杭海峰 收藏
( 任职于 生物工程学院)
生物工程专业：硕士生导师、微生物学专业：硕士生导师、生物工程领域：工程硕士导师 研究方向：生物过程检测与控制；生物过程优化与放大；微生物代谢调控 工作经历： 1998.7至今华东理工大学生物工程学院 国家生化工程技术研究中心（上海） 2014.1-2015.1美国Washington University in St. Louis，访问学者 副研究员，工学博士。 1998年硕士毕业于华东理工大学生物化工专业并留校工作，2008年获得博士学位。2014-2015年获得国家留学基金委资助，在美国圣路易斯华盛顿大学做访问学者。主要从事生物过程检测与控制系统... 详细>>
- Metabolic engineering coupled with adaptive evolution strategies for the efficient production of high-quality L-lactic acid by Lactobacillus paracasei被引量：65收藏
- 作者：Tian, Xiwei Liu, Xuehua Zhang, Yifan Chen, Yang Hang, Haifeng Chu, Ju Zhuang, Yingping
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCE TECHNOLOGY 2021
- 关键词：Lactobacillus paracasei Lactic acid CRISPR Cas9 adaptive evolution Open fermentation
- 质疑
- Oxygen uptake rate optimization with nitrogen regulation for erythromycin production and scale-up from 50 L to 372 m3 scale被引量：64收藏
- 作者：Zou, Xiang Hang, Hai-feng Chu, Ju Zhuang, Ying-ping Zhang, Si-liang
- 机构：E China Univ Sci & Technol;Southwest Univ
- 来源：BIORESOURCE TECHNOLOGY 2009
- 关键词：Erythromycin Industrial scale Metabolic flux Oxygen uptake rate Scale-up
- 质疑
- Xanthan-Curdlan nexus for synthesizing edible food packaging films被引量：61收藏
- 作者：Mohsin, Ali Zaman, Waqas Qamar Guo, Meijin Ahmed, Waheed Khan, Imran Mahmood Niazi, Sobia Rehman, Abdur Hang, Haifeng Zhuang, Yingping
- 机构：East China Univ Sci & Technol;Natl Univ Sci & Technol NUST;Jiangnan Univ
- 来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2020
- 关键词：Xanthangum Curdlan Blendfilms
- 质疑
- Sustainable biosynthesis of curdlan from orange waste by using Alcaligenes faecalis: A systematically modeled approach被引量：48收藏
- 作者：Mohsin, Ali Sun, Jingyun Khan, Imran Mahmood Hang, Haifeng Tariq, Muhammad Tian, Xiwei Ahmed, Waqas Niazi, Sobia Zhuang, Yingping Chu, Ju Mohsin, Muhammad Zubair Salim-ur-Rehman Guo, Meijin
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Jiangnan Univ;Univ Agr Faisalabad;Univ Agr Faisalabad
- 来源：CARBOHYDRATE POLYMERS 2019
- 关键词：Sustainable biosynthesis Orange peels Kinetics Curdlan Batch culture fermentation
- 质疑
- Effect of glycosylation on biochemical characterization of recombinant phytase expressed in Pichia pastoris被引量：44收藏
- 作者：Guo, Meijin Hang, Haifeng Zhu, Taicheng Zhuang, Yingping Chu, Ju Zhang, Siliang
- 机构：E China Univ Sci & Technol;Jiangxi Agr Univ
- 来源：ENZYME AND MICROBIAL TECHNOLOGY 2008
- 关键词：phytase glycosylation biochemical characterization thermostability micro-heterogeneity
- 质疑
- Kinetic analysis of curdlan production by Alcaligenes faecalis with maltose, sucrose, glucose and fructose as carbon sources被引量：42收藏
- 作者：Zhang, Qin Sun, Jingyun Wang, Zejian Hang, Haifeng Zhao, Wei Zhuang, Yingping Chu, Ju
- 机构： State Key Laboratory of Bioreactor Engineering; Shan Dong Fu Yang Biological Technology Co.
- 来源：Bioresource Technology 2018
- 关键词：Carbon - Rate constants - Fructose - Sugar (sucrose) - Biochemistry - Glucose - Growth kinetics - Fermentation - Maltose - Cell growth
- 质疑
- Enhancement of erythromycin A production with feeding available nitrogen sources in erythromycin biosynthesis phase被引量：36收藏
- 作者：Zou, Xiang Hang, Hai-feng Chu, Ju Zhuang, Ying-ping Zhang, Si-liang
- 机构：E China Univ Sci & Technol;Southwest Univ
- 来源：BIORESOURCE TECHNOLOGY 2009
- 关键词：Available nitrogen sources Biosynthesis phase Erythromycin A Industrial scale Scale up
- 质疑
- 人工神经网络在红霉素发酵过程状态预估中的应用被引量：34收藏
- 作者：黄明志 杭海峰 储炬 叶勤 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2000
- 关键词：神经网络 BP网络 RBF网络 红霉素 发酵 状态预估
- 摘要：探索了动态 BP网络和 RBF网络在红霉素发酵过程状态预估中的应用 ,比较了它们的收敛速度和学习能力。结果表明 ,BP网络和 RBF网络都具有相当好的学习能力 ,但 RBF网络的收敛速度更快。训练好的神经网络 ,在红霉素...
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- Kinetic analysis of curdlan production by Alcaligenes faecalis with maltose, sucrose, glucose and fructose as carbon sources被引量：33收藏
- 作者：Zhang, Qin Sun, Jingyun Wang, Zejian Hang, Haifeng Zhao, Wei Zhuang, Yingping Chu, Ju
- 机构：East China Univ Sci & Technol;Shan Dong Fu Yang Biol Technol Co Ltd
- 来源：BIORESOURCE TECHNOLOGY 2018
- 关键词：Alcaligenes faecalis Carbon sources Curdlan Kinetics
- 质疑
- Rationally optimized generation of integrated Escherichia coli with stable and high yield lycopene biosynthesis from heterologous mevalonate (MVA) and lycopene expression pathways被引量：32收藏
- 作者：Hussain, Muhammad Hammad Hong, Qi Zaman, Waqas Qamar Mohsin, Ali Wei, Yanlong Zhang, Ning Fang, Hongqing Wang, Zejian Hang, Haifeng Zhuang, Yingping Guo, Meijin
- 机构：East China Univ Sci & Technol;Natl Univ Sci & Technol NUST;Beijing Inst Biotechnol
- 来源：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY 2021
- 关键词：Escherichia coli Lycopene biosynthesis Rational model of expression pathways Integrated MVA pathway Fermentation
- 质疑
- 基因工程菌Pichia pastoris高密度培养条件研究被引量：29收藏
- 作者：郭美锦 吴康华 杭海峰 储炬 庄英萍 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《微生物学通报》 2001
- 关键词：基因工程菌 毕赤酵母 高密度培养
- 摘要：基因工程菌pichiapastoris最佳种子培养基为添加 4mL/LPTMl的BMGY培养基 ;全合成高密度摇瓶培养基是甘油 4% ,(NH4) 2 SO41 0g/L ,CaSO40 93g/L ,K2 SO41 ...
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- Mixomics analysis of Bacillus subtilis: effect of oxygen availability on riboflavin production被引量：28收藏
- 作者：Hu, Junlang Lei, Pan Mohsin, Ali Liu, Xiaoyun Huang, Mingzhi Li, Liang Hu, Jianhua Hang, Haifeng Zhuang, Yingping Guo, Meijin
- 机构：East China Univ Sci & Technol;Shanghai Acebright Pharmaceut Grp Co Ltd
- 来源：MICROBIAL CELL FACTORIES 2017
- 关键词：Riboflavin Bacillus subtilis Oxygen availability C-13 metabolic flux analysis Metabolomics Transcriptomics
- 质疑
- Effect of glycosylation on biochemical characterization of recombinant phytase expressed in Pichia pastoris被引量：25收藏
- 作者：Guo, Meijin Hang, Haifeng Zhu, Taicheng Zhuang, Yingping Chu, Ju Zhang, Siliang
- 机构： State Key Laboratory of Bioreactor Engineering; Department of Biotechnology
- 来源：Enzyme and Microbial Technology 2008
- 关键词：Amino acids - Biochemical engineering - Glycosylation - pH - Thermodynamic stability
- 质疑
- 培养条件对重组毕赤酵母高密度表达猪胰岛素前体的影响被引量：23收藏
- 作者：姚钰舜 储炬 杭海峰 庄英萍 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2006
- 关键词：毕赤酵母 猪胰岛素前体 高密度培养 高表达
- 摘要：研究了基因工程菌P ich ia p astotis高密度、高表达的培养条件。在全合成摇瓶培养基的基础上,考察了诱导过程中甲醇浓度、温度、pH、(NH4)2SO4及磷酸盐浓度等因素对猪胰岛素前体(P IP)表达的影响。研...
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- Transcriptional investigation of the effect of mixed feeding to identify the main cellular stresses on recombinant Pichia pastoris被引量：21收藏
- 作者：Zhu, Taicheng Hang, Haifeng Chu, Ju Zhuang, Yingping Zhang, Siliang Guo, Meijin
- 机构： Institute of Microbiology; State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Industrial Microbiology and Biotechnology 2013
- 关键词：Genes - Insulin - Metabolism - Methanol - Physiology - Recombinant proteins - Yeast
- 质疑
- Efficient generation of male germ-like cells derived during co-culturing of adipose-derived mesenchymal stem cells with Sertoli cells under retinoic acid and testosterone induction被引量：21收藏
- 作者：Luo, Yanxia Xie, Lili Mohsin, Ali Ahmed, Waqas Xu, Chenze Peng, Yan Hang, Haifeng Zhuang, Yingping Chu, Ju Guo, Meijin
- 机构：East China Univ Sci & Technol
- 来源：STEM CELL RESEARCH & THERAPY 2019
- 关键词：adipose-derived mesenchymal stem cells Germ cell Sertoli cell Signaling pathways Retinoic acid Testosterone Synergistic effect
- 质疑
- A simple fermentation strategy for high-level production of recombinant phytase by Pichia pastoris using glucose as the growth substrate被引量：18收藏
- 作者：Hang, Haifeng Ye, Xinhao Guo, Meijin Chu, Ju Zhuang, Yingping Zhang, Ming Zhang, Siliang
- 机构： State Key Laboratory of Bioreactor Engineering; Department of Biotechnology
- 来源：Enzyme and Microbial Technology 2009
- 关键词：Substrates - Yeast - Fermentation - Methanol - Recombinant proteins - Glucose oxidase - Glucose sensors
- 质疑
- Kinetic analysis of sodium gluconate production by Aspergillus niger with different inlet oxygen concentrations被引量：18收藏
- 作者：Tian, Xiwei Shen, Yuting Zhuang, Yingping Zhao, Wei Hang, Haifeng Chu, Ju
- 机构：East China Univ Sci & Technol;Shan Dong Fuyang Biol Technol Co Ltd
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2018
- 关键词：Aspergillus niger Sodium gluconate Oxygen Kinetics
- 质疑
- Application of oxygen uptake rate and response surface methodology for erythromycin production by Saccharopolyspora erythraea被引量：18收藏
- 作者：Zou, Xiang Hang, Hai-feng Chen, Chang-fa Chu, Ju Zhuang, Ying-ping Zhang, Si-liang
- 机构：E China Univ Sci & Technol;Southwest Univ
- 来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2008
- 关键词：Bioreactors Erythromycin Fed-batch culture Physiology Process integration Response surface methodology
- 质疑
- Target-site directed rational high-throughput screening system for high sophorolipids production by Candida bombicola被引量：17收藏
- 作者：Chen, Yang Tian, Xiwei Li, Qianhui Li, Ya Chu, Ju Hang, Haifeng Zhuang, Yingping
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCE TECHNOLOGY 2020
- 关键词：Candida bombicola Sophorolipids Combined mutagenesis Relaxation culture Combined pressure
- 质疑
- Exploring the metabolic fate of propanol in industrial erythromycin-producing strain via 13C labeling experiments and enhancement of erythromycin production by rational metabolic engineering of Saccharopolyspora erythraea被引量：16收藏
- 作者：Xu, Feng Ke, Xiang Hong, Ming Huang, Mingzhi Chen, Chongchong Tian, Xiwei Hang, Haifeng Chu, Ju
- 机构：East China Univ Sci & Technol
- 来源：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2021
- 关键词：Saccharopolyspora erythraea Erythromycin Metabolic flux analysis N-Propanol Succinyl-CoA synthetase
- 质疑
- A simple fermentation strategy for high-level production of recombinant phytase by Pichia pastoris using glucose as the growth substrate被引量：16收藏
- 作者：Hang, Haifeng Ye, Xinhao Guo, Meijin Chu, Ju Zhuang, Yingping Zhang, Ming Zhang, Siliang
- 机构：E China Univ Sci & Technol;Jiangxi Agr Univ
- 来源：ENZYME AND MICROBIAL TECHNOLOGY 2009
- 关键词：Pichia pastoris Phytase Glucose Feeding strategy
- 质疑
- 生物反应器及其研究技术进展被引量：16收藏
- 作者：王永红 夏建业 唐寅 杭海峰 易小萍 潘江 许建和 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《生物加工过程》 2013
- 关键词：生物反应器 计算流体力学 微型生物反应器 动物细胞反应器 酶反应器
- 摘要：阐述了生物反应器设计、放大的新理念及关键技术发展,并在此基础上综述了应用于生物技术产品生产的生物反应器的主要发展趋势,包括以代谢流分析为核心的生物反应器系统、基于计算流体力学模拟技术的传统发酵罐改良、微型生物反应器、动物...
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- A simple unstructured model-based control for efficient expression of recombinant porcine insulin precursor by Pichia pastoris被引量：16收藏
- 作者：Hang, Hai-feng Chen, Wen Guo, Mei-jin Chu, Ju Zhuang, Ying-ping Zhang, Siliang
- 机构：E China Univ Sci & Technol;Jiangxi Agr Univ
- 来源：KOREAN JOURNAL OF CHEMICAL ENGINEERING 2008
- 关键词：Pichia pastoris Porcine Insulin Precursor Monod Modeling Methanol Feeding Strategy
- 质疑
- 重组人血清白蛋白表达研究进展被引量：15收藏
- 作者：郭美锦 储炬 杭海峰 庄英萍 叶勤 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《生物工程进展》 2000
- 关键词：人血清 白蛋白 表达系统 基因工程 基因重组
- 摘要：本文综述了重组人血清白蛋白在细菌 ,酵母 ,植物和动物等表达系统中表达的研究进展。用酵母表达系统 ,尤其是毕赤酵母 ,表达的重组白蛋白产量高且提取工艺简单 ,是其产业化最具有前途的表达系统。同时在转基因动物、植物中培养表...
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- Application of oxygen uptake rate and response surface methodology for erythromycin production by Saccharopolyspora erythraea被引量：15收藏
- 作者：Zou, Xiang Hang, Hai-Feng Chen, Chang-Fa Chu, Ju Zhuang, Ying-Ping Zhang, Si-Liang
- 机构： State Key Laboratory of Bioreactor Engineering; School of Pharmaceutical Sciences
- 来源：Journal of Industrial Microbiology and Biotechnology 2008
- 关键词：Amino acids - Antibiotics - Batch cell culture - Biochemistry - Bioreactors - Citric acid - Cultivation - Nutrients - Oxygen - Physiological models - Sulfur compounds - Surface properties
- 质疑
- Oxygen uptake rate optimization with nitrogen regulation for erythromycin production and scale-up from 50 L to 372 m3 scale被引量：14收藏
- 作者：Zou, Xiang Hang, Hai-feng Chu, Ju Zhuang, Ying-ping Zhang, Si-liang
- 机构： State Key Laboratory of Bioreactor Engineering; School of Pharmaceutical Sciences
- 来源：Bioresource Technology 2009
- 关键词：Biochemistry - Metabolism - Biosynthesis - Fermenters - Lactic acid - Enzyme activity - Fermentation - Nitrogen - Antibiotics - Oxygen - Thermodynamic stability - Physiology - Propionic acid
- 质疑
- Differentiation roadmap of embryonic Sertoli cells derived from mouse embryonic stem cells被引量：14收藏
- 作者：Xu, Chenze Mohsin, Ali Luo, Yanxia Xie, Lili Peng, Yan Wang, Qizheng Hang, Haifeng Zhuang, Yingping Guo, Meijin
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：STEM CELL RESEARCH & THERAPY 2019
- 关键词：Embryonic stem cells Embryonic Sertoli cells Lentiviral transduction Molecular mechanism Gonadogenesis Male determinant factors
- 质疑
- Unveiling the mechanism of efficient β-phenylethyl alcohol conversion in wild-type Saccharomyces cerevisiae WY319 through multi-omics analysis被引量：13收藏
- 作者：Yang, Chenghan Ren, Yilin Ge, Lihao Xu, Wenting Hang, Haifeng Mohsin, Ali Tian, Xiwei Chu, Ju Zhuang, Yingping
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY JOURNAL 2024
- 关键词：high-yield mechanism high-yielding strain multi-omics respiratory metabolism Saccharomyces cerevisiae beta-phenylethanol
- 质疑
- Co-culture with TM4 cells enhances the proliferation and migration of rat adipose-derived mesenchymal stem cells with high stemness被引量：12收藏
- 作者：Luo, Yanxia Mohsin, Ali Xu, Chenze Wang, Qizheng Hang, Haifeng Zhuang, Yingping Chu, Ju Guo, Meijin
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：CYTOTECHNOLOGY 2018
- 关键词：adipose-derived mesenchymal stem cells TM4 cells Co-culture mode Cell proliferation and migration Stemness
- 质疑
