成果/Result
- An improved 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay for evaluating the viability of Escherichia coli cells被引量：181收藏
- 作者：Wang, Hengwei Cheng, Hairong Wang, Fengqing Wei, Dongzhi Wang, Xuedong
- 机构：E China Univ Sci & Technol;Shanghai Jiao Tong Univ
- 来源：JOURNAL OF MICROBIOLOGICAL METHODS 2010
- 关键词：MTT reduction Formazan E. coli Viability
- Mixed bacteria-loaded biochar for the immobilization of arsenic, lead, and cadmium in a polluted soil system: Effects and mechanisms被引量：97收藏
- 作者：Ji, Xiaowen Wan, Jiang Wang, Xuedong Peng, Cheng Wang, Gehui Liang, Weiyu Zhang, Wei
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：SCIENCE OF THE TOTAL ENVIRONMENT 2022
- 关键词：Biochar Soil Heavy metals Mixed bacteria-loaded biochar Mechanisms
- Immobilization of Ni (Ⅱ) at three levels of contaminated soil by rhamnolipids modified nano zero valent iron (RL@nZVI): Effects and mechanisms被引量：59收藏
- 作者：Sang, Li Wang, Gehui Liu, Lin Bian, Hao Jiang, Lingling Wang, Huadong Zhang, Yinjie Zhang, Wei Peng, Cheng Wang, Xuedong
- 机构： State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process; Shanghai Institute of Pollution Control and Ecological Security; State Key Laboratory of Bioreactor Engineering
- 来源：Chemosphere 2021
- 关键词：Iron oxides - Surface active agents - Hydrogen bonds - Synthesis (chemical) - Remediation - Soil pollution - Soils - Carboxylation - Binary alloys
- Strategy for the improvement of prodigiosin production by a Serratia marcescens mutant through fed-batch fermentation被引量：45收藏
- 作者：Tao, JL Wang, XD Shen, YL Wei, DZ
- 机构：E China Univ Sci & Technol
- 来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 2005
- 关键词：feeding strategy mutation prodigiosin Serratia marcescens ultra violet
- development of an adsorption procedure for the direct separation and purification of prodigiosin from culture broth被引量：42收藏
- 作者：Wang, XD Tao, JL Wei, DZ Shen, YL Tong, WY
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 2004
- 关键词：adsorption resin chromatography fermentation in situ separation prodigiosin Serratia marcescens
- Immobilization of Ni (II) at three levels of contaminated soil by rhamnolipids modified nano zero valent iron (RL@nZVI): Effects and mechanisms被引量：40收藏
- 作者：Sang, Li Wang, Gehui Liu, Lin Bian, Hao Jiang, Lingling Wang, Huadong Zhang, Yinjie Zhang, Wei Peng, Cheng Wang, Xuedong
- 机构：East China Univ Sci & Technol;Shanghai Inst Pollut Control & Ecol Secur;East China Univ Sci & Technol
- 来源：CHEMOSPHERE 2021
- 关键词：Ni-contaminated soil Rhamnolipid nZVI Mechanisms
- Disparity in productive binding mode of the slow-reacting enantiomer determines the novel catalytic behavior of Candida antarctica lipase B被引量：33收藏
- 作者：Xu, Tao Zhang, Lujia Su, Erzheng Cui, Dongbing Wang, Xuedong Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Molecular Catalysis B: Enzymatic 2010
- 关键词：Candida - Enzymes - Yeast - Reaction kinetics - Molecular modeling - Binding energy - Enantioselectivity - Reaction intermediates - Substrates - Enantiomers
- Improving the mda-7/IL-24 refolding and purification process using optimized culture conditions based on the structure characteristics of inclusion bodies被引量：33收藏
- 作者：Wang, Xiaojuan Bai, Chaogang Zhang, Jian Sun, Aiyou Wang, Xuedong Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresources and Bioprocessing 2014
- 关键词：Cell death - Metal ions - Metals - Purification - Scanning electron microscopy - Solubility
- Oxygen vectors used for S-adenosylmethionine production in recombinant Pichia pastoris with sorbitol as supplemental carbon source被引量：29收藏
- 作者：Zhang, Jian-Guo Wang, Xue-Dong Zhang, Ji-Ning Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOSCIENCE AND BIOENGINEERING 2008
- 关键词：S-adenosylmethionine fermentation oxygen vectors Pichia pastoris supplemental carbon source
- 脂肪酶 RWL 催化合成二氢燕麦生物碱 D被引量：27收藏
- 作者：Chen, Jinbo Zhai, Chuntao Jin, Minrong Wu, Qian Yuan, Minjia Wang, Xuedong Ren, Yuhong Wang, Hualei
- 机构： School of Biotechnology; Shanghai Zhina Biotechnology Co.; Shanghai Qiran Biotechnology Co.; Shanghai Jinjia Technology Co.
- 来源：[1] School of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China; [2] Shanghai Zhina Biotechnology Co., Ltd., Shanghai, 201400, China; [3] Shanghai Qiran Biotechnology Co., Ltd., Shanghai, 201702, China; [4] Shanghai Jinjia Technology Co., Ltd., Shanghai, 201210, China 2026
- Oxygen vectors used for S-adenosylmethionine production in recombinant Pichia pastoris with sorbitol as supplemental carbon source被引量：24收藏
- 作者：Zhang, Jian-Guo Wang, Xue-Dong Zhang, Ji-Ning Wei, Dong-Zhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Bioscience and Bioengineering 2008
- 关键词：Drug products - Fermentation - Metabolism - Organic carbon - Oxygen - Production control
- Light-inducible genetic engineering and control of non-homologous end-joining in industrial eukaryotic microorganisms: LML 3.0 and OFN 1.0被引量：24收藏
- 作者：Zhang, Lei Zhao, Xihua Zhang, Guoxiu Zhang, Jiajia Wang, Xuedong Zhang, Suping Wang, Wei Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Jiangxi Normal Univ;E China Univ Sci & Technol
- 来源：SCIENTIFIC REPORTS 2016
- Enhanced Steroid Metabolites Production by Resting Cell Phytosterol Bioconversion被引量：24收藏
- 作者：Gao, X. -Q. Feng, J. -X. Wang, X. -D. Hua, Q. Wei, D. -Z.
- 机构：E China Univ Sci & Technol
- 来源：CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY 2015
- 关键词：phytosterol steroid metabolites resting cell bioconversion hydroxypropyl-beta-cyclodextrin optimization
- A new fermentation strategy for S-adenosylmethionine production in recombinant Pichia pastoris被引量：23收藏
- 作者：Zhang, Jianguo Wang, Xuedong Su, Erzheng Fang, Guochen Ren, Yuhong Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：BIOCHEMICAL ENGINEERING JOURNAL 2008
- 关键词：high cell density fermentation methanol consumption rate S-adenosylmethionine Pichia postoris
- Protein Engineering of a Nitrilase from Burkholderia cenocepacia J2315 for Efficient and Enantioselective Production of (R)-o-Chloromandelic Acid被引量：23收藏
- 作者：Wang, Hualei Gao, Wenyuan Sun, Huihui Chen, Lifeng Zhang, Lujia Wang, Xuedong Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2015
- 关键词：Hydrolysis - Genetic engineering - Biochemical engineering - Mutagenesis - Proteins
- Effect of organic cosolvent on kinetic resolution of tert-leucine by penicillin G acylase from Kluyvera citrophila被引量：22收藏
- 作者：Liu, SL Wei, DZ Song, QX Zhang, YW Wang, XD
- 机构：E China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2006
- 关键词：penicillin G acylase resolution organic cosolvent tert-leucine
- The influence of hydroxypropyl-β-cyclodextrin on the enantioselective hydrolysis of 2-amino phenylpropionitrile catalyzed by recombinant nitrilase被引量：21收藏
- 作者：Li, Ming-Yang Wang, Xue-Dong
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresources and Bioprocessing 2014
- 关键词：Amino acids - Biochemistry - Cyclodextrins - Hydrolysis - Hydrophobicity - Substrates
- Investigation of factors affecting biotransformation of phytosterols to 9-hydroxyandrost-4-ene-3,-17-dione based on the HP-β-CD-resting cells reaction system被引量：21收藏
- 作者：Gao, Xingqiang Feng, Jianxun Hua, Qiang Wei, Dongzhi Wang, Xuedong
- 机构：E China Univ Sci & Technol
- 来源：BIOCATALYSIS AND BIOTRANSFORMATION 2014
- 关键词：Biotransformation hydroxypropyl-beta-cyclodextrin Mycobacterium phytosterols rate limitation
- Mixed bacteria passivation for the remediation of arsenic, lead, and cadmium: Medium optimization and mechanisms被引量：20收藏
- 作者：Peng, Cheng Zhao, Xuan Ji, Xiaowen Wu, Jinhong Liang, Weiyu Song, Huihui Zhang, Wei Wang, Xuedong
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Shanghai Yaxin Urban Construction Co Ltd
- 来源：PROCESS SAFETY AND ENVIRONMENTAL PROTECTION 2023
- 关键词：Mixed bacteria Bacillus Optimization Heavy metal Bioremediation
- High purity recombinant human growth hormone (rhGH) expression in Escherichia coli under phoA promoter被引量：20收藏
- 作者：Song, Hao Jiang, Jingxin Wang, Xuedong Zhang, Jianguo
- 机构：East China Univ Sci & Technol;Univ Shanghai Sci & Technol
- 来源：BIOENGINEERED 2017
- 关键词：alkaline phosphatase Escherichia coli fed-batch fermentation strategy rhGH variant
- A new fermentation strategy for S-adenosylmethionine production in recombinant Pichia pastoris被引量：19收藏
- 作者：Zhang, Jianguo Wang, Xuedong Su, Erzheng Fang, Guochen Ren, Yuhong Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Biochemical Engineering Journal 2008
- 关键词：Cytology - Yeast - Fermentation - Bottles - Cells
- Effect of organic cosolvent on kinetic resolution of tert-leucine by penicillin G acylase from Kluyvera citrophila被引量：19收藏
- 作者：Liu, Shu-Lai Wei, Dong-Zhi Song, Qing-Xun Zhang, Ye-Wang Wang, Xue-Dong
- 机构： State Key Lab. of Bioreactor Engineering
- 来源：Bioprocess and Biosystems Engineering 2006
- 关键词：Enzymes - Hydrolysis - Organic solvents - Permittivity - Solutions
- High-level production of bovine enterokinase light chain using fed-batches by recombinant Pichia pastoris被引量：18收藏
- 作者：Zhang, J.-G. Wang, X.-D. Mao, X.-Z. Wei, D.-Z.
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Chemical and Biochemical Engineering Quarterly 2009
- 关键词：Bioreactors - Mammals - Cells - Chains - Cytology - Yeast
- A novel nitrilase from Rhodobacter sphaeroides LHS-305: cloning, heterologous expression and biochemical characterization被引量：17收藏
- 作者：Wang, Hualei Li, Guinan Li, Mingyang Wei, Dongzhi Wang, Xuedong
- 机构：E China Univ Sci & Technol
- 来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 2014
- 关键词：Nitrilase Rhodobacter sphaeroides 3-Cyanopyridine Substrate specificity Regioselectivity
- Reducing by-product formation in L-lactic acid fermentation by Rhizopus oryzae被引量：17收藏
- 作者：Wang, XD Sun, LJ Wei, DZ Wang, RM
- 机构：E China Univ Sci & Technol;Inst Shandong Light Ind
- 来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2005
- 关键词：fermentation fumaric acid L-lactic acid phosphate Rhizopus oryzae
- Preparation of optically pure tert-leucine by penicillin G acylase-catalyzed resolution被引量：17收藏
- 作者：Liu, SL Song, QX Wei, DZ Zhang, YW Wang, XD
- 机构：E China Univ Sci & Technol
- 来源：PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY 2006
- 关键词：hydrolysis Kluyvera citrophila penicillin G acylase preparation resolution tert-leucine
- Enhancing yield of S-adenosylmethionine in Pichia pastoris by controlling NH4+ concentration被引量：15收藏
- 作者：Zhang, Jianguo Wang, Xuedong Zheng, Yu Fang, Guochen Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2008
- 关键词：S-adenosylmethionine fed-batch fermentation NH4+ concentration Pichia pastoris
- A light-switchable bidirectional expression system in filamentous fungus Trichoderma reesei被引量：15收藏
- 作者：Zhang, Guoxiu Liu, Pei Wei, Wei Wang, Xuedong Wei, Dongzhi Wang, Wei
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2016
- 关键词：Trichoderma reesei Light-switchable system Heterologous protein Simultaneous expression Cellulase-free background
- Influence of temperature on nucleus degradation of 4-androstene-3, 17-dione in phytosterol biotransformation by Mycobacterium sp.被引量：14收藏
- 作者：Xu, X. W. Gao, X. Q. Feng, J. X. Wang, X. D. Wei, D. Z.
- 机构：E China Univ Sci & Technol
- 来源：LETTERS IN APPLIED MICROBIOLOGY 2015
- 关键词：3-ketosteroid-9-hydroxylase 3-ketosteroid-(1)-dehydrogenase AD and or ADD production biodegradation Mycobacterium phytosterol temperature effect
- 粘质沙雷氏菌产2,3-丁二醇培养基的优化被引量：13收藏
- 作者：李元芳 徐勤 曹学 王学东 沈亚领 魏东芝
- 机构：华东理工大学生物反应器工程国家重点实验室鲁华生物技术研究所;湖北轻工职业技术学院轻化工系
- 来源：《工业微生物》 2007
- 关键词：2,3-丁二醇 粘质沙雷氏菌 柠檬酸 培养基 响应面分析
- 摘要：研究了各种碳源、氮源、柠檬酸及无机盐对细胞生长与产物形成的影响,通过单因子、正交及中心组合设计响应面分析优化发酵培养基。结果表明在培养基中添加柠檬酸不但可以促进细胞生长与糖耗速度,还可以缩短发酵周期,提高2,3-丁二醇的...
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