- Nitrogen metabolism of asparagine and glutamate in Vero cells studied by 1H/15N NMR spectroscopy被引量：52收藏
- 作者：Huang, Haiyan Yu, Yihua Yi, Xiaoping Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering; Shanghai Key Laboratory of Functional Magnetic Resonance Imaging
- 来源：Applied Microbiology and Biotechnology 2007
- 关键词：Ammonia - Metabolism - Metabolites - Nitrogen - Nuclear magnetic resonance spectroscopy
- 质疑
- Low-dose effects on thyroid disruption in zebrafish by long-term exposure to oxytetracycline被引量：50收藏
- 作者：Yu, Kan Li, Xiaoyong Qiu, Yushu Zeng, Xinxin Yu, Xiaogang Wang, Weiye Yi, Xiaoping Huang, Lisu
- 机构：East China Univ Sci & Technol;Shanghai Jiao Tong Univ;Shanghai Jiao Tong Univ;Shanghai Jiao Tong Univ
- 来源：AQUATIC TOXICOLOGY 2020
- 关键词：Antibiotic exposure Developmental toxicity Thyroid disruption Oxytetracycline in the aquatic environment
- 质疑
- Regulation of porcine circovirus type 2-like particles expressed in baculovirus expression system被引量：35收藏
- 作者：Li, Yingjun Yi, Xiaoping Zhuang, Yingping Chu, Ju
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresources and Bioprocessing 2016
- 关键词：Animals - Transcription - Viruses
- 质疑
- Metabolic characteristics of recombinant Chinese hamster ovary cells expressing glutamine synthetase in presence and absence of glutamine被引量：32收藏
- 作者：Zhang, Fang Sun, Xiangming Yi, Xiaoping Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：CYTOTECHNOLOGY 2006
- 关键词：animal cell culture cell metabolism glutamine glutamine synthetase recombinant CHO cell
- 质疑
- Novel scale-up strategy based on three-dimensional shear space for animal cell culture被引量：22收藏
- 作者：Li, Chao Teng, Xiaonuo Peng, Huadong Yi, Xiaoping Zhuang, Yingping Zhang, Siliang Xia, Jianye
- 机构：East China Univ Sci & Technol;Imperial Coll London;Imperial Coll London
- 来源：CHEMICAL ENGINEERING SCIENCE 2020
- 关键词：Shear rate Animal cell culture Scale-up Computational fluid dynamics Stirred tank bioreactor
- 质疑
- Application of semi-supervised convolutional neural network regression model based on data augmentation and process spectral labeling in Raman predictive modeling of cell culture processes被引量：22收藏
- 作者：Min, Rui Wang, Zhi Zhuang, Yingping Yi, Xiaoping
- 机构：East China Univ Sci & Technol
- 来源：BIOCHEMICAL ENGINEERING JOURNAL 2023
- 关键词：Semi -supervised learning Convolutional neural network regression Data augmentation Process spectra labeling
- 质疑
- Gene transcription acceleration: Main cause of hepatitis B surface antigen production improvement by dimethyl sulfoxide in the culture of Chinese hamster ovary cells被引量：19收藏
- 作者：Wang, Wenying Yi, Xiaoping Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2007
- 关键词：cell culture CHO cell dimethyl sulfoxide (DMSO) hepatitis B surface antigen gene transcription
- 质疑
- 生物反应器及其研究技术进展被引量：16收藏
- 作者：王永红 夏建业 唐寅 杭海峰 易小萍 潘江 许建和 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《生物加工过程》 2013
- 关键词：生物反应器 计算流体力学 微型生物反应器 动物细胞反应器 酶反应器
- 摘要：阐述了生物反应器设计、放大的新理念及关键技术发展,并在此基础上综述了应用于生物技术产品生产的生物反应器的主要发展趋势,包括以代谢流分析为核心的生物反应器系统、基于计算流体力学模拟技术的传统发酵罐改良、微型生物反应器、动物...
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- Long-term low-dose oxytetracycline potentially leads to neurobehavioural changes被引量：14收藏
- 作者：Qiu, Yushu Yu, Kan Yu, Xiaogang Yi, Xiaoping Cai, Kang Li, Huajun Xu, Shanshan Wang, Weiye Huang, Lisu
- 机构：Shanghai Jiao Tong Univ;East China Univ Sci & Technol;Shanghai Jiao Tong Univ;Shanghai Jiao Tong Univ;Fudan Univ
- 来源：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 2021
- 关键词：Antibiotic exposure Oxytetracycline in the environment Behaviour change Neurotransmitter Thyroid disruption
- 质疑
- Improvement of Vero cell growth in glutamate-based culture by supplementing ammoniagenic compounds被引量：13收藏
- 作者：Huang, Haiyan Yi, Xiaoping Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：PROCESS BIOCHEMISTRY 2006
- 关键词：alanine asparagine glutamate glutamine-free glutamine synthetase Vero cell
- 质疑
- Hydrodynamic investigation of a novel shear-generating device for the measurement of anchorage-dependent cell adhesion intensity被引量：11收藏
- 作者：Wang, Han Xia, Jianye Zheng, Zhiming Zhuang, Ying-Ping Yi, Xiaoping Zhang, Dahe Wang, Peng
- 机构：Chinese Acad Sci;Univ Sci & Technol China;East China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2018
- 关键词：Animal cell culture Adhesion force Energy dissipation rate Microcarriers Modeling
- 质疑
- Tumor microenvironment responsive multifunctional smart living materials based on engineered bacteria for inducing macrophage polarization to enhance tumor immunotherapy被引量：11收藏
- 作者：Jiang, Ning Zhao, Kui Liu, Chengcheng Zhu, Xiaojuan Huang, Xumeng Yang, Lin Yi, Xiaoping Zhuang, Yingping Ye, Bangce Qian, Jiangchao Huang, Jiaofang
- 机构：East China Univ Sci & Technol ECUST;East China Univ Sci & Technol;Xian Jiao Tong Univ Hlth Sci Ctr;Jiangxi Normal Univ JXNU;East China Univ Sci & Technol
- 来源：CHEMICAL ENGINEERING JOURNAL 2024
- 关键词：Biomaterials Biotechnology Engineered living material Targeted therapy Drug delivery
- 质疑
- Surface modification of non-woven poly (ethylene terephthalate) fibrous scaffold for improving cell attachment in animal cell culture被引量：10收藏
- 作者：Zhang, Weiyuan Yi, Xiaoping Sun, Xiangming Zhang, Yuanxing
- 机构：E China Univ Chem Technol
- 来源：JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 2008
- 关键词：chemical modification poly (ethylene terephthalate) hydrophilicity fabric scaffold cell attachment animal cell culture
- 质疑
- Enhanced intracellular accumulation of recombinant HBsAg in CHO cells by dimethyl sulfoxide被引量：10收藏
- 作者：Ma, Zheyong Yi, Xiaoping Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：PROCESS BIOCHEMISTRY 2008
- 关键词：cell culture recombinant CHO cell dimethyl sulfoxide HBsAg sucrose gradient analysis intracellular accumulation
- 质疑
- Large-scale plasmid preparation for transient gene expression被引量：10收藏
- 作者：Cheng, Lu Sun, Xiangming Yi, Xiaoping Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Shanghai Kexin Biotech Co Ltd
- 来源：BIOTECHNOLOGY LETTERS 2011
- 关键词：CHO cells Fed-batch Plasmid DNA RNase Transient transfection Ultrafiltration
- 质疑
- Coupling metabolomics analysis and DOE optimization strategy towards enhanced IBDV production by chicken embryo fibroblast DF-1 cells被引量：10收藏
- 作者：Lin, Jia Yi, Xiaoping Zhuang, Yingping
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2020
- 关键词：DF-1 IBDV Metabolome DOE methodology
- 质疑
- A novel scale-up strategy for cultivation of BHK-21 cells based on similar hydrodynamic environments in the bioreactors被引量：9收藏
- 作者：Teng, Xiaonuo Li, Chao Yi, Xiaoping Zhuang, Yingping
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2021
- 关键词：BHK-21 cells Bioreactor Computational fluid dynamics Hydrodynamic characteristics Scale-up
- 质疑
- Limitations in the process of transcription and translation inhibit recombinant human chorionic gonadotropin expression in CHO cells被引量：8收藏
- 作者：Liu, Yang Yi, Xiaoping Zhuang, Yingping Zhang, Siliang
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2015
- 关键词：Transcription limitation Recombinant protein Mammalian cell culture
- 质疑
- MDCK细胞微载体悬浮培养放大工艺研究被引量：8收藏
- 作者：龚迪 易小萍 张元兴
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《中国生物工程杂志》 2012
- 关键词：MDCK细胞 微载体 低血清培养基 消化放大
- 摘要：MDCK细胞对各种流感病毒具有高度敏感性,广泛应用于流感病毒的分离和疫苗制备。通过探索培养基中促进细胞贴壁的关键组分,并筛选水解物,开发了适合MDCK细胞生长的低血清培养基。发现钙、镁离子是细胞贴壁不可缺少的物质,麦麸水...
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- 适于重组CHO细胞培养的无血清培养基的制备被引量：8收藏
- 作者：张大鹤 易小萍 张元兴 孙祥明
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《中国生物制品学杂志》 2011
- 关键词：CHO细胞 培养基,无血清
- 摘要：目的制备适于重组CHO细胞培养的无血清培养基(Serum-free medium,SFM)。方法在本室制备的无血清、低蛋白培养基SFMC的基础上,开发支持重组CHO-K1细胞生长的无动物源、无蛋白培养基(Protein-...
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- Development of a simple and high-yielding fed-batch process for the production of porcine circovirus type 2 virus-like particle subunit vaccine被引量：8收藏
- 作者：Cao, Wenlong Cao, Hui Yi, Xiaoping Zhuang, Yingping
- 机构：East China Univ Sci & Technol;Zhejiang EBVAC Biotech Co Ltd
- 来源：AMB EXPRESS 2019
- 关键词：Virus-like particles Porcine circovirus Subunit vaccine Fed-batch process Baculoviral expression system Feeding "cocktail"
- 质疑
- PK-15细胞微载体悬浮培养生产猪细小病毒的工艺研究被引量：7收藏
- 作者：李智力 易小萍 储炬 庄英萍 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《中国生物工程杂志》 2015
- 关键词：PK-15细胞 PPV病毒 微载体 反应器
- 摘要：通过对猪细小病毒接毒时间TOI、MOI和收毒时间进行优化,开发了一种基于PK-15细胞静置培养的猪细小病毒生产工艺,最大病毒滴度达到107.5TCID50/ml。通过进一步优化接毒时间,成功建立了基于PK-15细胞反应器...
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- Functional Living Materials for Virus Disinfection Based on Engineered Bacillus subtilis Biofilm by Displaying Viral Affinity Peptides and Expressing Protease被引量：7收藏
- 作者：Zhu, Xiaojuan Chen, Jianshu Luo, Jian Liu, Xue Xu, Yan Chen, Lin Dong, Hao Zhang, Lingfan Yang, Lin Jiang, Nin Huang, Xumeng Mohsin, Ali Yi, Xiaoping Wang, Yonghong Zhuang, Yingping Zhang, Xuehong Zhang, Ke Zhang, Lixin Zhong, Chao Huang, Jiaofang
- 机构：East China Univ Sci & Technol ECUST;Shanghai Inst Biol Prod Co Ltd;Chinese Acad Sci;Ocean Univ China;East China Univ Sci & Technol
- 来源：ADVANCED FUNCTIONAL MATERIALS 2024
- 关键词：Bacillus subtilis biofilm engineered living materials SARS-CoV-2 virus disinfection
- 质疑
- A study on enhanced O-glycosylation strategy for improved production of recombinant human chorionic gonadotropin in Chinese hamster ovary cells被引量：5收藏
- 作者：Deng, Zhe Yi, Xiaoping Chu, Ju Zhuang, Yingping
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2019
- 关键词：Recombinant human chorionic gonadotropin CHO cell O-glycosylation GalNAc Ugp2 Galnt1
- 质疑
- 瞬时基因表达可溶性的VEGFR2：I-IV被引量：5收藏
- 作者：李军 易小萍 张元兴 孙祥明
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《生物工程学报》 2008
- 关键词：VEGFR2 瞬时基因表达 HEK293 PEI
- 摘要：通过RT-PCR的方法从三个月的流产绒毛组织中克隆目的基因VEGFR2(Vascular endothelial growth factor receptor 2,血管内皮细胞生长因子受体2)胞外I-IV区,连接到真核表...
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- Medium optimization based on comparative metabolomic analysis of chicken embryo fibroblast DF-1 cells被引量：5收藏
- 作者：Lin, Jia Yi, Xiaoping Zhuang, Yingping
- 机构：East China Univ Sci & Technol
- 来源：RSC ADVANCES 2019
- 关键词：Animals - Cell culture - Metabolites - Viruses - Cell proliferation - Disease control
- 质疑
- PK-15细胞微载体悬浮培养及PCV2增殖工艺研究被引量：5收藏
- 作者：梁武 乔健 李亚杰 易小萍 杨保收
- 机构：中国农业大学动物医学院;天津瑞普生物技术股份有限公司;华东理工大学生物反应器国家重点实验室
- 来源：《中国兽医杂志》 2019
- 关键词：PK15细胞 猪圆环病毒2型 微载体 悬浮培养
- 摘要：研究表明,PCV2仅在PK15等少数哺乳动物细胞上增殖,但由于PCV2毒力弱,且不产生细胞病变,获得高滴度病毒难度较大[1]。因此,PCV2的培养滴度高低已成为制约现有疫苗质量的关键瓶颈之一。为建立在生物反应器内微载体逐...
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- 重组CHO-GS细胞降低氨毒副作用的代谢研究被引量：5收藏
- 作者：张芳 易小萍 孙祥明 张元兴
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《生物工程学报》 2006
- 关键词：重组CHO细胞 谷氨酰胺合成酶 无血清培养 代谢 氨
- 摘要：在重组CHOGS细胞无血清批培养过程中,由于GS系统的引入,使氨对细胞的毒副作用显著降低,从而引起细胞生长和代谢途径发生变化。当起始氨浓度为1.42mmolL时,细胞最高密度可达到15.6×105cellsmL,随着氨浓...
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- 温度对重组BHK细胞生长、代谢和vWF蛋白表达的影响被引量：4收藏
- 作者：易小萍 孙祥明 张元兴
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2007
- 关键词：重组BHK细胞 细胞培养 温度 细胞周期 细胞凋亡 vWF
- 摘要：通过重组幼仓鼠肾细胞(BHK细胞)在不同温度下的批培养和脉冲培养,研究了温度对重组BHK细胞生长、代谢以及血管性血友病因子(vWF)蛋白表达的影响。相对于37℃,升高和降低温度的培养都降低了细胞的生长速率和密度,降温的作...
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- Enhancement of hepatitis B surface antigen production by adenosine 5′-monophosphate in culture of recombinant Chinese hamster ovary cells被引量：4收藏
- 作者：Luo, Weiwen Sun, Xiangming Yi, Xiaoping Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOSCIENCE AND BIOENGINEERING 2005
- 关键词：animal cell culture adenosine 5 '-monophosphate adenosine 5 '-triphosphate Chinese hamster ovary (CHO) cell hepatitis B surface antigen (HBsAg)
- 质疑
