李春秀 收藏
( 任职于 生物工程学院)
生物工程专业：硕士生导师 生物化学与分子生物学专业：硕士生导师 2005年毕业于中国林科院林木分子生物学专业获得博士学位，2005年-2008年在中国林科院林木分子遗传重点实验室进行博士后研究。2012年赴日本短期学习嗜热微生物的培养。现于华东理工大学应用生物学系任职副教授，主讲仿生学、生态学、生物化学等课程; 同时也是生物催化与合成生物技术研究室的主要成员之一，从事新酶的资源挖掘和分子改造研究。主持承担国家自然基金、863计划子课题、上海市科委项目及企业合作等科... 详细>>
- Effects of exogenous GABA on gene expression of Caragana intermedia roots under NaCl stress: regulatory roles for H2O2 and ethylene production被引量：152收藏
- 作者：Shi, Sheng-Qing Shi, Zheng Jiang, Ze-Ping Qi, Li-Wang Sun, Xiao-Mei Li, Chun-Xiu Liu, Jian-Feng Xiao, Wen-Fa Zhang, Shou-Gong
- 机构：Chinese Acad Forestry;Chinese Acad Forestry;E China Univ Sci & Technol
- 来源：PLANT CELL AND ENVIRONMENT 2010
- 关键词：suppression subtractive hybridization gene expression signal transduction reactive oxygen species nitrogen sources proline
- 质疑
- Highly efficient synthesis of chiral alcohols with a novel NADH-dependent reductase from Streptomyces coelicolor被引量：129收藏
- 作者：Wang, Li-Juan Li, Chun-Xiu Ni, Yan Zhang, Jie Liu, Xiang Xu, Jian-He
- 机构：E China Univ Sci & Technol;Jiangnan Univ
- 来源：BIORESOURCE TECHNOLOGY 2011
- 关键词：Carbonyl reductase Streptomyces coelicolor Asymmetric reduction Ethyl (S)-4-chloro-3-hydroxybutanoate NADH regeneration
- 质疑
- Reshaping the Active Pocket of Amine Dehydrogenases for Asymmetric Synthesis of Bulky Aliphatic Amines被引量：102收藏
- 作者：Chen, Fei-Fei Zheng, Gao-Wei Liu, Lei Li, Hao Chen, Qi Li, Fu-Long Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol
- 来源：ACS CATALYSIS 2018
- 关键词：amine dehydrogenase reductive amination chiral amine protein engineering biocatalysis substrate scope
- 质疑
- Preparation of Structurally Diverse Chiral Alcohols by Engineering Ketoreductase CgKR1被引量：82收藏
- 作者：Zheng, Gao-Wei Liu, Yuan-Yang Chen, Qi Huang, Lei Yu, Hui-Lei Lou, Wen-Yong Li, Chun-Xiu Bai, Yun-Peng Li, Ai-Tao Xu, Jian-He
- 机构：East China Univ Sci & Technol;South China Univ Technol;Max Planck Inst Kohlenforsch
- 来源：ACS CATALYSIS 2017
- 关键词：asymmetric reduction ketoreductase chiral alcohol protein engineering substrate specificity biocatalysis
- 质疑
- Biocatalytic properties of a recombinant aldo-keto reductase with broad substrate spectrum and excellent stereoselectivity被引量：68收藏
- 作者：Ni, Yan Li, Chun-Xiu Ma, Hong-Min Zhang, Jie Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2011
- 关键词：YtbE Recombinant aldo-keto reductase Bacillus sp. Asymmetric reduction Chiral alcohols
- 质疑
- Two-step enzymatic synthesis of ursodeoxycholic acid with a new 7β-hydroxysteroid dehydrogenase from Ruminococcus torques被引量：66收藏
- 作者：Zheng, Ming-Min Wang, Ru-Feng Li, Chun-Xiu Xu, Jian-He
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol;China Pharmaceut Univ
- 来源：PROCESS BIOCHEMISTRY 2015
- 关键词：Ursodeoxycholic acid Chenodeoxycholic acid 7 alpha-Hydroxysteroid dehydrogenase 7 beta-Hydroxysteroid dehydrogenase Two-step reaction
- 质疑
- Switching Cofactor Dependence of 7β-Hydroxysteroid Dehydrogenase for Cost-Effective Production of Ursodeoxycholic Acid被引量：66收藏
- 作者：You, Zhi-Neng Chen, Qi Shi, Shou-Cheng Zheng, Ming-Min Pan, Jiang Qian, Xiao-Long Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Suzhou Bioforany EnzyTech Co Ltd
- 来源：ACS CATALYSIS 2019
- 关键词：biocatalysis cofactor dependence small-and-smart library 7 beta-hydroxysteroid dehydrogenase molecular dynamics simulation ursodeoxycholic acid
- 质疑
- Efficient Synthesis of a Chiral Precursor for Angiotensin-Converting Enzyme (ACE) Inhibitors in High Space-Time Yield by a New Reductase without External Cofactors被引量：61收藏
- 作者：Shen, Nai-Dong Ni, Yan Ma, Hong-Min Wang, Li-Juan Li, Chun-Xiu Zheng, Gao-Wei Zhang, Jie Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：ORGANIC LETTERS 2012
- 质疑
- Stereospecific Reduction of Methyl o-Chlorobenzoylformate at 300 g?L-1 without Additional Cofactor using a Carbonyl Reductase Mined from Candida glabrata被引量：57收藏
- 作者：Ma, Hongmin Yang, Linlin Ni, Yan Zhang, Jie Li, Chun-Xiu Zheng, Gao-Wei Yang, Huaiyu Xu, Jian-He
- 机构：E China Univ Sci & Technol;Chinese Acad Sci
- 来源：ADVANCED SYNTHESIS & CATALYSIS 2012
- 关键词：bioinformatics biotransformations cofactors oxidoreductases
- 质疑
- Engineering 7β-Hydroxysteroid Dehydrogenase for Enhanced Ursodeoxycholic Acid Production by Multiobjective Directed Evolution被引量：57收藏
- 作者：Zheng, Ming-Min Chen, Ke-Cai Wang, Ru-Feng Li, Hao Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2017
- 关键词：biocatalysis cascade reaction 7 beta-hydroxysteroid dehydrogenase multiobjective directed evolution ursodeoxycholic acid
- 质疑
- Efficient production of (R)-o-chloromandelic acid by deracemization of o-chloromandelonitrile with a new nitrilase mined from Labrenzia aggregata被引量：53收藏
- 作者：Zhang, Chen-Sheng Zhang, Zhi-Jun Li, Chun-Xiu Yu, Hui-Lei Zheng, Gao-Wei Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2012
- 关键词：Nitrilase Labrenzia aggregate Enantioselective hydrolysis (R)-o-Chloromandelic acid Biphasic system
- 质疑
- Efficient Reduction of Ethyl 2-Oxo-4-phenylbutyrate at 620 g.L-1 by a Bacterial Reductase with Broad Substrate Spectrum被引量：50收藏
- 作者：Ni, Yan Li, Chun-Xiu Zhang, Jie Shen, Nai-Dong Bornscheuer, Uwe T. Xu, Jian-He
- 机构：E China Univ Sci & Technol;Ernst Moritz Arndt Univ Greifswald
- 来源：ADVANCED SYNTHESIS & CATALYSIS 2011
- 关键词：alcohols asymmetric catalysis cofactor oxidoreductases reduction
- 质疑
- Highly stereoselective reduction of prochiral ketones by a bacterial reductase coupled with cofactor regeneration被引量：46收藏
- 作者：Ni, Yan Li, Chun-Xiu Wang, Li-Juan Zhang, Jie Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：ORGANIC & BIOMOLECULAR CHEMISTRY 2011
- 关键词：Aromatic compounds - Bacteriology - Enantiomers - Ketones
- 质疑
- Enhanced limonene production by optimizing the expression of limonene biosynthesis and MEP pathway genes in E. Coli被引量：38收藏
- 作者：Du, Fu-Liang Yu, Hui-Lei Xu, Jian-He Li, Chun-Xiu
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology
- 来源：Bioresources and Bioprocessing 2014
- 关键词：Biochemistry - Biosynthesis - Flavor compounds - Gene expression - Monoterpenes
- 质疑
- Crosslinking of enzyme coaggregate with polyethyleneimine: A simple and promising method for preparing stable biocatalyst of Serratia marcescens lipase被引量：35收藏
- 作者：Pan, Jiang Kong, Xu-Dong Li, Chun-Xiu Ye, Qin Xu, Jian-He Imanaka, Tadayuki
- 机构：E China Univ Sci & Technol;Ritsumeikan Univ
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2011
- 关键词：Crosslinked enzyme coaggregate Serratia marcescens lipase Polyethyleneimine Coaggregation of enzyme trans-3-(4 '-Methoxyphenyl)glycidic acid methyl ester (MPGM)
- 质疑
- Evolution of Glucose Dehydrogenase for Cofactor Regeneration in Bioredox Processes with Denaturing Agents被引量：35收藏
- 作者：Qian, Wen-Zhuo Ou, Ling Li, Chun-Xiu Pan, Jiang Xu, Jian-He Chen, Qi Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol
- 来源：CHEMBIOCHEM 2020
- 关键词：biocatalysis chemical stability cofactor regeneration directed evolution glucose dehydrogenase
- 质疑
- Enzymatic synthesis of high-titer nicotinamide mononucleotide with a new nicotinamide riboside kinase and an efficient ATP regeneration system被引量：34收藏
- 作者：Qian, Xiao-Long Dai, Yi-Si Li, Chun-Xiu Pan, Jiang Xu, Jian-He Mu, Bozhong
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Suzhou Bioforany EnzyTech Co Ltd
- 来源：BIORESOURCES AND BIOPROCESSING 2022
- 关键词：Enzymatic phosphorylation Nicotinamide riboside Nicotinamide riboside kinase beta-Nicotinamide mononucleotide (NMN) ATP regeneration system
- 质疑
- Highly efficient synthesis of chiral alcohols with a novel NADH-dependent reductase from Streptomyces coelicolor被引量：34收藏
- 作者：Wang, Li-Juan Li, Chun-Xiu Ni, Yan Zhang, Jie Liu, Xiang Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology; School of Chemical and Material Engineering
- 来源：Bioresource Technology 2011
- 关键词：Enzyme activity - Ketones - Escherichia coli
- 质疑
- Identification of a new thermostable and alkali-tolerant α-carbonic anhydrase from Lactobacillus delbrueckii as a biocatalyst for CO2 biomineralization被引量：33收藏
- 作者：Li, Chun-Xiu Jiang, Xiao-Chen Qiu, Yong-Juan Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology; Shanghai Collaborative Innovation Center for Biomanufacturing
- 来源：Bioresources and Bioprocessing 2015
- 关键词：Affinity chromatography - Alkalinity - Biomineralization - Calcite - Calcium carbonate - Dimers - Escherichia coli - Stability
- 质疑
- Continuous Production of Ursodeoxycholic Acid by Using Two Cascade Reactors with Co-immobilized Enzymes被引量：33收藏
- 作者：Zheng, Ming-Min Chen, Fei-Fei Li, Hao Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：CHEMBIOCHEM 2018
- 关键词：domino reactions enzymes immobilization natural products supported catalysts
- 质疑
- Efficient production of (R)-o-chloromandelic acid by deracemization of o-chloromandelonitrile with a new nitrilase mined from Labrenzia aggregata被引量：32收藏
- 作者：Zhang, Chen-Sheng Zhang, Zhi-Jun Li, Chun-Xiu Yu, Hui-Lei Zheng, Gao-Wei Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology
- 来源：Applied Microbiology and Biotechnology 2012
- 关键词：Data mining - Hydrolysis - Lanthanum compounds - Enantioselectivity
- 质疑
- An efficient bioprocess for enzymatic production of L-menthol with high ratio of substrate to catalyst using whole cells of recombinant E. coli被引量：31收藏
- 作者：Zheng, Gao-Wei Pan, Jiang Yu, Hui-Lei Ngo-Thi, Minh-Thu Li, Chun-Xiu Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2010
- 关键词：Whole-cell biocatalysis High ratio of substrate to catalyst High substrate loading Esterase L-Menthol
- 质疑
- De Novo Multienzyme Synthetic Pathways for Lactic Acid Production被引量：31收藏
- 作者：Ding, Xu-Wei Rong, Jing Pan, Ze-Peng Zhu, Xin-Xin Zhu, Zhen-Yu Chen, Qi Zhang, Zhi-Jun Xu, Jian-He Li, Chun-Xiu Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol
- 来源：ACS CATALYSIS 2024
- 关键词：biocatalysis C1 compound lacticacid multienzyme cascade protein engineering
- 质疑
- Immobilization of Bacillus subtilis esterase by simple cross-linking for enzymatic resolution of dl-menthyl acetate被引量：31收藏
- 作者：Zheng, Gao-Wei Yu, Hui-Lei Li, Chun-Xiu Pan, Jiang Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Bioprocessing
- 来源：Journal of Molecular Catalysis B: Enzymatic 2011
- 关键词：Bacteriology - Monoterpenes - Nitrogen compounds - Cloning - Esters - Enzyme immobilization
- 质疑
- Crosslinking of enzyme coaggregate with polyethyleneimine: A simple and promising method for preparing stable biocatalyst of Serratia marcescens lipase被引量：31收藏
- 作者：Pan, Jiang Kong, Xu-Dong Li, Chun-Xiu Ye, Qin Xu, Jian-He Imanaka, Tadayuki
- 机构： State Key Laboratory of Bioreactor Engineering; Department of Biotechnology
- 来源：Journal of Molecular Catalysis B: Enzymatic 2011
- 关键词：Coprecipitation - Enzyme immobilization
- 质疑
- A new thermostable β-glucosidase mined from Dictyoglomus thermophilum: Properties and performance in octyl glucoside synthesis at high temperatures被引量：30收藏
- 作者：Zou, Zheng-Zheng Yu, Hui-Lei Li, Chun-Xiu Zhou, Xin-Wen Hayashi, Chihiro Sun, Jie Liu, Bao-Hong Imanaka, Tadeyuki Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology; Department of Chemistry and Institute of Biomedical Sciences; Department of Biotechnology
- 来源：Bioresource Technology 2012
- 关键词：Cloning - Glucose - Solutions
- 质疑
- Improving Pseudomonas sp. esterase performance by engineering approaches for kinetic resolution of 2-acetoxyphenylacetic acids被引量：29收藏
- 作者：Ju, Xin Pan, Jiang Yu, Hui-Lei Li, Chun-Xiu Xu, Jian-He
- 机构： Lab. of Biocatalysis and Synthetic Biotechnology
- 来源：Biochemical Engineering Journal 2011
- 关键词：Bacteria - Cells - Enzymes - Organic acids - Hydrolysis - Molecular biology - Cell culture - Esters
- 质疑
- A Novel (R)-Imine Reductase from Paenibacillus lactis for Asymmetric Reduction of 3H-Indoles被引量：29收藏
- 作者：Li, Hao Zhang, Guang-Xiang Li, Liu-Mei Ou, Yu-Shi Wang, Ming-Yang Li, Chun-Xiu Zheng, Gao-Wei Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：CHEMCATCHEM 2016
- 关键词：asymmetric reduction biocatalysis imine reductase indoles indolines
- 质疑
- A new thermostable β-glucosidase mined from Dictyoglomus thermophilum: Properties and performance in octyl glucoside synthesis at high temperatures被引量：28收藏
- 作者：Zou, Zheng-Zheng Yu, Hui-Lei Li, Chun-Xiu Zhou, Xin-Wen Hayashi, Chihiro Sun, Jie Liu, Bao-Hong Imanaka, Tadeyuki Xu, Jian-He
- 机构：E China Univ Sci & Technol;Fudan Univ;Fudan Univ;Ritsumeikan Univ
- 来源：BIORESOURCE TECHNOLOGY 2012
- 关键词：beta-Glucosidase Dictyoglomus thermophilum Thermostability Octyl glucoside Reverse hydrolysis
- 质疑
- Enantioselective production of (S)-1-phenyl-1,2-ethanediol from dicarboxyesters by recombinant Bacillus subtilis esterase被引量：27收藏
- 作者：Tian, Xin Zheng, Gao-Wei Li, Chun-Xiu Wang, Zhi-Long Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology; School of Pharmacy
- 来源：Journal of Molecular Catalysis B: Enzymatic 2011
- 关键词：Chlorine compounds - Enantioselectivity - Liquid crystal displays - Enzymes - Esters - Bacteriology - Hydrolysis
- 质疑
