- Immobilization of Serratia marcescens lipase onto amino-functionalized magnetic nanoparticles for repeated use in enzymatic synthesis of Diltiazem intermediate被引量：140收藏
- 作者：Hu, Bin Pan, Jiang Yu, Hui-Lei Liu, Jian-Wen Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：PROCESS BIOCHEMISTRY 2009
- 关键词：Lipase Immobilization Magnetic nanoparticles Enzymatic biocatalytic resolution Diltiazem intermediate Biphasic reaction
- 质疑
- Biochemical properties and potential applications of an organic solvent-tolerant lipase isolated from Serratia marcescens ECU1010被引量：84收藏
- 作者：Zhao, Li-Li Xu, Jian-He Zhao, Jian Pan, Jiang Wang, Zhi-Long
- 机构：E China Univ Sci & Technol;Shanghai Jiao Tong Univ
- 来源：PROCESS BIOCHEMISTRY 2008
- 关键词：lipase Serratia marcescens organic solvent tolerance enantiomeric resolution chiral compounds biochemical properties
- 质疑
- Switching Cofactor Dependence of 7β-Hydroxysteroid Dehydrogenase for Cost-Effective Production of Ursodeoxycholic Acid被引量：66收藏
- 作者：You, Zhi-Neng Chen, Qi Shi, Shou-Cheng Zheng, Ming-Min Pan, Jiang Qian, Xiao-Long Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Suzhou Bioforany EnzyTech Co Ltd
- 来源：ACS CATALYSIS 2019
- 关键词：biocatalysis cofactor dependence small-and-smart library 7 beta-hydroxysteroid dehydrogenase molecular dynamics simulation ursodeoxycholic acid
- 质疑
- Significant enhancement of (R)-mandelic acid production by relieving substrate inhibition of recombinant nitrilase in toluene-water biphasic system被引量：60收藏
- 作者：Zhang, Zhi-Jun Pan, Jiang Liu, Jun-Feng Xu, Jian-He He, Yu-Cai Liu, You-Yan
- 机构：E China Univ Sci & Technol;Changzhou Univ;Guangxi Univ
- 来源：JOURNAL OF BIOTECHNOLOGY 2011
- 关键词：Aqueous-organic biphasic system Recombinant nitrilase Enantioselective hydrolysis High substrate tolerance (R)-(-)-Mandelic acid Escherichia coli whole cells
- 质疑
- Stereoselective synthesis of L-tert-leucine by a newly cloned leucine dehydrogenase from Exiguobacterium sibiricum被引量：58收藏
- 作者：Li, Jing Pan, Jiang Zhang, Jie Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2014
- 关键词：Leucine dehydrogenase Exiguobacterium sibiricum Reductive amination Dual enzyme co-expression L-tert-Leucine
- 质疑
- A thermostable and organic-solvent tolerant esterase from Pseudomonas putida ECU1011: Catalytic properties and performance in kinetic resolution of α-hydroxy acids被引量：49收藏
- 作者：Ma, Bao-Di Yu, Hui-Lei Pan, Jiang Liu, Jia-Yan Ju, Xin Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：BIORESOURCE TECHNOLOGY 2013
- 关键词：Esterase Thermostability Organic-solvent tolerance alpha-Hydroxy (carboxylic) acids Deacetylation
- 质疑
- Rational Engineering of Formate Dehydrogenase Substrate/Cofactor Affinity for Better Performance in NADPH Regeneration被引量：46收藏
- 作者：Jiang, He-Wen Chen, Qi Pan, Jiang Zheng, Gao-Wei Xu, Jian-He
- 机构：East China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2020
- 关键词：Binding affinity Burkholderia stabilis Formate dehydrogenase NADPH regeneration Rational engineering
- 质疑
- An Unusual (R)-Selective Epoxide Hydrolase with High Activity for Facile Preparation of Enantiopure Glycidyl Ethers被引量：45收藏
- 作者：Zhao, Jing Chu, Yan-Yan Li, Ai-Tao Ju, Xin Kong, Xu-Dong Pan, Jiang Tang, Yun Xu, Jian-He
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol
- 来源：ADVANCED SYNTHESIS & CATALYSIS 2011
- 关键词：enantioselectivity epoxide hydrolase glycidyl ethers kinetic resolution molecular modeling
- 质疑
- Biocatalytic synthesis of (R)-(-)-mandelic acid from racemic mandelonitrile by a newly isolated nitrilase-producer Alcaligenes sp ECU0401被引量：44收藏
- 作者：He, Yu Cai Xu, Jian He Xu, Yi Ouyang, Li Ming Pan, Jiang
- 机构：E China Univ Sci & Technol
- 来源：CHINESE CHEMICAL LETTERS 2007
- 关键词：mandelonitrile nitrilase (R)-(-)-mandelic acid screening Alcaligenes sp ECU0401
- 质疑
- Bioproduction of chiral mandelate by enantioselective deacylation of α-acetoxyphenylacetic acid using whole cells of newly isolated Pseudomonas sp ECU1011被引量：38收藏
- 作者：Ju, Xin Yu, Hui-Lei Pan, Jiang Wei, Dong-Zhi Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2010
- 关键词：Microbial esterase Pseudomonas sp. Enantioselective deacylation alpha-Acetoxyphenylacetic acid Enantiopure mandelic acid
- 质疑
- Biochemical properties and potential applications of an organic solvent-tolerant lipase isolated from Serratia marcescens ECU1010被引量：38收藏
- 作者：Zhao, Li-Li Xu, Jian-He Zhao, Jian Pan, Jiang Wang, Zhi-Long
- 机构： Laboratory of Biocatalysis and Bioprocessing; School of Pharmacy
- 来源：Process Biochemistry 2008
- 关键词：Bacteria - Chirality - Dimers - Enantiomers - Enzyme activity - Organic solvents
- 质疑
- Catalytic resolution of (RS)-HMPC acetate by immobilized cells of Acinetobacter sp CGMCC 0789 in a medium with organic cosolvent被引量：37收藏
- 作者：Chen, Y Xu, JH Pan, J Xu, Y Shi, JB
- 机构：E China Univ Chem Technol
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2004
- 关键词：Acinetobacter sp esterase enantioselectivity enzymatic resolution 4-hydroxy-3-methyl-2-(2 '-propenyl)-2-cyclopentenone immobilized cells isopropanol
- 质疑
- Chemoenzymatic deracemization of chiral secondary alcohols:: Process optimization for production of (R)-1-indanol and (R)-1-phenylethanol被引量：36收藏
- 作者：Ou, Ling Xu, Yi Ludwig, Daniel Pan, Jiang Xu, Jian He
- 机构：E China Univ Sci & Technol;Univ Erlangen Nurnberg
- 来源：ORGANIC PROCESS RESEARCH & DEVELOPMENT 2008
- 质疑
- Evolution of Glucose Dehydrogenase for Cofactor Regeneration in Bioredox Processes with Denaturing Agents被引量：35收藏
- 作者：Qian, Wen-Zhuo Ou, Ling Li, Chun-Xiu Pan, Jiang Xu, Jian-He Chen, Qi Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol
- 来源：CHEMBIOCHEM 2020
- 关键词：biocatalysis chemical stability cofactor regeneration directed evolution glucose dehydrogenase
- 质疑
- Crosslinking of enzyme coaggregate with polyethyleneimine: A simple and promising method for preparing stable biocatalyst of Serratia marcescens lipase被引量：35收藏
- 作者：Pan, Jiang Kong, Xu-Dong Li, Chun-Xiu Ye, Qin Xu, Jian-He Imanaka, Tadayuki
- 机构：E China Univ Sci & Technol;Ritsumeikan Univ
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2011
- 关键词：Crosslinked enzyme coaggregate Serratia marcescens lipase Polyethyleneimine Coaggregation of enzyme trans-3-(4 '-Methoxyphenyl)glycidic acid methyl ester (MPGM)
- 质疑
- Immobilization of glycolate oxidase from Medicago falcata on magnetic nanoparticles for application in biosynthesis of glyoxylic acid被引量：34收藏
- 作者：Zhu, Hong Pan, Jiang Hu, Bin Yu, Hui-Lei Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Bioprocessing
- 来源：Journal of Molecular Catalysis B: Enzymatic 2009
- 关键词：Nanomagnetics - Nanoparticles - Fourier transform infrared spectroscopy - Batch reactors - Enzyme immobilization - Enzyme activity - Synthesis (chemical) - Biochemistry - Plants (botany) - Scanning electron microscopy
- 质疑
- Preparation of (R)-(-)-mandelic acid and its derivatives from racemates by enantioselective degradation with a newly isolated bacterial strain Alcaligenes sp ECU0401被引量：34收藏
- 作者：He, Yu-Cai Xu, Jian-He Pan, Jiang Ouyang, Li-Ming Xu, Yi
- 机构：E China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2008
- 关键词：(R)-(-)-mandelic acid Alcaligenes sp ECU0401 (S)-(+)-mandelic acid dehydrogenase enantioselective degradation optical resolution
- 质疑
- Enzymatic synthesis of high-titer nicotinamide mononucleotide with a new nicotinamide riboside kinase and an efficient ATP regeneration system被引量：34收藏
- 作者：Qian, Xiao-Long Dai, Yi-Si Li, Chun-Xiu Pan, Jiang Xu, Jian-He Mu, Bozhong
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Suzhou Bioforany EnzyTech Co Ltd
- 来源：BIORESOURCES AND BIOPROCESSING 2022
- 关键词：Enzymatic phosphorylation Nicotinamide riboside Nicotinamide riboside kinase beta-Nicotinamide mononucleotide (NMN) ATP regeneration system
- 质疑
- Efficient production of diltiazem chiral intermediate using immobilized lipase from serratia marcescens被引量：34收藏
- 作者：Zhao, Li-Li Pan, Jiang Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Bioprocessing
- 来源：Biotechnology and Bioprocess Engineering 2010
- 关键词：Enzyme activity - Ethers - Enzyme immobilization - Tanks (containers)
- 质疑
- Biocatalytic resolution of glycidyl aryl ethers by Trichosporon loubierii:: cell/substrate ratio influences the optical purity of (R)-epoxides被引量：34收藏
- 作者：Xu, Y Xu, JH Pan, J Tang, YF
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2004
- 关键词：cell/substrate ratio enantioselective hydrolysis epoxide hydrolase glycidyl aryl ether Trichosporon loubierii
- 质疑
- Significant enhancement of (R)-mandelic acid production by relieving substrate inhibition of recombinant nitrilase in toluene-water biphasic system被引量：33收藏
- 作者：Zhang, Zhi-Jun Pan, Jiang Liu, Jun-Feng Xu, Jian-He He, Yu-Cai Liu, You-Yan
- 机构： Laboratory of Biocatalysis and Bioprocessing; College of Chemistry and Chemical Engineering; College of Chemistry and Chemical Engineering
- 来源：Journal of Biotechnology 2011
- 关键词：Cells - Cytology - Enantioselectivity - Escherichia coli - Hydrolysis - Organic acids - Organic solvents - Productivity - Toluene
- 质疑
- Resolution of racemic sulfoxides with high productivity and enantioselectivity by a Rhodococcus sp. strain as an alternative to biooxidation of prochiral sulfides for efficient production of enantiopure sulfoxides被引量：32收藏
- 作者：Li, Ai-Tao Yu, Hui-Lei Pan, Jiang Zhang, Jian-Dong Xu, Jian-He Lin, Guo-Qiang
- 机构： Laboratory of Biocatalysis and Bioprocessing; Shanghai Institute of Organic Chemistry
- 来源：Bioresource Technology 2011
- 关键词：Substrates - Enantioselectivity
- 质疑
- Efficient production of l-menthol in a two-phase system with SDS using an immobilized bacillus subtilis esterase被引量：32收藏
- 作者：Pan, Jiang Dang, Ngoc-Duy Zheng, Gao-Wei Cheng, Bo Ye, Qin Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology; Sunflower Pharmaceutical Group Company Limited
- 来源：Bioresources and Bioprocessing 2014
- 关键词：Cloning - Crosslinking - Enantioselectivity - Enzymatic hydrolysis - Enzyme activity - Enzyme inhibition - Esters - Sulfur compounds
- 质疑
- An efficient bioprocess for enzymatic production of L-menthol with high ratio of substrate to catalyst using whole cells of recombinant E. coli被引量：31收藏
- 作者：Zheng, Gao-Wei Pan, Jiang Yu, Hui-Lei Ngo-Thi, Minh-Thu Li, Chun-Xiu Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2010
- 关键词：Whole-cell biocatalysis High ratio of substrate to catalyst High substrate loading Esterase L-Menthol
- 质疑
- Isolation and properties of a levo-lactonase from Fusarium proliferatum ECU2002: A robust biocatalyst for production of chiral lactones被引量：31收藏
- 作者：Zhang, Xian Xu, Jian-He Xu, Yi Pan, Jiang
- 机构： Laboratory of Biocatalysis and Bioprocessing
- 来源：Applied Microbiology and Biotechnology 2007
- 关键词：Aldehydes - Bacteria - Concentration (process) - Electrophoresis - Ketones
- 质疑
- Crosslinking of enzyme coaggregate with polyethyleneimine: A simple and promising method for preparing stable biocatalyst of Serratia marcescens lipase被引量：31收藏
- 作者：Pan, Jiang Kong, Xu-Dong Li, Chun-Xiu Ye, Qin Xu, Jian-He Imanaka, Tadayuki
- 机构： State Key Laboratory of Bioreactor Engineering; Department of Biotechnology
- 来源：Journal of Molecular Catalysis B: Enzymatic 2011
- 关键词：Coprecipitation - Enzyme immobilization
- 质疑
- Immobilization of Bacillus subtilis esterase by simple cross-linking for enzymatic resolution of dl-menthyl acetate被引量：31收藏
- 作者：Zheng, Gao-Wei Yu, Hui-Lei Li, Chun-Xiu Pan, Jiang Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Bioprocessing
- 来源：Journal of Molecular Catalysis B: Enzymatic 2011
- 关键词：Bacteriology - Monoterpenes - Nitrogen compounds - Cloning - Esters - Enzyme immobilization
- 质疑
- Significantly improved asymmetric oxidation of sulfide with resting cells of Rhodococcus sp. in a biphasic system被引量：30收藏
- 作者：Li, Ai-Tao Zhang, Jian-Dong Yu, Hui-Lei Pan, Jiang Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Bioprocessing
- 来源：Process Biochemistry 2011
- 关键词：Oxidation - Enantiomers - Paraffins - Alcohols - Polyols - Solvent extraction
- 质疑
- Efficient synthesis of a statin precursor in high space-time yield by a new aldehyde-tolerant aldolase identified from Lactobacillus brevis被引量：30收藏
- 作者：Jiao, Xue-Cheng Pan, Jiang Xu, Guo-Chao Kong, Xu-Dong Chen, Qi Zhang, Zhi-Jun Xu, Jian-He
- 机构：E China Univ Sci & Technol; E China Univ Sci & Technol
- 来源：CATALYSIS SCIENCE & TECHNOLOGY 2015
- 关键词：Crystal structure - Stereochemistry - Acetaldehyde - Hydrogen bonds - Ketones - Stability - Substrates
- 质疑
- Highly Efficient Synthesis of (R)-3-Quinuclidinol in a Space-Time Yield of 916 g L-1 d-1 Using a New Bacterial Reductase ArQR被引量：30收藏
- 作者：Zhang, Wen-Xia Xu, Guo-Chao Huang, Lei Pan, Jiang Yu, Hui-Lei Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：ORGANIC LETTERS 2013
- 质疑
