverse small molecules in vitro被引量：42收藏
- 作者：Cao, Jiaqian Yao, Yongpeng Fan, Keqiang Tan, Gaoyi Xiang, Wensheng Xia, Xuekui Li, Shanshan Wang, Weishan Zhang, Lixin
- 机构：State Key Lab Microbial Resources;Chinese Acad Sci;East China Univ Sci & Technol;Chinese Acad Agr Sci;Qilu Univ Technol
- 来源：SCIENCE ADVANCES 2018
- 关键词：Biosensors - Transcription - DNA - Binding sites - Signal transduction
- High-yield porphyrin production through metabolic engineering and biocatalysis被引量：34收藏
- 作者：Chen, Haihong Wang, Yaohong Wang, Weishan Cao, Ting Zhang, Lu Wang, Zhengduo Chi, Xuran Shi, Tong Wang, Huangwei He, Xinwei Liang, Mindong Yang, Mengxue Jiang, Wenyi Lv, Dongyuan Yu, Jiaming Zhu, Guoliang Xie, Yongtao Gao, Bei Wang, Xinye Liu, Xueting Li, Youyuan Ouyang, Limin Zhang, Jingyu Liu, Huimin Li, Zilong Tong, Yaojun Xia, Xuekui Tan, Gao-Yi Zhang, Lixin
- 机构：East China Univ Sci & Technol;Chinese Acad Sci;Shanghai Jiao Tong Univ;Qilu Univ Technol
- 来源：NATURE BIOTECHNOLOGY 2025
- 关键词：Catalysis - Diagnosis - Fermentation - Medical applications - Metabolic engineering
- Learn from microbial intelligence for avermectins overproduction被引量：32收藏
- 作者：Gao, Qiang Tan, Gao-Yi Xia, Xuekui Zhang, Lixin
- 机构：East China Univ Sci & Technol;Tianjin Univ Sci & Technol;Shandong Acad Sci;Qingdao Natl Lab Marine Sci & Technol
- 来源：CURRENT OPINION IN BIOTECHNOLOGY 2017
- In vitro allosteric transcription factor-based biosensing被引量：31收藏
- 作者：Li, Shanshan Li, Zilong Tan, Gao-Yi Xin, Zhenguo Wang, Weishan
- 机构：Chinese Acad Agr Sci;Chinese Acad Sci;East China Univ Sci & Technol;East China Univ Sci & Technol;Univ Chinese Acad Sci
- 来源：TRENDS IN BIOTECHNOLOGY 2023
- 关键词：Analytic equipment - Molecules - Transcription - Transcription factors
- Systematic development of a highly efficient cell factory for 5-aminolevulinic acid production被引量：28收藏
- 作者：Zhou, Houming Zhang, Chengyu Li, Zilong Xia, Menglei Li, Zhenghong Wang, Zhengduo Tan, Gao-Yi Luo, Ying Zhang, Lixin Wang, Weishan
- 机构：East China Univ Sci & Technol;Chinese Acad Sci;Tianjin Univ Sci & Technol
- 来源：TRENDS IN BIOTECHNOLOGY 2024
- 关键词：Agriculture - Metabolic engineering - Metabolism
- Phosphate limitation increases coenzyme Q10 production in industrial Rhodobacter sphaeroides HY01被引量：25收藏
- 作者：Zhang, Lu Liu, Leshi Wang, Ke-Feng Xu, Lan Zhou, Liming Wang, Weishan Li, Chuan Xu, Zheng Shi, Tong Chen, Haihong Li, Yuanhang Xu, Hui Yang, XiuLiang Zhu, Zhichun Chen, Biqin Li, Dan Zhan, Guanghuang Zhang, Si-Liang Zhang, Li-Xin Tan, Gao-Yi
- 机构：ECUST;ECUST;Chinese Acad Sci;Chinese Acad Sci;Chinese Acad Sci
- 来源：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY 2019
- 关键词：R sphaeroides CoQ(10) Phosphate limitation Overproduction Scale-up Transcriptome
- Engineering thermophilic Geobacillus thermoglucosidasius for riboflavin production被引量：24收藏
- 作者：Yang, Zhiheng Sun, Qingqing Tan, Gaoyi Zhang, Quanwei Wang, Zhengduo Li, Chuan Qi, Fengxian Wang, Weishan Zhang, Lixin Li, Zilong
- 机构：East China Univ Sci & Technol;Chinese Acad Sci
- 来源：MICROBIAL BIOTECHNOLOGY 2021
- Integrating PCR-free amplification and synergistic sensing for ultrasensitive and rapid CRISPR/Cas12a-based SARS-CoV-2 antigen detection被引量：24收藏
- 作者：Zhao, Xiangxiang Wang, Zhengduo Yang, Bowen Li, Zilong Tong, Yaojun Bi, Yuhai Li, Zhenghong Xia, Xuekui Chen, Xiangyin Zhang, Lixin Wang, Weishan Tan, Gao-Yi
- 机构：East China Univ Sci & Technol ECUST;East China Univ Sci & Technol ECUST;Chinese Acad Sci;Shanghai Jiao Tong Univ;Shanghai Jiao Tong Univ
- 来源：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY 2021
- 关键词：CRISPR Cas12a Aptamer Antigen detection SARS-CoV-2 PCR-Free amplification Synergistic sensing
- A thermostable type I-B CRISPR-Cas system for orthogonal and multiplexed genetic engineering被引量：22收藏
- 作者：Yang, Zhiheng Li, Zilong Li, Bixiao Bu, Ruihong Tan, Gao-Yi Wang, Zhengduo Yan, Hao Xin, Zhenguo Zhang, Guojian Li, Ming Xiang, Hua Zhang, Lixin Wang, Weishan
- 机构：China Univ Sci & Technol ECUST;East China Univ Sci & Technol ECUST;Chinese Acad Sci;Ocean Univ China;Univ Chinese Acad Sci
- 来源：NATURE COMMUNICATIONS 2023
- Screening and engineering of high-activity promoter elements through transcriptomics and red fluorescent protein visualization in Rhodobacter sphaeroides被引量：19收藏
- 作者：Shi, Tong Zhang, Lu Liang, Mindong Wang, Weishan Wang, Kefeng Jiang, Yue Liu, Jing He, Xinwei Yang, Zhiheng Chen, Haihong Li, Chuan Lv, Dongyuan Zhou, Liming Chen, Biqin Li, Dan Zhang, Li-Xin Tan, Gao-Yi
- 机构：East China Univ Sci & Technol ECUST;East China Univ Sci & Technol ECUST;Chinese Acad Sci;Chinese Acad Sci;Inner Mongolia Kingdomway Pharmaceut Co Ltd
- 来源：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY 2021
- 关键词：Rhodobacter sphaeroides Promoter library Transcriptomics Co-enzyme Q 10 Red fluorescent protein
- Assessment of light distribution model for marine red microalga Porphyridium purpureum for sustainable production in photobioreactor被引量：17收藏
- 作者：Li, Shaohua Huang, Jianke Ji, Liang Chen, Cheng Wu, Ping Zhang, Wei Tan, Gaoyi Wu, Haizhen Fan, Jianhua
- 机构：East China Univ Sci & Technol;Hohai Univ;Hunan Inst Engn;East China Univ Sci & Technol
- 来源：ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 2021
- 关键词：Red algae Biomass productivity Sustainability production Light distribution model Volume-averaged light intensity
- Genome-guided investigation of anti-inflammatory sesterterpenoids with 5-15 trans-fused ring system from phytopathogenic fungi被引量：15收藏
- 作者：Jiang, Lan Zhu, Guoliang Han, Jianying Hou, Chengjian Zhang, Xue Wang, Zhixin Yuan, Weize Lv, Kangjie Cong, Zhanren Wang, Xinye Chen, Xiangyin Karthik, Loganathan Yang, Huanting Wang, Xuyuan Tan, Gaoyi Liu, Guang Zhao, Liya Xia, Xuekui Liu, Xiangyang Gao, Shushan Ma, Lei Liu, Mei Ren, Biao Dai, Huanqin Quinn, Ronald J. Hsiang, Tom Zhang, Jingyu Zhang, Lixin Liu, Xueting
- 机构：East China Univ Sci & Technol;Chinese Acad Sci;Griffith Univ;Qilu Univ Technol;T3 Biosci LLC
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2021
- 关键词：Fungi Bifunctional terpene synthases Terpenoids Anti-inflammatory
- Unleashing the potential: type I CRISPR-Cas systems in actinomycetes for genome editing被引量：13收藏
- 作者：Wang, Shuliu Zeng, Xiaoqian Jiang, Yue Wang, Weishan Bai, Linquan Lu, Yinhua Zhang, Lixin Tan, Gao-Yi
- 机构：East China Univ Sci & Technol ECUST;Chinese Acad Sci;Chinese Acad Sci;Shanghai Jiao Tong Univ;Shanghai Normal Univ
- 来源：NATURAL PRODUCT REPORTS 2024
- A rapid nucleic acid detection platform based on phosphorothioate-DNA and sulfur binding domain被引量：9收藏
- 作者：Shuai, Yuting Ju, Yi Li, Yuanhang Ma, Dini Jiang, Lan Zhang, Jingyu Tan, Gao-Yi Liu, Xueting Wang, Shenlin Zhang, Lixin Liu, Guang
- 机构：East China Univ Sci & Technol ECUST;East China Univ Sci & Technol ECUST;Shanghai Jiao Tong Univ
- 来源：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY 2023
- 关键词：Phosphorothioate Sulfur binding domain Nucleic acid detection
- Expanding horizons of CRISPR applications beyond genome editing被引量：6收藏
- 作者：Liang, Yu Tong, Shengkun Zhang, Jingyu Tan, Gao-Yi Zhang, Lixin Lee, Sang Yup Tong, Yaojun
- 机构：Shanghai Jiao Tong Univ;East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol SCI;Korea Adv Inst Sci & Technol KAIST;Korea Adv Inst Sci & Technol
- 来源：TRENDS IN GENETICS 2025
- Scalable secondary metabolite production in Streptomyces using a plug-and-play system被引量：5收藏
- 作者：Yang, Bowen Li, Zilong Zhang, Jingyu Qiu, Shiwen Liu, Xueting Liang, Zonglin Yan, Hao Zhang, Yanyan Liu, Lihong Xia, Bing Bao, Lianqun Li, Defeng Zhou, Shanshan Corre, Christophe Zhang, Chengyu Lu, Yinhua Tan, Gao-Yi Xia, Xuekui Li, Shanshan Zhang, Lixin Wang, Weishan
- 机构：East China Univ Sci & Technol ECUST;East China Univ Sci & Technol ECUST;Chinese Acad Sci;Beijing Key Lab Genet Element Biosourcing & Intell;Chinese Acad Agr Sci
- 来源：NATURE BIOTECHNOLOGY 2025
- 关键词：Biomolecules - Strain
- Engineering the Methylerythritol Phosphate Pathway and Using a Temporal Promoter for Enhanced Lycopene Production in Rhodobacter sphaeroides HY01被引量：3收藏
- 作者：He, Xinwei Wang, Dan Liu, Jing Shi, Tong Wang, Weishan Chen, Biqin Li, Dan Zhang, Lixin Tan, Gao-Yi
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Chinese Acad Sci;Inner Mongolia Kingdomway Pharmaceut Co Ltd;Shanghai Collaborat Innovat Ctr Biomfg Technol SCI
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2024
- 关键词：Rhodobactersphaeroides coenzyme Q(10) terpenoids lycopene MEP pathway temporal promoter
- Rational spatial rewiring of key enzymes enhances α-santalene production in Saccharomyces cerevisiae被引量：2收藏
- 作者：Tan, Wenwen Tong, Shengkun Gao, Yaojie Wang, Jing Zhang, Jingyu Xie, Zhiping Dai, Huanqin Liang, Yu Tan, Gao-Yi Zhang, Lixin Tong, Yaojun
- 机构：East China Univ Sci & Technol;Shanghai Jiao Tong Univ;Shanghai Collaborat Innovat Ctr Biomfg Technol SCI;Chinese Acad Sci;Univ Chinese Acad Sci
- 来源：BIORESOURCE TECHNOLOGY 2025
- 关键词：Subcellular enzyme localization Spatial engineering alpha-Santalene Saccharomyces cerevisiae Metabolic engineering Synthetic biology
- Model-Guided Systematic Metabolic Engineering for Enhanced Spinosad Biosynthesis in Saccharopolyspora spinosa NHF132被引量：1收藏
- 作者：Wang, Shuliu Liu, Yuxin Zhang, Qian Jiang, Yue Zeng, Xiaoqian Zhang, Chengyu Cheng, Jiagao Wang, Weishan Zhang, Lixin Tan, Gao-Yi
- 机构：East China Univ Sci & Technol ECUST;East China Univ Sci & Technol ECUST;East China Univ Sci & Technol;Chinese Acad Sci
- 来源：ADVANCED SCIENCE 2025
- 关键词：genome-scale metabolic model Saccharopolyspora spinosa secondary metabolite spinosad systems metabolic engineering
- High-yield porphyrin production through metabolic engineering and biocatalysis (05 Jun, 10.1038/s41587-024-02267-3, 2024)被引量：0收藏
- 作者：Chen, Haihong Wang, Yaohong Wang, Weishan Cao, Ting Zhang, Lu Wang, Zhengduo Chi, Xuran Shi, Tong Wang, Huangwei He, Xinwei Liang, Mindong Yang, Mengxue Jiang, Wenyi Lv, Dongyuan Yu, Jiaming Zhu, Guoliang Xie, Yongtao Gao, Bei Wang, Xinye Liu, Xueting Li, Youyuan Ouyang, Liming Zhang, Jingyu Liu, Huimin Li, Zilong Tong, Yaojun Xia, Xuekui Tan, Gao-Yi Zhang, Lixin
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Chinese Acad Sci;Shanghai Jiao Tong Univ;Qilu Univ Technol
- 来源：NATURE BIOTECHNOLOGY 2025
- Reporter systems in actinomycetes: Versatile tools for natural product discovery and production被引量：0收藏
- 作者：Jiang, Yue Liu, Yuxin Wang, Shuliu Zeng, Xiaoqian Lv, Dongyuan Tong, Yaojun Bai, Linquan Zhang, Lixin Tan, Gao-Yi
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Shanghai Jiao Tong 11 Univ
- 来源：BIOTECHNOLOGY ADVANCES 2026
- 关键词：Reporter system Actinomycetes Natural product
- DNA硫修饰结合蛋白识别序列的表征与拓展促进新型核酸检测技术发展被引量：0收藏
- 作者：帅玉婷 许安安 李甲乙 韩照晰 马蒂尼 段海容 王馨叶 蒋岚 张敬宇 谭高翼 刘雪婷 赵一雷 童垚俊 王申林 贺新义 邓子新 刘光 张立新
- 机构：East China Univ Sci & Technol ECUST;Shanghai Jiao Tong Univ
- 来源：《Science Bulletin》 2023
- 关键词：核酸检测技术 限制性核酸内切酶 SBD 序列模式 识别序列 防御系统 硫原子 抗氧化应激
- 摘要：DNA硫修饰是一种DNA磷酸骨架上的非桥接氧原子被硫原子所取代的修饰,属于原核生物表观遗传标志物之一,它对细菌防御系统、抗氧化应激和基因调控等生命过程具有重要影响.DNA硫修饰可以被硫修饰依赖限制性核酸内切酶的硫结合域S...
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- Schizochytrium limacinum valorizes volatile fatty acid-rich dark fermentative effluent for docosahexaenoic acid production被引量：0收藏
- 作者：Zhang, Yingqiu Chen, Cheng Wei, Kaixin Sun, Liyun Tan, Gaoyi Zhang, Xu Fan, Jianhua
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Shihezi Univ
- 来源：BIORESOURCE TECHNOLOGY 2025
- 关键词：Wastewater utilization Schizochytrium limacinum SR21 Butyric acid Biotransformation Fatty acid DHA
