李志敏 收藏
( 任职于 生物工程学院)
生物工程专业：博士生导师/硕士生导师、生物化工专业：博士生导师/硕士生导师、生物工程领域：工程硕士生导师 工学博士，教授，博导。生物反应器工程国家重点实验室固定研究人员。近年来以第一作者或并列通讯作者在Microbial Cell Factories，Bioresource Technology，Biomass and Bioenergy，Journal of Biotechnology等本领域重点期刊上发表SCI收录论文近20篇。承担和参与了多项科技部“863”项目、上海市科技创新行动计划、国家自然科学基金项目等，同时与国内生物企业建立了紧密的产学研合作关系... 详细>>
- Production of Succinate from Acetate by Metabolically Engineered Escherichia coli被引量：90收藏
- 作者：Li, Yunjie Huang, Bing Wu, Hui Li, Zhimin Ye, Qin Zhang, Y-H Percival
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol;Virginia Tech;Chinese Acad Sci
- 来源：ACS SYNTHETIC BIOLOGY 2016
- 关键词：acetate succinate metabolic engineering Escherichia coli malic enzyme citrate synthase
- 质疑
- Improved succinic acid production in the anaerobic culture of an Escherichia coli PflB ldhA double mutant as a result of enhanced anaplerotic activities in the preceding aerobic culture被引量：89收藏
- 作者：Wu, Hui Li, Zhi-Min Zhou, Li Ye, Qin
- 机构：E China Univ Chem Technol
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2007
- 质疑
- Systematic manipulation of glutathione metabolism in Escherichia coli for improved glutathione production被引量：85收藏
- 作者：Zhang, Jing Quan, Cong Wang, Cheng Wu, Hui Li, Zhimin Ye, Qin
- 机构：E China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol
- 来源：MICROBIAL CELL FACTORIES 2016
- 关键词：Escherichia coli Glutathione Systematic manipulation Bifunctional glutathione synthetase Fed-batch fermentation
- 质疑
- Simultaneous production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol by a recombinant strain of Klebsiella pneumoniae被引量：84收藏
- 作者：Huang, Yanna Li, Zhimin Shimizu, Kazuyuki Ye, Qin
- 机构：E China Univ Sci & Technol;Kyushu Inst Technol
- 来源：BIORESOURCE TECHNOLOGY 2012
- 关键词：3-Hydroxypropionic acid 1,3-Propanediol Klebsiella pneumoniae Aldehyde dehydrogenase
- 质疑
- Co-production of 3-hydroxypropionic acid and 1,3-propanediol by Klebseilla pneumoniae expressing aldH under microaerobic conditions被引量：67收藏
- 作者：Huang, Yanna Li, Zhimin Shimizu, Kazuyuki Ye, Qin
- 机构：E China Univ Sci & Technol;Kyushu Inst Technol
- 来源：BIORESOURCE TECHNOLOGY 2013
- 关键词：3-Hydroxypropionic acid 1,3-Propanediol Klebsiella pneumoniae Aldehyde dehydrogenase Aeration
- 质疑
- Central pathway engineering for enhanced succinate biosynthesis from acetate in Escherichia coli被引量：62收藏
- 作者：Huang, Bing Yang, Hao Fang, Guochen Zhang, Xing Wu, Hui Li, Zhimin Ye, Qin
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol;China Natl Light Ind Council
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2018
- 关键词：acetate cofactor engineering Escherichia coli metabolic engineering succinate
- 质疑
- ATP-free biosynthesis of a high-energy phosphate metabolite fructose 1,6-diphosphate by in vitro metabolic engineering被引量：62收藏
- 作者：Wang, Wei Liu, Meixia You, Chun Li, Zhimin Zhang, Yi-Heng Percival
- 机构：East China Univ Sci & Technol;Chinese Acad Sci;Shanghai Collaborat Innovat Ctr Biomfg Technol
- 来源：METABOLIC ENGINEERING 2017
- 关键词：Fructose 1,6-diphosphate In vitro metabolic engineering Sugar phosphate Hyperthermophilic enzyme Enzyme cocktail
- 质疑
- Development of succinic acid production from corncob hydrolysate by Actinobacillus succinogenes被引量：54收藏
- 作者：Yu, Jie Li, Zhimin Ye, Qin Yang, Yong Chen, Shulin
- 机构：E China Univ Sci & Technol;Chinese Acad Sci
- 来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2010
- 关键词：Actinobacillus succinogenes Succinic acid Corncob hydrolysate Xylose Optimization
- 质疑
- In vitro biosynthesis of ATP from adenosine and polyphosphate被引量：47收藏
- 作者：Sun, Chuanqi Li, Zonglin Ning, Xiao Xu, Wentian Li, Zhimin
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2021
- 关键词：Adenosine triphosphate In vitro Multi-enzymatic cascade catalysis Polyphosphate kinase Adenosine kinase
- 质疑
- Enhanced succinate production from glycerol by engineered Escherichia coli strains被引量：47收藏
- 作者：Li, Qing Wu, Hui Li, Zhimin Ye, Qin
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol
- 来源：BIORESOURCE TECHNOLOGY 2016
- 关键词：Succinate Escherichia coli Glycerol Phosphoenolpyruvate carboxykinase Two-stage fermentation
- 质疑
- Simultaneous saccharification and fermentation of cassava to succinic acid by Escherichia coli NZN111被引量：46收藏
- 作者：Chen, Cuixia Ding, Shaopeng Wang, Dezheng Li, Zhimin Ye, Qin
- 机构：E China Univ Sci & Technol
- 来源：BIORESOURCE TECHNOLOGY 2014
- 关键词：Succinic acid Simultaneous saccharification and fermentation Cassava Escherichia coli NZN111
- 质疑
- Enhanced production of 3-hydroxypropionic acid from glucose via malonyl-CoA pathway by engineered Escherichia coli被引量：45收藏
- 作者：Cheng, Zhuan Jiang, Jiaqi Wu, Hui Li, Zhimin Ye, Qin
- 机构：E China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol
- 来源：BIORESOURCE TECHNOLOGY 2016
- 关键词：3-Hydroxypropionate Acetyl-CoA carboxylase Malonyl-CoA reductase Corynebacterium glutamicum Escherichia coli
- 质疑
- L-Cysteine Production in Escherichia coli Based on Rational Metabolic Engineering and Modular Strategy被引量：43收藏
- 作者：Liu, Han Fang, Guochen Wu, Hui Li, Zhimin Ye, Qin
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol;China Natl Light Ind Council
- 来源：BIOTECHNOLOGY JOURNAL 2018
- 关键词：Escherichia coli L-cysteine modular strategy rational metabolic engineering
- 质疑
- One-pot synthesis of glutathione by a two-enzyme cascade using a thermophilic ATP regeneration system被引量：43收藏
- 作者：Zhang, Xing Wu, Hui Huang, Bing Li, Zhimin Ye, Qin
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2017
- 关键词：Glutathione Bifunctional glutathione synthetase ATP regeneration Polyphosphate Polyphosphate kinase
- 质疑
- Enhancement of Sulfur Conversion Rate in the Production of L-Cysteine by Engineered Escherichia coli被引量：42收藏
- 作者：Liu, Han Hou, Yehua Wang, Yu Li, Zhimin
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2020
- 关键词：Escherichia coli metabolic engineering transcriptional regulator sulfur conversion rate l-cysteine agricultural/food industries
- 质疑
- Heterologous expression and characterization of a novel thermo-halotolerant endoglucanase Cel5H from Dictyoglomus thermophilum被引量：41收藏
- 作者：Shi, Runrun Li, Zhimin Ye, Qin Xu, Jianhe Liu, Yan
- 机构：E China Univ Sci & Technol;Michigan State Univ
- 来源：BIORESOURCE TECHNOLOGY 2013
- 关键词：Endoglucanase Thermostability Halo-tolerance Cellulose binding module Homology modeling
- 质疑
- Biosynthesis of trans-4-hydroxyproline by recombinant strains of Corynebacterium glutamicum and Escherichia coli被引量：40收藏
- 作者：Yi, Yulan Sheng, Huakai Li, Zhimin Ye, Qin
- 机构：E China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomanufacturing
- 来源：BMC BIOTECHNOLOGY 2014
- 关键词：Trans-4-hydroxy-L-proline Recombinant Corynebacterium glutamicum Recombinant Escherichia coli Proline 4-hydroxylases
- 质疑
- Characterization of a new lysine decarboxylase from Aliivibrio salmonicida for cadaverine production at alkaline pH被引量：40收藏
- 作者：Kou, Fengyu Zhao, Jing Liu, Jiao Shen, Jie Ye, Qin Zheng, Ping Li, Zhimin Sun, Jibin Ma, Yanhe
- 机构： State Key Laboratory of Bioreactor Engineering; Key Laboratory of Systems Microbial Biotechnology; Tianjin Institute of Industrial Biotechnology
- 来源：Journal of Molecular Catalysis B: Enzymatic 2016
- 关键词：Alkalinity - Amino acids
- 质疑
- Enhanced 1,3-propanediol production by supply of organic acids and repeated fed-batch culture被引量：39收藏
- 作者：Xue, Xuedong Li, Wei Li, Zhimin Xia, Yuelan Ye, Qin
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2010
- 关键词：1,3-Propanediol Organic acid mixture Repeated fed-batch culture Klebsiella pneumoniae
- 质疑
- Production of glutathione using a bifunctional enzyme encoded by gshF from Streptococcus thermophilus expressed in Escherichia coli被引量：37收藏
- 作者：Li, Wei Li, Zhimin Yang, Jianhua Ye, Qin
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2011
- 关键词：Glutathione Bifunctional glutathione synthetase Feedback inhibition ATP regeneration system
- 质疑
- Improved succinic acid production in the anaerobic culture of an Escherichia coli pflB ldhA double mutant as a result of enhanced anaplerotic activities in the preceding aerobic culture被引量：35收藏
- 作者：Wu, Hui Li, Zhi-Min Zhou, Li Ye, Qin
- 机构： State Key Laboratory of Bioreactor Engineering; State Key Laboratory of Bioreactor Engineering
- 来源：Applied and Environmental Microbiology 2007
- 关键词：Cell culture - Enzyme activity - Genetic engineering - Glucose - Metabolism - Mutagenesis
- 质疑
- Succinic acid production and CO2 fixation using a metabolically engineered Escherichia coli in a bioreactor equipped with a self-inducing agitator被引量：35收藏
- 作者：Wu, Hui Li, Qing Li, Zhi-Min Ye, Qin
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresource Technology 2012
- 关键词：Escherichia coli - Bioconversion - Bioreactors - Ethanol - Fermentation
- 质疑
- Enhanced production of human epidermal growth factor under control of the phoA promoter by acetate-tolerant Escherichia coli DB15 in a chemically defined medium被引量：35收藏
- 作者：Zhang, Yanjun Li, Zhimin Ye, Qin
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Biotechnology and Bioprocess Engineering 2010
- 关键词：Chlorine compounds - Gene expression - Escherichia coli - Dissolved oxygen
- 质疑
- Fitness of Chassis Cells and Metabolic Pathways for L-Cysteine Overproduction in Escherichia coli被引量：35收藏
- 作者：Liu, Han Wang, Yu Hou, Yehua Li, Zhimin
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2020
- 关键词：Escherichia coli l-cysteine metabolic engineering chassis cells
- 质疑
- Simultaneous production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol by a recombinant strain of Klebsiella pneumoniae被引量：34收藏
- 作者：Huang, Yanna Li, Zhimin Shimizu, Kazuyuki Ye, Qin
- 机构： State Key Laboratory of Bioreactor Engineering; Department of Bioscience and Bioinformatics
- 来源：Bioresource Technology 2012
- 关键词：Escherichia coli - Batch cell culture - Aldehydes - Cloning - Gene expression
- 质疑
- Enhanced anaerobic succinic acid production by Escherichia coli NZN111 aerobically grown on gluconeogenic carbon sources被引量：33收藏
- 作者：Wu, Hui Li, Zhi-min Zhou, Li Xie, Jingli Ye, Qin
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Enzyme and Microbial Technology 2009
- 关键词：Sodium bicarbonate - Enzymes - Molecular biology - Cells - Carbon - Glucose - Cytology
- 质疑
- Metabolic engineering of Escherichia coli carrying the hybrid acetone-biosynthesis pathway for efficient acetone biosynthesis from acetate被引量：32收藏
- 作者：Yang, Hao Huang, Bing Lai, Ningyu Gu, Yang Li, Zhimin Ye, Qin Wu, Hui
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol;China Natl Light Ind Council;Chinese Acad Sci
- 来源：MICROBIAL CELL FACTORIES 2019
- 关键词：Acetate Acetone Metabolic engineering Escherichia coli Gas-stripping Resting cell
- 质疑
- Heterologous expression and characterization of a novel thermo-halotolerant endoglucanase Cel5H from Dictyoglomus thermophilum被引量：31收藏
- 作者：Shi, Runrun Li, Zhimin Ye, Qin Xu, Jianhe Liu, Yan
- 机构： State Key Laboratory of Bioreactor Engineering; Biosystems and Agricultural Engineering
- 来源：Bioresource Technology 2013
- 关键词：Cloning
- 质疑
- Production of succinate from simply purified crude glycerol by engineered Escherichia coli using two-stage fermentation被引量：31收藏
- 作者：Li, Qing Huang, Bing He, Qiaofei Lu, Jingxian Li, Xun Li, Zhimin Wu, Hui Ye, Qin
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol;China Natl Light Ind Council;Nanjing Forestry Univ
- 来源：BIORESOURCES AND BIOPROCESSING 2018
- 关键词：Escherichia coli Succinate Crude glycerol Activated carbon Two-stage fermentation
- 质疑
- Cultivation of recombinant Escherichia coli for secretory production of human epidermal growth factor under control of PL promoter被引量：30收藏
- 作者：Zhang, Haiyi Li, Zhimin Qian, Yue Zhang, Qian Du, Peng Gan, Renbao Ye, Qin
- 机构： State Key Laboratory of Bioreactor Engineering; Institute of Biochemistry and Cell Biology
- 来源：Enzyme and Microbial Technology 2007
- 关键词：Cell culture - Enzymes - Genes - Nutrition - Thermal effects
- 质疑
