郁惠蕾 收藏
( 任职于 生物工程学院)
生物化工专业：硕士生导师/博士生导师 生物工程专业：硕士生导师 生物工程领域：工程硕士生导师 郁惠蕾教授，2008年毕业于华东理工大学生物化工专业，获工学博士学位并获2010年优秀百篇博士论文提名奖。2010年起任华东理工大学生物反应器工程国家重点实验室副研究员，2017年晋升为教授。2012年赴日本短期学习嗜热微生物的培养，2013年受国家留学基金委资助赴MIT进修一年。针对酶催化的三要素——酶、底物和反应环境, 聚焦酶的底物适应性和逆境耐受性机制等关键科学问题, 着力开展生物催化剂创制和结构/功能调控方面的应用基础研究... 详细>>
- Immobilization of Serratia marcescens lipase onto amino-functionalized magnetic nanoparticles for repeated use in enzymatic synthesis of Diltiazem intermediate被引量：140收藏
- 作者：Hu, Bin Pan, Jiang Yu, Hui-Lei Liu, Jian-Wen Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：PROCESS BIOCHEMISTRY 2009
- 关键词：Lipase Immobilization Magnetic nanoparticles Enzymatic biocatalytic resolution Diltiazem intermediate Biphasic reaction
- 质疑
- Preparation of Structurally Diverse Chiral Alcohols by Engineering Ketoreductase CgKR1被引量：82收藏
- 作者：Zheng, Gao-Wei Liu, Yuan-Yang Chen, Qi Huang, Lei Yu, Hui-Lei Lou, Wen-Yong Li, Chun-Xiu Bai, Yun-Peng Li, Ai-Tao Xu, Jian-He
- 机构：East China Univ Sci & Technol;South China Univ Technol;Max Planck Inst Kohlenforsch
- 来源：ACS CATALYSIS 2017
- 关键词：asymmetric reduction ketoreductase chiral alcohol protein engineering substrate specificity biocatalysis
- 质疑
- Identification, purification and characterization of β-glucosidase from apple seed as a novel catalyst for synthesis of O-glucosides被引量：76收藏
- 作者：Yu, Hui-Lei Xu, Jian-He Lu, Wen-Ya Lin, Guo-Qiang
- 机构：E China Univ Sci & Technol;Chinese Acad Sci
- 来源：ENZYME AND MICROBIAL TECHNOLOGY 2007
- 关键词：apple seed characterization beta-glucosidase purification thermal stability
- 质疑
- Access to Optically Active Aryl Halohydrins Using a Substrate-Tolerant Carbonyl Reductase Discovered from Kluyveromyces thermotolerans被引量：71收藏
- 作者：Xu, Guo-Chao Yu, Hui-Lei Zhang, Xiao-Yan Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：ACS CATALYSIS 2012
- 关键词：carbonyl reductase asymmetric hydrogen transfer aryl halohydrin Kluyveromyces thermotolerans genome data mining substrate/product tolerance
- 质疑
- One-pot biocatalytic route from cycloalkanes to α,ω-dicarboxylic acids by designed Escherichia coli consortia被引量：68收藏
- 作者：Wang, Fei Zhao, Jing Li, Qian Yang, Jun Li, Renjie Min, Jian Yu, Xiaojuan Zheng, Gao-Wei Yu, Hui-Lei Zhai, Chao Acevedo-Rocha, Carlos G. Ma, Lixin Li, Aitao
- 机构：Hubei Univ;East China Univ Sci & Technol;East China Univ Sci & Technol;Biosyntia ApS
- 来源：NATURE COMMUNICATIONS 2020
- 质疑
- An engineered Pseudomonas putida can simultaneously degrade organophosphates, pyrethroids and carbamates被引量：64收藏
- 作者：Gong, Ting Xu, Xiaoqing Dang, Yulei Kong, Annie Wu, Yunbo Liang, Peixin Wang, Shufang Yu, Huilei Xu, Ping Yang, Chao
- 机构：Nankai Univ;Nankai Univ;East China Univ Sci & Technol;Shanghai Jiao Tong Univ
- 来源：SCIENCE OF THE TOTAL ENVIRONMENT 2018
- 关键词：Pesticide Bioremediation Metabolic engineering Pseudomonas putida KT2440
- 质疑
- Unusually Broad Substrate Profile of Self-Sufficient Cytochrome P450 Monooxygenase CYP116B4 from Labrenzia aggregata被引量：60收藏
- 作者：Yin, Yue-Cai Yu, Hui-Lei Luan, Zheng-Jiao Li, Ren-Jie Ouyang, Peng-Fei Liu, Jing Xu, Jian-He
- 机构：E China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：CHEMBIOCHEM 2014
- 关键词：cytochromes data mining hydroxylation oxidoreductases protein engineering substrate promiscuity
- 质疑
- Metabolic Engineering of Pseudomonas putida KT2440 for Complete Mineralization of Methyl Parathion and γ-Hexachlorocyclohexane被引量：60收藏
- 作者：Gong, Ting Liu, Ruihua Zuo, Zhenqiang Che, You Yu, Huilei Song, Cunjiang Yang, Chao
- 机构：Nankai Univ;Nankai Univ;E China Univ Sci & Technol
- 来源：ACS SYNTHETIC BIOLOGY 2016
- 关键词：methyl parathion gamma-hexachlorocyclohexane complete mineralization Pseudomonas putida KT2440
- 质疑
- Access to optically active aryl halohydrins using a substrate-tolerant carbonyl reductase discovered from kluyveromyces thermotolerans被引量：59收藏
- 作者：Xu, Guo-Chao Yu, Hui-Lei Zhang, Xiao-Yan Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology
- 来源：ACS Catalysis 2012
- 关键词：Data mining - Escherichia coli - Enzyme activity - Genes - Ketones
- 质疑
- Engineering of Cyclohexanone Monooxygenase for the Enantioselective Synthesis of (S)-Omeprazole被引量：59收藏
- 作者：Zhang, Yan Wu, Yin-Qi Xu, Na Zhao, Qian Yu, Hui-Lei Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Jiangsu Aosaikang Pharmaceut Co Ltd
- 来源：ACS SUSTAINABLE CHEMISTRY & ENGINEERING 2019
- 关键词：Biocatalysis Cyclohexanone monooxygenase Substrate specificity Protein engineering Esomeprazole sulfoxide Halo-based selection method
- 质疑
- Efficient production of (R)-o-chloromandelic acid by deracemization of o-chloromandelonitrile with a new nitrilase mined from Labrenzia aggregata被引量：53收藏
- 作者：Zhang, Chen-Sheng Zhang, Zhi-Jun Li, Chun-Xiu Yu, Hui-Lei Zheng, Gao-Wei Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2012
- 关键词：Nitrilase Labrenzia aggregate Enantioselective hydrolysis (R)-o-Chloromandelic acid Biphasic system
- 质疑
- A thermostable and organic-solvent tolerant esterase from Pseudomonas putida ECU1011: Catalytic properties and performance in kinetic resolution of α-hydroxy acids被引量：49收藏
- 作者：Ma, Bao-Di Yu, Hui-Lei Pan, Jiang Liu, Jia-Yan Ju, Xin Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：BIORESOURCE TECHNOLOGY 2013
- 关键词：Esterase Thermostability Organic-solvent tolerance alpha-Hydroxy (carboxylic) acids Deacetylation
- 质疑
- Enzymatic Production of l-Menthol by a High Substrate Concentration Tolerable Esterase from Newly Isolated Bacillus subtilis ECU0554被引量：48收藏
- 作者：Zheng, Gao-Wei Yu, Hui-Lei Zhang, Jian-Dong Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：ADVANCED SYNTHESIS & CATALYSIS 2009
- 关键词：Bacillus subtilis esterase enzyme catalysis high substrate concentration tolerance hydrolysis l-menthol dl-menthyl acetate
- 质疑
- 糖苷水解酶在糖苷合成中的应用概况被引量：47收藏
- 作者：郁惠蕾 许建和 林国强
- 机构：华东理工大学生物反应器工程国家重点实验室;中国科学院上海有机化学研究所
- 来源：《有机化学》 2006
- 关键词：糖苷酶 糖苷 合成 反应模式 生物反应器
- 摘要：糖苷是一类附加值很高的生理活性化合物;应用糖苷水解酶催化合成这类化合物具有很好的应用前景.综述了糖苷水解酶在催化寡糖、脂肪醇或芳香醇、多肽、萜类、酚类、生物碱以及抗生素等底物糖基化中的研究进展,着重说明了糖苷水解酶应用于...
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- Hydroxylases involved in terpenoid biosynthesis: a review被引量：47收藏
- 作者：Zhang, Zihan Wu, Qing-Yang Ge, Yue Huang, Zheng-Yu Hong, Ran Li, Aitao Xu, Jian-He Yu, Hui-Lei
- 机构：East China Univ Sci & Technol;Chinese Acad Sci;Hubei Univ
- 来源：BIORESOURCES AND BIOPROCESSING 2023
- 关键词：Biooxidation Cytochrome P450 monooxygenase Hydroxylase Natural products Terpenoids
- 质疑
- Enzymatic Preparation of the Chiral (S)-Sulfoxide Drug Esomeprazole at Pilot-Scale Levels被引量：45收藏
- 作者：Xu, Na Zhu, Jun Wu, Yin-Qi Zhang, Yan Xia, Jian-Ye Zhao, Qian Lin, Guo-Qiang Yu, Hui-Lei Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Jiangsu Aosaikang Pharmaceut Co Ltd
- 来源：ORGANIC PROCESS RESEARCH & DEVELOPMENT 2020
- 关键词：prazole sulfide monooxygenase esomeprazole asymmetric oxidation oxygen mass transfer reaction regulation
- 质疑
- Regioselectivity Engineering of Epoxide Hydrolase: Near-Perfect Enantioconvergence through a Single Site Mutation被引量：41收藏
- 作者：Li, Fu-Long Kong, Xu-Dong Chen, Qi Zheng, Yu-Cong Xu, Qin Chen, Fei-Fei Fan, Li-Qiang Lin, Guo-Qiang Zhou, Jiahai Yu, Hui-Lei Xu, Jian-He
- 机构：East China Univ Sci & Technol;Chinese Acad Sci;Shanghai Jiao Tong Univ;Shanghai Jiao Tong Univ
- 来源：ACS CATALYSIS 2018
- 关键词：biocatalysis epoxide hydrolase crystal structure regioselectivity enantioconvergence protein engineering
- 质疑
- Confining Enzyme Clusters in Bacteriophage P22 Enhances Cofactor Recycling and Stereoselectivity for Chiral Alcohol Synthesis被引量：40收藏
- 作者：Zhang, Yan-Qing Feng, Tao-Tao Cao, Yu-Fei Zhang, Xiao-Yan Wang, Tao Nina, Mario Roque Huanca Wang, Li-Cheng Yu, Hui-Lei Xu, Jian-He Ge, Jun Bai, Yun-Peng
- 机构：East China Univ Sci & Technol ECUST;Tsinghua Univ;Tsinghua Shenzhen Int Grad Sch;Shenzhen Bay Lab;Tsinghua Univ
- 来源：ACS CATALYSIS 2021
- 关键词：enzyme cluster enzymatic catalysis cofactor recycling confinement effects asymmetric reduction
- 质疑
- Increased Catalyst Productivity in α-Hydroxy Acids Resolution by Esterase Mutation and Substrate Modification被引量：39收藏
- 作者：Ma, Bao-Di Kong, Xu-Dong Yu, Hui-Lei Zhang, Zhi-Jun Dou, Shuai Xu, Yan-Peng Ni, Yan Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：ACS CATALYSIS 2014
- 关键词：catalyst productivity deacylation enzymatic resolution alpha-hydroxy acid product:catalyst ratio semirational design
- 质疑
- Synthesis of optically pure S-sulfoxide by Escherichia coli transformant cells coexpressing the P450 monooxygenase and glucose dehydrogenase genes被引量：38收藏
- 作者：Zhang, Jian-Dong Li, Ai-Tao Yu, Hui-Lei Imanaka, Tadayuki Xu, Jian-He
- 机构：E China Univ Sci & Technol;Ritsumeikan Univ
- 来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2011
- 关键词：Coexpression P450SMO Glucose dehydrogenase NADPH regeneration D-glucose Sulfoxidation
- 质疑
- Bioproduction of chiral mandelate by enantioselective deacylation of α-acetoxyphenylacetic acid using whole cells of newly isolated Pseudomonas sp ECU1011被引量：38收藏
- 作者：Ju, Xin Yu, Hui-Lei Pan, Jiang Wei, Dong-Zhi Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2010
- 关键词：Microbial esterase Pseudomonas sp. Enantioselective deacylation alpha-Acetoxyphenylacetic acid Enantiopure mandelic acid
- 质疑
- Efficient Synthesis of (R)-2-Chloro-1-(2,4-dichlorophenyl)ethanol with a Ketoreductase from Scheffersomyces stipitis CBS 6045被引量：38收藏
- 作者：Shang, Yue-Peng Chen, Qi Kong, Xu-Dong Zhang, Yu-Jun Xu, Jian-He Yu, Hui-Lei
- 机构：East China Univ Sci & Technol
- 来源：ADVANCED SYNTHESIS & CATALYSIS 2017
- 关键词：biocatalysis bioreduction (R)-2-chloro-1-(2,4-dichlorophenyl) ethanol cofactor recycle reductase
- 质疑
- Enhanced limonene production by optimizing the expression of limonene biosynthesis and MEP pathway genes in E. Coli被引量：38收藏
- 作者：Du, Fu-Liang Yu, Hui-Lei Xu, Jian-He Li, Chun-Xiu
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology
- 来源：Bioresources and Bioprocessing 2014
- 关键词：Biochemistry - Biosynthesis - Flavor compounds - Gene expression - Monoterpenes
- 质疑
- Combinatorial metabolic engineering of Pseudomonas putida KT2440 for efficient mineralization of 1,2,3-trichloropropane被引量：37收藏
- 作者：Gong, Ting Xu, Xiaoqing Che, You Liu, Ruihua Gao, Weixia Zhao, Fengjie Yu, Huilei Liang, Jingnan Xu, Ping Song, Cunjiang Yang, Chao
- 机构：Nankai Univ;Nankai Univ;East China Univ Sci & Technol;Chinese Acad Sci;Shanghai Jiao Tong Univ
- 来源：SCIENTIFIC REPORTS 2017
- 质疑
- Engineering Pseudomonas putida KT2440 for simultaneous degradation of carbofuran and chlorpyrifos被引量：37收藏
- 作者：Gong, Ting Liu, Ruihua Che, You Xu, Xiaoqing Zhao, Fengjie Yu, Huilei Song, Cunjiang Liu, Yanping Yang, Chao
- 机构：Nankai Univ;Nankai Univ;East China Univ Sci & Technol;Tianjin Med Univ
- 来源：MICROBIAL BIOTECHNOLOGY 2016
- 质疑
- Enantiocomplementary decarboxylative hydroxylation combining photocatalysis and whole-cell biocatalysis in a one-pot cascade process被引量：36收藏
- 作者：Xu, Jian Arkin, Mamatjan Peng, Yongzhen Xu, Weihua Yu, Huilei Lin, Xianfu Wu, Qi
- 机构：Zhejiang Univ;East China Univ Sci & Technol
- 来源：GREEN CHEMISTRY 2019
- 质疑
- Engineering Streptomyces coelicolor Carbonyl Reductase for Efficient Atorvastatin Precursor Synthesis被引量：36收藏
- 作者：Li, Min Zhang, Zhi-Jun Kong, Xu-Dong Yu, Hui-Lei Zhou, Jiahai Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Chinese Acad Sci
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2017
- 关键词：carbonyl reductase protein engineering catalytic activity thermostability ethyl-(S)-4-chloro-3-hydroxybutyrate
- 质疑
- Immobilization of glycolate oxidase from Medicago falcata on magnetic nanoparticles for application in biosynthesis of glyoxylic acid被引量：34收藏
- 作者：Zhu, Hong Pan, Jiang Hu, Bin Yu, Hui-Lei Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Bioprocessing
- 来源：Journal of Molecular Catalysis B: Enzymatic 2009
- 关键词：Nanomagnetics - Nanoparticles - Fourier transform infrared spectroscopy - Batch reactors - Enzyme immobilization - Enzyme activity - Synthesis (chemical) - Biochemistry - Plants (botany) - Scanning electron microscopy
- 质疑
- Dehydrogenase-Catalyzed Oxidation of Furanics: Exploitation of Hemoglobin Catalytic Promiscuity被引量：33收藏
- 作者：Jia, Hao-Yu Zong, Min-Hua Yu, Hui-Lei Li, Ning
- 机构：South China Univ Technol;East China Univ Sci & Technol
- 来源：CHEMSUSCHEM 2017
- 关键词：cofactor regeneration enzyme catalysis enzyme promiscuity oxidation platform chemicals
- 质疑
- A Baeyer-Villiger monooxygenase from Cupriavidus basilensis catalyzes asymmetric synthesis of (R)-lansoprazole and other pharmaco-sulfoxides被引量：33收藏
- 作者：Liu, Feng Shou, Chao Geng, Qiang Zhao, Chen Xu, Jianhe Yu, Huilei
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2021
- 关键词：Baeyer-Villiger monooxygenase asymmetric sulfoxidation (R)-lansoprazole proton pump inhibitor
- 质疑
